AIM: Coronary artery stent implantation is a common method to treat coronary artery stenosis disease through catheter. However, its application is limited by restenosis induced by vascular endothelial cell hyperplasia after implantation. In this study, we explored the effect and value of intravascular ultrasound in reducing and identifying restenosis following stent implantation. METHODS: A computer-based online search of Pubmed database and Chinese Journal Full-text Database was undertaken to identify related articles. Forty-four articles were collected, and after first trial, only those about ①application of intravascular ultrasound in coronary stent implantation; and ②evaluation of intravascular ultrasound to the effect of coronary stent implantation. Outdated articles and repetitive studies were excluded. Finally, 13 articles were included. RESULTS: ①Intravascular ultrasound can confirm stenosis extent of target vessel, vessel and vessel lumen cavity size and area to assist to select suitable stent, and guide correct stent implantation. Intravascular ultrasound shows that stent internal diameter should be not less than 3 mm to avoid or reduce stent membrane tearing and plaque prolapse in stent. ②Incomplete expansion of stent after implantation and attachment between stent and vessel wall are main causes for restenosis. Intravascular ultrasound can immediately monitor stent expansion condition, measure stent size after expansion, and identify attachment extent of stent with vessel wall. In addition, intravascular ultrasound can help to know characteristics of restenosis, guide treatment of restenosis, reduce incidence of restenosis and improve stent implantation effect. CONCLUSION: Intravascular ultrasound compensates the deficient of arteriography. Under the guidance of intravascular ultrasound, implanted stent leads to large stent area and low incidence of restenosis. After stent implantation, intravascular ultrasound can accurately detect stent expansion and attachment of vessel and stent, which plays an important role in evaluating restenosis after stent implantation.

Objective　To evaluate the safety of biopsy of the prostate guided by transrectal ultrasound.Methods　Three hundred and seventeen patients suspected to have prostate cancer were examined and 208 patients were followed up.Results　One hundred and eighty-one patients followed up did not feel severe pain during the biopsy of the prostate. Clinical manifestations induded 12 cases of hematuria,4 dases of hematospermia,1 case of fever, 2 cases of pollakisuria and urodymia,2 cases of uroschesis,which disappeared after corresponding treatment.Conclusions　Biopsies of the prostate guided by transrectal ultrasound is advantageous in diagnosis of prostatic carcinoma.

Objective To follow up the intelligence and physical development of 156 infants borne from PIH mothers; to study the measures to improve their ntellectual development and provide basis for the management fo the infant from PIH partiuents. Methods By using the physical intelligential development investigation table we followed up the intellectual and physical development of the infants in PIH group and the normal group at the age of one, two and three years old. Results There were significant differences between PIH gr development ( P

Objective To explore the criterion of constitution with Yin-deficiency and provide the evidence for further development of establishing the criterion.Method According to reference documents on TCM,a questionnaire on the criterion of constitution with Yin-deficiency was developed and distributed to 51 experts of TCM in Zhejiang province.Results The mean scores of forty-six items ranged from 2.02 to 2.78 and top sixteen symptoms were dry mouth,dry stools,dry throat,feverish palms and soles,thready pulse,constipation,emaciation,yellowish urine,red tongue,rapid pulse,vertigo,thirst with preference for cold drinks,vexation,dry lips,little coating,thirst with frequent drinking.Modes of 46 items ranged from 1 to 4.Coefficients of weight for diagnosis ranged from 0.018 to 0.024 and variance of coefficients were from 0.540 to 1.572.Conclusion Forty-six items could become the content of the criterion of constitution.

Objective To compare the accuracy of intraocular lens(IOL)power calculations by using 6 formulas(SRK-T,Haigis,Binkhorst-Ⅱ,Hoffer Q,Holladay-1,SRK-Ⅱ)in different axial lengths of the eye,in order to improve the accuracy of predicating IOL powers for patients after cataract surgery.Design Retrospective comparative clinical analysis.Participants 169 patients(169 eyes)with age-related cataract underwent regular phacoemulsification and posterior chamber IOL implantation.Methods 169 eyes with cataract were divided into five groups according to the axial lengths:AXL≤22mm,22mm28.4mm.The axial lengths and keratometry of the 169 eyes were measured with A-mode ultrasound before operation,and the actual postoperative refraction was measured with the methods of computer and retinoscopy optometry when patients could gain best corrected visual acuity one month after surgery or later.Inputting the axial lengths,keratometry and IOL powers into A-mode ultrasound,the predicted postoperative refraction was calculated with SRK-T,Haigis,Binkhorst-Ⅱ,Hoffer Q,Holladay-1,SRK-Ⅱ formulas,and the differences of predicted and actual postoperative refractions in different axial lengths were compared.Main Outcome Measures The absolute value with the differences of the predicted and actual postoperative refractions,i.e.absolute predicted errors.Results(1)Comparing the difference of predicted and actual postoperative refraction,there was no statistical significance between the five formulas in the groups of axial length(AXL)≤22mm,22mm28.4mm,but the average absolute predicted error of Haigis and SRK-T formulas is significantly smaller than SRK-Ⅱand Binkhorst-Ⅱ formulas.Conclusions In existing sample size,there was no obvious influence on postoperative refraction when using other five formulas except Binkhorst-Ⅱ.But in the extremely long AXL group,Haigis and SRK-T formulas have some ascendancy over the other formulas.

Objective RNA interference (RNAi) expression vector was constructed to inhibit the focal adhesion kinase(FAK)expression in colon carcinoma HT29 cells, and then the sensitivity of the cells to 5-fluorouracil (5-FU) was determined. Methods One specific pair of oligonucleotides with short hairpin and its negative control sequence were designed and synthesized based on FAK cDNA sequences, then they were inserted into pGenesil-l vector to generate the recombinant plasmids. After identification by the restriction endonuclease and DNA sequencing, the recombinant plasmids were transfected into HT29 cells by lipofectamine TM 2000. The stable transfected cells were selected in a medium containing geneticin G418. The change in FAK expression in HT29 cells before and after RNA interference was detected by reverse transcription and polymerase chain reaction (RT-PCR) analysis and SP immunocytochemistry technique. Sensitivity of HT29 cells to 5-FU was determined by MTT assay. Results The recombinant plasmids coincided completely with the designs in the restriction map and the sequence analysis. After FAK being targeted by RNA interference, immunocytochemistry showed the protein expression of FAK was reduced dramatically, and RT-PCR revealed FAK mRNA expression was down-regulated by 76.94% compared to that of untransfected cells. MTT assay also showed that the sensitivity of HT29 cells to 5-FU in transfected pGenesil-1-FAK vector cells was increased, while IC50 declined remarkably (P

Objective To investigate the antitumor activity of Gefitinib, a selected epidermal growth factor receptor-tyrosine kinase inhibitor, on human colorectal cancer cell lines in vitro, and to explore the relationship between the inhibitory effect of Gefitinib on cancer cells and the expression of epidermal growth factor receptor (EGFR). Methods The growth inhibitory effects of Gefitinib, which expressed as the half growth inhibition dose IC50, on colorectal cancer cells were assessed by MTT assay. EGFR mRNA expression was detected by reverse transcriptional PCR (RT-PCR). Western blot was used to determine the expression of EGFR protein as well as its phosphorylated forms (p-EGFR). Results Gefitinib inhibited growth of all the six colorectal cancer cell lines in vitro with an IC50 range from 6.5 to 172.7?mol/L. Lovo cell line, with an IC50 value less than 10?mol/L, was the most sensitive one to Gefitinib, HT29 and SW480 were moderate sensitive to 10?mol/L

Objective To investigate the pathogenic features and antibiotic resistance profile of nosocomial and community-acquired spontaneous bacterial peritonitis (SBP) in liver cirrhosis patients.Methods Two hundred and twenty-six cirrhotic patients with SBP who were admitted to Beijin Ditan Hospital from January 2001 to December 2008 were recruited into this study. The bacterial identification and drug susceptibility were performed. The data were analyzed by Chi square test and t test. Results Eighty-six(38.0% ) patients were diagnosed with nosocomial SBP and 140 (62.0%)were diagnosed with community-acquired SBP. The proportion of Child-Pugh Class C cases in patients with nosocomial SBP was higher than patients with community acquired SBP (97.7% vs. 82.8%; x2= 11. 489, P=0.001). Mortality rate in patients with nosocomiat SBP was also higher than patients with community acquired SBP (50. 0% vs. 30. 0%; x2 =9. 081,P=0. 003). Total 28 species (232strains) of bacteria were isolated from these patients. 77.5 % (69/89) of the nosomial SBP cases and 76.9% (110/143) of community-acquired SBP cases were caused by Gram-negative bacteria (mainly were Escherichia coli and Klebsiella pneumoniae). 19.1% nosocomial SBP cases and 21. 8%community-acquired SBP cases were caused by Gram-positive bacteria. Fungus infections accounted for 3.4% and 1.4% of these two population, respectively(P＞0.05). In patients with nosocomial SBP,19 out of 32 Escherichia coli stains and 5 out of 14 Klebsiella pneunmoniae strains were extended spectrum β-lactamase (ESBL) positive, while among 60 Escherichia coli stains and 32 Klebsiella pneunmoniae strains, only 11 Escherichia coli stains were ESBL positive (P＜0.05). The resistance rates of Gram-negative strains to cephalosporin and quinolone in nosocomial SBP patients were both higher than those in community-acquired SBP patients(P＜0. 05), but all Gram-negative isolates were sensitive to imipenem (P＞ 0. 05). No Gram-positive isolates resistant to vancomycin were found.Conclusions The liver cirrhosis patients with Child-Pugh Class C are vulnerable to nosocomial SBP and the prognosis is poor. Although the pathogenic spectrum are similar in cirrhotic patients with nosocomial and community-acquired SBP, which mainly are Escherichia coli and Klebsiella pneumoniae, the percentage of ESBL producing strains is higher in nosocomial SBP patients compared to that in community-acquired SBP patients.

Objective To study the NF-?B activity in colon carcinoma HT-29 and Lovo cells treated with different crude extracts of Abrotani herba obtained by column chromatography with macroporous resin.Methods The decoction of Abrotani herba absorbed by macroporous resin AB-8 was mounted into the column and then eluted with distilled water and 30%,50%,75% and 95% alcohol.To select appropriate concentrations for treatment,HT-29 cells were pretreated for 24 hours with each elution phase of the rude extracts in different concentrations(0,50,100,200,400 and 600?g/ml),and then their activity was detected by MTT.The HT-29 and Lovo cells were thereafter cultured in DMEM complete media respectively containing distilled water extract and 30%,50%,75% and 95% alcohol extracts in the concentrations as obtained by MTT assay,and the cells in control group were cultured in DMEM only.The cells were then harvested and the nucleic proteins were extracted for measurement with electrophoretic mobility shift assay(EMSA).Changes in NF-?B activity in HT-29 and Lovo cells treated with different concentrations of crude extracts were observed by EMSA.Results Viability of HT-29 cells treated with 400 and 600?g/ml crude extracts were significantly lower than those treated with 0-200?g/ml crude extracts(P0.05).Conclusion Crude extracts of Abrotani herba,extracted by column chromatography with macroporous resin and eluted with 30% alcohol,can inhibit NF-?B activity in colon carcinoma HT-29 and Lovo cells.

The trace elements (Mn, Cu, Zn, Fe) andCalcium, Magnesium in aged person's serum oflong-life area and control area were determined. Itwas found that the contents of Manganese, cop-per, and Calcium of aged person's serum in long-life area were significantly higher than those incontrol area (Manganese and Calcium are essentialelements for health and antiaging, Copper is an es-sential trace element to health). In the long-lifearea the contents of six kinds of elements (Mn,Cu, Zn, Fe, Ca, Mg)in the serum have no signifi-cant distinction between the male aged and thefamle aged. In the long-life area the contents of sixkinds of elements (Mn, Cu, Zn, Fe, Ca, Mg )inaged (61～90) person's serum have no significantincrease or decrease with the increase of age.Judged from serum compositions, the agedperson's serum of the long-life area have a betterspectrum of elements. The relationship betweenthe elements in this experiment and antiaging effect was discussed.