Objective:To provide the basis for postoperative nutritional support,exp lore the appropriate support methods and its influnce on rehabilitation in postoperative patients with laryngea l carcinoma.Methods:285 patients with laryngeal carcinoma were randomly divided into therapy group and control group.The therapy group (161 patients)was given homogenate diet and the control group(124 patients) was given mixed mi lk by nasal feeding 5-6 times per day.The nutritional index and occurr ence of pharyrgeal fistula were monitored at preoperation,7th and 14th days postopera tion r espectively.The duration of hospitalization of each patient was recorded at the same time. Results:The decreasing of the nutritional index w as significantly lower in the therapy group than in the control group(P<0.0 5).The duration of hospitalization was shortented to about 12 days, and the incidence of pharyngeal fistul a was reduced to 11%.Conclusion:The appropriate nutrition may decrease the incidence of pharyngeal fistula,shorten the mean duration in hospital and reduce the economic burden of postoperative patients with laryngeal carcinoma.

Rho(Ras homologue) family play an import ant role in intracellular signal-transduction pathways. Not until recently have they been found to overexpress in many kinds of tumors such as testicular germ cell tumor,colorectal tumor,breast cancer,ductal adenocarcinoma of the pancreas and prostate cancer and been linked to the tumorigenesis and metastasis. So the members of the Rho family of small guanosine triphosphatases would be new marker s for tumor diagnosis、new markers for the prediction of metastatic potential an d new important prognostic factors. Moreover,they have the potential as therape utic targets for the prevention of cancer invasion and metastasis. In this artic le, we review the recent progress in this field.

Objective: To develop the native Chinese Affective Picture System (CAPS) for future research on emotion. Methods: 852 pictures were screened out to make up of CAPS. 46 Chinese university students were collected to rate the valence, arousal and dominance by self-report in a 9-point rating scale for CAPS. Results: The standard deviations of scores on valence and dominance were greater than that on arousal. Scatter plot showed that the score distribution on the dimension of valence and arousal was wide in CAPS. Conclusion: Though IAPS (International Affective Picture System) is highly internationally-accessible, there are still significant differences between the two sources. The native Chinese Affective Picture System is necessary.

The advent of human infections with avian influenza A(H7N9)and the emerging communicable disease has brought us profound thoughts on lemology teaching recently. We analyzed the exiting challenges of lemology teaching, including great changes in communicable diseases com-posing but limited class hours, decreasing of communicable diseases cases, medical students' psycho-logical apprehension, lack of teachers, more and more requirements in the prevention and treatment for communicable diseases from the public, etc. Several suggestions on how to further improve the lemology teaching quality were proposed including adjusting the curriculum arrangement, paing atten-tion to accumulate teaching cases, applying multi-media materials, taking full advantage of the teach-ing cases from specialized hospital, teaching students knowledge about themselves protection, improv-ing the quality of young teachers, emphasizing discipline characteristics and the importance of public health, reforming the teaching method and promoting the effect of teaching in communicable diseases.

OBJECTIVE:To systematically review the efficacy and safety of Shenxian shengmai oral liquid in the treatment of slow arrhythmia,and provide evidence-based reference for clinical treatment. METHODS:Cochrane library,EMbase,PubMed, CJFD,CBM and VIP database were retrieved to collect the randomized controlled trails(RCT)of Shenxian shengmai oral liquid(tri-al group)vs.other Chinese medicines(control group)in the treatment of slow arrhythmia. After quality evaluation,the Meta-analysis was performed using Rev Man 5.14 software. RESULTS:A total of 13 RCTs were included,involving 1 023 patients. The results of Meta-analysis showed that the effective rate [RR=1.26,95%CI(1.18,1.35),P<0.001] and average heart rate [MD=5.80,95%CI (4.01,7.58),P<0.001] in test group were significantly higher than control group;there were no significant difference in the inci-dence of adverse reactions between test group and control group[OR=0.46,95%CI(0.17,1.22),P=0.12]. CONCLUSIONS:Shenx-ian shengmai oral liquid is effective with good safety in the treatment of slow arrhythmia. However,due to low quality of included studies,it remains to be further verified by high-quality and large-sample long-term RCT.

To explore the major risk factors for hepatitis B (HBV) in Shanghai Jinshan district.A one-to-two matched case control study was performed among the residents of Shanghai Jinshan district in 2010.The data were analyzed by single factor and multivariate analysis performed with SAS 9.13.Single factor analysis showed that the risk factors for hepatitis B were family member with hepatitis B,no vaccination history of hepatitis B virus vaccine and oral treatment.Muhivariable analysis showed that family member with hepatitis B (OR =1.91),no vaccination history of HBV vaccine (OR =0.49) and oral treatment (OR =2.06) were independent risk factors for HBV infection in Shanghai Jinshan district.Strengthening health education for HBV prevention,modifying unhealthy living habits and standardizing the treatment of oral diseases are important measures for lowering infectious rate of HBV.

AIM: To investigate the effect of glucocorticoid receptor beta(GR?) on the transcriptional activation function of glucocorticoid receptor alpha(GR?) and elucidate the biological significance of glucocorticoid receptor beta. METHODS: GR? (pRSH-GR?) and GR? responsive reporter gene cat (pMMTV-cat) were cotransfected into human osteosarcoma cell line (HOS-8603). Transient transfectants were treated with dexamethasone and the activity of cat was determined by the method of ELISA. The expression p21 messenger RNA was detected by the method of reverse transcription-polymerase chain reaction. RESULTS: GR? repressed the expression of reporter gene-cat in a dose-dependent manner. The expression of messenger RNA of p21, an endogenous target gene of GR?, was also inhibited by GR?. CONCLUSION: GR? can inhibit the transcriptional activation function of GR?. GR? may be an endogenous inhibitor of GR?.

AIM: To explore the possible role of p21 wafl gene in the effect of 1,25-dihydroxyvitamin D 3 on a human osteosarcoma cell line (HOS-8603) proliferation and differentiation. METHODS: The effect of 1,25(OH) 2D 3 on the expression of p21 wafl mRNA was determined by quantitative reverse transcription-polymerase chain reaction. The human osteosarcoma cell line stably expressing the p21 wafl mRNA, which named HOS-p21 wafl , was constructed by lipofectamine transfection, and the expression of p21 wafl protein was detected by Western blot. Then the growth curve of the HOS-p21 wafl cells and the expression of alkaline phosphatase (AKP) in the HOS-p21 wafl cells were investigated. RESULTS: The expression of endogenous p21 wafl mRNA in HOS-8603 cells was induced by 1,25(OH) 2D 3 in a time-dependent manner, reaching the maximum at 4 h, and remaining the inducible action for up to 24 h over basal levels. The HOS-p21 wafl cells grew much slower than the control cells, only reaching 50% of the control ones when cultured for up to 6 days. Histochemical analysis showed that the expression of alkaline phosphatase (AKP) in the HOS-p21 wafl cells increased remarkably.CONCLUSION: These results suggest that the p21 wafl mRNA expression induced by 1,25(OH) 2D 3 is one of important mechanisms responsible for the cellular effects of the hormone on HOS-8603 cells.

Objective To investigate the association of transforming growth factor (TGF) gene rs1800469 (TGF1-509C/T) and rs1982073 (TGF1+869T/C) single nucleotide polymorphisms (SNP) with susceptibility of lung cancer. Methods The case group consisted of 193 histologically diagnosed lung cancer patients, and control group of 211 controls were selected from cancer-free patients at the same time. The single nucleotide polymorphisms of rs1800469 (TGF1-509C/T) and rs1982073(TGF1+869T/C) were analyzed by real-time fluorescent quantitative reverse transcription polymerase chain reaction method. The demographic characteristics, correlated risk factors of exposure and SNP between 2 groups were compared. Results The genotype distribution frequency of TGF1+869T/C and TGF1-509C/T in control group was accorded with Hardy-Weinberg law (P>0.05). The TT, TC and CC genotype distribution frequency in TGF1+869T/C in case group were 16.1%(31/193), 55.4%(107/193) and 28.5%(55/193), while these in control group were 22.3% (47/211), 50.2% (106/211) and 27.5% (58/211), and there were no statistical differences (P>0.05). The TT, TC and CC genotype distribution frequency in TGF1-509C/T in case group were 28.0%(54/193), 56.0%(108/193) and 16.1%(31/193), while these in control group were 27.0% (57/211), 51.2% (108/211) and 21.8% (46/211), and there were no statistical differences (P>0.05). The allele in rs1800469 (TGF1-509C/T) and rs1982073 (TGF1+869T/C) showed no association with lung cancer (P>0.05). Conclusions TGF gene SNP may not be associated with susceptibility to lung cancer in Han Chinese in North China.

BACKGROUND:Previous studies have suggested that the risks for coronary atherosclerotic plaque progression and in-stent restenosis are increased in patients with coronary heart disease combined with type 2 diabetes. OBJECTIVE:To explore the predictive factors for in-stent late loss and non-culprit coronary lesion progression in patients with type 2 diabetes mel itus. METHODS:A total of 399 stenting patients were enrol ed, including 179 diabetic patients and 220 non-diabetic patients. The clinical materials, angiography parameters and biochemical markers were col ected. The difference between the two groups was compared, and also we conducted subgroup analysis in the diabetic patients. Low-density lipoprotein cholesterol, hemoglobin A1c, fibrinogen and high-sensitivity C-reactive protein were detected at days 3, 120, 210 and 360 after stenting. RESULTS AND CONCLUSION:Compared with non-diabetic patients, the stent length (P=0.18) was longer and the stent diameter (P=0.002) was smal er in the diabetic patients. The minimal lumen diameters of post-procedure and fol ow-up angiography in the diabetic group were significantly decreased (P=0.001, P=0), and the diabetic patients also showed severe coronary artery stenosis instantly and within the fol ow-up after stenting (P=0.038, P=0.004). The fol ow-up angiography showed that the diabetic patients had more late loss and restenosis (P=0, P=0.097). Furthermore, in the subgroup analysis of diabetic patients, the levels of hemoglobin A1c, fibrinogen and high-sensitivity C-reactive protein were significantly increased in the patients with restenosis and non-culprit lesion progression. These findings indicate that diabetic patients appear to have the higher incidence of restenosis and non-culprit lesion progression. Moreover, hemoglobin A1c, fibrinogen and high-sensitivity C-reactive protein are effective predictors for in-stent late loss and non-culprit coronary lesion progression.