Objective To discuss effect of indwelling bubble technique combined with CO2 laser treatment against condyloma acuminatum on reduction of wound hemorrhage. Methods 400 patients with condyloma accuminatum were divided into the control group and the experimental group with 200 patients in each group, traditional oppression for hemostasis and bubble retention hemostasis were used in CO2 laser treatment against condyloma acuminatum respectively.The effect of each group was compared with χ2 and t test. Results The relapse rate and hemorrhage rate in the experimetnal group was lower than those of the control group. Conclusions With indwelling bubble technique after infiltration anesthesia of local skin in the treatment of condyloma accuminatum by CO2 laser, the operator can work under clear vision and the op-eration quality can be increased.

Objective To estabhsh a rat portacaval shunt model of portal vein arterialization (PVA)by using right renal artery,andtoinvestigateitsinfluence on portal velnpressure,liver funciton and hepatic pathology.Method Forty-three Sprague-Dawley rats were assigned to PVA group(33 rats)and control group(10 rats).Portal pressure,liver function and pathological changes were evaluated at day 2,2 weeks and 2 months after surgery,respectively.Results The experimental model was successfully established in 30 out of 33(91%)rats.At day 2,2 weeks and 2 months after the operation,the portal pressure increased significanfly(P<0.05)compared with the control group,but no significant differences were found in the senrum level of alanine amlnotransferase,total bilirubin,total bile acid and creatinine.There were no patholgiccal changes from day 2 to 2 weeks after surgery,only postoperative expanslon of the sinusoidal snace was found on 2 months after surgery.Conclusion The rat model of PVA by renal artery was successfully established with the hand-suture mierosurgical techniques.The portal vein pressure maintained at a high level after PVA,meanwhile,no negative effect was found on the liver function and hepatic histological structure changes.

Objective To investigate the deep mechanism of Jiawei Shaoyao Gancao Tang (Modified Peony and Licorice Root Decoction) and its medicated serum in treatment of adenomyosis.Methods The cells in ovarian focus tissue were given in vitro primary culture in 6 patients with adenomyosis undergone hysterectomy,and the focus cells were identified by using immunoeytochemistic method.There were 6 groups in this study including high-dose Jiawei Shaoyao Gancao Tang group (high-dose JSGT group,20 mg/mL),low-dose Jiawei Shaoyao Gancao Tang group (low-dose JSGT group,10 mg/mL),Jiawei Shaoyao Gancao Tang medicated serum group (medicated serum group),blank serum group,mifepristone group and blank control group.The focus cells were intervened for 24 h and 48 h respectively in all groups,and then the expression of estrogen (E2) was detected by using enzyme-linked immunosorbent assay (ELISA).The expressions of estrogen receptor (ER) and aromatase P450 (P450arom) were detected by using immunocytochemistic method in all groups.Results The identification results of immunocytochemistic method showed that collected from 6 patients and successfully cultured cells were all focus cells of adenomyosis.The level of E2 decreased significantly in medicated serum group compared with blank control group and blank serum group (P ＜ 0.05).The level of ER decreased significantly in high-dose JSGT group,low-dose JSGT group,medicated serum group and mifepristone group after 24 h (P ＜ 0.05).The total efficacy of decreasing ER was inferior in mifepristone group to that in high-dose JSGT group,low-dose JSGT group and medicated serum group (P ＜ 0.05),and efficacy of decreasing ER was superior after 24 h to that after 48 h in high-dose JSGT group (P ＜ 0.05).The level of P450arom decreased significantly in high-dose JSGT group,low-dose JSGT group,medicated serum group and mifepristone group after 24 h compared with blank serum group and blank control group (P ＜ 0.05),and the efficacy decreasing P450arom was better in high-dose JSGT group,low-dose JSGT group and medicated serum group than that in mifepristone group after 24 h (P ＜ 0.05).The efficacy decreasing P450arom was superior after 24 h to that after 48 h in low-dose JSGT group (P ＜ 0.05).Jiawei Shaoyao Gancao Tang's efficacies of decreasing ER and P450arom were better than mifepristone and these efficacies showed timeliness:the efficacies were better after 24 h than that after 48 h.There was no dose dependence.Conclusion Jiawei Shaoyao Gancao Tang can reduce the levels of ER and P450arom,cut off the positive feedback loop of E2 and P450arom and weaken E2 effect,which may be its mechanism in treatment of adenomyosis.