Objective To study the effects of two disinfection methods for simple respirators in order to select the better sterilization method for the disinfection of simple respirators.Methods One hundred and sixty simple respirators contaminated at the same degree were randomly divided into the manual group and the mechanical group equally .The former took the manual cleaning and disinfection and the latter the mechanical cleaning and disinfection for the disinfection and cleaning of simple respirators.The two groups were compared with regard to the qualified rate of disinfection at the outer and inner surface and the interface of the ball cavity after disinfection cleaning.Results The qualified rates of the mechanical cleaning and disinfection at various parts of the simple respirators were 100%.The qualified rate of manual cleaning and disinfection at the outer surface,the inner surface and the interface were 100%,91.25%and 93.75%,respectively.There was no significant difference in the disinfection effect at the outer surface and interface of simple respirators between the two groups(both P>0.05).But the two kinds of cleaning and disinfections at the inner surface of the respirators were significantly different as regards the qualified rate(P<0.05).Conclusion For the disinfection of simple respirator,the mechanical cleaning and disinfection is better than the manual cleaning and disinfection.

Objective To investigate the effects of mild hypothermic cardiopulmonary bypass(CPB) on bax and bcl-2 protein expression and neuronal apoptosis in hippooampus CA1 region in rats. Methods Healthy male SD rats weighing 380-420g were randomly divided into 3 groups: (1)post-CPB 1h group (n=6); (2) post-CPB 6h group (n=6) and (3) sham CPB group(n=5). The animals were anesthetized with intraperitoneal fentanyl 150?g?kg~(-1), midazolam 1 mg?kg~(-1) and vecurunium 0.1 mg?kg~(-1), intubated and mechanically ventilated. Left femoral artery was cannulated for heparinizafion, BP monitoring and blood sampling. Artery in the tail and right jugular vein were cannulated for CPB. The animals in group 1 and 2 underwent 2 h mild hypothermic (26-28℃) CPB at a flow of 160 ml?kg~(-1)?min~(-1). 1h(group 1) and 6h(group 2) after CPB 4% polymerized formaldehyde was injected via the artery in the tail. Brains were immediately removed. Bax and bcl-2 protein expression in hippocampus CA1 region was determined by immuno-histochemistry method and neuronal apoptosis by TUNEL technique. Ultrastructural changes were examined with electron microscope. Results (1)Bax and Bcl-2 protein expressions were significantly higher in group 1(1h after CPB)than in sham CPB group. Bax protein expression was significantly higher but bcl-2 protein expression was significantly lower in group 2 than in group 1. bax/bcl-2 ratio in group 2(at 6h after CPB)was significantly higher than that in group 1(at 1h after CPB). (2) Neuronal apoptosis was significantly increased in group 1 and 2 compared to sham CPB group. (3) Electron microscopy showed that some mitochondria were moderately or severely swollen with vacuolizafion and decreased number of mitochondrial at 1h after CPB and at 6h after CPB there was shrinkage of neuronal cells with irregular shape of nucleus, notches in nuclear membrane and margination of nucleoli. Conclusion CPB induces upregulation of both bax and bcl-2 protein expression with predominance of the pro-apoptotic gene Bax leading to increased neuronal apoptosis, indicating that CPB can cause brain injury.

Objective To study the role of the cytokine-mediated inflammation in the pathogenesis of acute intestinal mucosa damage and explore the potential mechanisms of glutamine reducing intestinal inflammatory factors on the intcstines and protecting the structure and barrier of intestinal mucosa after traumatic brain iniury(TBI).Methods A total of 54 male Wistar rats were divided equally into nine groups(6 rats in each group),ie,control group,TBI groups(sacrificed at days 1,3,5 and 7 postinjury,respectively)and Glutamine group.Levels of TNF-α and IL-1β in the intestinal tissues were determined by using ELISA and activity of NF-κB by immunohistochemical method.Results Activity of NF-κB in ileum was significantly increased at day 1 following TBI,reached peak at day 3 and remained hjgh level till at day 7 postinjury.TNF-α and IL-1β levels in the intestinal tissues were also significantly increased after TBI,peaked at day 1 and remained high level at day 3 pestinjury.NF-κB activity and the expressions of TNF-α and IL-1β were all obviously decreased posterior to glutamine administration through stomach tube after TBI,compared with normal foodintake group after TBI.Conclusions TBI can induce a rapid and persistent up-regulation of NF-κB and proinflammatory cytokines in the intestine.Inflammation response may play an important role in the pathogenesis of acute intestinal mucosa injury.Glutamine administration throush stomach tube can inhibit NF-κB activity in ileum and reduce TNF-α and IL-1β levels after TBI,indicating that the intestinal barrier function at stress state may be protected by glutamine administration.

Decompression, Surgical ; Humans ; Nose ; surgery ; Ophthalmologic Surgical Procedures ; methods ; Orbit ; surgery

Endoscopy ; methods ; Humans ; Paranasal Sinuses ; surgery

Endoscopy ; Humans ; Nose ; surgery ; Skull Base ; surgery

Humans ; Nose ; anatomy & histology ; surgery ; Petrous Bone ; anatomy & histology ; surgery

To explore the relationship between serum total bile acid concentration and fetal prognosis in patients with intrahepatic cholestasis of pregnancy (ICP).A total of 131 ICP patients were divided into mild and severe ICP groups according to serum total bile acid concentration.And another 100 normal subjects during the same period were selected as control group.Their medical records and fetal prognoses were analyzed.The serum total bile acid,liver enzyme and lipid concentration of ICP patients were all significantly higher than those of the controls (P ＜ 0.01).The fetal prognoses of ICP patients were significantly different with those of controls (P ＜ 0.05).A high serum level of total bile acid of ICP patients may produce serious adverse effects on fetal prognosis.

The early damage detection and evaluation are of great significance in treatment and prognosis to the left ventricular function for children with tumor. In this paper, it is reported that the early damage of the left ventricular function was observed by pulsed wave Doppler (PWD) and tissue Doppler imaging (TDI) in our laboratory. Eighty children half a year to fourteen years old were included in this study. The cardiac function indices in chemotherapy group and control group were measured and compared. The results showed that there was significant difference in mitral and tricuspid annulus flow spectrum between the two groups. Compared with PWD,TDI is more prompt, objective and accurate in detecting early damage of left ventricular function in children with tumor. And TDI is a good method for early identification of ventricular function damage in children with tumor.
Child ; Early Diagnosis ; Echocardiography, Doppler ; Heart ; physiopathology ; Humans ; Neoplasms ; physiopathology ; Ventricular Function, Left

Objective To examine the changes in respiratory mechanics and efficacy of mechanical ventilation during perioperative period in patients undergoing valve replacement and determine the possible causes Methods Twenty ASA Ⅱ Ⅵ patients (8 male, 12 female), aged 24 65 years , undergoing valve replacement were studied The patients were premedicated with intramuscular phenobarbital 0.1g, pethidine 50mg and scopolamine 0 3mg Anesthesia was induced with midazolam, fentanyl and vecuronium and maintained with inhalation of low concentration of desflurane and intermittent intravenous boluses of fentanyl, midazolam and vecuronium Valve replacement was performed with moderate hypothermic cardiopulmonary bypass (CPB), The patients were mechanically ventilated with pure oxygen Tidal volume was maintained at 8 10ml/kg, frequency 12 15 bpm and I∶E ratio 1∶1 5 2 During CPB airway pressure was maintained below 5 cmH 2O After operation the patients were transferred to ICU and mechanically ventilated using Siemens 900C ECG, SpO 2, BP, CVP, left auricle pressure and urine output were routinely monitored Respiratory mechanics was measured with Novametrix 8100 multifunction respiratory monitor Mean airway pressure, peak airway pressure, airway resistance, chest lung compliance and respiratory work were measured 20 min after induction of anesthesia, 10 min before CPB, 10 min after termination of CPB, at the end of operation and 2, 6 and 18h after operation Results Work of breathing and airway resistance increased gradually after induction and reached the maximum at 6h after operation Dynamic compliance decreased significantly before CPB but increased slightly after CPB, then decreased again and to the minimum at 6h after operation Mean airway pressure and peak inspiratory pressure went up significantly after operation All parameters mentioned above returned to the preoperative levels before the patients were weaned from respiratory support in ICU Couclusions Thoracotomy and CPB are the main causes of changes in respiratory mechanics in patients undergoing valve replacement The effects reached the peak at 6 h after operation Therefore mechanical respiratory support is essential for the patients until respiratory function recovers and should be maintained for more than 6h after operation