In order to sum up the antineoplasmic active components of Holothuria, we studied the literatures in recent years. The results showed that Holothuria contained a variety of anti-tumor active ingredient, such as Holothurian Glycosaminoglycan and Holothurin. These anti-tumor active components played important roles in different stages of tumor developmment, progression and metastasis. The discovery of new anti-tumor active ingredients may be helpful for the application of Holothuria in clinical tumor treatment.

The deposition of amyloid β-protein (Aβ is the key pathological basis of cerebral amyloid angiopathy (CAA) and Alzheimer's disease (AD). The current studies emphasis that the stability of internal environment between cells of the neurovascular unit is the key to maintain normal brain function. This article reviews the relationship between the Aβand the neurovascular unit injury, and we expect to provide some new ideas for the clinical prevention and treatment of CAA and AD.

BACKGROUND: It has been found that hypoxia inducible 1α (HIF1α) gene can improve the ability of tissues and cells to survive in the ischemic environment, which is of great significance in promoting blood vessel regeneration.OBJECTIVE: To investigate the effect of the recombinant adenovirus Ad-CMV-HIF1αmu-IRES-hrGFP-1 on the proliferation of bone marrow mesenchymal stem cells.METHODS: Passage 3 bone marrow mesenchymal stem cells were cultured and transfected with Ad-CMV-HIF1αmu-IRES-hrGFP-1 virus (experimental group), with non-mutated gene Ad-CMV-HIF1α-IRES-hrGFP (positive control group), with Ad-CMV-IRES-hrGFP-1 empty virus (negative control group), or with no virus solution (blank control group). After transfection for 24 hours, the expression of HIF1α mRNA and protein was detected. Cell proliferation was detected by MTT assay at 24, 48, 72, 96 and 120 hours after transfection.RESULTS AND CONCLUSION: (1) Except that the mRNA expression of HIF1α in the experimental group and the positive control group was significantly higher than that in the negative control group and the blank control group (P < 0.05), there was no significant difference between two groups. (2) The expression of HIF1α protein in the experimental group was significantly higher than that in the positive control group, the negative control group and the blank control group (P < 0.05), and there was no significant difference between the latter three groups. (3) Cell proliferation was faster in the experimental group than the positive control group and the blank control group (P < 0.05), and moreover, there was no significant difference between the latter two groups. Our experimental findings indicate that the recombinant adenovirus-mediated three-point mutant HIF1α combined with hrGFP gene transfected bone marrow mesenchymal stem cells could not only express the target protein, HIF1α, under normoxic conditions, but also promote the proliferation of bone marrow mesenchymal stem cells.

Objective Toobservetheclinicalsignificanceofcerebrovascularhemodynamicindex (CVHI)inthepatientswithsilentcerebralinfarction.Methods Atotalof180patientsperformed cerebrovascular hemodynamics with ultrasound and head MRI examinations were enrolled retrospectively. They were divided into three groups:60 patients with silent cerebral infraction (SCI)were used as a SCI group,60 age-,sex- and previous medical history-matched high risk patients with cerebrovascular disease were used as a cerebrovascular disease risk factor (CV-HRF)group,and 60 healthy subjects were used as a normal control group over the same period. Cerebrovascular function detector was used to collect CVHI at the bilateral carotid arteries. The CVHI features of the 3 groups were analyzed and compared. Results Comparedwiththecontrolgroup,thereweresignificantchangesinvariousindexesofcerebral vascular hemodynamics in the SCI group and the CV-HRF group. The indexes of reflecting the cerebral blood supply state included significantly decreased mean blood flow,mean flow velocity,maximum flow velocity,and minimum flow velocity (P<0. 05);the indexes reflecting vascular elastic properties and resistance status included the increased pulse wave velocity,characteristic impedance,peripheral resistance,and dynamic resistance (P<0. 05);the indexes reflecting cerebral microcirculation included significantly increased critical pressure and decreased differential pressure (P<0. 05);the total score of cerebrovascular function was also decreased significantly,they were 44 ± 9,51 ± 5,and 85 ± 7,respectively (P<0. 05);there were significance differences in the distribution of total score amongthe3groups(P<0.05).Conclusion Thehigh-riskpatientswithsilentcerebralinfarction and cerebrovascular disease have the CVHI changes. The total integrated value of cerebrovascular function declined,and the former is more obvious.

Objective To investigate the effect of quantifying health education on nurse teaching in cardiovascular department. Methods Totally 98 nursing college students practicing in cardio-vascular department were randomly divided into experimental group and control group . Method of quantifying health education was employed in experiment group while traditional education was used in control group;all patients were guided by health education. After the internship, two ques-tionnaires were used separately to evaluate the health education effects on nurse students and patients. SPSS 17.0 software was used for statistical processing. The measurement data were expressed as x±s; The mean between the groups was compared using t test; the enumeration data between the groups were com-pared using字2 test, the ranked data were compared using rank sum test. α=0.05 was taken as inspec-tion level. Results The health education cognitive scores were higher in experimental group than in control group (P<0.05). The health education ability scores, theoretic test scores, and operation test scores were higher in experimental group than in control group, with statistical differences (t=3.20, 2.10, 2.87; P=0.001, 0.032, 0.004). There were statistical differences in patients' cognition after receiving health education. (Z=13.435, 14.290, 12.901, 12.466, 12.168, 11.350; all P=0.000). Conclusions Quantification of health education can improve health education cognition and ability of nursing students in cardiovascular department, enhance the ef-fectiveness of clinical practice, and help patients master knowledge of health education.

Objective To make a survey of the binocular visual acuity, residual visual acuity and the pathogenic factors causing the deficient of the vision handicap student in Blindness-Deaf-Mute school in Guiyang to provide the data for low vision rehabilitation. Methods Through routine examination, we examined the 58 students with vision impairment and further advised them wear the optics visual aids. Results 6 cases of low vision, accounting for 10.3%; 52 cases of blindness, accounting for 89.7%. One case was rectified its visual acuity by means of subjective refraction and rectification of eyesight. Causes of the disease: 35 cases suffered from the deficient due to genetic factors, accounting for 60.3%; among them, the congenital glaucoma was the first (12/35). 23 cases suffered from the deficient due to acquired factors, accounting for 39.7%, among them, the keratomalacia was the first (19/23). Visual aids wearing :visual acuity≥0.3, 3 cases; visual acuity≥0.05, 6 cases. Conclusions Prevention can prevent the genesis of blindness. The students of residual visual acuity should wear visual aids.

OBJECTIVE:To find out the main problems about quality supervision over Chinese herbal pieces and effective su-pervision strategies. MEDTHODS:First,static and dynamic models of the game between the enterprises engaged in the production and business of Chinese herbal pieces and local food and drug administration were respectively established,to which pure strategy Nash equilibrium analysis and mixed strategy Nash equilibrium analysis were made. Next,the model of the game between the supe-rior regulatory authorities and the basic-level regulatory authorities was established,to which mixed strategy analysis was made to find out the supervision factors relevant to the quality safety of Chinese herbal pieces. RESULTS & CONCLUSIONS:Based on the current situation of quality supervision over Chinese herbal pieces and the analysis results of the game models,it is suggested that at present the Chinese herbal medicine regulatory authorities of China should work hard to promote the reform of the supervision system,reduce supervision cost(including the guidance of the large-scale and centralized production of Chinese medicinal materi-als and unified appliance of approval number to the administration of Chinese herbal pieces),establish a systematic and comprehen-sive system of rewards and punishments,create a professional supervisor staff(including the strengthening of basic-level supervisor staff creation and the improvement of personnel's quality,the standardization of the law enforcement of regulatory authorities and the combination of proper power delegation to lower levels and strict supervision),and balance the relationship between the govern-ment and the public in supervision to increase the public's awareness of responsibility.

OBJECTIVETo investigate the changes on the number and distribution of myoepithelial cells (MECs) during parotid gland regeneration.

METHODSA total of 54 Wistar rats were divided into eight experimental groups and one normal control group, with six rats in each group. The right parotid ducts of the rats in the experimental groups were ligated for 14 days and then reopened. The parotid tissue specimens were harvested at days 0, 1, 3, 5, 7, 10, 14, and 21. The histological changes of the regenerating gland were examined using hematoxylin-eosin staining. Immunohistochemical labeling was also performed to investigate the changes in the number and distribution of MECs at different time points of parotid regeneration. Both the histological and immunohistochemical changes observed in the experimental groups were compared with those in the normal control group.

RESULTSThe parotid gland showed marked atrophy 14 days after ligation. Most acinar cells disap- peared, but the number of duct-like structures obviously increased. MECs apparently increased in number and were mainly located at the periphery of the duct-like structures. Three days after duct reopening, the number of acinar cells significantly increased and the duct-like structures significantly reduced. Meanwhile, MECs also decreased in number and were mainly located at the periphery of the newly formed acini and duct-like structures. The number of MECs noticeably decreased 3 and 5 days after duct reopening. At 14 days after duct reopening, the glandular structures and the number and distribution of MECs returned to normal compared with those in the normal control group.

CONCLUSIONThe number and distribution of MECs returned to normal condition after parotid gland atrophy. Parotid regeneration mainly occurred within 5 days after duct reopening.

Numb,one of the important cell fate determinants,plays a key role in regulation of nerve system development,and it is closely related to the occurrence of tumors.It is proven that Numb is implicated in the processes of occurrence,invasion and metastasis of tumors through regulating the way of cell division,cell polarity,epithelial-mesenchymal transitions and several signal pathways,e.g.,Notch,Hh,p53.The expression levels of Numb in cells are related to the malignant degree and prognosis of tumors.It is vital to explore the role of Numb in tumors for treating tumors at the standpoint of Numb.

Objective:To investigate the histological changes of parotid gland after ligation and reopening of the main duct.Meth-ods:The right parotid main duct was ligated for 14 days and then reopened,the specimen were harvested at day 0,1,3,5,7,10,14 and 21 after reopening(n=5 )respectively.By HE staining and Morphology measurement,the morphological and volume fraction changes of acinus,duct and mesenchyma were observed and measured.Results:The parotid gland atrophied significantly 14 days af-ter the duct ligation.From day 3 after duct reopening,the acinar volume fraction continued to increase,both the duct and the mesen-chymal tissue volume fraction continued to decrease significantly(P<0.05).After 14 days of duct reopening,there was no signifi-cant difference in volume fraction of acinar,duct and mesenchyma,compared with those of normal control group(P>0.05).Conclu-sion:After reopening of the ligated main duct the atrophied parotid gland is able to regenerate to be normal.