1.DNA methylation in thyroid carcinoma.
Xianyun SONG ; Xiaoling SHANG ; Yutuo ZHANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(6):573-576
Cancer has become clear that not merely gene variations but also epigenetic modifications may contribute to it. Epigenetic changes refer to stable alterations in gene expression with unrelated to changes in the underlying genetic sequence,resulting in heritable. DNA methylation is one of the common epigenetic changes. It control the gene expression through changing DNA conformation and stability, chromatin structer, DNA-protein interaction. The reversal of dysregulated DNA methylation has emerged as a potential strategy for the treatment of thyroid carcinoma. The artical will provide an overview of how DNA methylation contribute to thyroid carcinoma dissemination,invasion and metastasis and we will summarize the latest epigenetic therapies for thyroid carcinoma.
Chromatin
;
DNA Methylation
;
Epigenesis, Genetic
;
Gene Expression
;
Humans
;
Neoplasm Metastasis
;
Thyroid Neoplasms
;
genetics
2.Progress in diagnosis and treatment of children allergic rhinitis.
Yunchao XIN ; Yutuo ZHANG ; Yantao LIN
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(5):400-403
Children allergic rhinitis, referred to as children allergic rhinitis (AR), is a kind of non-infectious inflammation of the nasal mucosa mediated by IgE with the main symtoms of paroxysmal sneezing, rhinorrhoea, nasal itching and nasal obstruction when the susceptible individuals contact the allergen. It is a high reaction disease of the respiratory mucosa common with childhood, which has serious implications to the Children's quality of life, study, rest and growth. The global sampling survey reveals that the morbidity is about 14%, of which 10% in our country and there is an upward trend year by year. At present, drug therapy is still one of the most important methods for children AR. Definite diagnosis, standardized drug therapy and the development of new specific immune therapy make children AR in a good control . This review updates the diagnosis and treatment for children AR, referring to the newest guide by WHO about allergic rhinitis and its impact on asthma (ARIA).
Asthma
;
Child
;
Humans
;
Nasal Mucosa
;
physiopathology
;
Quality of Life
;
Rhinitis, Allergic
;
diagnosis
;
therapy
3.Role of haemophilus influenza factor in the process of biofilm formation
Xue GAO ; Xiaoling SHANG ; Yutuo ZHANG
Tianjin Medical Journal 2015;(3):330-332,333
The Haemophilus influenzae (Hi) is a common opportunistic pathogen from human respiratory tract. The lower immunity can cause the disease. After the biofilm (BF) is formatted by Hi at the site of infection, the antibiotic sensitivity is declined, which often causes the chronic disease, inducing the difficulty in clinical treatment. In recent years, the pathogenic?ity of Hi BF and BF form related factors have gradually become the focus of clinical and basic research. This paper reviewed recent studies as following.
4.Biofilms produced by Haemophilus influenzae in vitro and antibiotics sensitivity changes
Xue GAO ; Xiaoling SHANG ; Haixia QIAO ; Yanxia ZHANG ; Cunhui ZHANG ; Yutuo ZHANG
Journal of Clinical Pediatrics 2014;(7):682-685
Objective To establish a bioiflm (BF) models of Haemophilus inlfuenza in vitro, and to observe the changes of antibiotic susceptibility after the BF fromation. Methods Thirty strains Haemophilus inlfuenzae isolated from adenoids of children with adenoidal hypertrophy and cultured in a 96-well plate. The BF was identiifed by crystal violet staining and scanning electron microscopy (SEM). The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and the minimum bioiflm bacteria bioiflm clear concentration (MBEC) of ampicillin (AMP), ceftriaxone (CRO), levolfoxacin (LVFX) and azithromycin (AZM) were individually detected. Result All of 30 strains of Haemophilus inlfuenzae formed various BF. After BF is formed, the increase of MBEC for different antibiotics was inconsistent with the increase of MIC and MBC. The difference was statistically signiifcant (MBEC/MBC, H=91.54;MBEC/MIC, H=87.91;all P<0.001). The MBEC of AMP was the highest, up to 100 times than the MBC and MIC. The MBEC of CRO was dozens of times than the MBC and MIC. The MBEC of LVFX and AZM were most close to those of MBC and MIC. Conclusion After the formation of BF, resistance to antibiotics of Haemophilus inlfuenzae is enhaced. LVFX and AZM showed more favorable effect on Haemophilus infuenzae BF.
5.Effect of ambroxol on biofilm of Haemophilus influenzae and bactericidal action.
Xue GAO ; Yutuo ZHANG ; Yantao LIN ; Haifeng LI ; Yunchao XIN ; Xiaolei ZHANG ; Yunpeng XU ; Xiaoling SHANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(10):721-723
OBJECTIVE:
To establish a biofilm model of Haemophilus influenzae and observe the effect of ambroxol on biofilm of Haemophilus influenzae and bactericidal action.
METHOD:
Thirty strains of Haemophilus influenzae were isolated from adenoids of children with adenoidal hypertrophy. Two strains which could build stronger biofilms was selected in a 96-well plate. The effect of ambroxol on biofilms were determined by crystal violet, and the structure of biofilms were observed by scanning electron microscope (SEM). The numbers of viable bacterial in biofilm after ambroxol treatmented determined by plate culture count.
RESULT:
Through crystal violet assay, significant difference (P < 0.01) between the two group after treatment was found when ambroxol concentration reached at 0.25 mg/ml and 0.49 mg/ml. The biofilms was destroyed by SEM. Ambroxol had the positive effect on bacterial killing by plate culture count,and the effect was in a dose dependent.
CONCLUSION
Ambroxol could destroy the biofilm of Haemophilus influenzae, and had bactericidal function in vitro.
Ambroxol
;
pharmacology
;
Biofilms
;
drug effects
;
Child
;
Child, Preschool
;
Haemophilus influenzae
;
drug effects
;
Humans
;
Microbial Sensitivity Tests
6.Varieties textual research on "Bangjian": traditional Tibetan medicine including blue, black and variegated flowers.
Lin FU ; Rui GU ; Yu-Ying MA ; Yong-Zhong ZEWENG ; Si-Lang JIANGYONG ; Jing-Bo ZHANG
China Journal of Chinese Materia Medica 2018;43(16):3404-3411
"Bangjian" were traditional Tibetan medicine-flowers from Gentianaceae, which were widely used and had a long medicinal history for the function of detoxifying, curing heat symptoms and treating the laryngitis. The Tibetan compound preparation endowed SFDA approval number always used Bangjian aas the main raw materials for relieving cough, asthma and treating respiratory diseases such as acute and chronic bronchitis. Its commodity medicinal materials were also sold in Qinghai, Sichuan, Tibet and other local medicinal materials market and local specialty marke. However, when recorded by literatures of Tibetan medicine, Bangjian were often classified into white, blue and black or white, blue and variegated according to color of flowers, leading to disordered varieties. In this paper, different Bangjian including their original plants and the main application varieties were studied and authenticated by textual research, wild specimen collection, investigation and collection of samples from Tibetan hospitals,Tibetan pharmaceutical factories and medical material markets. Results showed that Bangjian-including blue, black and variegated flowers were originated from 14 species and 3 varietas according to literatures, and the main application varieties mainly come from Ser. Ornatae of Sect. Monopodiae, such as Gentiana veitchiorum for the most, G. sino-ornata as well as G. lawrencei var. farreri. Suggestion about establishing the quality standard of Bangjian was gived, which provided reference in reasonable use and scientific research for Bangjian, and also had practical value for its clinical use and development.
7. Cancer-associated-fibroblasts regulate the chemoresistance of lung cancer cell line A549 via SDF-1 secretion
Fang ZOU ; Zhihua ZHANG ; Yutuo ZHANG ; Jianqing ZHAO ; Xiulong ZHANG ; Cuiling WEN ; Xianyun SONG ; Waimin ZHOU
Chinese Journal of Oncology 2017;39(5):339-343
Objective:
To investigate whether cancer-associated- fibroblasts (CAF), the key component of tumor microenvironment, regulate the chemoresistant capacity of lung cancer cell line A549 through SDF-1 secretion.
Methods:
Primary cell isolation techniques was used to isolate cancer-associated-fibroblasts from lung cancer patients. MTT assay was applied to determine the proliferation and chemoresistance of A549 cells. Quantative PCR was used to detect the mRNA changes of Bcl-xL. Western blotting was used to detect the protein expression of Bcl-xL. ELISA was applied to detect the SDF-1 secretion from normal fibroblasts (NF) and CAF.
Results:
CAF promoted the proliferation of A549 cells, while NF had no significant effect on them. After 72 hrs incubation, the absorbance value of A549+ CAF medium group was 0.814±0.006, significantly different from the 0.753±0.006 of the A549+ NF medium group (