Objective To study the clinicopathologic features and prognosis of low-grade malignant cystic mesothelioma of the peritoneum (LGMCMP). Methods 25 cases of LGMCMP were analyzed retrospectively on their clinicopathologic features and immunohistochemical and electron microscopic findings, therapeutic approach and prognosis were also studied. Results Of 25 cases, 18 were females and 7 were males with a mean age of 39.6 years (range 16-84 years). According to their imaging characteristics, symptoms and physical signs, three morphological patterns of these tumors were found, i.e. localized cystic mass, multiple cystic nodules and diffuse cystic nodules. Immunohistochemical staining showed that the neoplastic cells were positive for CK, Vimentin, EMA, and anti-mesothelial cell antigen. Histochemically, the tumors were positive for CI but negative for HCI. Electron microscopic study of 11 cases revealed that the neoplastic cells had profuse slender microvilli on cell surface, large amount of microfilaments (tono-filaments) and abundant desmosomes. Conclusion LGMCMP is an uncommon neoplasm of low-grade malignancy, generally occurring in young women with distinct clinicopathologic features.

Objective To analyse the clinical and pathological features of cystadenocarcinoma in the intrahepatic biliary tract. Methods The clinical features of 14 patients with cystadenocarcinoma in intrahepatic biliary tract were analysed in respect to the results of ultrasonic and CT scannings, as well as pathological examination. Results Among the 14 patients, 11 were females and 3 were males. The age of the patients ranged from 32 to 79 years, and mean age was 59.5. The obvious symptoms were right-upper abdominal pain and abdominal distention, and major physical signs were hepatomegaly with cystic feeling. The ultra sonographic and CT scanning revealed cystic space occupying lesions in the liver. According to the invasion or infiltration of carcinoma to the liver tissue, the pathology of cystadenocarcinoma in intrahepatic biliary tract could be categorized into two types: the invading and non-invading. There were 8 patients belonging to the first type and they died 9～37 months after the diagnosis or operation, while 6 patients with no invasion were alive after operation. Conclusion The clinical picture and pathology of cystadenocarcinoma in intrahepatic biliary tract were described. The pathological classification was important in prognosticating the outcome of the patient.

cases of focal nodular heperplasia of the liver were studied with HE staining and immunohistochemistry. The patient population was composed of 12 males and 5 females, aged 13 to 71 years. The main symptom was abdomimal pain or discomfort. 9 patients were misdiagnosed as hepatic cell carcinoma (HCC) before operation. Histologically, 14 cases were of classical form, and 2 cases were of telangiectatic form, and 1 case mixed form. The classical form of FNH was defined by the presence of 4 characteristic histologic features: nodular central stellate scar, abnormal nodular architecture, malformed vessels, and cholangiolar proliferation. The causes of focal nodular hyperplasia of the liver were briefly discussed.

Objective To study histological and cytological characteristics of malignant mesothelioma of the peritoneum by needle biopsy. Methods Needle biopsy specimens of 19 cases of malignant mesothelioma of the peritoneum were studied with pathological, cytological, histochemical and immunohistochemical staining examination. Results Of 19 cases, 12 were males and 7 were females, with a mean age of 58.1 years. Histopathlogical examination of needle biopsy specimens revealed that 10 of them were tubulopapillary. 3 undifferentiated. 1 signet ringoid cell, 1 small cell, 2 of low-grade malignant cystic cell and 2 of myofibroblastic cell types. 16 cases were positive by cytology smear but 3 negative. Immunohistochemical staining showed that the neoplastic cells of all cases were positive for keratin, vimentin, EMA, antimesothelial cell antigen and CI but negative for HCI. Conclusion The diagnosis of malignant mesothelioma of the peritoneum can be established by its clinical manifestation, combined with pathological, cytological, histochemical staining and immunohisitochemical staining of specimens obtained by ultrasound guided needle biopsy.

Objective To study the effects and safety of levocarnitine and shuxuetong in treatment of the patients with chronic renal failure.Methods 94 patients with chronic renal failure were selected as research object,and they were randomly divided into group A(levocarnitine and shuxuetong group) 47 cases and group B(shuxuetong group) 47 cases.Group A were treated with levocarnitine oral solution and shuxuetong,and group B were treated with shuxuetong,then the percentage of effectiveness,incidence of adverse reactions of group A and group B and serum BUN,Scr,Hcy were analyzed.Results The percentage of effectiveness of group A was higher than that of group B,but the incidence of adverse reactions of the two groups had no difference,and the serum BUN,Scr,Hcy of group A and group B before the treatment had no differences(P＞0.05),those of group A and group B after treatment were compared there were significant differences(all P＜0.05).Conclusion The effects of levocarnitine oral solution and shuxuetong in treatment of patients with chronic renal failure were better.

Objective To examine the effect of endothelial colony-forming cells conditioned medium(ECFCs-CM)on biological function of human dermal fibroblasts(HDFs). Methods Human cord blood derived-ECFCs were isolated and identified based on the previous studies. The cytokines in ECFCs-CM were detected using a cytokines antibody array. HDFs were cultured with ECFCs-CM,using serum free EBM-2 as control. The proliferation of HDFs was examined by Cell Counting Kit-8(CCK-8)and the migration was assessed by scratch test assay. The apoptosis of HDFs was detected by flow cytometry. Results The cells isolated from human cord blood demonstrated typical characteristics of ECFCs. The cytokines antibody array indicated that ECFCs-CM contained large amounts of secreted cytokines such as PDGF-BBand EGF. Compared with the control group,the HDFs cultured with ECFCs-CM showed improved proliferation and migration ability. The number of apoptotic cells was smaller than that of the control group under the environment of serum starvation. Conclusion ECFCs-CM can promote the proliferation and migration of HDFs and inhibit the apoptosis of HDFs under the environment of serum starvation.

To evaluating the imaging features and the pathological basis of low grade malignant cystic mesothelioma of the peritoneum (LGMCMP). In 14 cases of LGMCMP, radiographic imaging (X ray, CT, and MRI) was reviewed, and diagnosis was established by pathology. The majority of the patients were middle aged women. The main symptoms were abdominal pain or discomfort. CT and MRI revealed solitary cystic masses in 3 of them, multiple cystic nodules in 5, and diffuse cystic nodules in 6. It is therefore concluded that X ray, CT, and MRI are helpful in revealing the characteristics of LGMCMP and confirm the diagnosis.

Objective To know the influence of out-hospital nursing with no gap on the level of HbAlc for patients with diabetes.Methods Divided 80 patients with diabetes into the intervention group and control group randomly,there were 40 cases in the each group.Out-hospital nursing cares with no gap were used in the intervention group,while the routine nursing cares were used in the control group.Fellowed up 1 year.Compared the FPG,2 hPG and HbAlc between the two groups at the time points of the 3rd,6th,9th and 12th month when out of hospital.Results All the indexes which had monitored were better in the intervention group than those of in control group.Conclusions Out-hospital nursing with no gap can remain the indexes in the normal level,and then promote their quality of life,which should be developed in clinical field.

OBJECTIVE: To optimize the formula and preparation technology of gel-matrix sustained release tablet of nicotinic acid(GSTNA).METHODS: The formula of GSTNA was optimized by orthogonal experiment with the amount of hydrophilic gel-matrix material HPMC(K15M,E15-LV) and that of adjuvant calcium hydrogen phosphate(CHP) as factors and with the in vitro release rates as index.Meanwhile,the verification test on the intra-and inter-batch release rates of the samples was performed.RESULTS: The optimum formula could be seen as follows: the ratios of HPMC(K15M,E15-LV) and CHP were 4%,40% and 25% respectively.The GSTNA prepared in this formula achieved a sustained drug release of up to 12 h,and both the intra-batch homogenicity and the inter-batch reproducibility were satisfactory.CONCLUSION: The GSTNA is reasonable in formula and simple in preparation technology.

Objective To study the influence of different dialysis frequence on infection and prognosis of maintenance hemedialysis patients. Methods Fifty-three maintenance hemodialysis patients with chronic kidney disease were divided into 3 groups. Groups A, B, C dialysed thrice per week (19 cases),twice per week (15 cases) and thrice per two weeks (19 cases), respectively. All the patients were dialysed for 1 year. The rate of infection, death number and death reason were recorded. Results All the cases were infected 62 times (17 times in group A, 18 times in group B and 27 times in group C). Pulmonary infection,blood vessels infection, urinary tract infection and digestive tract infection happened 35 times(56.45% ), 14 times (22.58%), 8 times (12.90%) and 5 times (8.06%) respectively. Eight cases died in three groups,and the total death rate was 15.09%, with 1 case in group A,1 case in group B and 6 cases in group C.There was significant difference in prognosis among three groups (χ2 =6.293,P <0.05). Conclusions Patients who dialysis less than twice per week have higher rate of infection and death. Dialysis program should be adjusted and dialysis frequence should be increased.