Neonatal hypoxic-ischemic encephalopathy(HIE)is a common disease in neonatal nervous system injury,which often treated by the traditional measures such as three support′s and three symptomatic treatments.Moreover,there is no specific treatment nowadays.The human umbilical cord mesenchymal stem cells(HUC-MSC)are mixture of multifunctional stem cells from neonatal umbilical cord tissues,which has strong abilities of self-renewal and multilineage differentiation potential.HUC-MSC are easy to harvest and have more sources,low immunogenicity and without ethical problems.It can differentiate into neural cells under certain conditions,secrete neurotrophic factors,repair the damaged brain and promote the recovery of cerebral function.Transplantation of HUC-MSC provides a new way for the treatment of HIE.

Henoch-Schonlein purpura (HSP) is the most common form of childhood autoimmune vascular inflammatory diseases,the incidence rate showed an increasing trend,its pathogenesis has not yet been fully elucidated.Thl7 cells are recently discovered to be a new kind of different from Th1,Th2 effector of CD4+ T cell subsets,Th17/Treg imbalance is closely related to the pathogenesis of HSP.The paper reviewed the related researches.

Objective To construct RNA interference(RNAi)lentivirus plasmid of eukaryotic elongation factor 2(eEF2)gene and stably transfected breast cancer cell model,so as to provide experimental basis for further study on the role of eEF2in the occurrence and development of breast cancer.Methods According to eEF2 gene sequence and the design principles of short hairpin RNA sequence,three pairs of shRNA sequences were designed and synthesized,which were refolded and inserted into lentivirus vector LV-U6-shRNA-ZSgreen-Puro. Three recombinant plasmids sh1,sh2 and sh3 with different knock-down targets of eEF2 gene were constructed,and empty plasmid vectors were used as negative control group(shNC). Human renal epithelial cells HEK293T were co-transfected with three lentivirus plasmid packaging system for virus packaging and amplification respectively. After identification by digestion and sequencing,the recombinant lentivirus was used to infect breast cancer cells MCF-7 in logarithmic growth period. After 72 h,the green fluorescence intensity was observed under a fluorescence microscope,the mRNA transcription level of eEF2 was determined by fluorescence quantitative PCR(qPCR),and the expression level of eEF2 protein was detected by Western blot.Results The sequence of eEF2shRNA vector was identical to the original sequence. The expression of green fluorescent protein was observed in breast cancer cells MCF-7 infected with recombinant lentivirus. Compared with shNC group,the mRNA transcription levels of eEF2 in sh2 and sh3 group MCF-7 cells with eEF2 knockdown decreased significantly(t = 9. 244 and 5. 938,P = 0. 001and 0. 004,respectively). The expression levels of eEF2 protein in sh1,sh2 and sh3 groups decreased significantly(t =3. 552,9. 614 and 4. 432,P = 0. 024,0. 001 and 0. 011,respectively).Conclusion The lentivirus vector with low expression of eEF2 gene and the stably transfected breast cancer cell model were successfully constructed,which is expected to provide a new target for clinical treatment of breast cancer based on translation-targeted therapy.

The study aims to identify related substances in orlistat pharmaceutical substance from different production technologies. Advanced technologies including UPLC-QTOF-MS and UPLC-QTrap-MS were used and the structures or molecules of 10 kinds of related substances in orlistat were identified. The experimental data were valuable to the quality control and development of orlistat.

OBJECTIVE To investigate the apoptosis of hepatocytes in arsenic poisoning rats caused by coal-burning and explore the effects of p53-induced mitochondrial apoptotic pathway on arsenic liver injury.METHODS Wistar rats were fed with 164.74 pp m arsenic conta minated grain at the levels of 15%,30% and 60% (arsenic contents were 25,50 and 100 mg·kg -1 ,respectively)for 90 d. The arsenic contents of urine and hair,apoptosis of hepatocytes and mRNA expression of p53,Bax and Bcl2 in peripheral blood and hepatocytes were evaluated.At the sa me ti me,protein expression of p53, Bax and Bcl2 in hepatocytes were analyzed.RESULTS The arsenic contents of urine and hair increased with the elevation of arsenic dose.The apoptotic rate of hepatocytes in arsenic 50 and 100 mg·kg -1 group were 16.49 ±2.06 and 15.83 ±1 .28,respectively which were significantly higher than the control group and arsenic 25 mg·kg -1 group (9.00 ±0.59 and 9.27 ±0.36,respectively,P <0.05).p53 mRNA expression of peripheral blood in arsenic 100 mg·kg -1 group was 2.69 ±1 .84 while p53 mRNA expression of hepatocytes in arsenic 25,50 and 100 mg·kg -1 group were 1 .63 ±0.28, 1 .91 ±0.38 and 1 .71 ±0.18,respectively which were significantly higher than the control group (0.86 ± 0.15 and 1 .22 ±0.12,respectively,P<0.05).Bax mRNA expression of peripheral blood in arsenic 50 and 100 mg·kg -1 group were 1 .36 ±0.30 and 1 .94 ±0.65 while Bax mRNA expression of hepatocytes in arsenic 100 mg·kg -1 group was 1 .34 ±0.23 which were significantly higher than the control group (0.77 ±0.15 and 0.84 ±0.34,respectively,P<0.05).Bcl2 mRNA expression of hepatocytes in arse-nic 100 mg·kg -1 group was 0.98 ±0.50 which was significantly lower than the control group (2.14 ± 1 .15,P<0.05).p53 protein expression of hepatocytes in arsenic 25,50 and 100 mg·kg -1 group were 1 .06 ±0.56,1 .15 ±0.77 and 0.74 ±0.27,respectively while Bax protein expression of hepatocytes in arsenic 50 and 100 mg·kg -1 group were 0.74 ±0.43 and 0.69 ±0.37 which were significantly higher than the control group (0.36 ±0.1 9 and 0.25 ±0.09,respectively,P<0.05).CONCLUSION Arsenic can induce hepatocytes apoptosis and p53-mediated mitochondrial apoptotic pathway may be involved in the develop ment of rat liver injury in arsenic poisoning rats caused by coal-burning.

Chloasma is a kind of skin disease with pigmentation on the face, which can be frequently seen in child-bearing age women. The disease is caused by Zangfu dysfunction, Qi and blood deficiency. For women, the generation of chloasma is closely related to their menstruation. Regulating the menstrual function with traditional Chinese medicine and balancing the body's internal environment can be very useful for achieving the balance between Yin and Yang within Zangfu, and regulating Qi and blood, which will not only desalt pigment, but also can improve the symptom related to menstruation with low recurrence rate after drug withdrawal. This method can be used alone or with other methods in clinic, has achieved significant effect.

Objective To investigate the influence of different excipients on the stability of baicalin solution.Methods The thermostatic accelerated test was used to observe the effect of solubilizing agent (Meglumine and Glucose), solubilizer (Pluronic F- 68) and stabilizer (HP- ? - CD) on baicalin stability and the concentration of baicalin was determined by HPLC. Results The valid period of baicalin solution with solubilizing agent added was shortened, and that with solubilizer and stabilizer added prolonged. Conclusion It is feasible to increase the stability of baicalin solution with Pluronic F- 68 or HP- ? - CD added. 

Hyperglycemia is considered to be a predictor of death, poor functional outcome, symptomatic intracerebral hemorrhage, and increased infarct volume in patients with acute ischemic stroke. Many studies have used parameters such as standard deviation, coefficient of variation, blood glucose level, stress hyperglycemia ratio, and glucose/glycated hemoglobin ratio to express hyperglycemia, and then study the relationship between hyperglycemia and stroke outcome. In patients with stroke receiving intravenous thrombolysis, hyperglycemia is closely associated with symptomatic intracerebral hemorrhage, poor neurological outcome, and lower vascular recanalization rate. In patients with stroke undergoing endovascular mechanical thrombectomy, hyperglycemia is closely associated with poor long-term outcome, increased mortality, and increased infarct volume. However, whether hyperglycemia will affect the effect of reperfusion therapy in acute ischemic stroke remains controversial. The possible mechanisms of hyperglycemia leading to poor outcomes after reperfusion therapy in patients with ischemic stroke include inhibition of vasodilation, reduction of plasma fibrinolytic activity, destruction of the blood-brain barrier, promotion of oxidative stress, and reduction of ischemic penumbra volume. Studies have shown that for stroke patients with hyperglycemia, intensive blood glucose control does not bring significant benefits. Therefore, the blood glucose control program of patients with acute ischemic stroke and hyperglycemia needs further study and discussion.

Objective To evaluate the effect of interactive case teaching(ICT) method in teaching hematology.Methods Sixty students were randomly selected from 2009 grade clinical medicine majors in the Third Military Medical University and were divided into experimental group and control group,each group with 30 students.Students in experimental group used ICT； while those in control group used traditional teaching method.Closed book exam was conducted for students in experimental group and control group at the end of the semester； question types of the exam included concept questions (nouns explain,choice,short answer questions) for 70 points,cases analysis question for 30 points,a total of 100 points.Data were expressed as mean ± standard deviation ((x) ±s) and analyzed with SPSS 20.0 independent sample t test.Questionnaire was designed and feedback from students was statistically analyzed.Results There was no statistical differences between experimental group and control group in concept questions (P =0.580),however,in terms of cases analysis question,scores in experimental group were significantly higher than those in control group (P =0.000) ； total scores of experimental group were also significantly higher than those in control group (P =0.016).Feedback showed that the students highly valued the form and effect of ICT.Conclusions Using ICT in teaching hematology can arouse students' enthusiasm for study,cultivate their independent thinking and ability of problem-solving.Meanwhile,ICT can combined theoretical teaching with clinical practice well and improve the quality of teaching.

Colorectal cancer is one of the common malignant tumors and the overall prognosis is poor. The search for a more effective treatment for colorectal cancer has never stopped. The current interaction between the modulated immune system and the tumor microenvironment is a hot topic in the treatment of colorectal cancer. The achievements involve immune checkpoint inhibition, cytokine therapy, toll?like receptors and autologous cell therapy. It has been proved that these methods have mild effect on tumor loading reduction. However, significant breakthrough has been achieved with the use of checkpoint inhibitors targeting cytotoxic T lymphocyte associated antigen?4 (CTLA?4),programmed death?1 (PD?1) and programmed death receptor ligand 1 (PD?L1). Immunotherapy is promising in the treatment of patients with refractory tumors. The success of this current immunotherapy approach is largely limited to tumors with high mutation amplification, such as melanoma,renal cell carcinoma ( RCC) and non?small cell lung cancer. However,this discovery has led the development of checkpoint inhibitors in the treatment of colorectal cancer with highly mutated amplification of mismatch repair gene to a new era.