Objective To discuss the effect and treatment methods of ureteral obstruction caused by primary or metastatic pelvic malignancy. Methods Clinical data of 51 cases with ureteral obstruction caused By pelvic malignancy were reviewed retrospectively. The treatment included traditional open operation in 17 cases in which 3 cases with uretero-uwtero, 9 cases with uretero-bladder anastomotic stoma and 5 cases with cutaneous ureterostomy, retrograde stenting in 19 cases (11 cases with unilateral ureter, 8 cases with bilateral ureter), pereutaneous nephrostomy in 15 cases. Results The median follow-up time was 21 months (range 6 to 72 months ). Three months after operation, uhrasonograph or intravenous urography(IVU)showed that 39 (76.5%) cases had nomal renal function, 12 (23.5%) cases had hydronephrosis relief and renal function improvement. No stricture in the uretero-uretero or uretero-bladder anastomotic stoma was recorded. Conclusions Appropriate treatment is dependent upon the accurate identification of the underlying pathological process and it can relieve ureteral obstruction and increase the quality of life for ureteral obstruction caused by pelvic malignancy.

Objective To investigate the bacterial spectrum and antimicrobial resistance of pathogens isolated from stool of acute diarrhea outpatients ,and provide scientific evidence for clinic rational use of antibiotics .Methods Bacteria was detected by conven‐tional feces culture method ,including separation and biochemistry appraisal sure strains .The predominant bacteria were conducted antimicrobial resistance testing in acute diarrhea outpatients .Results 544 stool specimens were collected from acute diarrhea outpa‐tients from January 2011 to December 2012 .The total positive rate was 17 .83% .Positive rates of Escherichia coli ,Salmonella , Campylobacter ,Vibrio parahaemolyticus ,Other Aeromonas ,Shiga Plesiomonas ,Shigella and Aeromonas hydrophila were 4 .78% ,3 .68% ,2 .57% and 2 .39% ,1 .84% ,1 .28% ,0 .92% and 0 .37% ,respectively .Salmonella ,Campylobacter and Vibrio parahaemolyticus were susceptible to Ofloxacin ,Amoxicillin ,Ceftazidime .They were different resistance to conventional antibiot‐ics ,which were commonly used by clinic ,and the most serious resistance are ampicillin and nalidixic acid .Conclusion Escherichia coli ,Salmonella ,Campylobacter and Vibrio parahaemolyticus are predominant bacteria pathogens .It is important to better under‐stand pathogens spectrum and antimicrobial resistance of bacteria for controlling infection in acute diarrhea outpatients .

Objective To determine the expression profile of serum microRNAs(miRNAs) in early-onset familial Alzheimer's disease (EO-FAD) patients. methods miRNA microarrays were performed to detect the expression profile of serum miRNAs in 2 cases of EO-FAD patients,2 cases of EO-FAD carriers and 2 cases of normal controls.Preliminary bioinformatic analysis was conducted. Result sIt was found that 21 miRNAs were up-regulated and 22 miRNAs were down-regulated in serum of EO-FAD patients,the differences were statistically significant(P<0.05).miR-5704(P=0.0002),miR-4639-3p(P=0.0195),miR-107(P=0.0204) were markedly up-regulated,miR-5572(P=0.0008),miR-204-3p(P=0.0014),miR-542-5p(P=0.0106) and miR-155-5p(P=0.0240) were markedly down-regulated.Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis suggested that the dysregulated miRNAs may be involved in the mechanism of EO-FAD by affecting neurotrophin signaling pathway.Conclusion miR-5704,miR-4639-3p,miR-107,miR-5572,miR-204-3p,miR-542-5p and miR-155-5p may be used as potential biomarkers of EO-FAD,and involved in the mechanism of EO-FAD by affecting neurotrophin signaling pathway.

Objective:To preliminarily compile the words list of personality in southern China, and structurally analyse the words with natural personality traits.Methods:A semi-structured questionnaire was used to obtain 603 valid questionnaires in six provinces in southern China, and the lexical collation, consolidation, deletion, lexicographic analysis and structural analysis were conducted.Results:(1)After three rounds of collation and merging of 8 022 natural words describing personality traits, a personality trait word list containing 136 words was obtained.(2)Econometric analysis of words according to positivity, neutrality and negativity, showed that all negative words involving moral evaluations (ruthless, vicious, evil, cunning, narrow-minded and so on) were used to describe others, while negative words describing oneself were mainly non-virtuous words (capricious, stubborn, impatient, impetuous, pessimistic and so on). (3) The proportion of desirability and virtue dimensions in the words list was highest(23.20%, 16.80%); and the proportion of desirability and extroversion in total words frequency was highest(24.39%, 19.96%).Conclusion:A personality word list containing 136 personality traits in southern China is compiled from primary sources; and a 7-factor personality structure was latent in the word list.Subjects are richer in describing the content of the vocabulary of agreeable, virtuous, pioneering, and persevering personality traits, while agreeable and extroverted personality traits are most easily perceived in interpersonal interactions.

To study the changes of metabolites in rat urine after treatment of Aristolochia fangchi decoction by metabonomic method.

Objective To isolate bacteriophages against extensively-drug resistant Acinetobacter baumannii from hospital sewage and analyze their biological characteristics.Methods Extensively-drug resistant Acinetobacter baumannii isolated from several hospitals in Shanghai during December, 2013 to July, 2014 were used as host bacteria, adopting double-layer agar method to isolate bacteriophages from raw sewage of these hospitals.The phage with broad host range was selected for further study, including observation of electron microscopic morphology, examination of thermal stability, pH stability and the optimal MOI, drawing of the adsorption, one-step-growth and infection curves, as well as sequencing of the phage genome DNA. Results An extensively-drug resistant Acinetobacter baumannii bacteriophage vB_AbaP_PD-AB9 ( PD-AB9 for short) with broad host range was isolated, and electron microscopy revealed it belonged to Podoviridae family.The optimal MOI of PD-AB9 was 0.001.PD-AB9 remained stable among 4 ℃to 50 ℃and pH 4 to 11.In the adsorption experiment, the adsorption rate of PD-AB9 reached above 95%within 5 min.PD-AB9 had a latent period of 4 min and a burst size of 213.PD-AB9 could obviously restrain the host growth, with faster effect at the higher MOIs (MOI=1, 0.1, 0.01) than at the lower ones (MOI=0.001, 0.000 1).Furthermore, genome of PD-AB9 proved to be a double-stranded linear DNA with size of 40 938 bp and GC content of 39.34%.Conclusions PD-AB9 exhibits good thermal stability, wide pH tolerance range, very fast adsorption, a short latent period, a large burst size and it could quickly cause effective host lysis after infection.Therefore, PD-AB9 is promised to act as a new antimicrobial agent to control drug-resistant Acinetobacter baumannii infections and its bio information remains to be further studied.

OBJECTIVE: To determine the role of miR-155 in the pathogenesis of generalized myasthenia gravis (GMG) and the effect of dexamethasone (DXM) on miR-155. METHODS: The expression of miR-155 in B cells from the GMG patients and healthy controls was analyzed by qPCR. The B cells were cultured with DXM and PBS. The B cell proliferation was examined by MTT; CD80 and CD86 frequencies were detected by flow cytometry; and anti-AChRIgG and isotypes anti-AChR-IgG1, 2, 3 in the supernatant were detected by ELISA. RESULTS: qPCR revealed that the expression of miR-155 in the B cells was much higher than that in the controls, and the miR155 expression decreased after DXM treatment. flow cytometry showed that there was no significant difference in the proliferation and the expressions of CD80 and CD86 in the B cells between the DXM group and the PBS group. The concentration of anti-AChR-IgG1 was obviously lower in the DXM group than in the PBS group, but the concentration of anti-AChRIgG, anti-AChR-IgG2, and anti-AchR-IgG3 was similar. CONCLUSION high expression of miR-155 may be associated with myasthenia gravis progression. DXM may disturb the antibody class switch of B cells by suppressing the expression of miR-155 and improve the symptom of MG patients.
Adult ; B-Lymphocytes ; cytology ; immunology ; metabolism ; B7-1 Antigen ; metabolism ; Cell Proliferation ; Cells, Cultured ; Dexamethasone ; therapeutic use ; Female ; Humans ; Immunoglobulin G ; immunology ; Male ; MicroRNAs ; genetics ; metabolism ; Middle Aged ; Myasthenia Gravis ; drug therapy ; genetics ; immunology ; Receptors, Cholinergic ; immunology ; Tetraspanin 28 ; metabolism ; Young Adult

Background: and Purpose Guillain–Barré syndrome (GBS) is an autoimmune-mediated disorder characterized by demyelinating or axonal injury of the peripheral nerve. Our aim is to determine whether serum amyloid A (SAA) is a biomarker of demyelinating injury and disease severity in patients with GBS. Methods: This study retrospectively enrolled 40 patients with either the demyelinating or axonal GBS and sex- and age-matched controls with other neurological diseases as well as healthy subjects. The demographic and clinical features at entry were collected. The serum levels of the SAA isoforms SAA1, SAA2, and SAA4 were determined in the patients with GBS and the controls using the enzyme-linked immunosorbent assay and analyzed for the associations between levels of different SAA isoforms and the clinical features of the patients. Results: The levels of SAA2 and SAA4 were significantly higher in patients with GBS than in both the other neurological disease controls and the healthy subjects (p<0.05 for all). The level of SAA1 did not differ between patients with GBS and the controls. The level of SAA2 was considerably higher in GBS patients with antecedent infection than in those without infection (p=0.020). The levels of different SAA isoforms were not associated with the disease severity or other clinical features of patients with GBS (p>0.05 for all). Conclusions Increased levels of SAA2 and SAA4 may only represent the acute inflammatory status and so cannot be utilized as biomarkers of the disease severity or demyelinating injury in patients with GBS.

Background: and Purpose Guillain–Barré syndrome (GBS) is an autoimmune-mediated disorder characterized by demyelinating or axonal injury of the peripheral nerve. Our aim is to determine whether serum amyloid A (SAA) is a biomarker of demyelinating injury and disease severity in patients with GBS. Methods: This study retrospectively enrolled 40 patients with either the demyelinating or axonal GBS and sex- and age-matched controls with other neurological diseases as well as healthy subjects. The demographic and clinical features at entry were collected. The serum levels of the SAA isoforms SAA1, SAA2, and SAA4 were determined in the patients with GBS and the controls using the enzyme-linked immunosorbent assay and analyzed for the associations between levels of different SAA isoforms and the clinical features of the patients. Results: The levels of SAA2 and SAA4 were significantly higher in patients with GBS than in both the other neurological disease controls and the healthy subjects (p<0.05 for all). The level of SAA1 did not differ between patients with GBS and the controls. The level of SAA2 was considerably higher in GBS patients with antecedent infection than in those without infection (p=0.020). The levels of different SAA isoforms were not associated with the disease severity or other clinical features of patients with GBS (p>0.05 for all). Conclusions Increased levels of SAA2 and SAA4 may only represent the acute inflammatory status and so cannot be utilized as biomarkers of the disease severity or demyelinating injury in patients with GBS.

Background: and Purpose Guillain–Barré syndrome (GBS) is an autoimmune-mediated disorder characterized by demyelinating or axonal injury of the peripheral nerve. Our aim is to determine whether serum amyloid A (SAA) is a biomarker of demyelinating injury and disease severity in patients with GBS. Methods: This study retrospectively enrolled 40 patients with either the demyelinating or axonal GBS and sex- and age-matched controls with other neurological diseases as well as healthy subjects. The demographic and clinical features at entry were collected. The serum levels of the SAA isoforms SAA1, SAA2, and SAA4 were determined in the patients with GBS and the controls using the enzyme-linked immunosorbent assay and analyzed for the associations between levels of different SAA isoforms and the clinical features of the patients. Results: The levels of SAA2 and SAA4 were significantly higher in patients with GBS than in both the other neurological disease controls and the healthy subjects (p<0.05 for all). The level of SAA1 did not differ between patients with GBS and the controls. The level of SAA2 was considerably higher in GBS patients with antecedent infection than in those without infection (p=0.020). The levels of different SAA isoforms were not associated with the disease severity or other clinical features of patients with GBS (p>0.05 for all). Conclusions Increased levels of SAA2 and SAA4 may only represent the acute inflammatory status and so cannot be utilized as biomarkers of the disease severity or demyelinating injury in patients with GBS.