Objective To explore the change of ECG after chemotherapy and radiotherapy in treatment of patients with breast cancer. Methods 58 patients with breast cancer were treated with surgery,after operation the observation group were treated with chemotherapy and radiotherapy,and the control group was given chemotherapy singly.The change of ECG was compared between two groups. Results In the observation group,11 patients had abnormal ECG(37.93%),In the control group,2 patients had abnormal ECG(9.52%)(x2=8.03,P ＜0.05＝.In addition,in the observation group the incidence of abnormal ECG in the patients with left breast cancer(7/15)was slightly higher than that with right breast cancer(4/14)(x2=1.01,P ＞ 0.05). Conclusion The treatment of chemotherapy combined with radiation could significantly increase cardiac toxicity in the patients with breast cancer.

Most of the patients who suffer from secondary iron overloading are transfusion-dependent.Excess iron deposits in the important organs will have notable effect on survival.The serum ferritin can not reflect the iron stores in the specific organ rigorously.There are two noninvasive techniques that can assess tissue iron:superconducting quantum interface device and MRI-T2*.The patients who are transfusion-dependent should monitor the body iron stores and chelate the iron in time.Now the drugs for iron chelation therapy include deferoxamine,deferiprone and deferasirox.

Objective To observe the effect of injection needle scalpel combined with Tongbi tincture heat deposited on the erythrocyte sedimentation rate, CRP and IL-1 in knee osteoarthritis.Methods 96 cases of knee osteoarthrosis according to the treatment divided into the control group(n=48) and experimental group(n=48), the control group was treated with injection needle scalpel, the experimental group on the basis of injection needle scalpel combination of tongbi tincture heat deposited, all lasted for 14 days.The clinical efficacy, CRP, IL-1, blood sink, knee joint functional impairment score, and safety was observed in the two groups.Results The total effective rate of the experimental group was 93.75%,higher than the control group 79.17%(P<0.05).After treatment, the CRP, IL-1, blood sedimentation of experimental group was lower than the control group[(7.09±0.97)mg/L,(6.54±0.82) ng/L,(16.47±2.03) mm/h vs.(10.35±1.28) mg/L,(7.21±0.93) ng/L,(18.25±2.21) mm/h](P<0.05).The scores of knee joint function impairment in the experimental group was higher than that in the control group(P<0.05).There was no significant difference in safety between the two groups.Conclusion The therapeutic effect of injection needle scalpel combined with Tongbi tincture heat deposited in treatment knee osteoarthrosis is better than injection needle scalpel.It can promote the absorption of inflammation, relieve knee function.

OBJECTIVE:To investigate mycoplasma infection and drug susceptibility in patients with non-gonococcus urethritis (NGU),and to provide reference for rational use of antibotics in patients with mycoplasma infection.METHODS:Mycoplasma diagnosis reagent box was used to culture and identify mycoplasma from suspected patients with NGU,and to conduct drug sensitivity test.RESULTS:360 cases of positive mycoplasma among 711 suspected NGU patients (positive rate was 50.66%),Uu positive were 235 cases (positive rate 33.05%);Mh positive were 30 cases (positive rate 4.22%);Uu+Mh positive were 95 cases (positive rate 13.36%).Results of drug sensitivity test showed josamycin,doxycycline,minocycline,clarithromycin and tetracycline were best choice for Uu single infection,and josamycin,doxycycline,minocycline for Uu+Mh infection.CONCLUSION:Uregenital tract infections are mainly caused by Uu.Clinical medication for mycoplasma infection should be in accordance with the type of mycoplasma and results of drug sensitivity test.

OBJECTIVE: To analyze the utilization of Chinese patent medicines in our hospital to provide scientific basis of rational use of drugs in clinic.METHODS: Data about annual consumption sum and consumption amount of Chinese patent medicines in our hospital during 2005～2009 were collected to analyze annual consumption sum,DDDs end daily cost of Chinese patent medicine statistically.RESULTS: The annual consumption sum of Chinese patent medicines showed a upward trend in our hospital during 2005～2009.The top 15 Chinese patent medicine in the list of consumption sum which took up a large proportion affected the total consumption sum.Injection was used more and more so that the ratio of its consumption sum to rank of DDDs was lower than one.CONCLUSION: Cheap and effective Chinese patent medicines play an important part in the clinic.DDDs order should be emphasized in the drug supervision and Chinese traditional medicines injection should be managed strictly.

OBJECTIVE: To facilitate separate-store management and cold-chain management for hospital drugs. METHODS: The temperature division of separate-store management of hospital drugs was analyzed. The cold-chain management of cold-storage and freeze-storage drugs was discussed. RESULTS & CONCLUSIONS: During the storage period of hospital drugs,the temperature of cold-storeroom should be kept at 2～8 ℃,that of shady and cool storeroom at 0～20 ℃,and that of the normal temperature storeroom at 0～30 ℃. In the cold-chain management,great importance should be attached to several key steps such as to standardize the transport vehicles,set up stores for the turnover of drugs,and to clarify the operational procedure and working standard. It is necessary to strengthen separate-storehouse management and cold-chain management for hospital drugs.

Objective To evaluate the optimized efficacy of thoracic paravertebral block (TPVB) combined with general anesthesia via the research on the postoperative cellular immune function in the patients undergoing radical resection for esophageal cancer.Methods Forty American Society of Anesthesiologists physical status Ⅰ or Ⅱ patients of both sexes,aged 45-64 yr,with body mass index of 20-25 kg/m2,scheduled for elective radical resection for esophageal cancer,were divided into 2 groups (n =20 each) using a random number table:general anesthesia group (G group) and TPVB combined with general anesthesia group (TPVB+G group).In group TPVB+G,TPVB was performed at T4 and T6 on the operated side under ultrasound guidance with 0.5％ ropivacaine 15 ml before anesthesia induction.Anesthesia induction was started after the level of block was confirmed.Anesthesia was maintained with propofol given by target-controlled infusion at the target plasma concentration of 1-4 μg/ml,and bispectral index value was maintained at 40-50.Patient-controlled intravenous analgesia was performed after operation,and visual analog scale score was maintained ≤ 3.Before anesthesia induction,at the end of operation and at 24 and 48 h after operation,blood samples were collected from the central vein for determination of the levels of T lymphocyte subsets CD3+,CD4+ and CD8+ and CD4+/CD8+ ratio.Ricker Sedation-Agitation Scale scores were recorded at 10 min,20 min,30 min and 1 h after extubation and 6,24 and 48 h after operation.The consumption of intraoperative remifentanil,requirement for vasoactive agents during recovery from anesthesia,the number of unsuccessfully delivered doses and the total number of attempts were also recorded.Results Compared with group G,Ricker Sedation-Agitation Scale scores were significantly decreased at 10 min after extubation,the consumption of intraoperative remifentanil,requirement for vasoactive agents during recovery from anesthesia and the number of unsuccessfully delivered doses were decreased,and the postoperative CD4+ level and CD4 +/CD8 + ratio were increased in group TPVB+G (P＜0.05).Conclusion TPVB combined with general anesthesia can improve the postoperative cellular immune function and is an optimal anesthesia regimen in the patients undergoing radical resection for esophageal cancer.

Objective: To investigate the trends in incidence and mortality of stroke in Ningbo City, Zhejiang Province from 2012 to 2021, so as to provide the evidence for improving the stroke control strategy in Ningbo City. Methods: Data pertaining to the stroke incidence and mortality in Ningbo City from 2012 to 2021 were captured from Ningbo Municipal Chronic Disease Collaborative Management System, and the incidence and mortality of stroke was estimated and standardized by the Sixth National Population Census in 2010. The trends in incidence and mortality of stroke were evaluated using annual percent change (APC) in Ningbo City from 2012 to 2021. Results: A total of 185 747 residents with stroke were reported in Ningbo City from 2012 to 2021. The crude incidence of stroke was 312.78/105 in Ningbo City from 2012 to 2021, which appeared a tendency towards a rise (APC=2.429%, t=5.507, P<0.001), and the standardized incidence of stroke was 188.86/105, with no remarkable changes seen (APC=-0.399%, t=-1.544, P=0.161). There were 47 355 deaths due to stroke in Ningbo City from 2012 to 2021, and the crude and standardized mortality rates were 79.74/105 and 42.79/105, which both appeared a tendency towards a decline (APC=-4.778%, t=-6.867, P<0.001; APC=-8.269%, t=-13.729, P<0.001). Higher standardized incidence and mortality were seen among men (226.05/105 and 50.14/105) than among women (153.37/105 and 35.89/105), and the crude incidence and mortality of stroke appeared a tendency towards a rise with age (χ2trend=3 671.325, 1 419.107, both P<0.001). The standardized incidence and mortality of hemorrhagic stroke were 40.38/105 and 18.56/105, both showing a tendency towards a decline (APC=-5.042%, t=-12.958, P<0.001; APC=-11.570%, t=-14.979, P<0.001), and the standardized incidence of ischemic stroke was 143.91/105, appearing a tendency towards a rise (APC=1.254%, t=3.223, P=0.012), while the standardized mortality of ischemic stroke was 19.65/105, with no obvious changes seen (APC=-2.110%, t=-2.150, P=0.064). Conclusions The incidence of stroke appeared a tendency towards a rise and the mortality appeared a tendency towards a decline in Ningbo City from 2012 to 2021. Men and the elderly are at high risk of stroke.

OBJECTIVE:To provide reference for the improvement of Metaanalysis. METHODS:13 repots about Meta-analysis of randomized controlled trials(RCTs)on antivirus traditional Chinese medicine(TCM)injections for respiratory tract infections were collected and analyzed. Their qualities were evaluated using QUOROM statement,CONSORT statement,Jadad scale and the quality evaluation method for Metaanalysis posed by Sacks,etc. Disagreements were resolved by consensus. RESULTS:The obtained average mark of 13 studies was(64?10.13)with the highest mark of 84 and the lowest of 52. Quality evaluations of reports were conducted from 5 aspects including 25 items. 5 aspects were all considered in 13 studies. 60% of the 25 items were in- volved in 13 reports at least and 88% at most. CONCLUSION:Meta- analysis methods are improved significantly in China. It should be still standardized to make sure correctness and reliability of results of meta-analysis. Meta-analysis method should be stan-dardized to obtain correct and reliable results.

A sensitive method based on high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed for the simultaneous determination of folic acid (FA) and its active metabolite, 5-methyltetrahydrofolic acid (5-M-THF), in human plasma. The analytes were extracted from plasma with methanol solution containing 10 mg/mL of 2-mercaptoethanol and 0.025% (v/v) ammonium hydroxide. FA and 5-M-THF were more stable after the addition of 2-mercaptoethanol and ammonium hydroxide in the sample preparation procedures of this study than they were in the previously published methods. Chromatographic separation was performed on a Hedera ODS-2 column using a gradient elution system of acetonitrile and 1 mM ammonium acetate buffer solution containing 0.6% formic acid as mobile phase. LC-MS/MS was carried out with an ESI ion-source and operated in the multiple reaction monitoring (MRM) mode. The assay was linear over the concentration ranges of 0.249-19.9 ng/mL for FA, and 5.05-50.5 ng/mL for 5-M-THF. The developed LC-MS/MS method offers increased sensitivity for quantification of FA and 5-M-THF in human plasma and was applicable to a pharmacokinetic study of FA and 5-M-THF.