Objective To investigate ?-cell early insulin secretion function and insulin resistance in different durations and grades patients with hypertension.Methods One hundred and six middle and old age(58.4?8.5) years hypertension patients were enrolled.According to hypertension durations,patients were categorized as ≤1 year group(n=35),1.1-4.9 years group(n=34),≥5 years group(n=37);while based on grades of hypertension,patients were divided as 1st grade(140-159/90-99 mmHg,n=53),2nd grade(160-179/100-109 mmHg,n=30),3rd grade(≥180/≥110 mmHg,n=23).Plasm glucose,HOMA-IR,?I30/?G30 were determined and compared among groups.Results Patients with longer duration(≥5 years) had most seriously impairment of ?-cell early secretion(?I30/?G30)[≥5 years(2.1?4.5) vs 1.1-4.9 years(10.9?4.2),vs ≤1 year(10.3?4.2),all P

Objective To observe the effects of anti lipopolysaccharide (LPS) immunoglobulin Y (IgY) on LPS in vitro . Methods The direct in vitro neutralization of different concentrations of IgY and LPS was conducted for the detection of the remained amount of LPS to observe the dose effect relationship. The primary cultured human umbilical vein endothelial cells (HUVECs) were cultured for 48-72 h after addition of certain concentrations of fluorescein isothiocyanate LPS (FITC LPS) and IgY. Changes of the fluorescent intensity in HUVECs were observed at different time points. After addition of certain concentrations of LPS and IgY for incubation with HUVECs for 4, 8, and 12 h, the changes of lactate dehydrogenase (LDH) activity were measured. Results The dose effect relationship was satisfactory when the ratio was 16︰1. At 4, 8, and 12 h, fluorescent intensity was somewhat strong in FITC LPS group, but weak in IgY group, especially at 8 and 12 h. The LDH activity in medium increased obviously after incubation for 8 h in LPS group ( P

At present, due to the opening and circulation of salt market, potassium iodate iodized salt, potassium iodide iodized salt, seaweed iodized salt and non-iodized salt may exist simultaneously in the edible salt market of a region, which brings new problems to the work of testing iodine content of edible salt in the laboratory. In order to carry out salt iodine detection for prevention and monitoring of iodine deficiency disorders, this paper introduces some methods which established by the author for a rapid qualitative, semi-quantitative and quantitative detection of iodized salt of different forms of iodine and non-iodized salt. And further application and discussion of the methods are described for reference of iodized salt monitoring.

Objective To explore the conversion rate of iodate ions (IO3-) being reduced to iodide ions (I-) by ascorbic acid (C6HsO6,VC) in simulated human gastric juice,and to provide references for further safety evaluation of edible salt iodized with potassium iodate.Methods An ion chromatography method was developed to detect iodide ions in simulated gastric juice.The conversion rate of iodate ions being reduced to iodide ions was used as the index for scavenging rate of iodate ions.In simulated gastric juice in vitro and in 37 C water bath,the scavenging effects of VC on iodate ions were determined in simulated gastric juice with different VC concentrations and simulated gastric juice acidities,as well as for different reaction time.Ion chromatography column:Dionex IonPac AS19 (250 mm × 4.0 mm);eluent:KOH 30 mmol/L (online produced),isocratic eluting,flow rate 1.0 ml/min,injection volume 100 μl,and detected by a conductivity detector.Results Performance of the method:within the range of 0-5 000 μg/L,iodide ions concentration and the chromatographic peak area had a good linearity (correlation coefficient r =0.999 7),and the detection limit of iodide ions was 20 μg/L.For quantification of iodide ions in simulated gastric juice,the relative standard deviation (RSD) of repeated measure for 6 times was ＜ 2.0％,the standard addition recovery rate was 97.6％-102.4％,and the overall average recovery rate was 99.4％.In the simulated gastric juice with a pH of 1.4 containing 5 mg/L and ≥ 10 mg/L VC,the reaction time to achieve 100％ conversion rate of iodate ions being reduced to iodide ions was 5 min and 2 min,respectively.In the simulated gastric juice with a pH of 3 containing 10 mg/L ascorbic acid,the reaction time to achieve 100％ conversion rate was 15 min.VC quantitatively reduced iodate ions to iodide ions by the stoichiometric relationship between reactants of the reduction reaction equation,and every 100 μg VC quantitatively reduced 24.0μg of iodine in iodate to iodide ions.Conclusions In simulated gastric juice,the reaction of iodate ions being reduced to iodide ions by VC is a stoichiometric reaction with relatively fast reaction rate,the scavenging rate of iodate ions by VC within the concentration level in human gastric juice can reach 100％.The results prompt that the iodate ions from edible salt iodized with potassium iodate in daily diet are reduced to iodide ions mainly in the human stomach.

Objective To resolve the problem that qualified chloramine T was difficult to purchase for some basic laboratories Methods Using the solution of available chlorine prepared by bleaching power instead of chloramine T, the method for determination of cyanide in drinking water was improved Results The data obtained by this assay accorded with Beers Law in the range of 0～2 0 ?g/25 ml cyanide The detection limit,relative standard deviation,and recovery rate were 0 04?g,2 69%～3 09% and 97 50%～99 17% respectively Conclusion This method for determination of cyanide in drinking water was simple and easy to operate and presented good precision and accuracy

Objective To study the gene expression and the expression change rule of c-fos in the canines posterior to craniocerebral gunshot wounds in order to provide the experimental base for further understanding of the mechanism of gunshot craniocerebral injury. Methods Using the canine model of penetrating craniocerebral injury made by the bullets of the small-calibre rifle made in Germany, the c-fos expressions in the control cerebral tissues and the contusion and concussion areas of the trajectory and the brain stem neurons at different time points after trauma were observed by immunohistochemical method. Results Feeble c-fos expression was detected in the control group. After craniocerebral gunshot wounds, the c-fos expression increased in the contusion and concussion areas of the trajectory and in the brain stem at the 30th minutes, reached the peak at the 2nd hour and began to decrease at the 3rd hour. The c-fos expression in the concussion area was much more significant than that in the contusion area ( P

Objective To study the change in blood-brain barrier (BBB) permeability in dog with craniocerebral gunshot injury and its significance. Methods Twelve dogs were randomly divided into control group, 15min ofter penetrating craniocerebral injury (PCI) group, 1h after PCI group and 6h after PCI group (n=3). The injury was produced by a small-calibre rifle made in Germany (type 5.56mm, weight of bullet 2.57g). Lanthanum nitrate was infused through the heart. The changes in BBB permeability were observed under electronic microscope. Results In 15min PCI group and 1h PCI group, changes in BBB permeability was observed, with manifestation of opening of tight junctions, with lanthanum nitrate particles passing through BBB into the brain tissues. These changes were more marked in 1h PCI group. Comparing with the above two groups, even more lanthanum nitrate particles were found in the brain tissue in the 6h group (P

Objective To investigate the cause of refractory hiccups. Method 34 patients with refractory hiccups admitted to our hospital from January 1996 to December 2003 were examined with gastroscopy. The correlation of hiccups with reflux esophagitis and the efficacy of omeprazole treatment were studied. Results 10 patients were proven endoscopically to have esophagitis and duodenal ulcer, and the other 24 were suffering from esophagitis. According to Tokyo classification, 14 cases were having grade III esophagitis, and 20 were grade IV. All patients received omeprazole 20mg twice daily and cisapride 10mg thrice daily, and hiccups disappeared after 3 days. Conclusion Refractory hiccups may be the initial or primary symptom in moderate-severe esophagitis in aged people. Proton pump inhibitor therapy is effective in stopping refractory hiccup.

Objective To observe the characteristics of obesity in rats with metabolic syndrome (MS) and to study the role of ERK1/2 and p38MAPK in the production of adipose tissues. Methods The metabolic parameters, weight of adipose tissue, waist circumference (WC), Lee's index, distribution area of adipocytes and the number of big adipocytes in the rats were measured. Adipose tissues and liver were histopathologically observed. ERK1/2 and p38MAPK of adipose tissue were determined using Western blot. Results (1) The blood pressure, fasting insulin level, LDL-C, TG and FFA in rats with MS were markedly higher than that in control rats (P