Positive ratio of chest X-ray is analysed to explore whether it is reasonable chest X-ray in annual physical examination.

Majority of humans requiring radioprotection in medical center are healthy. The most important thing is consummate radioprotection and facilities for environmental protection, and scientific arrangement of staffs and rooms as well.

One hundred Chinese adult humeral heads (50 of either side) were studied with laser. The radius, height, angle as well as the surface area of each were anteriorly, laterally and obliquely measured in two-dimensions.The main results are as follows:1) The mean angle of the articular area was 144.48?11.9?.2) The average radius and height of the humeral head were 2.15?0.16 cm and 1.56?0.24 cm respectively. The articular surface height occupied 36.05 per cent of the diameter of the approximate spherical body.3) The mean articular surface area of the humeral head was 21.02?4.03cm~2. According to the radius of the humeral head and the articular surface angle, the curvature of the articular surface can be estimated and drawn out. That will offer the theoretical data of Chinese adult humeral head for the design and manufacture of artificial joint and artificial humeral head.

BACKGROUND: Experiments have demonstrated that exogenous basic fibroblast growth factor (bFGF) promotes the growth and survival of skeletal muscle satellite cells, and endogenous bFGF also has obvious effect on muscular repair.But whether bFGF gene transfection has the same effect on extraocular muscle satellite cells is unclear.OBJECTIVE: The goal of this study was to investigate the effect of bFGF gene transfection on the bio-behavior of rat extraocular muscle satellite cells.DESIGN: Single sample observation.SETTING: Department of Ophthalmology, Qingdao University Medical College.MATERIALS: Primary rat extraocular muscle satellite cells were cultured (purity ＞ 90%). Human PEGFP-N3-bFGF eukaryotic expression vector was successfully constructed (reported in other papers).METHODS: This study was carried out in the Central Laboratory of Qingdao University Medical College between September 2005 and December 2006. Experimental intervention and grouping: During the experiment, 3 groups were divided: Experimental group, in which, recombinant PEGFP-N3-bFGF was used for transfection; Control group A, in which, empty-load PEGFP-N3 was used for transfection; Control group B: in which, only F 10 medium (0.1 volume fraction of fetal bovine serum)was used for transfection. Recombinant PEGFP-N3-bFGF plasmid was used to transfect rat extraocular muscle satellite cells cultured in vitro by liposome-mediated transgenic technology. Experimental evaluation: bFGF expression was observed by immubohistochemical method; Transfection efficiency was detected with fluorescence microscope; The protein secretion of bFGF of satellite cells in each group was detected by SBC-ELISA method on the 1st, 3rd, 5th, 9th, 11th, 13th, 15th, 21st and 28th days of culture; The proliferation of rat extraocular muscle satellite cells in each group was detected by methyl thiazolyl tetrazolium(MTT) method; Creatine kinase (CK) activity of cells in each group was determined according to the A value of some standard whose concentration was known.MAIN OUTCOME MEASURES: ① Transfection efficiency of bFGF gene to rat extraocular muscle satellite cells cultured in vitro and bFGF gene expression after transfection; ②bFGF protein secretion after transfection, and effect of bFGF gene transfection on the growth and proliferation of extraocular muscle satellite cells.RESULTS: ① Transfection efficiency of bFGF gene to rat extraocular muscle satellite cells cultured in vitrowas (42.8±1.2)%. ② Immunohistochemical detection showed positive reaction. ②Transfected cells could secrete bFGF protein,which was the highest on the 5th day (662.935 ng/L). ④ The proliferation activity of transfected cells was obviously enhanced, and its A value was significantly higher than that of control group at the same time point (P ＜ 0.05). ⑤CK value was higher than that of the control group from the 5th day after transfection to the end (P ＜ 0.01).CONCLUSION: bFGF gene transfering into rat extraocular muscle satellite cells can make extraocular muscle satellite cells to secrete bFGF, promote cell proliferation, survival and differentiation.

As a new type of environmental pollutants, microplastics are widely distributed in the global ecosystem, and ingestion of microplastics may produce a number of toxic effects. Based on currently available publications, this paper describes the main pathways of exposure to microplastics, and summarizes the toxic mechanisms of microplastics in mammals, including oxidative stress, inflammatory response, immune damage, imbalance of gut microbiota, energy metabolism disorder and DNA damage, so as to provide insights into elucidation of the toxic mechanism mechanisms and health risk assessment of microplastics.

Objective To analyze the polymorphic character of HLA-A,B,DRB1 alleles at highresolution level in Ji-nan Han population.Methods A total of 483 unrelated and healthy Han ethnic individual from Ji-nan region were genotyped by PCR-SBT for HLA-A,B and DRB1 loci.Then allelic and haplotypic frequencies were calculated by maximum likelihood estimation method using Arlequin software.Results There were 27,56 and 41 alleles identified at HLA-A,B and DRB1 loci respectively,and the most common alleles were A * 11∶01(0.1615),B * 13∶02(0.1163) and DRB1 * 07∶01 (0.1763).The three loci haplotype was dominated by A* 30∶01-B * 13∶02-DRB1 * 07∶01 with a frequency of 0.0867.Conclusion The HLA-A,B and DRB1 loci are highly polymorphic in Ji-nan Han population.

BACKGROUND:Transgenosis of basic fibroblast growth factor (bFGF) gene has been successfully performed into the muscle satellite cells of rat extraocular muscles in the previous study of the research group, proving that bFGF could express in the myoblasts of extraocular muscles, also promote cell proliferation and differentiation.OBJECTIVE: To further investigate the methods for regulating the expression of the bFGF in myoblasts following transfection. METHODS: Target gene bFGF was connected with inducing expression vector pcDNA4/T0/myc-His?A, followed by masculine clone sequencing identified by colony PCR and enzyme digestion, EcoR I and Hind III restriction enzyme digestion, as well as Xho I single enzyme verification. C2C12 myoblasts antibiotics sensitivity was screened and finally defined. By use of lipofection transfection technology, cell lines where C2C12 stably expressed pcDNA6/TR were estabolishd and then identified by Western blot. The pcDNA4/TO/myc-His?A-bFGF was transfected into pcDNA6/TR- C2C12 cells. The bFGF expression and secretion in C2C12 cells following tetracycline-induced pcDNA4/TO/myc-His?A-bFGF transfection were determined by immunofluorescence and Western blot, the controls were established.RESULTS AND CONCLUSION: ① The conjunction between the bFGF and inducing expression vector pcDNA4/TO/myc-His?A was proved successfully by sequencing comparison, double digestion and single digestion. ②The minimal lethal concentration of blasticidin to C2C12 cells was 10 mg/L, while that of zeocin was 750 mg/L. ③ The pcDNA6/TR-C2C12 cell lines were established correctly. ④ The myoblasts treated by tetracycline and transfected with pcDNA4/TO/myc-His?A-bFGF were positive for gene expression, those untreated exhibited a negativity; bFGF protein could be produced in myoblasts treated by tetracycline and transfected with pcDNA4/TO/myc-His?A-bFGF, the production reached a peak at 24 hours, while those untreated can not produce bFGF protein. Results suggest that the bFGF expression in the myoblasts can be controlled by tetracycline inhibition and regulatory systems.

with PsA in Chinese Han population from Shandong Province.

Objective To identify a novel HLA-B allele in Chinese Han population.Methods No full matched result was obtained in HLA-B locus in HLA typing for China marrow donor program(CMDP) using bi-allelic sequence-based typing(SBT).A confirmatory test for novel HLA allele was performed with mono-allelic SBT.Results B * 15∶05∶01 was confirmed and another allele should be a B * 54new.The B * 54new has 2 nt changes from the closest matching HLA-B * 54∶01∶01 at nt 559,560 where AC→GA(codon163ACG→GAG),led to a coding change,163T→E.Conclusion A novel HLA-B allele was confirmed and officially named HLA-B * 54∶26 under the Genbank Accession number JN209963 by the WHO Nomenclature Committee for Factors of the HLA System in February 2012.

Objective To evaluate the value of MRI in diagnosis of meniscus tear of knee joint.Methods MRI manifestations of meniscus tear of knee joint were analyzed in 60 cases.Four-grade classification of meniscus injury was suggested according to Stoller's classification.Results Meniscus tear was seen in 65 menisci of 60 patients,including 15 medial tear,40 lateral tear,and 5 cases(n=10) of bilateral tear.Fifteen lateral discoid menisci were found among the 60 cases of meniscus tear.MRI revealed a accuracy of 90.8%.Thirty-nine cases were complicated by ligament injury,bone contusion,effusion of joint,fracture and meniscus cyst.Conclusion MRI can accurately diagnose meniscus tear and its complication.