Objective:To investigate the regulatory effect of cimetidine on proliferation and apoptosis of colon cancer cells LoVo in vitro.Methods:Colon cancer cell line LoVo originated from human colon carcinoma was cultured in vitro.Cell proliferation was measured by MTT colorimetric assay.Cell cycle and apoptotic percentage were detected by flow cytometry (FCM),morphological changes of apoptotic cell were observed by light and electron microscopy,and apoptotic cells were quantitatively determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL).In addition,expression of intracellular bcl-2 protein was analysed by flow cytometric indirect immunofluorescent technique.Results:Cimetidine (1?10 -2 mol/L～3?10 -2 mol/L) inhibited LoVo cells growth significantly in a dose-dependent manner.After 48 hours of exposure to cimetidine(1?10 -2 mol/L),the enhanced degree of apoptotic percentage was 32.7%.There were many morphologic features of apoptosis,including cell shrinkage,nuclear condensation,DNA fragmentation and formation of apoptotic bodies;apoptotic cells were also statistically significant than those of control group( P

BACKGROUND:In recent years, delayed protection of drug pretreatment has become a hot spot in the field of pretreatment, and sufentanil is a non-selective opioid receptor agonist that plays a significant myocardial protective role.
OBJECTIVE:To investigate the protective effect of sufentanil combined with umbilical cord blood stem cel transplantation on injured myocardium after myocardial infarction.
METHODS:Myocardial infarction model by ligating the left anterior descending coronary artery (ischemia 30 minutes and reperfusion 180 minutes) was made in 90 healthy adult Wistar rats, and then these rat models were randomly divided into ischemia-reperfusion (IR) group, cel transplantation group, combined treatment group (n=30/group). Rats in the IR group were given intraperitoneal y intravenous injection of 1 mL normal saline 5 minutes before IR;rats in the cel transplantation group were given intraperitoneal y intravenous injection of 1 mL umbilical cord blood stem cel suspension 5 minutes prior to IR;and rats in the combined treatment group were given intraperitoneal y intravenous injection of 10μg/kg sufentanil 10 minutes before IR and 1 mL umbilical cord blood stem cel suspension 5 minutes prior to IR. Two weeks later, related indicators were detected.
RESULTS AND CONCLUSION:Compared with the IR group, myocardial infarct size was reduced significantly in the combined treatment group, fol owed by the cel transplantation group (P<0.05). Lowest levels of serum creatine kinase, lactate dehydrogenase and troponin I were obtained in the combined treatment group fol owed by the cel transplantation group and IR group, while highest level of nitric oxide was observed in the combined treatment group fol owed by the cel transplantation group and IR group (P<0.05). Highest expression of Caspase-3 protein was recorded in the combined treatment group, fol owed by the cel transplantation group and IR group (P<0.05). Significantly increased left ventricular diastolic blood pressure and decreased left ventricular end diastolic pressure were found in the two cel transplantation groups compared with the IR group (P<0.05). Moreover, pathological injury of the myocardial tissues was mitigated in the cel transplantation group and significantly relived in the combined treatment group compared with the IR group. In conclusion, the combined use of sufentanil and umbilical cord blood mesenchymal stem cel transplantation can reduce the degree of myocardial infarction and protect against myocardial injury.

Objective To explore the relationship between pituitary tumor transforming gene(PTIG) and Ki67 expression and their significance in multiple adolescent breast fibroadenomas (fibroadenomatosis)and single persons(fibroadenomas) and their clinical value.Methods A total of 53 fibroadenomatosis and 160 fibroadenomas specimens in our hospital were collected.All the samples were fixed with 10％ formalin and then embedded with paraffin.The protein expression of PTTG and Ki67 was detected by immunohistochemical staining.The relation between expression of PTTG and Ki67 in fibroadenomatosis was analyzed.Results The positive rate of PTTG and Ki67 was higher in fibroadenomatosis than in fibroadenomas (P ＜ 0.005).Conclusions High expression of PTTG and Ki67 in fibroadenomatosis leads to the proliferation of tumor cell and upregulates tumor angiogenesis.High expression of PTTG and Ki67 may play important roles in tumorigenesis and pathological process of fibroadenomatosis.

Objective:To investigate the airborne pollen concentration in Beijing city during autumn and to grade the Artemisia pollen concentration .Method:Burkard volumetric trap was used to sample the airborne pollen in Beijing city from August 1st 2007 to October 10th 2007. Patients only allergic to mugwort pollen were followed up, and their symptom scores and the mugwort pollen concentration were analyzed statistically.Result:①Mugwort and Humulus(including Cannabis Sativa L) were the main airborne pollen during August and September in Beijing city, accounting for 31% and 51%,respectively; ②Mugwort pollen season was from 8th August to 8th October;③The daily peak concentration of Mugwort pollen was 267 g/m~3,with an average of 71 g/m~3,the daily peak concentration of Humulus pollen was 672 g/m~3,with an average of 124 g/m3;④88.5% out-patients suffering from hay fever or asthma during Autumn were allergic to mugwort, 28.2% allergic to Humulus;⑤the grading of airborne Mugwort pollen concentration: 0～14 g/m~3 as grade 0, 15～40 g/m~3 as grade Ⅰ, 41～116 g/m~3 as grade Ⅱ,more than 117 g/m~3 as grade Ⅲ.Conclusion:It was the first time that Burkard volumetric sampler was employed in monitoring Mugwort and Humulus concentration in Beijing city. Average concentration of mugwort pollen was about 100 g/m~3, and concentration grade Ⅲ of mugwort pollen was distributed during the last ten days of August and the beginning of September.

Objective To investigate ICU nurses' perceptions towards incontinence-associated dermatitis.Methods Totally 300 ICU nurses in Suzhou were investigated with a self-designed questionnaire.Results College education was considered to be their main source of knowledge about incontinence-associated dermatitis in 53.2 percent of the nurses.99.8 percent of nurses thought college education was far enough for clinical practice.68.5 percent of nurses thought it was very important to train for knowledge of incontinence-associated dermatitis.Conclusions ICU nurses in Suzhou have less knowledge on incontinence associated dermatitis.It can not meet clinical needs.It is recommended that more incontinence-associated dermatitis knowledge should be added in the school education and continuing education in order to improve nurses' professional competency and to reduce the incidence rate of incontinence-associated dermatitis

BACKGROUND: Fetal microchimerism is a kind of stem cells which comes from fetus in pluripara. The research on fetal microchimerism is a hotspot because it may affect the health condition of pluripara.OBJECTIVE: To introduce the methods of how to detect fetal microchimerism, and the function of fetal microchimerism during disease and tissue repair of the mother.METHODS: Fetal microchimerism was used as a key word, and papers published before 2011-05-20 were retrieved in PubMed. Repetitive papers were eliminated.RESULTS AND CONCLUSION: Totally 655 papers were retrieved, and finally 26 were reviewed. There are several methods used for fetal microchimerism detecting, and fetal microchimerism may pay an important role in the pathomechanism of pluripara autoimmune diseases, cancer and tissue repair.

Objective The aim of this study was to understand the pollen concentration and the daily variation during 2007 in Beijing by monitoring.Methods Burkard volumetric strap was used for the pollen sampling from August 1st to October 20th.Genus of pollen was identified by light microscope.Results Artemisia and Humulus.L pollen were the dominant airborne pollen in late summer and autumn in Beijing City.The average pollen concentration of Artemisia and Humulus.L were 71 grain/m3, 124 grain/m3.Both of them lasted from the August 8th until October 6th or 8th.Statistical analysis showed that the pollen concentration of Artemisia or Humulus.L at 2:00 pm to 4:00 pm was higher than that at 6:00 am to 8:00 am.Conclusion It is the first work to conduct the monitoring of the concentration of Artemisia and Humulus.L pollen with Burkard sampler in Beijing.Humulus.L is the dominant airborne pollen during August and September.

Unit discharges of neurons of the hypothalamic ventromedial nucleus region (HVM) and its surrounding structures including paraventricular nucleus, anterior hypothalamic area,and hypolhalamic dorsomcdial nucleus, were recorded in the ureth-ane-anesthetized and flaxidel-immobilized rats. In HVM,8 of 82 units showed periodical fluctuations in discharge rate, which could be categorized into two types: (1) With alternating active and silent phases 5 (2) with gradually waxing and waning phases. The duration of cach cycle varied from 1 to 5 minutes in different neurons. The periodicity disappeared after intraperitoneal injection of nembutal, while the average discharge rate was raised somewhat. This result suggested that the depressing pha-s of periodical activity of the HVM neuron might probably result from the inhibitory action in certain neuronal circuit, rather than from the intrinsic property of the recorded neurons. The effect of picrotoxin was contradictory. No periodical activity was noted in the 47 recorded neurons outside of the HVM.

Objective: To prepare the processing solution of realgar by bacteria and to explore the change of pharmacodynamic action and toxicity. Methods: The processing solutions of realgar were prepared using the strain thiobacillus ferroxidans, the realgar powder processed by normal method was selected as control. The effects on RPMI8226 cell proliferation of them were investigated by methyl thiazolyl terazolium (MTT),cell cycle distribution was determined by FCM in RPMI8226 cell. The distribution of arsenic concentration in blood and tissues including heart, liver, spleen, lung, and kidney in the samples were determined by Inductively Coupled Plasma-atomic Emission Spectrometry (ICP-AES). Results: Compared with control group, the solubility rate and the induced apoptotic rate increased, and dosage significantly reduced, while arsenic was hardly found in the tissue in processing group by bacteria. Conclusions: The processing group by bacteria may be enhanced medicinal efficacy and inhibited the toxicity in realgar. It’s intending to provide new approaches for scientific processing realgar.

Objective To find the best methods to make the extraction of alternaria alternate. Methods Alternaria alternate was harvested from air and identified, cultured at 26 ℃ for four weeks. Protein was extracted by different methods and its concentration was determined using the Bradford assay, its bioactivity was tested by RAST inhibition, and residual proteins were analyzed by SDS-PAGE.Results The extraction methods like grinder(a), grinder plus ultrasonic(b), ground with liquid nitrogen(c) and liquid nitrogen plus ultrasonic(d) were applied in the extraction procedure. The concentrations of protein extractions were as follows (g/mL): a was 0.44?0.04;b was 0.75?0.03;c was 0.72?0.03 and d; 1.29?0.05. From SDS-PAGE, we found that the most component in the extraction was that obtained by liquid nitrogen plus ultrasonic. From RAST, 50% inhibition was as follows(g/mL): a was 8.5, b was 9.4, c was 7.0,d was 3.7. Conclusion Ground with liquid nitrogen plus disintegration by ultrasonic was the most effective method of extraction.