ObjectiveTo explore the clinical efficacy of different treatment programs for patients with acute organophosphate poisoning (AOPP).MethodsOne hundred and thirty patients with AOPP were divided into treatment group (80 cases) and control group (50 cases) by table of random digit.The treatment group was given atropine intravenous injection combined with pralidoxime chloride ladder intramuscular injection on the basis of the conventional treatment.The control group was given atropine intravenous injection combined with pralidoxime chloride intravenous drip on the basis of conventional treatment.The healing rate,creatine kinase(CK),aspartate aminotransferase(AST),amylase and C-reactive protein (CRP) were compared between two groups.ResultsThe healing rates of patients with mild,moderate and severe poisoning patients in treatment group were respectively significantly higher than those in control group [ 100.00％(19/19) vs.75.00％(9/12),95.35％(41/43) vs.64.29％(18/28) and 88.89％(16/18) vs.60.00％ (6/10),P ＜0.05].There was no significant difference in CK,AST,amylase and CRP before treatment between two groups(P ＞ 0.05).The CK,AST,amylase and CRP after treatment in treatment group were significantly lower than those in control group [ ( 152.3 ± 23.2) U/L vs.(258.5 ± 22.2) U/L,(37.5 ± 11.4) U/L vs.(44.5± 12.4) U/L,(114.2±43.8) U/Lvs.(147.3 ±61.4) U/L,(5.7±4.1)mg/Lvs.(9.8±5.2)mg/L,P ＜0.05].Conclusions The use of atropine intravenous injection combined with pralidoxime chloride ladder intramuscular injection for mild,moderate and severe AOPP patients is excellent in therapeutic effects,and the clinical cure rates and blood biochemical parameters are more desirable.It is worthy of clinical application.