Objective To evaluate the role of PI3K-Akt-HIF-1α signaling pathway in dexmedetomidine-induced reduction of damage to the unventilated lung during one-lung ventilation (OLV) in rats.Methods Forty adult male Sprague-Dawley rats,weighing 250-350 g,were randomly divided into 4 groups (n=10 each) using a random number table:two-lung ventilation TLV) group,OLV + normal saline group (group OLV),OLV + dexmedetomidine group (group D),and OLV + dexmedetomidine +LY294002 group (group DL).Bilateral lungs were ventilated for 2.5 h in group TLV,and the right lung was ventilated for 2 h followed by 0.5 h TLV in the other groups.The rats were sacrificed,the left lung was removed for determination of wet to dry lung weight ratio (W/D ratio),and expression of phosphorylated Akt (p-Akt) and hypoxia-inducible factor-1 alpha (HIF-1α) (by Western blot).Results Compared with group TLV,W/D ratio was significantly increased,the expression of p-Akt was down-regulated,and the expression of HIF-1α was up-regulated in the other three groups (P＜0.05).Compared with group OLV,W/D ratio was significantly decreased,and the expression of p-Akt and HIF-lα was up-regulated in D and DL groups (P＜0.05).Compared with group DL,W/D ratio was significantly decreased,and the expression of p-Akt and HIF-1α was up-regulated in group D (P＜0.05).Conclusion PI3K-Akt-HIF-1α signaling pathway is involved in dexmedetomidine-induced reduction of damage to the unventilated lung during OLV in rats.

Asia ; epidemiology ; Humans ; Non-alcoholic Fatty Liver Disease ; epidemiology ; Prevalence

The deposition of amyloid β-protein (Aβ is the key pathological basis of cerebral amyloid angiopathy (CAA) and Alzheimer's disease (AD). The current studies emphasis that the stability of internal environment between cells of the neurovascular unit is the key to maintain normal brain function. This article reviews the relationship between the Aβand the neurovascular unit injury, and we expect to provide some new ideas for the clinical prevention and treatment of CAA and AD.

DICOM standard is a compulsory international standard for the establishment of Integrated Hospital Information System (IHIS) and Picture Archiving and Communication System (PACS). This paper introduces such information of DICOM3.0 as its generation, components, operational mechanism and application. To understand DICOM standard exactly is helpful for the efficient operation of PACS.

There are many therapeutic drugs for rheumatoid arthritis(RA) treatment,but the pathogenesis of RA have not been fully understood,therefore no curable drug has been found.With increasing knowledge of the inflammatory process,especially in signaling,some drugs modifying disease progress and interfering signaling pathways have been studied.Cytokine inhibitors such as tumor necrosis factor-blocking agents and IL-1 receptor antagonist in clinical trials and in practice have resulted in dramatic improvement in RA disease control.Important molecular mechanisms of actions on total glucosides of paeony(TGP) and Glucosides of Chaenomeles speciosa(GCS) were showed to modulate G protein-AC-cAMP and Ras-MAPKs signaling pathways and to inhibit synoviacytes proliferation.This review focuses on agents which target various cytokines,other inflammatory mediators,antigen of cells,and signaling pathways.

Objective To observe the effect of acupuncture at Back-shu points on chronic fatigue syndrome (CFS).Methods The 45 CFS patients were randomized into two groups. Acupuncture at Back-shu points was used in the treatment group (22 cases), while placebo needling was given to the control group (23 cases). SF-20 and Chalder fatigue scale were used to measure the degree of general health before and after treatment.Results In the treatment group, the scores of SF-20 in physiological functions, social functions, role functions, psychological health, overall health, and pain were significantly increased(P

Objective:To evaluate the short-term and middle-term therapeutic effects and the safety of deep acupuncture at Tianshu(ST 25) for treatment of slow transit constipation(STC).Methods:60 cases of STC were randomly divided into an acupuncture group and a control group.Cleveland Constipation Score(CCS) and colic transit time(CTT) before treatment,2 weeks and 6 months after the treatment were investigated,and the safety was evaluated by clinical,laboratory and imaging indexes.Results:For the short-term therapeutic effect,the treatment group was better than the control group(P

Objective:To investigate the presence of Anti-Neutrophil Cytoplasmic Antibodies(ANCA) and their specific antigens in the patients with thromboangiitis obliterans(Buerger's disease,TAO).Methods:The TAO group includes 23 patients.The arteriosclerosis obliterans(ASO) group includs 25 pantients and the control group is health group includs 25 volunteers.Serum samples were investigated for ANCA prevalence by indirect immunofluorescent technique in TAO group,ASOgroup and health group.the reactivity to proteinase 3(PR3),myeloperoxidase(MPO),cathepsin G(CG),bactericidal/permesbility-increasing protein(BPI),elastase(HLE) and lactoferrin(LF) was measured by ELISA in TAO group and health group.Results:(1) There are 13 of 23 patients with TAO who are tested ANCA positive by indirect immunofluorescence,the ANCA prevalence is 52.2%.Only one patient is pANCA positive among 25 patients with ASO,the prevalence is 4%,and no ANCA are found in healthy controls.(2) ANCA were predominantly found in group II(66.7%;12/18) compared to group I(33.3%;3/9) and the levels of the titre of ANCA in the group II was higher than group I.(3) Only five of 12 ANCA-positive sera reacted with MPO,three of the twelve reacted with LF,one serum is HLE positive and one is BPI positive.No serum reacted with PR3 and CG.(4) Anti-MPO,anti-LF,anti-BPI and anti-HLE autoantibodies were found only in the severe group.Conclusion:The prevalence of ANCA and levels of Anti-MPO,anti-LF,anti-BPI and anti-HLE correlated with disease severity.Therefore,detection of ANCA can be a helpful serological test for the diagnosis of thromboangiitis obliterans.

Objective To study the physiochemical properties of selenium exopolysaccharide from Porphyridium sp.(Se-PSP).Methods The Se-PSP was obtained from ASW,a sort of culture medium added with some selenious acid solution,and was purified by DEAE-52 cellulose column and identified by Sephadex G-200.The acidity,UV spectral absorption,paper chromatography,and gelose electrophoresis of Se-PSP solution were studied.Results and Conclusion Proper concentration of selenious acid in ASW culture had no influences upon the growth of Porphyridium.Se-PSP prepared in this way didn't contain protein and nucleic acid and the solution of Se-PSP showed acidity.It is probable that Se-PSP contain two fractions of PSP.

Objective:To analyze the clinical characteristics of enterococcal infection in leukemia patients. Methods:The clinical characteristics of 11 cases of leukemia with enterococcal infection were summarized by contrast to those of 62 cases of non-leukemia patients with enterococcal infection. The statistical analysis of measurement data applied chi square text. Results: In leukemia group,11 cases (100%) used broad-spectrum antibiotic before enterococcal infection,the length of stay of 8 cases (72.2%) exceeded 15 days before enterococcal infection,9 cases (81.2%) were caused digestive tract infections by enterococci,the clinical manifestations of 10 cases (90.9%) were hyperpyrexia after enterococcal infection,and 9 cases (81.2%) had to use Vancomycin to eliminate enterococci. Whereas in non-leukemia group,there were 22 cases (35.5%),6 cases(9.7%),14 cases (22.6%),9 cases (14.5%) and 9 cases (14.5%) respectively in five aspects above mentioned. The differences between two groups were significant (P0.05). Conclusion:Contrast to non-leukemia patients infected by enterococci,leukemia patients were more easily infected by enterococci because to the long-term of using broad spectrum antibiotic,the long length of stay before enterococcal infection and the weakened power of resistances after chemotherapy. Enterococcal infections were mainly hospital infections,and digestive tract infections were most common. The main clinical manifestations were hyperpyrexia and diarrhea. Enterococci were highly resistant to usual antibiotics but well sensitive to Vancomycin.