The high morbidity and compliments of patent ductus arteriosus (PDA) in preterm infants are reported,but the diagnostic and prognostic appraisal of PDA is still debatable.The B-type natriuretic peptide (BNP) and N-terminal pro-B-type natriuretic peptide (NT-proBNP) are found efficient in diagnosis of hemodynamically significant patent ductus arteriosus (hsPDA) in preterm infants,and also helpful in therapy decisions and prediction of treatment response.However,the type of assay and cut-off values have not yet reached a consent,so that further researches are needed.

Objective To evaluate the efficacy of blue-green light phototherapy for jaundice in preterm newborns.Methods Total 124 preterm newborns with birth-weight below 2000 g were included in this study.Infants in group A (gestation age ≤32 weeks or/and instability,n =72) were treated with bluegreen light (group A1 ) or with single side blue light (group A2) randomly.Infants with gestation age ＞32weeks or/and stability (n =52) were also treated with blue-green light (group B1 ) or with single side blue light ( group B2) randomly.Peak levels of serum bilirubin ( PSB),the withdrawal time of jaundice and the duration of phototherapy were compared between groups.Results Group A:PSB of groupAl was lower than that of groupA2 [ (219 ± 31 ) μmol/L vs.( 257 ± 39 ) μ mol/L,P ＜ 0.01 ].The withdrawal time of jaundice group Al was earlier than that of group A2 [ ( 10.3 ±3.0) d vs.( 16.2 ±4.2) d,P ＜0.01 ].The duration of phototherapy of groupA1 was shorter than that of group A2 [ (87 ±34) h vs.(23 ±49) h,P ＜0.01 ].Group B:There were no differences in PSB and withdrawal time of jaundice between two subgroups ( P ＞0.05).The duration of phototherapy of groupB1 was shorter than that of group B2 [ (59 ±23) h vs.(73 ±74) h,P ＜ 0.05 ].Conclusion Blue-green light phototherapy is more effective than single side of blue light in treatment of jaundice in preterm newborns.

Objective To discuss the risk factors for cardiac complications in infants of diabetic mothers (IDMs).Methods One hundred and seventy-one out of 228 IDMs hospitalized in Department of Pediatrics,Shenzhen Hospital of Peking University from Jan.2011 to Apr.2014,were involved,among them,there were 171 cases who had the complete mother's data during pregnancy,and they were received B ultrasonography whin 1 week after birth.All the infants were divided into 2 groups according to echocardiography within 1 week after birth:the observation group (the patients had cardiac complications) and the control group (the patients had normal heart).Conditions of IDMs and their mothers between the 2 groups were recorded and compared.Results In the 171 IDMs,there were 69 cases(38male and 31 female) in the observation group and 102 cases(59 males and 43 females) in the control group.There were 24 preterm infants,16 large for gestational age(LGA) infants in the observation group,while 47 preterm infants and 15 LGA in the control group,and which had no statistical difference between the both groups(x2 =0.129,2.163,1.995 ; all P ＞ 0.05).In the observation group,there were 7 (4.1％) newborns with hypoglycemia,24 (14.0％) women with advanced maternal age,24(14.0％) women with other gestational complications,19(11.1％) women having good control of their glucose serum levels,and 9(5.3％) women with impaired glucose tolerance.All the mentioned factors had statistical differences when compared with the control group[1 (0.6％) case,19 (11.1％) cases,15 (8.8％) cases,80(66.8％) cases,26(15.2％) cases] (x2 =7.752,5.707,9.423,43.735,3.917 ;all P ＜ 0.05,0.01).Logistic regression showed that women with other gestational complications as risk factors(OR =3.29,95％ CI 1.248-8.671),while having a good control of the glucose serum levels acted as a protectable factor(OR =0.098,95％ CI 0.045-0.217).Conclusions IDMs are prone to have cardiac complications if their mothers couldn't control the glucose serum levels during pregnancy,with other gestational complication and advanced maternal age and with more serious diabetes.

Objective:To investigate the biological characteristics of cultured salivary gland cells in vitro. Methods: Submandibular gland cells of rat were primarily cultured and subcultured in DMEM supplemented with ?=10% fetal bovine serum. The growth of the cells was studied by cell counting and MTT assay. The morphological characteristic was observed with microscope and transmission electron microscope.The specific protein expression was investigated by immunohistochemical technic. Results:Morpholgy of the cells was epidermoid with well developed organella and zymogen granules.The cells could subultured to 4～5 passages and kept in culture for 30～40 days.The cells of primary culture and passage 2 grew faster than those of passage 4.The cells were CK8.13, keratin and ?-SMA positive. Conclusion:Rat submandibular gland cells can be primarily cultured and subcultured in vitro.The primarily cultured and first subcultured cells may be more potential in growth.

Objective To discuss the clinicd effect of using nebulised budesonide to treat neonatal laryneal edema caused by mechanical ventilation.Methods 43 newborns with mechanical ventilation were included in this study and divided into two groups:The Observational group(23 newborns)were treated with nebulised budesonide right before excubation once and three times after that,and the control group(20 newborns)were treated with dexamethasone once right before excubation and nebulised adrenine after that for three times.Comparisons were made between two groups about dismissing time of wheeze or hoarseness,tachypnea,PaO2 and PaCO2.Results Observational group:dismissing time of wheeze or hoarseness was(10.26±4.01)hours,dismissing time of tachypnea was(1.63±0.55)days,which was shorter than that of control group[(14.20±5.58)hours/(2.05±0.58)days],P＜0.05).PaO2 and PaCO2 of two groups had no statistical difference(P＞0.05).Conclusion Nebulised budesonide was effective in treatment neonatal laryneal edema caused by mechanical ventilation,using.

Background and purpose:Exploration of the effective early diagnostic and prognostic markers of non-small cell lung cancer (NSCLC) has important scientiifc signiifcance and clinical value. Recently, the role of long chain non-coding RNA (lncRNA) in the tumor attracts widespread attention. This study intended to investigate the level of lncRNA HOTTIP in NSCLC, the effect of HOTTIP on cell proliferation and its mechanisms.Methods:Expression of lncRNA HOTTIP in tumor and their matched non-tumor tissues were determined by quantitative real-time PCR (qRT-PCR) in NSCLC patients. Then, we analyzed the potential correlation of lncRNA HOTTIP expression levels in tumor tissues with clinicopathological features of NSCLC and clinical outcome. The effects of HOTTIP on NSCLC cell proliferation and apoptosis were tested usingin vitro MTT and lfow cytometric assays. Western blot method was uesd to detect the expressions of proteins.Results:LncRNA HOTTIP expression level was signiifcantly decreased in NSCLC tissues in comparison to adjacent non-tumor tissues (P<0.05). It was also proved that HOTTIP expression was associat-ed with NSCLC histological grade and lymph node metastasis. Moreover, knockdown of HOTTIP expression in A549 cell line decreased proliferation and enhanced apoptosis compared with transfected negative control. Western blot assay showed that the level of Bax protein was signiifcantly increased, whereas Bcl-2 was signiifcantly decreased in HOT-TIP-silencing A549 cell.Conclusion:HOTTIP is a novel prognostic biomarker and a potential therapeutic candidate forNSCLC.

Objective: To establish the quality standard for Qiankun Liquid.Methods: Fructus Lycii、Fructus Cnidii、Radix Ginseng in Qiankun Liquid were identified by TLC, and the content of icariin was determined by HPLC.Results: Fructus Lycii、Fructus Cnidii、Radix Ginseng could be identified by TLC. Icariin showed a good linear relationship at a range of 0.126～1.008?g, r =0.9999. Conclusion: The method is accurate and can be used for the quality control of Qiankun Liquid.

AIM: To establish HPLC for the determination of icariin and adenosine in Cordyceps Vigorine Tablet. METHODS:MetaChem Polaris ODS C18-A column(4.6 mm × 250 mm, 5 μm) was adopted; acetonitrile-water was the mobile phase; detection wavelength was set at 272 nm for icariin and at 260 nm for adenosine; column linearity within the range of 0. 374-2. 246 μg for icariin and 0. 348-1. 392 μg for adenosine, respectively. The average recovery and RSD were found to be (99.4 ± 1.84) % for icariin ( n = 6), (99.63 ± 1.29 ) % for adenosine,respectively. CONCLUSION: The method is convenient, fast, reliable to operate and suitable for the quality control of Cordyceps Vigorine Tablet.

Objective To study the relationship between breast symptom, characteristic of ultrasound image and sex hormone level in women with different menstruation status.Methods 50 women with normal men-strual cycle, 129 women in menopause transition and 318 menopausal women were recruited.Breast ultrasound were performed.Breast section thickness, ductal width, breast structure and blood flow were measured.Serum estradiol( E2 ) and progesterone( P) level were measured by enzyme immunoassay on the day when ultrasound was performed.Onset and persistent days of breast pain were recorded.Results ①32(60.4%)women with normal menstrual cycle and 74(57.4%)women in menopause transition women experienced cyclic mastalgia.The aver-age serum E2 level and serum progesterone level were higher in women with cyclic breast pain than in women with-out breast pain.12.5%of menopausal women had breast pain.There was no significant difference of average ser-um E2 level between women with cyclic breast pain and women without breast pain②Breast section thickness was (14.4 ±4.3)mm,(13.0 ±3.7)mm and(10.6 ±3.2)mm, and ductal width was(0.62 ±0.18)mm,(0.88 ± 0.39)mm and(0.90 ±0.47)mm in normal menstrual group, menopause transition group and menopausal group, respectively.The difference had statistical significance.③26 women ( 49.1%) in normal menstrual group had breast structure changes, while 42 cases ( 32.6%) and 60 cases ( 18.9%) in menopause transition group and menopausal group had breast structure changes.Conclusions Most women in normal menstruation and meno-pause transition group experience mastalgia.About 1/3 breast structure change in women of normal menstruation are inversible.About 1/2 breast structure change in menopause transition and menopausal group are similar to those with normal menstruation.

Objective To investigate the clinical value of of procalcitonin detection in neonatal necrotizing enterocolitis (NEC).Methods Sixty cases eligible NEC infants were randomly divided into observation group and control group(n=30 each group).All the infants were divided into Ⅰ grade group(19cases),Ⅱ-Ⅲ grade group(41 cases) according to NEC classification.All infants received PCT detection after NEC diagnosis.The observation group received antibiotic treatment base on the serum PCT levels.The control group received antibiotic treatment according to treatment routine and physician's clinical experience.Results PCT increased in 28 (46.7％) of the 60 NEC infants.In the Ⅰ grade group and Ⅱ-m grade group,the proportion of PCT increased cases were 26.3％,61.3％ respectively,and the PCTmax in PCT positive cases were (0.81±0.25) μg/L,(1.76±0.89) μg/L respectively,the differences were signifiacnt(x2 =4.627,t=-2.608,P＜0.05).The time of antibiotic usage in observation group was significantly less than that in control group ((4.9±3.0) d vs.(8.6±2.5) d,t=-3.645,P＜0.01).The fasting time and the cure time of the two groups had no significant difference (P＞ 0.05).Conclusion PCT detection can help to understand the cause and severity of NEC.Using antibiotics according to PCT levels can reduce the time of antibiotic usage without affecting the effect of the NEC treatment.