Objective: A method for the determination of gallic acid in the extract of Quercus salece- na Blume (Fagaceae) and its preparation (Urocalum Capsules) by means of RP-HPLC was established. Method: The hydrolysable tannins contained in the sample is hydrolyzed with hy- drochloric acid, the hydrolysis component-gallic acid is determined by HPLC using RP-C18 column, 0.005mol. L-1 of phosphoric acid-methanol (98: 2) as mobile phase , 270 nm as detec- tion wavelength. Result: The linear range of gallic acid is 0. 3～3. 1?g , r=0. 9999, the average recovery is 100. 4%. Conclusion: It is confirmed that the established method for assay of gallic acid meet the criteria of quantitative analysis of herbal medicine by validation of hydrolysis condition, repeatebility, stability and recovery, The method is simple , good reproducible and precise , and it can also be applied to analysis of other herbal medicine containing hydrolysable tannins.

TLC method for the identification of triterpenoids polyphenols in Fructus Chebulae was established .The results showed that this method had a plentiful chromatogram and good specificity,and can distinguish Fructus Chebulae immaturus from Fructus Chebulae.Determination of gallic acid, one of the hydrolysates of Fructus Chebulae, by HPLC was also reported.The linear range of gallic acid is from 0.17?g to 1.36?g(r=0.9998),and the average recovery is 102.9 %. The results showed that the method is simple, accurate and with good reproducibility.It can be used to determine gallic acid in Chinese herbal medicine that contains hydrolysable tannins.

Objective To study the correlation between the hope levels and medical coping modes of maintenance bemedialysis(MHD) patients.Methods 118 hemodialysis patients were sampled from a hospital in Fuzhou city in October,2007.A survey was conducted among these patients by questionnaires.And the col-lected data were analyzed with SPSSll.5.Results 98.3% of surveyed patients got a mediate or high-level in hope level.Compared to common patients,MHD patients got a higher score both in wriggly and subdued coping modes,and a lower score in facing mode.The choice of coping modes was affected by the hope levels of MHD patients.Conclusions Pafients with higher hope level tend to adopt wriggly and facing coping modes,while those with lower hope level generally take subdued coping mode.The nurses should help MHD patients seek supports from others in order to increase their hope level and promote them to adopt effective coping modes,in doing 80,it Can promote their mental healthy and improve their qu~ty of life.

The rat c-erbA? cDNA fragment was used as probe to investigate the expression of T3 receptor gene in brain, heart and kidney in hyperthyroid and hypothyroid rats. There were three types of c-erbA? mRNA-6. 0kb, -5. 0kb and -2. 0kb in all examined tissues. In heart and kidney, the levels of c-erbA? mRNA decreased in hyperthyroid rats and increased in hypothyroid rats. It might be a compensatory mechanism. In brain, however, the level of c-erbA? mRNA was unaffected by thyroid status. The results suggest that the T3 receptor gene may have an auto-regulatory mechanism in rat heart and kidney under different thyroid status.

Objective To reinvestigate the high performance thin-layer chromatography(HPTLC)method for solving the problem of difficulty in separating between ginsenoside-Re and notoginsenoside-R1,and to establish a specific,fast and economic routine identification and quality assessment method for Notoginseng(San Qi).Methods Chromatographic conditions were as follows:stationary phase-precoated HPTLC silica gel 60 plate(20? 10 cm,Merck);developing solvent system:CH2Cl2-absolute ethanol-water(70 ∶ 45 ∶ 6.5);relative humidity:lower than 18 % ;temperature:10～ 25 ℃ ;derivative reagent:10 % H2SO4 ethanolic solution,heating at 105 ℃ for 3 min and observing the fluorescent chromatogram in a UV cabinet at 366 nm.Results The HPTLC fingerprint consisted of 10 fluorescent bands(peaks in the profile)including ginsenoside-Rb1,Rd,Re,Rg1 and notoginsenoside-R1,which presented the relative consistent ratio of the main peaks generated from the image of the chemical components distribution pattern through scanning by TLC scanner or computer-aided similarity evaluation(CASE)software.Conclusion Notoginsenoside-R1,the specific component of San Qi,is spotlighted in the HPTLC image and separated from ginenoside-Re,which indicates HPTLC method being simple,fast,and effective.The HPTLC fingerprints of 24 batches of samples from different locations(Wenshan of Yunnan,Xinyi of Guangdong)and markets,with different grades show the high chemical stabilities of this Dammarane-type saponins distribution.

Due to the scarcity of resources of Ziziphi spinosae semen (ZSS), many inferior goods and even adulterants are generally found in medicine markets. To strengthen the quality control, HPLC fingerprint common pattern established in this paper showed three main bioactive compounds in one chromatogram simultaneously. Principal component analysis based on DAD signals could discriminate adulterants and inferiorities. Principal component analysis indicated that all samples could be mainly regrouped into two main clusters according to the first principal component (PC1, redefined as Vicenin II) and the second principal component (PC2, redefined as zizyphusine). PC1 and PC2 could explain 91.42%of the variance. Content of zizyphusine fluctuated more greatly than that of spinosin, and this result was also confirmed by the HPTLC result. Samples with low content of jujubosides and two common adulterants could not be used equivalently with authenticated ones in clinic, while one reference standard extract could substitute the crude drug in pharmaceutical production. Giving special consideration to the well-known bioactive saponins but with low response by end absorption, a fast and cheap HPTLC method for quality control of ZSS was developed and the result obtained was commensurate well with that of HPLC analysis. Samples having similar fingerprints to HPTLC common pattern targeting at saponins could be regarded as authenticated ones. This work provided a faster and cheaper way for quality control of ZSS and laid foundation for establishing a more effective quality control method for ZSS.

Objective This study aimed to discuss the different assessment of pain among medical and nursing staff and cancer patients and supply reference for proper analgesic precept for clinical application and nursing measures. Methods We collected the assessment of psychological pain and physiological pain by 55 hospitalization cancer patients, 40 physicians in-charge and 55 nurses in-charge in one week by Johnson inventory. The assessment results were compared and at the same time the relevant problems of the attitude to cancer pain by patients was also investigated. Results Improper recognition existed in cancer pain treatment by most cancer patients. The physiological pain was higher than the psychological pain assessed by both patients and nurses (P<0.05). But the pain assessment by patients was higher than that by the nurses (P<0.05). The assessment of psychological pain was higher than the physiological pain by doctors and both aspects were lower than those by patients, but no statistical difference was seen (P>0.05). The assessment by doctors was more accurate than that by nurses. Conclusions Routine establishment of pain assessment inventory for patients could instruct patients how to record the degree of their pain. We should strengthen the standard training about pain management knowledge and give timely communication with patients' cancer pain.

Objective:To investigate whether the habenula takes part in the depressor effect of losartan.Methods:Arterial blood pressure,heart rate and the unit dischargings of habenular neurons in rat were recorded simultaneously.Results:Arterial blood pressure was apparently decreased by 10 mg/kg losartan intraperitoneally(ip),but heart rate did not change;63.64% (21/33) unit dischargings of habenular neurons was increased in the rate of discharges.Conclusion:The depressor effect of losartan (ip) may be involved in the excitation of habenular neurons by lsoartan(ip).

Objective To establish the fingerprints of quaternary ammonium hydrate alkaloids in Radix Zanthoxyli nitidii by means of HPLC and to identify and evaluate the quality of different parts and commercial decoction pieces of Radix Zanthoxyli nitidii.Method The column of Zorbax Eclipse XDB-C_8(4.6?150mm,5?m)was selected.The mobile phase consisted of A:3 % glacial acetic acid-diethylamine(1000:7.8),B:methanol,and C:acetonitrile(non-lin- ear gradient elution).The elution speed was 0.8 mL?min~(-1),the detection wavelength was at 250 nm and 270 nm,and the column temperature was 20℃.Results The HPLC fingerprint of Radix Zanthoxyli nitidii consisted of 21 peaks which were chiefly composed by alkaloids such as Chelerythrine,Nitidine chloride,with a consistent peak-to-peak ratio.The constituents' distribution information provided quality information for assessing medicinal materials.Conclusion It showed that the alkaloids distributed mainly in the cortex of the roots,so the commercial decoction pieces of aged roots shed cortexes are inferior.The stems can not be used equivalently with the roots due to low content distribution of alkaloids.

AIM:To study functions of pro-domain in tumor necrosis factor-? converting enzyme (TACE) maturation and to develop an approach to interfere with inflammation processes. METHODS: The plasmid pIRES2-EGFP was used to generate the expression plasmids constructed with the signal peptide and pro-domain, full length or without the pro-domain(designated as pIRES2-EGFP/T648, pIRES2-EGFP/T2472, pIRES2-EGFP/T57-T1824,respectively). The plasmids were used to transfect the U937 cells.After the transfected cells were stimulated by LPS, the effect of expression plasmids on TNF-? secretion was detected by ELISA and flow cytometry(FCM).RESULTS: The plasmid pIRES2-EGFP/T648 inhibited 61.09% TNF-? secretion and mediated the accumulation of TNF-? on the surface of U937 cells.The sTNF-? secretion and the level of the mTNF-? in the plasmid pIRES2-EGFP/T57-T1824 transfected cells had no significant deviation compared with the pIRES2-EGFP transfected cells. The sTNF-? secretion of the cells transfected by the plasmid pIRES2-EGFP/T2472 increased and the level of the mTNF-? decreased.CONCLUSION: The pro-domain has dual effects in TACE maturation and might be applied to molecular design of anti-inflammation drug.