AIM: To investigate the synergistic effect of resveratrol and 5-fluorouracil on osteosarcoma CD133+ cell subset.METHODS: Human osteosarcoma cell line MG-63 CD133+ cell subset and the corresponding CD133-cell subset were treated with resveratrol and 5-fluorouracil.After treatment, the viability of MG-63 cells was measured by MTT assay.The apoptosis of MG-63 cells was analyzed by flow cytometry.Activation of caspase-9 and caspase-3, the expression of Apaf-1, and the release of cytochrome C were evaluated by Western blot.The interaction between Apaf-1 and pro-caspase-9 was detected by co-immunoprecipitation.RESULTS: The cell death and apoptosis of MG-63 CD133+ cell subset induced by 5-fluorouracil were significantly weaker than those in the corresponding MG-63 CD133-cell subset.However, co-treatment with resveratrol significantly enhanced the effect of 5-fluorouracil on inhibiting the viability of MG-63 CD133+ cell subset.Mechanically, treatment with resveratrol upregulated the expression of Apaf-1.Transfection with Apaf-1 siRNA abolished the synergistic effect of resveratrol and 5-fluorouracil in MG-63 CD133+ cell subset.In addition, the results of co-immunoprecipitation indicated that the combination of resveratrol and 5-fluorouracil significantly induced the formation of Apaf-1/pro-caspase-9 complex, leading to the activation of caspase-9 in MG-63 CD133+ cell subset.CONCLUSION: Resveratrol enhances 5-fluorouracil-induced apoptosis of osteosarcoma CD133+ cell subset by promoting the formation of Apaf-1/caspase-9 complex.

OBJECTIVE:To study the vasodilatation effects of the alcohol extract from Uighur medicine Ziziphora clinopodioi-des(EEZ)on isolated rat thoracic aorta vessel rings. METHODS:Isolated rat thoracic aorta vessel rings were prepared. There was a group with intact vessel ring endothelium and a group with vessel ring endothelium removed in the test. After preshrinking the ves-sel rings with phenylephrine(PE),EEZ of 100,300,500,700,900 and 1 100 mg/L was added gradually,concentration-vasodila-tation curve was drawn and maximal vasodilatation rate (Emax) and median effective concentration (EC50) were calculated. For the group with vessel ring endothelium removed,after the vessel ring was pretreated with EEZ at EC50 in calcium-free solution or calci-um-free high-potassium solution,CaCl2 of 0.4,0.8,1.2,1.6,2.0 and 2.4 mmol/L was added gradually,and calcium concentra-tion-tension curve was drawn;following the pretreatment of the vessel ring with EEZ at EC50,1 μmol/L PE was added and shrink tension was recorded and vasoconstriction percentages was calculated. RESULTS:EEZ had vasodilatation effect on the vessel ring preshrunk with PE in a concentration and smooth muscle-dependent manner. The group with intact vessel ring endothelium and the group with vessel ring endothelium removed respectively had Emax of (58.18 ± 16.23)% and (73.54 ± 17.21)%,and EEZ EC50 of 773.27 mg/L. For calcium-free high-potassium solution,EEZ could cause the calcium ion-vasoconstriction curve to move to the right obviously;for calcium-free solution,EEZ could inhibit the vasoconstriction caused by PE. CONCLUSIONS:EEZ has vasodi-latation effects by a mechanism which may be related to inhibiting calcium influx and intracytoplasmic calcium release through the inhibition of voltage-dependent calcium channels(VDCCs),and thus interfering intracytoplasmic calcium ion balance.

Objective To explore the oxidative damage and genotoxicity of organic chemical pollutants from running water and the source water in Baotou reach of the Yellow River to mice. Methods The Kunming mice were treated with the organic extract solutions of the Yellow River water and running water by gavage at different concentration,once a day and the activity of the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and the content of malondialdehyde (MDA) in the liver and kidney and the rate of micronucleus were determined. Results SOD and GSH-Px activity in the liver and kidney in the groups treated with the water of three sections of Yellow River in Baotou City decreased as the exposure dose increased. Except the low concentration group of running water,the MDA content in the liver and kidney and the micronucleus rate increased significantly compared with the control (P

Objective To study the incidence rate and risk factors of catheter-related infection (CRI) in preemie with peripherally inserted central catheter (PICC),in order to facilitate the implementation of control strategies and control infection.Methods Two hundred and fifty-eight PICC preemie of birth weight less than 2.5 kg,according to whether the incidence of CRI were divided into CRI group and non CRI group,two groups of gestational age,birth weight,PICC time,parenteral nutrition time and PICC parenteral nutrition time were statistically analyzed and compared different indwelling time of CRI incidence.Results Diagnosis of CRI in 24 cases,the occurrence rate of 9.3％ (24/258).There were significant differences in gestational age,birth weight,PICC time,parenteral nutrition time,PICC parenteral nutrition time between CRI group aad non CRI group (29.5 weeks vs.33.0 weeks,1.4 kg vs.1.8 kg,31.5 days vs.14.6 days,40.1 days vs.16.7 days,28.8 days vs.13.4 days,P ＜ 0.01 or ＜ 0.05).There was significant difference in incidence rate of CRI among different indwelling time (P ＜ 0.01).The risk factors of CRI including gestational age (P =0.007),birth weight (P=0.000),PICC time (P=0.001),parenteral nutrition time (P=0.035),PICC parenteral nutrition time (P =0.001).Multivariate Logistic regression analysis showed the greatest impact on the 3 factors were birth weight,parenteral nutrition time,gestational age.Conclusion Gestational age,birth weight,PICC time,parenteral nutrition time are the risk factors of CRI in preemie with PICC.

Ebola virus(EBOV)and Marburg virus(MARV),belonging to the Filoviridae family,emerged four decades ago and caused severe viral hemorrhagic fever in human and other primates.As high as 50-90% mortality,filoviruses can cause significant threats to public health.However,so far no specific and efficient vaccine has been available,nor have other treatment methods proved to be effective.It is of great importance to detect these pathogens specific,rapidly and sensitively in order to control future filovirus outbreaks.Here,recent progresses in the development of detection and diagnosis methods for EBOV and MARV are summarized.

OBJECTIVE:To establish a method for the simultaneous determination of baicalin,baicalein and wogonin in Hua-tan pingchuan tablet. METHODS:HPLC was performed on the column was Diamonsil C18 with mobile phase of methanol-0.1%phosphoric acid(gradient elution)at a flow rate of 1.0 ml/min,detection wavelength was 277 nm,column temperature was 30 ℃, and the injection volume was 10 μl. RESULTS:The linear range was 0.069 04-0.690 4 μg for baicalin(r=0.999 4),0.054 56-0.5456 μg for baicalein(r=0.999 6)and 0.030 36-0.303 6 μg for wogonin(r=0.999 1);RSDs of precision,stability and reproduc-ibility tests were lower than 2%;recoveries were 97.64%-99.06%(RSD=0.57%,n=6),98.31%-100.64%(RSD=0.85%,n=6) and 97.53%-99.48%(RSD=0.76%,n=6). CONCLUSIONS:The method is simple,stable and reproducible,and can be used for the contents determination of baicalin,baicalein and wogonin in Huatan pingchuan tablet.

Human papillomavirus continuous (HPV) infection is an essential factor to induce cervical intraepithelial neoplasia and cervical cancer.Treating HPV infection is considered as a starting point to develop an effective therapeutic vaccine, which is a new strategy for prevention and treatment of cervical cancer.In recent years, development and trials of therapeutic HPV vaccine have made great progress.Selection of vectors, utilization of adjuvants, synthesis of fusion proteins and chimeric proteins have been widely applied to research to enhance vaccine immunogenicity, to increase vaccination safety, to reduce the side effect and so on.The clinical trial results are encouraging: various types of vaccines can induce a specific immune response with good tolerance.However, numerous studies are still required to obtain further success.In addition, HPV exists in various forms, thus it is also the focus of study to expand the range of action and reduce immune escape.

Zishi township of Jiangling city is located in the canal area inside embankment,a heavy endemic area of schistosomiasis japonica in Hubei province. The population and cattle preva-lence was 29.5% and 66.7% respectively in 1992. There are 66 canals and it covered a total area of 300000m2. 97.0% of the canals and 99.3% of the total area had snail inhabitants, and 7.6% of the snails distributed in canal was infected which covered 18.3% of the total area. The snails were mainly distributed in the main canal and their branches. The rate of snail distribution was 45.2% and 39.8% respectively. Cattle was pastured all year round in this area. Examination of scattered cattle feces and water revealed that the major factor of water contamination was scattered cattle feces. It explained why the infection rate of snail was high and the distribution of infected snail was extensive and how the major high rise area inside embankment was formed.

Objective To evaluate the value of furosemide in magnetic resonance urography(MRU) with low field magnet.Methods The routine MRU and MRU after administration of intravein of furosemide were performed with heavily T_2 weighted fast spin echo pulse sequence in 32 cases with urethral diseases.Then three-dimensional(3D) reconstruction for post procedure processing with a maximum-intensity projection(MIP)algorithm was done.Results On routine MRU,there were no image of collecting systems of kidney in 5 cases,and poor images of parenchyma and collecting systems of kidney in 18 cases after using furosemide before MRU,MRU provided high resolution images of parenchyma and collecting systems of kidney in all cases,and images were much clear,the localized diagnosis was accurate.Conclusion MRU using furosemide before scanning can display the parenchyma and collecting systems of kidney distinctively,so furosemide can be regarded as a routine method used before MRU with low field magnet.

Objective: To evaluate the antitumor effect of polysaccharide from spirulina platensis(PSP), and its synergic and attenuated effects on chemotherapeutic drugs in tumor-bearing mice. Methods: Mouse models of transplanting tumor were established. Antitumor effects of PSP and PSP combined with chemotherapeutic drugs were observed and the effect of PSP on leukopenia induced by chemotherapeutic drugs was also investigated. Results: PSP significantly inhibited S180 and H22, the inhibitory rate being 50 %-58 %and 43 %-51 %respectively. PSP evidently increased the antitumor action of cytoxan, PDD and 5-Fu, and elevated the number of white blood cells in leukopenia. Conclusion: PSP can obviously inhibit the tumor growth, also has synergic and attenuated effects on chemotherapeutic drugs.