Periodontal ligament may have independent response to orthodontic stimulation and thus initiate alveolar bone osteogenesis and osteoclasts. Orthodontic-induced alveolar bone remodeling has no bone loss,while inflammation can lead to alveolar bone loss,suggesting that force signal and inflammatory factors may induce the differentiation of undifferentiated cells in the periodontal ligament via different pathways. The strength of the force and the nature of the force (basal tension and fluid shear force) may affect the differentiation of periodontal ligament cells,and may produce antagonistic or synergistic effect with the inflammatory factors through complex autocrine and paracrine regulation,resulting in local bone reconstruction,which is manifested as bone deposition and bone absorption. Studies have shown that Wnt signaling is an important regulatory pathway for osteoblast differentiation. Inflammatory factors can block the differentiation of stem cells into osteoblasts. The Wnt pathway is closely related to the effects of force and inflammatory factors on the differentiation of periodontal ligament cells.

Objective To explore the effect of transcranial magnetic stimulation (TMS) on gross motor function for children with cerebralpalsy. Methods 80 children with cerebral palsy treated in our hospital during July 2009 to January 2010 were divided into control group(n=40) and observation group (n=40). The control group received conventional treatment, including physical therapy, massage, scalp acupuncture,body acupuncture, point injection, physiotherapy and medication. The observation group received TMS in addition. The therapeuticeffects were compared using the Gross Motor Function Measure-88 (GMFM-88) after 3 months. Results The percent scores of all the domainssignificantly increased in both groups (P<0.05) after treatment, the increased percent scores of observation group in B domain, D domain,E domain and total were higher in the observation group than in the control group (P<0.05). Conclusion TMS can further improve thegross motor function of children with cerebral palsy.

Objective: To investigate the expression of pituitary tumor transforming gene (PTTG) and basic fibroblast growth factor (bFGF) in oral squamous cell carcinoma and their relation with each other, as well as the relationship of their expression with clinical-pathological indexes. Methods: The expression of PTTG and bFGF were examined among 55 oral squamous cell carcinoma tissues and 10 normal oral mucosal tissues by the streptavidin-biotinperoxidase (S-P) method. Results:The positive rate of PTTG and bFGF was 78.2% and 67.3% respectively in oral squamous cell carcinoma. The positive rate and grade of PTTG and bFGF were significantly higher than that in the normal mucosa tissues (P

Designed experiment can improve the students'study interest and train their scientific research ability in bioanalytical chemistry experiment teaching.This article probes into the devise of designed experiment,the selectivity of the contents and the detailed process of the experiments.

Monitoring data of antibiotic susceptibility from both home and abroad showed that carbapenem-resistant Enterobacteriaceae (CRE) are being on the rise,which represents an increasingly urgent clinical danger and an emerging public health concern worldwide.In order to effectively prevent CRE transmission in healthcare settings,we should not only pay high attention to the capability building of the laboratory of clinical microbiology,but also actively explore into the mechanisms of carbapenem resistance in Enterobacteriaceae.What's more,we should substantially raise the detection capacity of laboratory on CRE,effectively carry out the antimicrobial stewardship program,and strictly implement hand hygiene and contact precautions.(Chin J Lab Med,2013,36:300-302)

Objective To understand true feelings of patients with malignant tumor after implementing the protective medical treatment.In order to provide more humanized service for clinical nursing and to improve the job satisfaction of nurses.Methods The phenomenological methods of qualitative research was applied to carry out interviews with 12 patients to collected data.Then the data were analyzed.Results After reading,analysis,reflection,classification and refining the theme,we drew the following themes:(1)Before knowing diagnosis:half-believe and anxiety.(2) At the time of knowing diagnosis:luck,avoidance,acceptance and facing; (3) After knowing diagnosis:thirsting for information,low self-worth,improving self motivation.Conclusions We should not insist on hiding malignant tumor patients.Individualized and targeted measures should be taken to make patients cope with diagnosis better and improve the quality of their life.

Ecotropic viral integration site-1 (Evi-1) gene is first identified as a binding site for retrovirus in murine myeloid leukemia.The human homologue with the murine in leukemia oncogene Evi-1 is about 91％ of nuclear sequence and 94％ of amino acid.Evi-1 gene is high expressed in myeloid malignancies and other tumors.The main tumorigenic mechanisms of Evi-1 gene include inhibiting TGF-β signal path by depressing Smad3,adjusting cell cycle,promoting angiogenesis,inhibiting JNK.activating AP-1,and so on.

Aim: To study the hydrofluoroalkane-driven(HFA-134a) salbutamol sulfate (SS) MDI formulation compositions and the respiratory tract absorption in rats. Methods: Solubility determination and orthogonal design were used to aid the screening of non-CFC SS MDI formulations. Rats were exposed to SS MDI via intubation inhalation. Fluorescent HPLC detection was developed to determine plasma SS concentration in rats given the selected SS MDI formulations. Analysis of the plasma drug level-time profiles was performed by the statistical moment approach. Results: Stable and homogeneous non-CFC SS MDI formulations were obtained. The developed HPLC method was validated and used to assay SS levels in the rats after inhalation of the MDI. AUC ratios of the SS/HFA-134a-driven MDI formulation to the reference SS MDI and CFC SS MDI formulation were 109. 86% and 135. 54%, respectively. Conclusion: It is proven that the absorption of the HFA-134a SS MDI formulation in rats is equivalent to the simulant formulation commercially available oversea and better than CFC formulation.

Pediatric head injury is the leading cause of death and disability,about 40% to 60% of kids of head injury get a CT,and the majority are those with minor head injury,about 10% of these CT scans are positive.Clinical decision rules for pediatric head injury exist to identify children at risk of traumatic brain injury.Those of the highest quality are children's head injury algorithm for the prediction of important clinical events(CHALICE),Pediatric Emergency Care Applied Research Network(PECARN)and the Canadian as-sessment of tomography for childhood head injury(CATCH)clinical decision rules.This review aimed to systematically introduce primary clinical decision rules for children with minor head injury and compare them for diagnostic accuracy in detecting intracranial injury and injury requiring neurosurgery.

Objective To optimize ethanol extraction technologyDingtongning Granule.Methods With yield of extract and content of tanshinoneⅡA as comprehensive evaluation indexes, the concentration of ethanol, the amount of ethanol, soaking time, extraction time and extraction times were selected as observing factors. Orthogonal design test L18(37) was used to optimize ethanol extraction technology ofDingtongning Granule.Results Optimum extraction conditions were as follows: soaking medicine for 0 hour; 2 times with 6 times of the amount of 80% ethanolre; flux extracted 3 hours each time.Conclusion This optimal extraction technology is stable and reliable, which can provide basis for industrial production ofDingtongning Granule.