Objective Polysaccharide from Sargassum thunbergii Kuntze(PST) was purified and degraded to study its anticoagulation activity.Diatomite was used for decolourization and NaCIO for degration of the polysaccharide to get three degraded products.Methods PST and the degraded products were applied in mice anticoagulation experiment by methods of cutting tail and capillary.Results All samples showed significant activities to extend bleeding time and blood coagulation time in mice,as well as to increase the amount of bleeding in mice.Conclusion Polysaccharide from Sargassum thunbergii Kuntze has significant anticoagulative activity and its anticoagulative activity is closely related with its molecular-weight.

Based on the framework of new institutionalism,the paper defined the concept and history of public hospital′s attributes,dividing such hospitals into those built based on poverty alleviation and those on social identity priority.Considering the attributes of the public goods provided by public hospitals,the diversity of their investment,and attributes of their value production process,this paper probed into their property right arrangement.It is emphasized that such hospitals should enhance their key property right feature of public ownership,and on such basis their relationship with government and corporate governance establishment can be discussed.

The processing methods of plant nematode slides were introduced in this paper,including temporary mounts,permanent mounts and the stain method for nematode.Each method was described step by step in detail.These methods were essential to the study on classification,identification and characteristics of the plant nematode.

Classification of plant nematodes was summarized in this paper.Points of view on taxonomy of plant nematodes were listed.Some serious plant diseases which were infected by plant nematodes were involved.Identification of genus was introduced,including Meloidogyne,Heterodera,Ditylenchus,Aphelenchoides and Bursaphelenchus.

Objective To study the clinical effect of elastic intramedullary nail in the treatment of preschool children limb fractures.Methods Ninety-two 92 cases closed fracture of extremities of preschool children in Children ′s Hospital of Shenyang from July 2014 to February 2016 were selected,and randomly divided into control group and observation group,46 cases in each group.The control group were treated with open reduction and plate fixation treatment,observation group were treated with elastic intramedullary nail treatment.The surgical effect and prognosis index and the incidence of postoperative complications of two groups were compared.Results The operation time,the amount of bleeding,hospitalization time,fracture healing time and loading time of the observation group were significantly lower than the control group((63.57±3.12) min vs.(94.72±6.49) min,(52.43±11.35) ml vs.(134.26±23.50) ml,(6.10±1.44) d vs.(15.83±2.61) d,(8.34±1.13) weeks vs.(12.05±1.62) weeks,(10.85±2.71) weeks vs.(16.94±3.25) weeks,t=29.34,21.29,22.14,12.74,9.76,P<0.01).The incidence of complications in the observation group was 6.52%(3/46),significantly lower than the control group(19.54%(9/46),χ2=5.06,P=0.02).Conclusion The elastic intramedullary nail for treatment of fractures of extremities shaft closed preschool children can guarantee good clinical effect,and has little trauma high quality,healing of fracture,less postoperative complications and easy care,is a kind of efficient treatment technology,can be widely developed in clinic.

Objective To observe the evaluation of multi‐tumor marker protein chip in breast cancer with neoadjuvant chemo‐therapy ,and to find high saitivity tumor makers ,then evaluate the clinical significance .Methods Forty health examination persons (control group) and sixty‐four breast cancer patients(experimental group) were accepted with multi‐tumor marker protein chip in‐spection ,then we evaluated the markers of diagnosis .The experimental group patients were accepted four cycles preoperative chem‐otherapy with TAC plan ,and tested multi‐tumor marker protein chip three weeks after therapy .Then we evaluated the clinical sig‐nificance of the markers above‐mentioned .Results The positive rate of CA153 ,CA125 ,CEA and FT was 32% ,27% ,21% and 19% in the experimental group ,it indicated significant change compared with the control group (P<0 .05) .And it indicated signifi‐cant reducing change of CA153 ,CA125 and CEA in the experimental group (P<0 .05) after therapy .The sensitivity would be in‐creased when the four markers were tested combined .Conclusion CA153 ,CA125 ,CEA and FT could be used as assistance diagno‐sis index ;CA153 ,CA125and CEA could keep consistence to the curative effect of neoadjuvant chemotherapy and have clinical value .

Objective To investigate whether there are different effects of electric stimulation of vagus nerve,peripheral nerve(sciatic nerve and trigeminal nerve),and motor cortex on pentylenetetrazol (PTZ)induced convulsion in rats.Methods The vagus nerve and sciatic nerve were exposed in rats.The stimulation electrodes were placed on the vagus nerve,sciatic nerve,trigeminal nerve,and motor cortex,respectively.After electric stimulation,PTZ(50 mg/kg)was intraperitoneally injected into the rats.The pattern and latency of the convulsion seizure were observed and recorded.Results Racine's grade Ⅰ-Ⅴ grade convulsion seizure Was present in 9 rats(9/10)in the control group after the injection of PTZ.However,this intensity Was reduced to Ⅰ-Ⅲ grade differentially in all the rats by electric stimulation of the vagus nerve(5/10)or peripheral nerve(6/10 and 5/10).Furthermore,in the group of rats stimulated at motor cortex,there Was completely no convulsion.On the other hand,when pathological changes appeared in cortex or hippocampus(i.e.epileptic model was set up by 7 weeks stimulation),the same stimulation of motor cortex was not able to inhibit the convulsion seizure induced by injection of PTZ and all these rats showed Ⅳ-Ⅴ grade seizure(10/10).Conclusions In physiological condition,all of the four types of stimulation differentially reduced intensity of convulsion seizure triggered by PIZ injection and motor cortex stimulation has the best effect.However.when rats were in pathological status and epileptic nidus appeared in their brains.stimulatiion of motor cortex has no effect on PTZ induced convulsion seizure.

Transrectal ultrasonography is one of the early diagnostic approaches of prostate cancer.In this article,the recent study progression of conventional transrectal ultrasonography with combination of new techniques,such as contrast-enhanced ultrasonography,elastography,image fusion,and so on,in diagnosis of prostate cancer was summarized on the basis of a review of the literatures.

BACKGROUND:Col um Femoris Preserving prosthesis is developed by Pipino, an Italian surgeon, and LINK (Germany). Whether the femoral osteotomy plane recommended in the surgical manual is suitable for Chinese people and whether the osteotomy method can cause stress changes of the prosthesis and femur has not been yet reported.
OBJECTIVE:Based on clinical reviews and three-dimensional finite element model, to analyze the clinical efficacy of total hip arthroplasty with Col um Femoris Preserving prosthesis and relevant stress distribution.
METHODS:We retrospectively analyzed 36 patients undergoing total hip arthroplasty with neck-retaining femoral prosthesis and 36 patients receiving total hip arthroplasty with biotype prosthesis in terms of Harris scores, visual analog scale scores, preoperative and postoperative measurements of eccentricity changes, IDES-Engh radiological evaluation and the incidence of complications. Two different osteotomy plane finite element analysis models were created to analyze the eccentricity difference and changes in the stress distribution of prosthesis and femur when the osteotomy plane was set 1.5 cm above the intertrochanteric fossa or at the junction of femoral head and neck.
RESULTS AND CONCLUSION:Neck-retaining total hip arthroplasty with the osteotomy plane set at the junction between the head and neck achieved good short-term outcomes, showing no difference from total hip arthroplasty with normal biological prosthesis. The finite element analysis showed that the eccentricity increased and the stress on the prosthesis and femur also increased when the osteotomy plane was set at the junction of femoral head and neck compared with that set 1.5 cm above the intertrochanteric fossa. But there was no difference in stress distribution between two osteotomy planes.

Object To evaluate the antioxidative activity of the high molecular weight polyphenols from Sargassum thunbeergii Kuntze and Sargassum kjellmanianum Yendo. Methods Three kinds of evaluation systems for antioxidative activity were adopted to test their efficiency to scavenge radicals (including hydroxyl free radical ?OH, superoxide redical O? 2 and DPPH? free radical). Results The high molecular weight polyphenols from the two kinds of brown algae had strong activity to scavenge the radicals above. Furthermore, they acted almost as the same level. Conclusion The high molecular weight polyphenols from the two kinds of brown algae had strong antioxidative activity, thus they are the potential, natural and marine antioxidants.