Objective To observe and compare the blood lipid level in patients with colorectal polyps.Methods One hundred and twenty patients with colorectal polyps adminated in Shuangshan Hospital of Anshan from January 2011 to January 2014 were selected as observations and 120 healthy check-ups were selected as control group.The levels of serum total cholesterol (TC), triglyceride (TG) and low density lipoprotein cholesterol(LDL-C) of two groups were detected.Based on the pathological type and position of colorectal polyps and patients' gender, the blood lipid levels of sub-groups were compared.Results For observation group,there were single polyps in 81 cases ,multiple polyps in 39 cases and total colorectal polyps in 7 cases.There were 53 cases (44.17％) of hyperlipidemia (HLP).The levels of TC, TG and LDL-C of observation group were (5.26±0.97) mmol/L, (1.55±0.59) mmol/L, (2.88±0.67) mmol/L, significantly higher than those of control group ((4.51±1.02) mmol/L, (1.05±0.57) mmol/L, (2.43±0.39) mmol/L;t =4.08,4.27, 3.87, P＜0.05).the incidence of HLP based on the different pathological types were not significantly changed (P ＞ 0.05).The blood lipid levels based on the different pathological types were not significantly changed(P＞0.05).According to analysis results of adenomatous polyp, the incidence of tubular and villous adenoma HLP was significantly higher than that of tubular and villous adenoma(x2 =4.51, P＜0.05), while there was no significant difference in term of the blood lipid levels between the two groups(P＞0.05).The specific blood lipid levels were different (95％ CI 1.695-5.276, 1.823-3.517, 1.634-4.186;P ＜0.05).Conclusion TC and TG increase in patients with colorectal polyps.The clinical diagnosis and treatment shall focus on the detection of blood lipid levels.

Dietary intake and biochemical parameters of zinc nutritional status in 270 pregnant women and 44 nonpregnant women have been studied and the results were as follows: 1.Dietary zinc intake was about 60% of RDA in both groups.2.The mean plasma Zn concentration declined progressively during gestation; the mean SOD activities and plasma Cu/Zn and alkaline phosphatase activities increased progressively.3.The zinc nutritional status of pregnant women in Beijing was poor. Using plasma Zn concentration less than 10.71 ?mol/l as a low limit, the incidence of zinc deficiency was 6.5%, 20.2% and 25.7% at each of three trimesters respectively.4.Plasma Zn concentration is more sensitive in evalution of nutritional status than hair Zn concentration. Plasma Cu/Zn could reflect zinc nutritional status. The mean SOD activities had similar trend with plasma Zn during pregnancy, and positive correlation with plasma Zn in second trimester (r= 0.2236,P

It is the trend to integrate the curriculum contents and strengthen the ability training in reform of edu-cation model at present .After the abilities to develop and maintain medical informatics-related information sys-tems, to process medical information and to provide medical information service were analyzed, certain suggestions were put forward for the practical teaching reform of medical informatics according to the requirements of ability training and the characteristics of subject knowledge structure .

Objective To search for the CT and MRI morphological changes in the child patients with cerebral palsy. Method The CT scranning and MRI were carried out in 150 child patients with cerebral palsy, andthe results were analysed. Results In 150 cases, 97 cases (54.6 per cent) have abnormal findings of CT scranning. Diffuse ence-phalatrophy (42 cases), encephalomalacia focus (24 cases) porencephaly (9 cases), and localized ence-phalatrophy (7 cases) ect are the common CT morphological changes in the patients with cerebral palsy.In the 53 patients with normal findings of CT scranning, MRI was carried out for 30 cases and 25 cases(83.3 per cent) were abnormal. The delayed myelinogeny of white matter of brain is the main MRI mor-phalogical abnormality. Conclusion CT scranning and MRI have important value on the etiological diagnosis for child patientswith cerebral palsy. The combination of them can improve the diagnostic accordance rate.

OBJECTIVETo investigate the correlation between drinking behavior and polymorphism combination of extracellular superoxide dismutase (EC-SOD) and aldehyde dehydrogenase 2 (ALDH2) genes and oral squamous cell carcinoma.

METHODSThe genetic polymorphisms of EC-SOD and ALDH2 were analyzed by polymorphism-polymerase chain reaction technique in peripheral blood leukocytes of 750 oral squamous cell carcinoma cases and 750 non-cancer controls.

RESULTSThe frequencies of EC-SOD (C/G) and ALDH2 variant genotypes were 38.27% and 69.47% in oral squamous cell carcinoma cases and 21.07% and 44.40% in healthy controls, respectively. Statistical tests showed significant difference in the frequencies between the two groups (P < 0.01). The risk of oral squamous cell carcinoma with EC-SOD (C/G) was significantly higher than that of controls (OR = 2.32). Individuals carrying ALDH2 variant genotypes had high risk of oral squamous cell carcinoma (OR = 2.85). Combined analysis of the polymorphisms showed that percentages of EC-SOD (C/G)/ALDH2 variant genotypes in oral squamous cell carcinoma and control groups were 30.67% and 6.80%, respectively (P < 0.01). Individuals carrying EC-SOD (C/G)/ALDH2 variant genotypes had high risk of oral squamous cell carcinoma (OR = 8.13). The drinking rate of the case group was significantly higher than that in the control group (OR = 2.70). Statistical analysis suggested an interaction between drinking and EC-SOD (C/G) and ALDH2 variant genotypes, which increase risk of oral squamous cell carcinoma (OR = 25.00).

CONCLUSIONEC-SOD (C/G) and ALDH2 variant genotypes and drinking are the risk factors in oral squamous cell carcinoma, which could carry out a coordinated attack of oral squamous cell carcinoma.

Aldehyde Dehydrogenase ; Carcinoma, Squamous Cell ; Drinking ; Genotype ; Humans ; Male ; Middle Aged ; Mouth Neoplasms ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Risk Factors ; Superoxide Dismutase

Objective:To obtain monoclonal antibodies(McAb) against Staphylococcal enterotoxin C2(SEC2) and establish method for detecting SEC2.Methods:The secreted SEC2 from staphylococcus aureus was used as antigen to immune BALB/c mice. Monoclonal antibodies against SEC2 were prepared by normal hybridoma technique. By identifying the characters of McAbs, the quantitative detection ELISA test method were established and were preliminarily applied.Results:Four hybridmas producing antibodies against SEC2 were obtained. IgG isotypes of four McAbs were IgG1. Their binding site was different except two McAbs that shared the same binding site.The McAbs were proved to be specific for SEC2.The sandwich ELISA method had good specificity, sensitivity and reproducibility, and it was founded to be able to detect SEC2 at concentration from 0.5 to 20 ng/ml. Its recovery ranged between 97.8% and 101%,and CV value ranged between 2% and 5%.Conclusion:The prepared McAb against SEC2 can be used for SEC2 immunoassay. This work provides a basis for controlling the quality of JINPUSU and researching staphylococcal enterotoxin.

Objective This study was designed to investigate the relationship between programmed cell death 5 (PDCD5) and endometrial carcinoma .Methods 60 tissue samples of endometrial carcinomas ,40 normal proliferative endometrium tissues and 28 endometrial atypical hyperplasia tissues were obtained from patients who underwent operation.The expression of PDCD5 was detected by Real-time PCR and immunohistochemical analysis . Results In normal proliferative endometrium , atypical hyperplasia tissues and endometrial carcinoma , the PDCD5 mRNA was gradually decreased[(1.21 ±0.16),(0.83 ±0.07),(0.42 ±0.02),F=5.637,P<0.05];The posi-tive rate of protein was gradually decreased (82.5%,64.3%,45.0%,χ2 =27.663,P<0.05);The expression of PDCD5 was correlated with TNM stage , histological grade , depth of myometrial invasion and lymphatic metastasis (χ2 =20.875,11.915,9.409,11.148,all P<0.05),but was not correlated with ages (χ2 =0.287,P>0.05). Conclusion PDCD5 might become a potential molecular marker of early diagnosis of endometrial carcinoma .

A monoclonal antibody (McAb) 3H11 against gastric cancer with high positive rate, high selectivity and high affinity was obtained. 131 I Labeled McAb 3H11 was injected into gastric cancer-bearing nude mice, and the tumor/liver ratio, localization index, ID%/g could reach 8.26?1.26, 6.08 + 1.51, 11.00?2.62, respectively. Moreover, McAb 3H11 conjugated with 131 I could increase the cytotoxicity to tumor xenografts and decrease the side effects. The radioimmunoimage was carried out in 19 cases with gastric cancer, and positive image was obtained in 16 cases (84.2%). The radioimmunoguided surgery of gastric cancer was successfully done by submucous injection of labeled McAb 3H11 through endoscope. The sensitivity, specificity and accuracy for evaluation of tumor infiltrated gastric walls were 94.6%, 96.7% and 95.9%, respectively. The sensitivity, specificity and accuracy for identification of metastatic lymph nodes were 99.2% ,97.7% and 98.8%, respectively.

Objective In this study,the effects of moderate treadmill exercise and estrogen replacement treatment on the production of liver nitric oxide(NO)and the expression of endothelial nitrie oxide synthase(eNOS)and inducible nitric oxide synthase(iNOS)in ovariectomized(OVX)rats were observed.Methods Female mature SD rats were randomly divided into six groups according to their body weight:sham group(group S),sham exercise group (group S+EX),group OVX,OVX+exercise group (group OVX+EX),OVX+estrogen group(group OVX+E_2),and OVX+exercise+E_2 group(group OVX+EX+E_2).The rats ran on the treadmill at a speed of 18m/min for 60min,5 times per week.50μg/kg of E_2 was given subcutaneously three times per week to groups OVX+E_2 and OVX+EX+E_2.After 14 weeks,NO production in the liver was determined by nitrate reductase method.Protein expression of eNOS and iNOS was determined by immunohistochemistry.Results The NO content of group S was significantly lower than that of group S+EX,but significantly higher than that of group OVX.The NO content of groups OVX+EX,E_2,and OVX+EX+E_2 were significantly higher than that of group OVX.The positive reaction of eNOS mainly located in the cytoplasm of hepatocytes and endothelial cells of the hepatic sinusoids in all groups.The changing tendency after exercise or/and estrogen replacement was almost similar with that of the content of NO.The positive reaction of iNOS mainly located in the cytoplasm of hepatocytes in groups S,S+EX,E_2,and OVX+EX+E_2,while it located in the nuclei in both groups OVX and OVX+EX.Conclusion Moderate exercise and estrogen supplementation both had the same effect of upregnlated synthesis of NO in liver of ovariectomized rats.

Objective To explore the clinical effect of moist dressing combined with high energy narrow band phototherapy on chronic dermal ulcer.Methods Sixty cases with dermal chronic ulcer were randomly divided into two groups equally.The experimental group was treated by moist dressing combined with high energy narrow band phototherapy,while the control group was treated by moist dressing.The areas of wound surface were compared after treating for 14 and 21 days.Result Twenty-one days after treatment,the areas of wound surface were significantly reduced in the experimental group than that in the control group(P<0.05).Conclusion Moist dressing combined with high energy narrow band phototherapy can eradicate necrotic tissue,adjust micro-environment,and promote granulation and healing in chronic dermal ulcer.