Objective To compare the treatment effect of calcium hydroxide and formocresol applied in root canal disinfection after root canal preparation.Methods 283 patients with 388 teeth diagnosed as chronic periapical were selected.Calcium hydroxide and formocresol were applied after opening of pulp chamber,pulp removed and root canal preparation.Calcium hydroxide was piped into the root canal by screw conveyor to fill the root canal in calcium hydroxide group,and formocresol cotton twist was setted in the root canal after dried in formocresol group.Tampons and ZOE were used for temporary closure.Treatment effects in both groups were compared in a week.Results The total effective rate in calcium hydroxide group was 96.39％,while 79.90％ in formocresol group.The total effective rote in calcium hydroxide group was higher than that in formocresol group(x2 =53.792,P ＜ 0.05).Conclusion Calcium hydroxide was safe,periapical tissue irritation and reliable effect was reached.It was worthy of clinical application.

Objection:To construct and characterize TNFR1-GFP fusion protein expression plasmid(pEGFP-TNFR1) and provide a direct and simplified method for assessment of the effect of TNFR1(siRNA) on the TNFR1 gene expression. Methods:Total RNA was extracted from mouse liver.TNFR1 cDNA was amplified by RT-PCR technique and cloned into pMD18-T vector and the sequence was ensured by sequenciog assay.TNFR1 gene was released from pMD-TNFR1 and subcloned into pEGFP-N2 upstream of GFP gene.pEGFP-TNFR1 was analyzed by restrictive enzymatic digestion to ensure the orientation.The plasmid was then transfected into CHO cells,then the fusion protein expression was detected by immunohistochemistry and fluorescent microscope.Rusults:A 1360 bp gene fragment was obtained and coloned into pMD18-T vector,and the sequence was correct.TNFR1 gene was subcloned into pEGFP-N2 vector,and then restriction endonucleases assays showed the correct orientation.24-48 hours after transfection in CHO cells,the expression of TNFR1-GFP fusion protein can be detected by immunohistochemistry and fluorescent microscope.Conclusion:pEGFP-TNFR1 has been constructed successfully.The TNFR1-GFP fusion proteinwas expressed in CHO cells after transtection.It may provide a direct and simplified method for primary assessment of the effect of TNFR1 siRNA on the(TNFR1) gene expression.

This Paper reports the result of the differential Vi HAI test for typhoid carriers inXuzhou.One of 298 normal persons gave a false positive result.The traditional Vi HA I testfor 148 persons in the same group.6 revealed false positive.In 34 typhoid patients,thedifferential HAI test gave a titer of 1:12 to 1:64 in 16 cases(47%)Where as 10 of 239(4.1%)typhoid convalescents had the same titers.

Objective By measuring the concentrations of serum IL-2,IL-4,IL-8 and TNF-αin chronic bronchitis patients before and after the acupoint sticking therapy which was also called winter disease treated in summer to investigate the effect of therapy on serum concentrations of IL-2,IL-4,IL-8 and TNF-α.Methods 30 cases of patients diagnosed with chronic bronchitis in paracmasia and did not recieve other related treatment were randomly divided into acupoint application therapy group (treatment group)and the control group.The treatment group was giventhree Fuacupoint sticking therapy,the control group was given thecomfortacupoint sticking therapy.Serum IL-2,IL-4,IL-8 and TNF-αconcentrations were compared between the 2 groups before and after treatment.Results In the treatment group,the serum IL-2 concentration of patients after treatment was significantly higher than that before treatment(P <0.05),IL-8 and TNF-αconcentrations were significantly lower than those before therapy(P <0.05).Af-ter treatment,serum IL-2,IL-4 levels were significantly higher than those in the control group(P <0.05 )while IL-8 and TNF-αwere significantly lower than those of the control group(P <0.05 ).Conclusion Acupoint sticking therapy in summer for winter diseases effectively increase the concentration of serum IL-2 in patients with chronic bronchitis,reduced IL-8,TNF-α concentra-tions,and enhance their own immunity ability,reduce the degree of inflammation.

Objective:To study Survivin expression in oral squamous cell carcinoma(OSCC) and the effects of pingyangmycin treatment on Survivin expression. Methods:Expression of Survivin was analyzed by flow cytometry and fluorescent indirect mark monoclonal antibody technique in 22 cases of OSCC before chemotherapy,22 cases of OSCC after chemotherapy with pingyangmycin and 8 cases of normal oral mucosa.The Survivin expression was quantified as fluorescence index(FI).Results:The FI in normal oral mucosa,OSCC before chemotherapy and OSCC after chemotherapy with pingyangmycin was 1.0?0.05,1.41?0.06 and 1.22?0.14 respectively(P

Some published research reports on phthalate esters (PAEs) in the municipal sewage sludge were reviewed in the present paper, and aimed at the problems in the field, some preventive measures and the related research emphases were put forwarded also.

OBJECTIVE:To explore the orientation of drugstore clerk’s work role.METHODS:Marketing Grid theory was put forward through analyzing the present wrong-position of drugstore clerk.RESULTS&CONCLUSIONS:Drugstore clerk should locate their right role in work and try to avoid wrong position,and they should take“Shopping Guide”clerk as their chief orientation.

OBJECTIVE: To provide references for the retail pharmacies on market competition. METHODS: The customer satisfactory idea and the customer delivered value theory were analyzed and the ways to enhance the customer delivered value were discussed. RESULTS & CONCLUSIONS: Decreasing drug price is just one among the multi-methods to enhance the pharmacy customer satisfaction. The pharmacies should analyze multi-factors influencing the customer delivered value before making correct marketing strategies.

Objective To explore the effect of case teaching method based on ADDIE model in the teaching of surgical nursing. Methods Two classes of the four-year nursing specialty college students (39 students in each class, 78 students in total) in the third grade were selected as samples. According to the principle of randomization, one class was set as the observation group, and the ADDIE model based on case teaching method was adopted. According to the five stages of ADDIE model, namely, analysis, design, development, implementation, and evaluation, the whole process was performed to achieve teaching goals and timely combination of clinical cases, so that the theoretical knowledge of teaching materials and clinical reality were highly connected. The other class was set as the control group. At the end of the semester, the teaching results were evaluated based on two groups of students' test scores and questionnaire findings. SPSS13.0 software was used for statistical analysis. t-test was used to test the significance of the test results,and chi square test was used to test the significance of the survey data. The results of the two teaching methods were compared with the control group. Results The scores of the two groups were significantly higher than those of the control group [(87.0±7.1) vs (78.0±8.6), P=0.000; (93.0±2.4) vs (85.0±3.8), P=0.000]. Significantly more students in the observation group (P<0.05) were satisfied with the teaching methods used to improve the enthusiasm and initiative of learning, improve their interest in learning, broaden their knowledge, improve the effect and efficiency of learning, inspire students to think, associate, and innovate, activate classroom atmosphere, improve the ability of students to analyze and solve problems, apply theories in practice, and accurately grasp the basic concepts and theoretical knowledge. Conclusion The case teaching method based on ADDIE model can effectively improve the teaching quality of nursing courses and improve students' ability to analyze and solve problems. It is well received by students, and it can enhance teachers' teaching skills as well.

OBJECTIVE:To promote the effective development of hospital pharmaceutical care in China.METHODS:To analyse the disciplines closely related to hospital pharmaceutical care separately.RESULTS&CONCLUSION:Multidisci?plinary support is the basic feature of hospital pharmaceutical care.The cooperation among various technical persons is a deci?sive factor in effective development of hospital pharmaceutical care.