The paper tries to seek a deeper understanding of the conflicts between doctors and patients based on the review of doctor-patient relationship, and traces the cause of the conflicts from the prospective of basic needs of human beings. It explores the way to construct a harmonious doctor-patient relationship based on the needs of doctors and patients, and suggests that the needs of doctors and patients should be concerned about and guided to meet the developing of doctor-patient harmony.

For the purpose of creating a hospital culture, the present paper summarizes theories on research-oriented organizations, addresses the significance of developing a research-oriented hospital, summarizes the means and methods for developing a research-oriented hospital according to the model of a research-oriented organization, and demonstrates the effects of a research-oriented hospital and promoting functions for hospital culture.

Description of the significance of the team learning theory in building a learning hospital.Building a flat structure in a learning organization, catering to the inherent needs of the hospital and its staff in development, selection of training materials conductive to building common vision of the team, effective and interactive means of training, and team trainings that are objective-specific, level-specific, hatched and focused.All these practices maximize the effectiveness of team learning, bringing forth in the hospital desirable changes in concepts, behavior, culture and performance in the building of a learning hospital

The authors first expound that Chinas WTO accession has made it extremely clear that hospital management is the key to competition. There are two aspects of the issue. One involves changing management concepts, enriching management connotations, broadening the management horizon, enhancing management levels, transforming management mentality, and readjusting management structure. The other involves the application of modern management technology. Then they discuss ways of changing hospital management from the perspective of the competition for the masses of people seeking medical service. These include: ①attracting patients with easily available service; ②heavily advertising the hospital with specific images; ③displaying quality with standardized management; ④winning the confidence of the people with reasonable pricing. Finally the authors give a detailed account of how to explore human resources, retain talented people, form optimal teams, set up rational structures and raise assessment criteria in the competition for medical talents.

Objective To probe into characteristics of medical assurance for medical service preparation, medical staff deployment and injury treatment/rescue for martial arts competitions in Beijing Olympic Games. Methods Collecting data of all injuries treated onsite of martial art venues and in clinics of such venues, for statistical analysis of the injury occasions, injury location, injury types and post-injury treatment. Results During the test games, injuries found in martial arts repertoire competitions account for 0. 92% of all athletes in the category, mostly waist and lower limbs injury; injuries found in sanshou competitions account for 12.4% of all athletes in the category, mostly blunt trauma on head/face, body, perineum and four limbs, along with skin lacerations and 16 heavily wounded transferred to hospitals for rescue. No injuries are found in official games of martial arts repertoire competitions; in sanshou competitions, injuries account for 5. 5% of all athletes of the category, without heavy injuries for hospital rescue. Conclusion Athletes tend to be exposed to greater risk of injury in sanshou than in repertoire competition, headcount of injuries and injury heaviness in test games are higher than official games, probability of injuries of athletes of middle level is higher than those of other levels. Medical staff of professional surgeons of multidisciplinary skills are recommended.

The hospital spirit is not only the spiritual support and vital source for staff, but also the concentrat-ed expression of ideology and faith, value orientation, moral trait, psychology tendencies, culture stereotypes, the unified will and code of conduct for all kinds of personnel.The party organizations are the best helmsmen to culti-vate and carry forward the hospital spirit, which reveals distinctive and profound medical ethical principles and norms.In this paper, taking X hospital as an example, we describe the core function of the party committee′s lead-ership and results in the process of breeding, inheritance and innovation of hospital spirit and reflect ethically so as to provide good references for the current hospital spirit cultivation and promotion.

Spinal cord injury has changed the function of human body and the way of life, and reduced the reserve capacity of the organs of various systems, which exceeded the body's repair ability itself, and overlapped with physiological aging and premature senescence appeared. This paper reviewed recent studies and analyzed the relation of the injured age, the chronological age, number of years after injury with dysfunction, predicted the function changes of them, and identified the factors related with their quality of life.

The paper reviewed the significance and methodology of building a hospital emergency response system, and recommended on coordinating hospital emergency response work. The practices to build emergency response norms include persistent improvement of hospital emergency response system,unifying hospital emergency pre-plan, scheduling emergency drills, training and assessment, performance appraisal, and quantified indicators. The paper also summarized such operation principles as system development, unified management, job division, standardized operation, and routine operations.

A description of the contents, indexes, and method appropriateness for supervision and appraisal of the tobacco control management in hospitals, and an insight on the regularity and effectiveness of building tobacco-free hospitals. The dual indexes of intervention and outcome were ascertained from both dimensions of the management process and effect; in terms of methodology, the paper described the time appropriateness of the supervision focus, management effectiveness of the supervision and appraisal areas, priority of supervision objects, population appropriateness of the supervision and appraisal indexes, method appropriateness of the supervision, appraisal and penalty, as well as the conditional support for supervision and appraisal. It is pointed out that timely summary and appraisal, proper conditional support and circular work chain, can help consolidate tobacco control in hospitals.

Objective To explore the feasibility of using irreversible electroporation (IRE)mediating HPV16 E6 shRNA into cervical cancer cell line SiHa,and to clarify the influence of their co-effect on the proliferation of SiHa cells and its mechanism.Methods A HPV16 E6 gene specific interference sequence was inserted in pGenesil-1 to build a interference vector.10 pulses of IRE with 800 V,100 μs,and 1 Hz were applied to the suspension of SiHa cells and vectors.According to the treatment factors,control group,IRE group,pGenesil-N group,pGenesil-N+IRE group,pGenesil-E6 group and pGenesil-E6 + IRE group were set up.The expression of green fluorescent protein (GFP)and transfection efficiency were confirmed by inverted fluorescence microscope 24 h after the vector was transfected by IRE,and the expression efficancy of GFP was calculated.The expression levels of E6 mRNA and protein were detected by RT-PCR and Western blotting method which was also applied to detect the expressions of P53 and PCNA.The proliferative activity of SiHa cells was determined by CCK-8 assay.Results Enzyme digestion and DNA sequencing verified that the vectors were correctly constructed.GFP was seen under inverted fluorescence microscope 24 h after IRE transfection.Compared with IRE group,the expression levels of E6 mRNA and protein were decreased detected by RT-PCR and Western blotting method after the vectors were treated with IRE,the P53 protein expression level was increased (P < 0.05),and the PCNA expression level was decreased (P <0.05).The CCK-8 assay results showed the proliferative activity of SiHa cells in pGenesil-E6+IRE group was decreased more obviously than that in pGenesil E6 group (P <0.05).Conclusion IRE can play the role of gene transfection of mediating HPV16 E6 shRNA into SiHa cells, and their co-effect can significantly inhibit the proliferation of SiHa cells.