Objective To investigate the relationship between plasma fibrinogen levels,aldosterion levels and left ventricular mass in patients with essential hypertension.Methods Two hundred and seventy-three patients with essential hypertension were selected as subjects.Eldow vonous blood sample were taken to measure the rennin,aldosterion,fibrinogen,D-dimer,prothrombin fragment 1 + 2 (F1 + 2),and plasminogen activator inhibitor-1 (PAI-1) concentration by radioimmunoassay.And assessed cardiac structure and function in patients ultrasound heartbeat by application of ultrasound heartbeat.According to fibrinogen levels,all patients were divided to low(A group,n =95),medium (B group,n =87) and highest (C group,n =91) groups,and comparative analyzed the general clinical data,biochemical indexes,ultrasound heartbeat graph test results of the 3 groups.Meanwhile to compare the differences between 3 groups of left ventricular mass index on the two different concentrations of plasma aldosterone average ≥ 125 pmol/L or ＜ 125 pmol/L.Results Left ventricular mass index (LVMI) increased in patients with aldosterone levels ≥ 125 pmol/L as the plasma aldosterone increased significantly.The left ventricular mass index in A,B,C group were (35.0 ± 3.1),(45.0 ± 3.6),(51.0 ± 2.9) g/m2 respectively,the difference between the 3 groups have statistical significance (F =6.65,P ＜ 0.01).Within C group,LVMI in patients with aldosterone concentration(≥ 125 pmol/L) was greater than ＜ 125 pmol/L(t =11.65,P ＜ 0.01).Meanwhile the fibrinogen concentration gradually increased in A,B and C group (2630 (2420-2790),3290 (3170-3430),4210 (3791-4580),respectively).Fibrinogen concentration in C group was higher than that in the other two groups (Z =-2.46,P ＜ 0.01).Ultrasonic heartbeat graph examination Results showed that left ventricular diameter in C group was the biggest than that in A and B groups (A,B,C group were (20.0 ± 2.3),(22.0 ± 3.9),(23.0 ± 3.1) mm/m2 respectively,F =8.16,P ＜ 0.05);The same trends were also seen regarding of interventricular septal thickness (A,B,C group were(9.2 ± 1.9),(10.1 ± 2.0),(10.5 ± 2.4) mm respectively,F =10.52,P ＜ 0.01),left ventricular posterior wall thickness (A,B,C group were (8.8 ± 1.5),(9.2 ± 1.6),(9.7 ± 1.7) mm respectively,F =10.6,P＜0.01) and left ventricular mass(A,B,C group were(37 ±9),(43 ± 11),(45 ± 13) g/m2 respectively,F =11.6,P ＜ 0.01).Conclusion Plasma fibrinogen and aldosterone concentrations are associated with left ventricular mass in patients with essential hypertension.Both them of interaction and participate to left ventricular hypertrophy commonly.

Cre/LoxP site specific recombinase system is an effective tool for gene recombination in the body to produce specific gene deletion or inversion.The study of gene function is the experimental basis for the development of experimental animal models.Study the Cre/LoxP system,compare the different function characteristics of mouse models for Cre recombinase system in the research of ophthalmology,epecially in the tissue of retina,such as retinal pigment epithelium (RPE) cells,Müller cells and photoreceptor cells,get the Cre transgenic mouse model to establish proper experimental animal models of ophthalmology will lay a solid foundation for the research of the lack of single gene for a particular organization study.Here,the research progress of Cre/LoxP system,the Cre transgenic mouse models and eye specific Cre recombinant enzyme model mice will be summarized.

Objective To explore the related risk factors between adults with diabetes and cardiovascular disease risk.Methods Five hundred and fifty-seven patients whose age were over 20 years with diabetes mellitus were hospitalized in the First People's Hospital of Shangqiu from Jan.2008 to Dec.2013.According to the major risk factors of cardiovascular risk assessment,Framingham cardiac risk score was use to assess and stratify patients;and then graded as low risk group(＜ 10％),intermediate risk group(10％ to 20％) and the high risk group(＞ 20％).Predicted the next 10 years the probability of the risk of cardiovascular disease.Results Among 557 patients,275 cases were male and 282 cases were female,79 cases (14.18％) were type Ⅰ diabetes mellitus and 478 cases (85.82％) were type Ⅱ diabetes mellitus.Meanwhile,10 years the probability of the risk of cardiovascular disease showed that there were 103 cases of low risk group(18.5％),91 cases of intermediate risk group (16.3％),195 cases of high risk group (30.2％) and 168 cases of cardiovascular disease group who had cardiovascular disease (30.2％).(1) Characteristics of risk factors for cardiovascular disease of risk stratification:①There was high incidence of metabolic syndrome in patients with diabetes mellitus,which was up to 37.9％ even in low risk group and 60.5％ in high risk group.②The average age of high risk group was (63.5 ± 11.7) years old which was higher than the low risk group ((50.3 ± 15.4) years old).③Systolic pressure of high risk group was (143.4 ± 18.1) mmHg,higher than that of the low risk group ((125.7 ± 13.2) mmHg).④It was hard to control the blood pressure in high risk group,so as a few cases(32.3％) were up to the standard.⑤The incidence of chronic kidney disease in high risk group was 21.5％,which was significant higher than that in intermediate risk group(7.7％) and low risk group(7.8％).(2) In high risk groupn,type Ⅱ diabetes mellitus's incidence was 31.6％ which was higher than type Ⅰ diabetes mellitus's(16.4％).(3)In high risk group,the proportion of age range from 70 to 79 years old was up to 39.2％.Conclusion The overall risk of cardiovascular disease increases in patients with type Ⅱ diabetes.Metabolic factors may be the main risk.The risk assessment of diabetic patients will help to increase the intensity of treatment.

Objective To analyze the multi-slice spiral CT (MSCT) combined negative helical CT cholangiography (N-CTC) technical inspection applications in diagnosis of the biliary duct obstruction diseases.Methods Retrospective analysis of clinical data of 72 patients admitted to hospital by MSCT combined with N-CTC technical inspection and confirmed by pathology or surgery in patients with biliary duct obstructive diseases,and compared with pathological findings.Results Seventy-two cases had 81 paragraph obstruction lesions,with the biliary obstruction of pancreatic head to the level of duodenal ampullary were more common,a total of 44 (54.3％,44/81);among the 216 biliary paragraph,the missed diagnosis rate of MSCT combined with N-CTC was 9.9％ (8/81),and misdiagnosis rate of obstruction was 4.4％ (6/135),MSCT combined with N-CTC technology in diagnosis of biliary duct obstruction in comparison with surgical or pathological had no significant difference (P ＞ 0.05).MSCT combined with N-CTC easily missed small low-density stones.Conclusions MSCT combined with N-CTC can clearly positioning biliary duct obstruction and qualitatively,consistency with surgery or pathological diagnosis is good,it is practical,non-invasive,can be used as the first choice for suspected biliary duct obstruction means of inspection,has a wide range of applications.

The phenomenon of insufficient learning motivation is universal among college students,yet especially significant among those scientific students in medical colleges.Based on the investigation of learning motivation among students in science and engineering in medical colleges,this paper analyzes the causes of their insufficient learning motivation and comes up with certain countermeasures.

The diagnostic value of Doppler colour flow image (DCFI) was assessed in a. group of 35 patients with rheumatic matral valvular disease. The conformable rate to the results of operation was 100%, while it is 96% for 50 Patients with congenital heart disease.The study was shown that there was a high degree of diagnostic accuracy of DCFI (r=0.9401) in the quantitative value of 20 patients with VSD by correlation analysis

Objective: To investigate the effect of Xingtong Oral Solution on cardiac hemodynamics and myocardial oxygen consumption in dogs.Methods: The coronary blood flow and cardiac outpout were measured by electromagnetic flowmeter. The internal pressure of left ventricle was determined by carrier amplifier. And the blood oxygen content was also determined by oximeter. Results: Xintong Oral Solution can raise the internal pressure of left ventricle, enhance the work of left ventricle and reduce the peripheral resistance. Conclusion: Xintong Oral Solution can obviously reduce myocardial oxygen consumption and coronary resistance. It is also to give protection to myocardial ischemia.

Objective:To study the best extraction technology of Qishan sugar-free granule. Methods:The effects of boiling dura-tion, boiling times and water addition ratio on the extraction process were investigated by using L9 (34 ) orthogonal design, and the con-tents of total flavonoids, baicalin and berberine were taken as the indices. Results:The optimum conditions were as follows:10-fold a-mount of water for 2 cycles, and every extracting time was 1 hour. Conclusion: The best extraction technology of Qishan sugar-free granule is obtained, and the process is stable and feasible, and the contents of active components are high.

Platelets is the important hemostatic component in the blood and the critical participants in inflammation. It is an important promoting factor during inflammation and can recruit leukocytes and aggregate in sepsis. Decreasing plate?let count was correlated with reverse clinical outcome. Therefore, the value of the platelets examination in clinical monitor was studied by many researchers. Under circumstance of fungal infections, platelets mediate antimicrobial activity and assist dissemination of the fungi synchronously. Regulating interaction between platelets and fungi is difficult. In allergic inflamma?tion patients, the excessive activating of platelets aggravates airway obstruction and worsen pulmonary function. We reviewed current research in activating platelets during inflammation.

This paper analyzes the routine failures and operating flow of full-functional DR-EIDOS3000 and points out the design deficiencies of the detector stand,the elevating table and the console.At last,some countermeasures are suggested for the hidden trouble.