BACKGROUND: Twenty-four hours dynamic electroencephalogram (EEG)is to prolong the time of scanning on the basis of routine EEG to monitor electrical activity of brain in various states, by which, the detectable rate of epilepsy is much improved in children.OBJECTIVE: To probe into the evaluation of 24 hours dynamic EEG monitoring on paroxysmal cerebral disorder in children in epileptic and suspected epileptic patients.DESIGN: Case analysis.SETTING: Department of Neurological Internal Medicine of Second Affiliated Hospital of Jiangxi Medical College.PARTICIPANTS: Totally 151 cases of paroxysmal cerebral disorder were employed, diagnosed in clinic and from the inpatients in Department of Neurological Internal Medicine of Second Affiliated Hospital of Jiangxi Medical College from July 2001 to October 2004, of which, 99 cases were male, 52 cases female, aged varied from 3 month to 14 years. According to clinical diagnosis, two groups were divided, named epileptic group (85 cases) and suspeeted epileptic group (66 cases). Of 151 eases, 39 cases received EEG examination, 21 cases CT scanning examination and 3 cases MRI examination.METHODS: All of participants were inquired medical history in detail,received physical examination in Neurological Department and 24 hours dynamic EEG monitoring [those were determined as epileptic discharge if during the monitoring, it was present spike wave, sharp wave, spike and ware complex, burst high-amplitude slow wave, high-arrhythmic wave,neonatal single rhythmic burst wave and hyperventilation early breakthrough or limitative burst slow wave. Those were determined as non-specific abnormality if it was present abnormal background wave, occipital paroxysmal slow wave of childhood, extreme spindle wave of sleep stage,neonatal diffusive transient sharp wave and occasional non-classic sharpslow complex.]. In addition, the results of routine EEG, CT or MRI examinations were collected.epileptiform discharge of paroxysmal disorder in children during 24 hours of abnormal rate and epileptiform discharge of paroxysmal disorder in children during 24 hours dynamic EEG monitoring with the results of routine EEG, CT or MRI examinations.dynamic EEG monitoring, of 151 cases, abnormality was present in 122cases, of which, 97 cases had epileptiform discharge. The epileptiform discharge rate in epileptic group was higher remarkably in suspected epileptic tic group, epileptiform discharge in dynamic EEG was mainly in unilateral hemisphere or dominant in limitative unilateral side and bilateral cerebral hemisphere. In suspected epileptic group, the detectable rate of epileptiEpileptiform discharge was predominated in sleep period (74%, 72/92), of peared clinic seizure in epileptic group, of which, the detectable rate epileptiform discharge was 93% (13/14), higher remarkably than suspected epileptic group [25 cases of clinic seizure, epileptiform discharge decases received routine EEG examination, of which, 19 cases were present non-specific abnormality and 7 cases had epileptiform discharge; in dynamic EEG examination, 5 cases were present non-specific abnormality and 29 cases had epileptiform discharge. In comparison, non-specific abnormality was dominant in routine EEG and epileptiform discharge was predominated in dynamic EEG.CONCLUSION: Twenty-four hours dynamic EEG examination results in remarkable high detectable rate of epileptiform discharge in diagnosis of paroxysmal disorder in children and determines discharge location and time, indicating fully the advantage of dynamic EEG in evaluation of paroxysmal cerebral disorder in children.

There were three kinds of styles of human sexual ethics in human history, which were styles of deified sexual ethics,of evil and instrumental sexual ethics,and of hedonic sexual ethics. These ethical thought has brought benefit to mankind , but caused unhealthy consequence in theory and practice. We must confirm and establish principles on human sexual ethics by human essences . Only thus can individual and nature, individual and others, individual and society, individual and itself nature (the human body and soul) be developed harmoniously.

Objective To investigate the effects of solution focused approach on the hope level of patients with epilepsy. Methods A total of 83 patients with epilepsy were randomly divided into the observation group and the control group, 42 cases in the observation group, 41 cases in the control group. The control group was given traditional nursing care, the observation group was given nursing intervention based on solution focused approach. Two groups were surveyed with Herth Hope Index (HHI), Self-rating Anxiety Scale (SAS) and Self-rating Depression Scale (SDS) before and after the intervention. Results Before the intervention, HHI of the observation group and the control group scored 24.95 ± 1.92 and 24.56 ± 3.23 respectively. After intervention the scores increased to 28.85 ± 1.81 and 25.31 ± 3.35. After intervention, HHI and each dimension of the observation group scored higher than those before intervention and the control group, the difference was statistically significant (t=-4.75, 5.84, P < 0.01). Before the intervention, SAS scores in the observation group and the control group were 56.41 ± 5.97 and 56.28 ± 6.14, SDS scores were 53.72 ± 8.41 and 53.47 ± 8.26, there was no statistically significant difference between the 2 groups (t=0.09, 0.13, P > 0.05). After intervention SAS scores the observation group and the control group were 41.65 ± 6.22 and 49.72 ± 5.86, SDS scores were 44.57 ± 5.83 and 49.73 ± 6.24, there was significant difference between 2 groups (t=5.67, 3.20, P < 0.01). Intra group comparison of the observation showed that there was significant difference in SAS and SDS scores before and after the intervention (t=10.27, 5.36, P < 0.01). Intra group comparison of the control group also showed statistical difference in SAS and SDS scores (t=4.65, 2.17, P<0.05 or 0.01). Conclusions The solution focused approach can improve the psychological status of patients with epilepsy, improve the level of hope, promote the rehabilitation of patients.

Objective To investigate the expression of activator protein-1 (AP-1) in PBMCs from patients with dermatomyositis (DM) and glucocorticoid effect on the expression.Methods PBMCs were isolated from 15 normal human controls and 20 patients (including 12 patients with primary DM who had never received glucocorticoid treatment and 8 patients with recurrent DM who had stopped glucocorticoid treatment for more than 1 month),and classified into two parts:one was cultured in RPMI 1640 medium with dexamethasone of 80 μmol/L and 10％ calf serum for 48 hours,and the other was frozen at -80 ℃ and reserved.Electrophoretic mobility shift assay (EMSA) was carried out to detect the activity of AP-1 in these cells.Results The expression (grayscale area) of AP-1 in the PBMCs from the normal controls was 4.93 ± 0.15 mm2.A significant decrease was induced by the treatment with dexamethasone in the expression (grayscale area) of AP-1 from PBMCs of patients with primary DM and those with recurrent DM (5.59 ± 0.39 vs.30.23 ± 0.49 mm2,5.69 ± 0.39 vs.34.79 ± 0.61 mm2,F =13812.64,P ＜ 0.01 ).The activity of AP-1 was statistically higher in the PBMCs from patients with recurrent DM than in those from patients with primary DM before the treatment with dexamethasone (P ＜ 0.01 ).Conclusions The enhanced activity of AP-1 may be an important factor inducing the inflammatory reaction in and recurrence of DM.Glucocorticoids may suppress the AP-1 activity to a certain degree.

Objective To investigate the effect of (the transtheoretical model of change,TTM) on the level of self-esteem in children with epilepsy (EP). Methods 65 cases of EP were divided into intervention group with 31 cases and control group with 34 cases by random number table. The two groups were treated with anti EP drug treatment and nursing, the use of the change stage and the Self- Esteem Scale (SES) for the baseline assessment, the intervention group according to the assessment results to develop individual TTM intervention measures, the control group received general health education and psychological care, for a period of 6 months. Two groups of patients were followed up for 6 months after hospital discharge, and the distribution of the changes in the changes of the patients and the change of self-esteem scores were analyzed. Results Before the intervention, two groups in the intention stage, preparation stage, operation stage and maintenance stage self-esteem score comparison, the difference was not statistically significant (P>0.05);6 months after the intervention, the change of phase of self-esteem, the intervention group respectively were (22.61+2.88) points, (22.78+1.56) points, (24.03+1.5) points, (24.39+1.60) points, in the control group were (21.15+2.44) points, (21.39+2.21) points, (22.26+2.33) points, (22.01+2.31) points, with significant differences between the two groups (t=1.12-3.38, P<0.01). Two groups from the former intention phase to maintain the stage of change in the number of children were increased, the intervention group was better than the control group, the difference was statistically significant (χ2=15.00, P<0.01). Conclusions Using TTM as the guidance can improve the self-esteem of children with EP.

Objective To observe the effect of early intervention with conductive education (CE) on high risk infants with cerebral injuries.Methods 98 high risk infants within 3～12 months were divided into control group (n=50) and observation group (n=48). Both groupsreceived routine rehabilitation intervention, and the observation group received CE in addition. The children's mental development cooperationdiagnosis scale (0～6 years old) which edited by Capital Institute of Pediatrics and Institute of Psychology, Chinese Academy of Scienceswas used to test the development quotient (DQ) before and 6 months after treatment. Results The DQ improved more in the observationgroup than in the control group (P<0.001) after 6 months of CE intervention. Conclusion CE can promote the development of high risk infantsin sports, cognitive, speech and social adaptation effectively.

Objective To observe the effects of Conductive Education (CE) on the comprehensive ability of children with cerebral palsy. Methods 100 cerebral palsy children within 3～8 years old were divided into two groups: control group with routine rehabilitation therapy, observing group were applied CE in addition. They were assessed with Gross Motor Function Assessment Scale (GMFM), activities of daily living (ADL) and practical skills for children with cerebral palsy assessment record sheet before and after treatment.Results The scores of GMFM, ADL and practical skills improved after treatment in both groups (P<0.01), but those in the observing group improved more than in the control group (P<0.01). Conclusion CE can improve the motor, self care, communication, sociality, cognize and learning ability in children with cerebral palsy.

Objective To study the correlation between the hope levels and medical coping modes of maintenance bemedialysis(MHD) patients.Methods 118 hemodialysis patients were sampled from a hospital in Fuzhou city in October,2007.A survey was conducted among these patients by questionnaires.And the col-lected data were analyzed with SPSSll.5.Results 98.3% of surveyed patients got a mediate or high-level in hope level.Compared to common patients,MHD patients got a higher score both in wriggly and subdued coping modes,and a lower score in facing mode.The choice of coping modes was affected by the hope levels of MHD patients.Conclusions Pafients with higher hope level tend to adopt wriggly and facing coping modes,while those with lower hope level generally take subdued coping mode.The nurses should help MHD patients seek supports from others in order to increase their hope level and promote them to adopt effective coping modes,in doing 80,it Can promote their mental healthy and improve their qu~ty of life.

c-kit+ cells are mainly derived from bone marrow and cardiac tissue.The cells include various subpopulations.Recent studies have shown that c-kit+ cells are a kind of ideal cells of transplantation therapy for myocardial infarction.However,there is a great debate on the differentiation efficiency of c-kit+ cells towards cardiomyocytes and endothelial cells.Therefore,it is necessary to evaluate the potential of c-kit+ cell differentiation and explore the effects of different developmental stages and microenvironments on differentiation of c-kit+ cells again.These studies could be significant for increasing efficiency of c-kit+ cell transplantation in repairing the infarcted myocardium.

Objective To investigate costimulatory molecules CD80 and CD86 expression in acute myelogenous leukemic cells and clinical implication.MethodsThe expression of CD80 and CD86 in the patients with acute myelogenous leukemia and HL-60 cells,U937 cells,NB4 cells,K567 cells was confirmed by Flow Cytometer.ResultsCD80 was very low or no expression in patients with acute myelogenous leukemia.CD86 expressed in acute myelogenous leukemia (27.86±19.65)%,which was much higher than that in control group[(1.21±0.13)%,t＝3.55,P＜0.01].No significant changes were observed in the expression of CD86 in M4 cells(48.65±21.92)%,M5 cells(39.25±18.67)% and control group(50.20±20.31)%(P＞0.05).After the cells were cultured for 24 h and 48 h,the expression of CD86 was (30.62±5.35)% and (29.43±4.67)% in HL-60 cells ,(24.12±5.23)% and (26.56±6.54)% in U937 cells,and,(21.25±3.78)% and (23.21±6.98)% in NB4 cells (all P＞0.05).The expression of CD80 and CD86 was very low in K562 cells.ConclusionsCostimulatory molecules CD86 expressed in acute myelogenous leukemic cells in the patients with acute myelogenous leukemia and HL-60 cells,U937 cells,NB4 cells.