Objective This experiment aimed at exploring a limb wound guard to avoid the clothing being stained by bleeding or drainage on llmb wounds, keep them dry and clean inside, enable the limbs to move conveniently and protect the wounds untouched so as to increase patients' sense of security. Methods 180 surgical patients with limb wounds were randomly divided into two groups, 94 cases in the observation group who used limb wound guards and 86 patients in the control group who did not. The treatment effect and comfort degree were compared between the two groups by X2 test and Ridit analysis. Results Statistically sig-nificant differences between the two groups could be observed in the per capita frequency of clothing replace-ment, the wound infec- tion rate, the average days of hospital stay and patients" comfort degree. Conclusions The results indicate that using limb wound guards can prevent the clothing from being polluted by wound exu-clarion, keep them dry and clean inside, make the patients feel comfortable and reduce wound infection, facili-tate the activities of the injured limbs, reduce patients" fatigue and discomfort, protect the wound untouched and therefore, increase pa-tients' sense of security.

Objective To design a anti-slip sheets for patients at fowler's position, to prevent postural discomfort caused by decline and offset of patients' bodies and understand its effects in clinical application. Methods The fowler's position anti-slip sheets for patients were produced based on clinical practice. 120 cases of eligible patients were selected and divided into two groups randomly. In the control group,56 cases used three cross-sectional shakers with bed files and traditional clothing. In 64 cases of the observation group, in addition to the beds conditions of the control group,the fowler's position antislip sheets were added. The effects of the two methods were observed and compared. Results Patients in the observation group were significantly better than the control group in the body decline, upper body offset, bed sheets shrink down, bed stalls injury situation and patients comfort. Conclusions By using of the fowler's position anti-slip sheets, postural discomfort caused by decline and offset of patients' bodies can be prevented effectively, shrink down of the sheets can be prevented to keep clean and beauty of bed sheets,and collision caused by traditional bed files and other complications could be avoided. They are made simply, easy to operate,practical and worth to be popularized.

Objective　To investigate the relation between ovarian blood-flow resistance index (RI) measured with color Doppler ultrasound and the dosage of pituitary desensitization medication in controlled ovarian hyperstimulation cycle.Methods　Thirty infertile females were randomized into group at routine dose of gonadotropin releasing hormone agonist (GnRHa) (group A) and group at reduced dose of GnRHa (group B).Ovarian arterial blood-flow RI was checked on cycle day 8 and on the day of human chorionic gonadotropin ( HCG) administration with transvaginal color Doppler ultrasound.Results　The ovarian arterial blood-flow RI on cycle day 8,the total dosage and the duration of human manopausal gonadotropin (HMG) administration were significantly reduced in group B. In both groups,the ovarian arterial blood-flow RI was significantly decreased in the ovary with more dominant follicles (follicle diameter >10 mm) compared with the opposite one.Conclusions　In controlled ovarian hyperstimulation cycle,compared with routine protocol of GnRHa administration,reduced dose of GnRHa could decrease the ovarian arterial blood-flow RI,the total dosage and the duration of HMG administration without reducing pregnancy rate.

Objective To compare the value of ultrasound and magnetic resonance imaging (MRI) in detecting fetal spine and spinal cord dysplasia Methods Thirty women with complicated pregnancies,age from 22 to 41 years,gestation from 23 - 38 weeks,were studied with MRI within 72 hours after ultrasound studies and revealed 19 cases of fetal spine with irregular,7 cases of vertebral canal widened partly and 4 cases of anomalies of spinal curvature.Autopsy or imaging was conducted when pregnancy was terminated.A close follow-up was given for cases continuing pregnancy within 12 months after delivery.Results (1)Nineteen cases of fetal spine with irregular in ultrasound,the coincidence of antenatal sonographic diagnosis was 42% (8/19),the coincidence of antenatal MRI diagnosis was 89% (17/19).Seven cases of vertebral canal widened partly in ultrasound,the coincidence of antenatal sonographic diagnosis was 0,the coincidence of antenatal MRI diagnosis was 7/7.Four cases of anomalies of spinal curvature in ultrasound,the coincidence of antenatal sonographic diagnosis was 2/4,the coincidence of antenatal MRI diagnosis was 3/4.(2)By MRI study,one cases were conformed their ultrasound diagnosis,eleven cases were completed their ultrasound diagnosis,nine cases were made the same diagnosis as ultrasound and six cases were corrected diagnosis.By MRI and ultrasound study one case of hemivertebra,one case of butterfly vertebra and one case of dorsal dermal sinus were missed diagnosis.Ultrasond had limits and low specificity especially in fetal spinal cord.Conclusions MRI can show the spinal cord and its lesion intuitively and has advantages in displaying fetal anomaly of spine and spinal cord.MRI is a valuable complement to sonography in difficult cases and diagnostic accuracy can be significantly improved.

Objective To evaluate the effects of valsartan and angiotensin-converting enzyme inhibitors on the excretion rate of urinary albumm(UAER) and the clearance of endogenous creatinine(Cr-C) in elderly patients.Methods 120 elderly patients with stage of the 2～3 class hypertension were included.The patients were divided into 3 groups,the valsartan group and the benazpril group and captopril group,the anti-hypertensive effects and UAER and Cr-C were determined before and after the treatment.Results No significantly different anti-hypertensive effects were observed in valsartan,benazpril and captopril.But the effects of UAER,Cr-C in valsartan group had significant changes compared with the other groups,and the three groups had significant differentiation(P

Objective To investigate the changes of carotid, lipidemia and fibrinogen in type 2 diabetes mellitus patients complicated with cerebral infarction (DCI). Methods 58 cases of DCI (DCI group) were enrolled in this study, and 36 cases of cerebral infarction without diabetes mellitus served as control group. The location, number and nature of carotid atherosclerotic plaque and inner diameter of carotid artery were assessed by Doppler ultrasonography in all the patients, as well as blood lipid, blood glucose and fibrinogen were evaluated. Results In DCI group, the rate of carotid atherosclerotic plaque was 79.3%, the IMT and inner diameter of common carotid artery in DCI group were significantly different from control group (all (P

Objective To establish a method for the determination of berberine hydrochloride in Siji Sanhuang capsules(SSC).Methods The RP-HPLC method was performed on Agilent Hypersil ODS column(4.6 mm?150 mm,5 ?m).Acetonitrile and 0.05 mol/L NaH2PO4 water(adjust pH to 3 with H3PO4)were used as the mobile phase(30 ∶70),detection wavelength was at 270 nm,the flow rate was 1.0 mL/min and the column temperature was at 25 ℃.Results In the range of 0.094 to 0.470 ?g,berberine hydrochloride had a good linearity with chromatograph peaks area(r=0.998 3),the average recovery ratio was 98.5 %(n=5),and RSD=2.3 %.Conclusion This method is simple,effective and with a higher sensibility and stability.The method can be used to control the quality of SSC effectively.

Purpose:To clone and construct an eukaryotic expressive vector of ribonuclease inhibitor (RI) gene ,as well as to observe the effects of the transfected pLNCX-ri on the growth of C6 glioma cells.Methods:A segment of RI gene of 1.4 kb was obtained by Nde I/Xho digestion and cloned into pLNCX. Transfective agent and selective antibiotic were lipofect AMINE and G418 respectively. The expression of pLNCX-ri in C6 glioma cells was detected by Western blotting. And SD rats were inoculated by the transfected C6 glioma cells.Results:An eukaryotic expressive vector of RI gene was constructed successfully. RI content was remarkably higher in the transfected cells than that of in the untransfected cells. After SD rats were inoculated by the transfected C6 glioma cells,the tumorigenic time was prolonged, the tumor weight was reduced and the density of tumor vessels was notably decreased. Conclusions:These results indicated that RI gene powerfully inhibited the growth of C6 glioma cells via decreasing tumor vessels formation.

Objective To investigate the effect of antisense VEGF on radiation sensitivity of esophageal cancer cells in vivo.Methods Sixteen male BLBA/C/nu nude mice were randomly divided into 4 groups and prepared cells were injected subcutaneously on paw pat of mice(2?106/100 ?L/mice): The size of the subcutaneous tumors was measured before and after irradiation and transfection.The effect of treatment was evaluated by speed of tumor growth. The expression level of VEGF was examined in different groups by RT-PCR,western blotting respectively.Results The absolute growth delay (AGD)for antisene group, irradiation group and antisene plus irradiation group was 3.0?2.6, 10.1?5.4 and 27.4?3.1 days,and nominal growth delay (NGD) was 24.4?3.1 days. The enhancement factor of antisense VEGF for radiotherapy was 2.41. A decreased expression level of endogenous VEGF mRNA and protein were found in antisene group by RT-PCR and western blotting analysis.Conclusion Antisense VEGF and radiotherapy may enhance radiotherapic effect for esophageal cancer xenografts.

Objective To observe the effect of tyrosine kinase Src on activating Notch-1 protein in pancreatic cancer cell line-BxPC3. Methods Notch-1 expression and c-Src expression were inhibited by siRNA interference, and the activation of Src was inhibited by its inhibitor PP2. The growth rate of BxPC3 cells was measured by MTT. The change of Notch-1 NICD level and Src protein level was measured by Western blot. Results The growth of BxPC3 cells decreased after inhibiting Notch-1 expression, as well as the cells were treated with PP2. The level of Notch-1 NICD decreased both after inhibiting c-Src expression and after Src activation was inhibited by PP2. Conclusion In BxPC3 cells, tyrosine kinase Src promotes the level of Notch-1 NICD, which directly influences the cell growth.