AIM: To investigate the protein expression of cyclin D_2 and p16 in proliferation and differentiation of cultured cardiac myocytes.METHODS: One-day-old Sparague-Dawley rats were used. Cardiac myocytes(CM) were collected by a trypsin-dispersal method and cultured. Cell growth line and fluorescence activated cell sorting (FACS) were used to investigate the proliferation of CM. Ultra-thin sections were made to observe the ultrastructure of CM under transmission electron microscope. The expression of cyclin D_2 and p16 in CM were measured using immunocytochemistry and image analysis.RESULTS: ①Results of cell growth line and FACS analysis showed that cultured CM could proliferate in the first 3 cultured days, but the ability decreased quickly, concomitant with differentiation. CM was obseved quiescent in cell cycle three days later. The ultrastructure of CM showed the large amount of myofilaments and mitochondrion. ②The protein expression of cyclin D_2 in 3,4,5 day CM group was 0.89 times( P

It is the essential characteristic of socialistic society to promote the personal all-around development,and is the fundamental goal of building harmonious socialistic society.If we want to accelerate building our harmonious socialistic society,we must fully recognize the important significance of promoting the personal all-around development in the course of building harmonious socialistic society.And when voluntarily abiding by the general demand of building harmonious socialistic society of the authorities of our party,we should start from promoting people's all-around development and roundly carry out all kinds of educational strategic measures of accelerating building our harmonious socialistic society put forward by the authorities of our party.

Objective To observe the clinical efficacy of different acupuncture methods in treating post-herpetic neuralgia (PHN). Method Eighty PHN patients were randomized into group A, B, C and D, 20 in each group. Group A was intervened by fire-needle acupuncture, group B was by surround acupuncture, group C by pricking and cupping, and group D was by oral administration of Pregabalin. The visual analogue scale (VAS) was observed before and after intervention, and the clinical efficacy was compared. Result Compared to the pre-treatment VAS scores, the VAS scores were significantly changed after intervention and at 30 d after intervention in the 4 groups (P<0.05). The recovery rate and markedly-effective rate were respectively 55.0%and 95.0%in group A, 45.0%and 90.0%in group B, 50.0%and 85.0%in group C, and 40.0%and 60.0%in group D. There were no significant inter-group differences in comparing the recovery rate (P>0.05). The markedly-effective rates of group A, B and C were significantly different from that of group D (P<0.05). Conclusion Fire-needle acupuncture, surround acupuncture, and pricking and cupping are all effective methods in treating PHN;they safe and reliable and can obviously reduce pain intensity.

Objective To investigate the breastfeeding knowledge of neonatal unit medical staff. Methods A purposefully designed anonymous survey was piloted and then distributed to nurses and doctors working in the neonatal unit. Respondents were required to answer twelve single choice and eight multiple choice questions to assess their level of knowledge of breastfeeding. A total of 180 questionnaires were distributed and 135 were returned. Results The average total score of the questionnaire was (61.11 ± 10.85) points. Item ofhow to deal with the thawed milk with not finished after heatingwas highest right rate ,97.8%(132/135);item ofdrug used during breastfeedingwas lowest right rate, only 1.5%(2/135). Conclusions This project has demonstrated that the medical staff is lack of knowledge towards the practice of breastfeeding in neonatal unit and the education of breastfeeding is urgently needed to help the medical staff and guarantee the security of breastfeeding.

ObjectivesTo study the current status of child neglect and its inlfuencing factors in children aged 3-6 years in rural Henan.MethodsScales and evaluation methods in the Chinese rural child aged 3-6 years neglected eval-uation model were applied in this study. The neglect rate and the neglect degree were used to describe the neglect status of rural children aged 3-6 years in Henan. The inlfuencing factors for child neglect were also analyzed. ResultsThe total neglect rate in 450 enrolled children was 37.1% (167/450). The total neglect degree was 51.9±6.5. The boys had signiifcant-ly higher neglect rate than girls (P<0.05). There was no significant difference in neglect degree between boys and girls (P>0.05). There were significant differences in neglect rate and neglect degree among the different age groups (P<0.05), the higher neglect rate and higher neglect degree over age. The neglect rate and neglect degree in nuclear families and fam-ilies with three generations were lower than those in single-parent families and remarried families (P<0.05). The neglect rate and neglect degree in non-only children were higher than those in only children (P<0.05). The left-behind children had signiifcantly higher neglect rate and neglect degree than children living with parents (P<0.05). Based on multivariate logis-tic regression analysis, the risk factors of child neglect were single-parent families (OR = 4.78, 95%CI : 2.10-10.87) and remarried families (OR=4.53, 95%CI : 1.99-10.01). The protective factors of child neglect were parents working on sci-ence (OR = 0.20, 95%CI : 0.10-0.67) and living with parents (OR = 0.03, 95%CI : 0.00-0.54).ConclusionsThe neglect status is serious in Henan rural children, particularly in left-behind children.

Cartilage Diseases ; Ear Auricle ; pathology ; Humans ; Inflammation

Mutations of BRCA1 and BRCA2 are closely related to developing of breast cancer andovarian cancer. There are a great diversity of founder mutations of BRCA1 and BRCA2. Their types and fre quency are greatly different in populations from different geographic regions and ethnicities. In particular ethnicgroups, founder mutations show a high frequency due to a founder effect. Compared with the expensive wholegenomic scan, screening of founder mutations which could provide information for the prevention and treatmentof related cancer is more convenient and easy to popularize with low cost.

Objective To study influence of psychological intervention on life quality of patients with non-erosive reflux disease(NERD) complicated with depression. Methods Patients with NERD and depression(95 cases) were divided randomly into the observation group(48 cases) and the control group(47 cases).The control group received oral administration of rabeprazole,10 mg,bid.The observation group was given psychological intervention based upon routine medication.Tbe reflux diagnostic questionnaire (RDQ), self-rating depression scale(SDS) and quality of life-index(QL-INDEX) scale were adopted for evaluation before and 8 weeks after treatment. Results The score of SDS was (41.58±6.85) in the observation group,which was lower than that of the control group,(50.42±9.26), after 8 weeks of treatment (P<0.01). The score of QL-INDEX was (8.56±1.38) in the observation group,which was higher than that of the con-trol group,(7.98±1.42), after 8 weeks of treatment (P<0.05). Conclusions Psychological intervention for patients with non-erosive reflux disease (NERD) complicated with depression can ameliorate their de-pression state and improve their life quality.

Objective To investigate the effect of the psychological behavior intervention on the mental status and clinical symptom of patients with functional constipation. Methods All of 78 patients with functional constipation diagnosed by Rome m diagnostic code were randomly divided into study group (n =40) and control group (n = 38). The psychological behavior intervention was used in the study group, the caccagogue and symptomatic treatment was used in the control group. The self-rating anxiety scale(SAS),self-rating depressive scale (SDS) was analyzed and the improving of clinical symptom, adverse effect was compared before and after 4 weeks of treatment respectively. Results The scores of SAS and SDS in the study group significantly decreased after treatment [ (36.25±8.61 )scores vs (42.97±9.25 )scores, P<0.01 ;(38.81±6.53)scores vs (43.36±7.18 )scores ,P < 0.01].There was significant difference between the two groups after treatment (P <0.05).The effective rate of study group was higher than that of control group .(97.50% vs 84.21%,P <0.05). The rate of celiodynia, diarrhea and the recrudescence after the course of treatment in control group was 23.68%, 15.79% and 89.47% respectively, while this numerus in study group was 5.00%,2.50% and 5.00%. Conclusions Psychological behavior intervention plays an important role in the treatment of functional constipation. It can improve the mental status, clinical symptom, and decrease the adverse effect.

Objective To investigate the high-risk factors of premenstrual acne to provide evidence for disease prevention and treatment.MethodsA total of 660 patients with premenstrual acne during February 2006 and October 2009 were retrospectively reviewed.Pillshbury method was used for diagnose and classification of premenstrual acne.Six hundred and sixty complete questionnaires were received for statistic analysis.Results Age and season showed no effect on moderate to sever premenstrual acne.However,spicy and fried food(x2=5.68),mental stress(x2=14.58),gastrointestinal disorders(x2=8.07),family history(x2=12.79),longer hairs around areolas(X2=11.88),and thyroid gland,adrenal gland or adnexal cysts(x2=9.72)had significantimpact on moderate to severpremenstrual acne(all P＜0.05).In addition.inflammatory papules on frontal partress were found in 67.4% of moderate premenstmal acne.ConclusionFood intake,mental health,gastrointestinal disorders,family history,longer hair around areolas,and thyroid gland,adrenal gland,adnexal cysts may be high-risk factors of premenstrual acne.