Objective] Introducing the research on acupoints compatibility in recent ten years in order to discuss the research ideas. [Methods]Through retrieval of all medical journals of CNKI from Jan.2004 to Sep.2014,thirty-four articles were selected and analyzed. Summarizing and commenting the progress on compatibility mode, mechanism and action nature aspects.[Results]The research has summarized the characteristics of acupoints compatibility on ancient acupuncture literature and researched the clinical application of classic compatibility, discussed the mechanism of acupoint compatibility on the metabolism of biological molecules, the spectrum of endogenous metabolites and the central mechanism, confirmed the synergic and antagonistic effects of acupiont compatibility. There are some progresses but also deficiencies. [Conclusion]The acupoints compatibility research should change into clinical application, take multi discipline research methods and use different ideas. The relationship between acupoints quantity and effect of compatibility must be a research direction.

Objective: To investigate the efficacy of the green tortoise exploring point technique for treating piriformis syndrome. Methods: Ninety patients were randomly allocated to a green tortoise exploring point treatment group of 30 cases, an acupuncture control group of 30 cases and a hydro-acupuncture control group of 30 cases for clinical observation. Results: The symptoms improved after treatment in all the three groups. The total efficacy rate was 100.0% in the treatment group, 76.7% in the acupuncture group and 83.3% in the hydro-acupuncture group. There were statistically significant differences between the three groups (P<0.01, P<0.05). Conclusion: The technique of green tortoise exploring point is better than conventional acupuncture and hydro-acupuncture in treating piriformis injury syndrome

The molding of humanistic spirit in medical colleges and universities has a direct bearing on whether or not the cultivated medical students can establish fundamentally the mental idea of humanistic solicitude and orientation of value.This article expounds the importance and urgency of strengthening the molding of humanistic spirit in medical colleges and universities,and analyzes the current situations of construction of humanistic spirit in colleges and universities of our country at present,and investigates the ways and countermeasures of strengthening the molding of humanistic spirit in medical colleges and universities.

Objective:To investigate the expression of estrogen receptor in genioglossus of female rats. Methods:30 female Sprague-Dawley rats were randomly assigned to three groups: normal group(NORMAL), sham operation group (SHAM) and ovariectomized group(OVX). The rats were sacrificed 6 weeks after surgery, and the genioglossus was removed for analysis. The expression of estrogen receptors(ER? and ER?) was examined by immunohistochemical assay. Results:ER? and ER? were found in genioglossus of normal rats. The expression of ER? was significantly higher than ER? (P0.05). Conclusions:Estrogen receptors are expressed in genioglossus of female rats.ER? expression is significantly impacted by the estrogen level.

BACKGROUND:Both in vivo and vitro microenvironment can influence the proliferation and differentiation of neural stem cells (NSCs). In addition, cell culture solution plays variable roles in cell proliferation and differentiation.OBJECTIVE:To develop a convenient and rapid method to promote NSC primary culture by modifying traditional serum-free medium. DESIGN, TIME AND SETTING:Randomized controlled cell trial was performed at Institute of Brain Science, Qingdao University Medical School from November 2005 to September 2006.MATERIALS:Ten Wistar rat of gestation for 12-16 days; cell suspension fron brain tissue of embryonic rat. METHODS:Cell suspension were seeded into four 50-mL culture flasks with cell density of 1×106 mL-1, and divided into 2 groups with 2 flasks in each group. The control cells (flasks A and B) were cultured in serum-free medium containing DMEM/F12, B27 (2%), basic fibroblast growth factor (20 μg/L), and epidermal growth factor (20μg/L), and the experimental cells (flasks C and D) were firstly cultured with DMEM/F12 containing fetal bovine serum (FBS, 5%), following by the same serum-free medium 2 to 3 days later. MAIN OUTCOME MEASURES:The proliferation of NSCs was observed by inverted microscopy and immunocytochemistry.RESULTS:①Most of the cells in two culture conditions expressed nestin, the specific marker of NSCs, and were immunocytochemically positive. ②Neural stem cells cultured with FBS formed neurospheres 3-4 days earlier than those without FBS. ③There were no significant differences in cell number, but the neurospheres under the experimental condition were larger than those in control group. Some of the cells were neurone specific enolase-positive after serum-conditioned induction, and some were glial fibrillary acidic protein-positive, indicating NSCs had differentiated into neuron-like cells and neurogliocytes.CONCLUSION:FBS-conditioned pre-culture can accelerate the proliferation of neural stem cells.

Objective To investigate the effect of standardized nursing intervention on airway inflammatory reaction evaluation by testing fractional concentration of exhaled nitric oxide in asthma patients.Methods 62 asthma patients were randomly divided into the intervention group(32 cases)and the control group (30 cases).Two groups were performed FENO determination before and after the treatment.The control group was given routine method for determination,the intervention group was given nursing intervention on the basis of conventional methods.The comfortable situation,satisfaction degree,treatment efficacy were determined between the two groups.Results The comfortable situation,satisfaction degree,treatment efficacy of the intervention group were better than those of the control group.Conclusions Standardized nursing intervention can eliminate the adverse interference in the FENO determination and improve the accuracy of the determination.It can also improve the clinical symptoms of patients with asthma and relieve airway inflammation.This method deserves to be recommended.

Objective To explore the method of comfort nursing in patients with nasogastric feeding and observe the influence on enhancement of patients satisfaction degree. Methods Case control study Was used in 162 conscious patients with nasogastric feeding.80 patients from January 2005 to January 2006 were selected as control group and given routine nursing.82 patients from February 2006 to May 2007 were named as the test group and received confort nursing.The satisfaction degree of patients, injury of nasal mucosa, shedding rate of the gastric tube and aspiration rate were investigated with self-designed discomfort questionnaires. Results The satisfaction degree in the test group Was 63.41％,which was higher than that of the control(40.00％,P＜0.01).The rate of nasal mucosa injury, shedding of the gastric tube and aspiration rate were lower in the test group(1.22％,2.44％,1.22％)than those in the control group(8.75％,10.00％,7.50％,P＜0.05).Conclusion The application of comfort nursing in patients with nasogastric feeding contributed to the enhancement of satisfaction degree of patients and nursing quality.

Objective To investigate the changes of CT perfusion (CTP) imaging and the risk factors for CTP abnormality in patients with transient ischemic attack (TIA).Methods TIA patients were enrolled; CTP and CT angiography were performed.The parameter values of cerebral blood flow (CBF),cerebral blood volume (CBV),mean transit time (MTT),and time to peak (TTP) were obtained.CTP abnormalities were identified by comparing the contralateral mirror area.Demography and baseline clinical data were compared in a CTP imaging abnormal group and a normal group.Multivariate logistic regression analysis was used to identify the risk factors for CTP abnormality in patients with TIA.Results A total of 69 patients with TIA were enrolled,and 52 of them had perfusion abnormalities corresponding to clinical symptoms.Their TTP and MTT were prolonged,but the decreased CBF and CBV was not obvious.Multivariate logistic regression analysis showed that the higher National Institutes of Health Stroke Scale (NIHSS) score (odds ratio [OR] 1.991,95％ confidence interval [OR] 1.113-3.564; P=0.020),the longer duration of symptoms (OR 1.062,95％ OR 1.013-1.114; P =0.013),and intracranial vascular stenosis (OR 15.410,95％ OR 2.118-112.116; P=0.007) were the independent risk factors for CTP abnormality in patients with TIA.The correlation analysis showed that the prolonged TTP (r =0.389,P =0.001) and MTT (r =0.413,P=0.001) were significantly associated with the NIHSS score.Conclusions TTP and MTT may sensitivity reveal cerebral perfusion abnormalities in patients with TIA.The more severe the disease is,the higher the CTP abnormal rate will be.

Ricin is a plant toxin isolated from the seed of the castor plant(Ricinus communis). As a typical II ribosome inactivating protein,ricin consists of two polypeptide chains named ricin toxin A chain(RTA)and ricin toxin B chain(RTB),linked via a disulfide bridge. RTB binds to both glycopro?tein and glycolipid at the surface of the target cell and mediates ricin to be endocytosed and transported retro?gradely to the endoplasmic reticulum. After being reduced and retrotranslocated to the cytosol,RTA mediates its toxicity due to its activity of a RNA N- glycosidases. Aside from its main toxic effect of protein synthesis inhibition,ricin also displays other properties that contribute to its toxicity such as inducing apoptosis,cytokine secreting and peroxidation. Ricin is stable and can be easily isolated. It has many routes of intoxication with no specific antidotes. Due to its natural abundance,remarkable toxicity,and the potential to be used in biological warfare as well as terrorist attacks,ricin has been classified as a Category B biothreat agent. Here we reviewed its history as a biothreat agent,constitu?tion,intoxication mechanism,detection methods and the development of specific antitodes.

AIM: To explore the possible role of the vitamin D receptor (VDR) in the effect of 1,25-dihydroxyvitamin D 3 and its novel analogues on a human osteosarcoma cell line (HOS-8603) proliferation. METHODS: Detecting the VDR mRNA and protein expression in HOS-8603 cells by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical analysis, respectively. Using the method of transient transfection of a reporter gene (DR3-tk-CAT) for VDR to detect its function. The cell VDRas3 which stably expressed VDR antisense mRNA was used to observe the effect of 1,25(OH) 2 D 3 on the proliferation of HOS-8603 cells and the induction of p21 mRNA, one of the VDR target genes, when the VDR in the cells was blocked. RESULTS: HOS-8603 cells expressed the vitamin D receptor which functioned as a hormone-dependent transcriptional factor. When VDR in the HOS-8603 cells decreased, the cells lost the responsiveness to 1,25(OH) 2 D 3 irrespective of either the induction of p21 gene expression or the cell proliferation. CONCLUSION: The effect of 1,25(OH) 2 D 3 on the proliferation of the human osteosarcoma cell line HOS-8603 was mediated by its nuclear receptor VDR.