1.Determination of Calcium in Nutritive Food by Ion Chromatography
Journal of Environment and Health 2007;0(08):-
Objective To establish a method for the determination of Ca2 + cations in the nutritive food by ion chromatography.Methods After being digested by the mixture of nitric acid and perchloric acid,the Ca2+ cations in the nutritive food were determined by ion chromatography(IC).Results The optimum chromatographic conditions were studied thoroughly,the linear range was 0.10-500 mg/L,r=0.9998.The lowest detection limit of Ca2+ cations in nutritive food was 0.02 mg/L,RSDs were 0.50%-4.00%,the rates of recovery tested by the standard addition method were in the range of 95.0%-103.0%.Conclusion This method is simple,fast,sensitive and accurate.
2.The efficacy of multimedia visual training system versus that of traditional treatment for amblyopic chil-dren
The Journal of Practical Medicine 2016;32(16):2652-2655
Objective To assess the efficacy and safety of multimedia visual training system for ambly-opic children through a randomized, controlled clinical trial. Methods A total of 203 amblyopic children (253 eyes) ages 3 to 12 years were randomly divided into a study group (100 patients, 170 eyes) and a control group (103 patients, 183 eyes). The study group received multimedia training system; while the control group received conventional integrated therapies. Best corrected visual acuity was examined 3 and 6 months after treatment. The efficacy and safety of multimedia visual training system for amblyopic children was assessed by comparing the ef-fect of treatment in both groups. Results 6 months after treatment, the total effectiveness rate was 74.1% in the study group and 52.5% in the control group, and there was a statistical difference (P < 0.0001). The effective-ness rate for refractive amblyopia was higher in the study group than in the control group (80.5% vs. 50.8%), and there was a significant difference (P < 0.001). As compared with the effectiveness rate for different ages, the subgroups of 3 to 6 years and 7 to 12 years in the study group was 80.8% and 58.0%, much higher than those in the control group (54.9% and 43.9%), and there were statistical differences(P < 0.001 and P < 0.05). Conclusions The efficacy of multimedia visual training system is superior to that of the traditional treatment , especially for refractive amblyopia. It is effective in all age groups.
3.Determination of Dicofol and Pyrethroid Pesticide Residues in Vegetables and Fruits by Gas Chromatography
Hong GUO ; Chuang LIU ; Yuzhu YANG
Journal of Environment and Health 1989;0(06):-
Objective To establish a method to determine dicofol and eight pyrethroid pesticides residues in vegetables and fruits by gas chromatography.Methods The crushed samples were extracted with organic solvent,then the extract was analyzed by DM-17 capillary column gas chromatography after being purified by PestiCarb-NH2 cartridge and silica cartridge solid phase extraction.Results The detection curve covered the linear range of 0.10-2.0 ?g/ml .The minimum detection limit was 0.011-0.090 ?g/kg.The rates of recovery were 85.4%-105%.The variable coefficient(RSD) was under 7.0%.Conclusion This method has the advantages of less disturb, simple and high accuracy, and it can be applicable to the routine analysis of dicofol and pyrethroid pesticides residues.
4.Formulation Improvement and Stability Study of Kangfu Cream
Difeng LIU ; Yuzhu YANG ; Lin CAI
China Pharmacy 2015;(34):4850-4852
OBJECTIVE:To improve the formulation of Kangfu cream,in order to increase the stability of it. METHODS:Ac-cording to the problem that demulsion of zinc oxide and poor stability of Kangfu cream,orthogonal test was used to optimize the formulation of cream matrix with oil phase ratio (stearic acid-white vaseline-glycerol monostearate),with the amount of polysor-bate 80,triethanolamine and SG-10 as factors,using comprehensive score of glossiness,delicacy,consistence,coating and stratifi-cation(10 points as full mark)as index. The stability of the cream was validated and investigated,such as compatibility,heat-re-sisting,cold-resistant,lighting. RESULTS:The formulation were as follows as 100 g cream containing stearic acid 8 g,white vase-line 8 g,glycerol monostearate 6 g,polysorbate 80 4 g,triethanolamine 3 g,SG-10 2 g and appropriate amount of water. The comprehensive scores of the cream were >9.5 points (n=3). Compared with the cream prepared by previous formulation,the cream prepared by modified formulation had no significant change in appearance and color,and demulsion and layering hadn’t been found. CONCLUSIONS:Kangfu cream with better stability than original formulation is successfully prepared.
6.Validity and reliability of the Chinese version of the Devereux Early Childhood Assessment for Preschoolers Second Edition
Yuzhu JI ; Yubai NIU ; Zhidong TANG ; Huiqin YANG
Chinese Mental Health Journal 2015;(7):551-555
Objective:To revise the Chinese version of Devereux Early Childhood Assessment for Preschool-ers,Second Edition (DECA-P2 ),and assess its validity and reliability.Methods:Totally 608 children aged 3 -5 years were rated by their teachers with the Chinese version of DECA-P2 and two criterion scales,which included the Chinese version of Preschool Behavioral and Emotional Rating Scale,and Social Competence and Behavior Evalua-tion Scale-30.Data of 570 were valid.After identifying valid items using item analysis,exploratory factor analysis was performed for half of all samples.Confirmatory factor analysis was also conducted with another half samples.Moreover,the present study tested criterion-related validity,internal consistency reliability and split-half re-liability of the Chinese version of DECA-P2.Results:Through the exploratory factor analysis,the obtained structure of protective factors was the same as the original scale,accounting for 59.82%of the variance.The results of con-firmatory factor analysis achieved the standard of psychometrics (χ2/df=2.50,RMESA=0.07,CFI=0.91,IFI=0.91).Generally,each subscale had significant correlation with the two criterion scales (r=-0.21 ~0.80). Be-sides,the Cronbach αcoefficients of all subscales were between 0.80 and 0.93,and the split-half reliabilities of them were between 0.79 and 0.89.Conclusion:The Chinese version of DECA-P2 has acceptable psychometric quality and can be applied to evaluate resilience for children aged from 3 to 5 years in China.
7.Influence of medicative diet for food therapy on hepatic function of patients with gastric cancer and adjuvant chemotherapy after operation
Liqun YUE ; Yuzhu WANG ; Dongmei HAN ; Xiaoxu YANG ; Fudong LIU
Chinese Journal of Practical Nursing 2008;24(12):4-5
Objective To approach the alleviating effect of medicative diet for food therapy on hepatic function of patients with gastric cancer and adjuvant chemotherapy after operation and supply references for effective treatment and nursing. Methods We divided 123 patients with gastric cancer after operation into the test group (65 cases) and the control group (58 cases).The control group adopted routine treatment project while the test group received medieative diet for food therapy based on routine treatment project. The changes in hepatic functions were compared between the two groups. Results The changes in hepatic functions of the test group were better than those of the control group (P<0.01 ). Conclusion Medicative diet for food therapy could significantly restore the seriously damaged hepatic function after chemotherapy.
8.The changes of procalcitonin level in children with bacterial pneumonia and its clinical significance
Yuzhu HUANG ; Huiquan YANG ; Lulu GAO ; Kaijun ZHANG ; Changliang YUAN ; Kuiwu CAO
Chinese Journal of Primary Medicine and Pharmacy 2014;21(11):1631-1632
Objective To observe the changes and clinical significance of procalcitonin(PCT) level in children with bacterial pneumonia.Methods The levels of PCT,hs-CRP and WBC were detected in 31 pneumonia children pre and post antibiotic treatment,and the changes were observed.Results The PCT levels of the 31 patients were all increased before treatment.After cured,PCT level of 28 cases returned to normal.But in the 28 normal cases,WBC level increased in 10 cases(35.7%),and hs-CRP level elevated in 4 cases(14.3%).In the other 3 cases,the PCT levels decreased from 5.69ng/mL,1.25ng/mL and 0.83ng/mL at admission to 0.51ng/mL,0.55ng/mL and 0.52ng/mL,respectively.And in these 3 cases,there was one case with the levels of WBC and hs-CRP respectively ascended,in which WBC level descended from 13.1 × 109/L to 12.1 × 109/L and hs-CRP level reduced from 20.92mg/L to 7.38mg/L.Conclusion The sensitivity of the index PCT is the highest at the accurate time phase of bacterial pneumonia,which can guide the reasonable use of antibiotics.
9.Study on military-civilian metering of medical equipment
Yuzhu JIANG ; Sai JING ; Xu SUN ; Yang WU ; Weiqin HAO ; Tuo ZHANG
Chinese Medical Equipment Journal 2017;38(5):123-125
Objective To explore the development trend of military-civilian metering of medical equipment.Methods The foundation and conditions were analyzed for military-civilian metering of medical equipment,and the feasibility and necessity were discussed to execute military-civilian medical equipment metering after military innovation.Results The military-civilian metering of medical equipment was expounded from the aspects of organization,mechanism,personnel and etc.Conclution Military-civilian medical equipment metering contributes to rational allocation of national resources and enhancement of military metering.
10.The experimental study on osteogenic potential of goat adipose derived stromal cells induced by PRP with rhBMP-2
Honggang GUO ; Fanglian YAO ; Tao WANG ; Yuzhu LIANG ; Shaoguang YANG ; Qiqing ZHANG ; Yongfa ZHENG
Chinese Journal of Microsurgery 2016;39(2):138-142
Objective To evaluate the feasibilty of modified osteogenic culturing of goat adipose derived stromal cells(ADSCs).Methods From March,2013 to September,2014,platelet-rich plasma(PRP) was made from goat autogenous vein blood,and abodimoneal fat was aspirated,following aspetic procedure,primary and series passage of ADSCs was established.Osteoinduced ADSCs was carried out according to following group:combinative osteoinduction group(PRP+rhBMP-2 +ADSCs),growth factor group(rhBMP-2+ADSCs),conventional inductive group(dexamathasone+ascorbic acid + ADSCs) and noninductive group(blank group).Converted microscope was used to observe cellular pattern,cell activity,osteocalcin and collagen type Ⅰ level were detected at 1,3,5,7,9,13,17,21 days.Red Alazarin and Von Kossa staining were also assayed at different interval.Results Under observation of converted microscopy,remarkable cell proliferation with abundant ECM was noticed in combinative osteoinduction group,compared with other groups,level of cell activity,osteocalcin,collagen type Ⅰ [(0.82 ± 2.19)AU,(79.82 ± 1.36)U/L at,(78.51 ± 4.32)μ g/ml at]were significantly higher than other groups (P <0.05),and remarkable ALP expression and calcifed nodules were also seen.Conclusion PRP can enhance the inductive effect of rhBMP-2,and combinative osteoinduction procedure acts as a satisfactory culturing method.