In view of the present hospital professional and technical personnel in the making , performance e-valuation , salary management , personal development , health management , department management , as well as on the management of some of the ethical issues , analyzes its reason: hospital professional and technical personnel management ethics idea misunderstanding , hospital professional and technical personnel subject ethics value status unclear , hospital professional and technical personnel managers′ethical level is not high .And put forward the con-crete solving measures:strengthen dominated by social responsibilities of ethical supervision and management , es-tablish the principle of fairness , justice , ethics of competition system , director of the department , carried out by both efficiency and fair ethics idea of performance and salary rank management activities , to achieve individual all-round development as the goal of ethical career management , to strengthen the management of managers ethical education .

The purpose of this investigation is to evaluate the excitability of the sympathetic nerves in burned rats. Pre ganglionic discharges from the cervical and the epinephronic sympathetic nerves and post-ganglionic discharges from the renal sympathetic nerve fibers were recorded in rats anaesthized with urethane. After burning, the discharges of the sympathetic nerves of the rats at first showed continuous high frequency soon followed by a decline. After that, the sympathetic efferent electrical activity became very active again, displaying a high amplitude and high frequency.These results suggest that the sympathetic nerves system exhibits periodic excitatory changes in burned rats with a excitation-inhibition-excitation cycle.

As master genes during embryogenesis, Homeobox(Hox) genes play important roles not only in embryonic development, cellular growth and differentiation and the expression of the specific genes of some tissue, but also in controlling hematopoietic tissue development and differentiation in a germ line -specific and stage-specific. At present studies showed there is a close relationship between Hox genes and tumorgenesis. This article reviews the advances in the research on correlation between homeobox genes and tumorgenesis.

BACKGROUND:Genetic modification by Shootin1 aims to effectively improve neural differentiation of bone marrow mesechymal stem cel s (BMSCs) in the injured spinal cord, thereby promoting functional recovery from spinal cord injury after cel transplantation. OBJECTIVE:To explore the nerve regeneration ability of transplanted BMSCs overexpressing Shootin1 in rats with spinal cord injury. METHODS:BMSCs were transfected using adenovirus-Shootin1 for 48 hours. Then, immunofluorescence staining was used to detect Nestin and NeuN expression levels in the transfected cel s under in vitro neuronal induction and differentiation. Animal models of spinal cord injury were made in rats using modified Al en’s method. Thirty minutes later, Shootin1-transfected BMSCs and non-transfected BMSCs were respectively injected into the subarachnoid space of the rats in the transfection and non-transfection groups, respectively. Rats in the model group were given no treatment. Five weeks after modeling, spinal cord samples were taken from each rat to make frozen sections for detection of nerve related markers RESULTS AND CONCLUSION:After 48-hour adenoviral transfection, Shootin1 expression was successful y detected in BMSCs. After 7-day in vitro induction, the cel morphology in the three groups varied, and there was no significant difference in the expression of Nestin and NeuN between the transfection and non-transfection groups. Basso, Beattie and Bresnahan scores were higher in the two cel transplantation groups than the model group. Increased expression levels of Nestin, NeuN, GFAP, MAP-2, ChAT and SYN were observed in both two cel transplantation groups, indicating a strengthened ability of nerve regeneration. Our experimental findings further confirm that BMSCs transplantation for spinal cord injury has achieved good outcomes, and Shootin1 protein plays a certain role in nerve regeneration and functional recovery after spinal cord injury. However, Shootin1 overexpression shows no obvious additional effects in combination with BMSCs transplantation, and further studies on the optimization of BMSCs transplantation for spinal cord injury are necessary.

An animal model of acute lung injury was reproduced by intravenous injection of E. coli endotoxin to burned rats. The histological changes of lung were studied using transmission electron microscope. It was found that leukocytes accumulated in the pulmonary capillaries and adhered to endothelia. The WBC count decreased gradually. The level of MDA, lung coefficient and lung water content were increased. The SOD activity was decreased. Pretreatment with SOD could improve the changes mentioned above. This study suggests that oxygen radicals play an important role in acute lung injury induced by burns complicated by endotoxemia.

To understand the antagomism of Clostridium butyricm and Bifidobacterium infants to v. cholera. Either Clostridium butyricum LCL166 or Bifidobacterium infants LCL172 or both of them co-cultured with V. cholera of, 0139, counting the number of V. cholera in constant intervals and performing statistics analysis. The inhibition results are probably same when combination of LCL166 and LCL172 were compared with LCL166 alone. The antagonism to V. cholera results mainly from Clostridium butyricum LCL166 which has the inhibition effect to V.cholera.

Objective To investigate the mechanism of regulation of endothelium participating angiogenesis by macrophage. Methods Human umbilical vein cell line (ECV-304) was cultured. When 60% of the ECV-304 cells became confluent, human macrophage cell line (U937) after being stimulated by concanavalin A (Con A) was co-cultured with them. The cells were then divided into four groups, i.e. control group (pure ECV-304 culture), co-culture group of Con A with ECV-304, co-culture group of U937 with ECV-304, and co-culture group of U937 with Con A and ECV-304. After 48 hours of co-culture, the morphology of ECV-304 cells was observed under invert microscope, and the changes in cell cycle of ECV-304 were determined by flow cytometry. In addition, the changes in the expression levels of homeobox (HOX) B2 mRNA and VEGF receptor KDR of endothelia cells were assessed by RT-PCR. Results In ECV-304 co-cultured with Con A and U937 group, inter-endothelial gaps were found to be widened, cell shape became abnormal with obvious processes simulating neurons, and the endothelial S phase was prolonged significantly (P

Objective To explore the role of active macrophages on the vascular endothelial participation in angiogenesis. Methods Vasculuar endothelial cell line ECV-304 was inoculated and cultured to 60% confluence.ECV-304 cells were then cultured lonely, with ConA(24?g/ml), with macrophage cell line U937(1?10 5/ml) or with U937 and ConA together to make four groups.The change of the ECV-304 cell cycle was determined by flow cytometry and that of the DNA synthesis was detected by 3H-TdR incorporation test. The change of the expression of endothelial VEGF receptor KDR mRNA was detected by RT-PCR while that of the integrin receptor ? ? ? 3 expression by immune fluorescent antibody technique on flow cytometry. Results Endothelial cells in S phase increased obviously with increased DNA synthesis in ECV-304 cells stimulated by active macrophages. In addition, the expression levels of the VEGF receptor KDR mRNA and integrin receptor ? ? ? 3 up-regulated significantly(P

Objective To characterize the effects of Sanchi on the alcoholic hepatopathy rats.Methods 70 SD male rats were randomly divided into five groups:normal group(n=10),control group(n=15),high-dose Sanchi group(n=15),low-dose Sanchi group(n=15),and Tiopronin group(n=15).In order to induce alcoholic hepatopathy,animals in the control,high-dose and low dose Sanchi,and Tiopronin groups were orally dosed with HongXing ErGuoTou(56% ethanol;5g/kg),corn oil(2ml/kg),and pyrazole(27.2 mg/kg)every morning for 14 weeks.Rats in high-dose Sanchi,low dose Sanchi,and Tiopronin groups,were treated every afternoon during the 14-week induction of hepatopathy,with 1.2g/kg Sanchi,0.6g/kg,and 100mg/kg Tiopronin,respectively.All rats were sacrificed 12 hours following the last injection.Blood samples were obtained prior to euthanization.Blood-fat and serum lipoidase activity were measured using an automatic blood biochemical analyzer.Blood haluronic acid(HA),laminin(LN)were determined by ELISA.Histopathological changes of hepatic tissues(i.e.steatosiss,inflammation,and fibrosis)were assessed by microscopic examination of HE and Masson staining of the right lobe of livers.Results Compared with rats from the normal group(treated with ddH2O),rats in the control group have significantly higher steatosis,inflammation,liver fibrosis,and increased level of serum cholesterol,HDL-C,LDL-C,haluronic acid,laminin and AST/ALT activity,(P0.05).More importantly,treatment with high-dose,low-dose Sanchi,or Tiopronin resulted in less severe steatosis,inflammation,fibrosis in the liver,and reduced the levels of serum haluronic acid,laminin(P0.05).Conclusions(1)Daily dosing of alcohol,corn oil,and pyrozole for 14 weeks faithfully replicated the alcoholic hepatopathy symptoms observed in the ALD model,such as hepatic steatosis,immune cell infiltration,blood-fat disturbance,increase in serum AST/ALT activity,and liver fibrosis.(2)Sanchi can significantly alleviate the symptoms in the alcoholic hepatopathy rats including steatosis,improve hepatic function and fibrosis.