A computer-assisted convolution curve method has been developed based on Glenn's orthogonal function treatment for the determination of multicomponents in mixtures. Once data collection has been made, the computer quickly provides the final answers concerning the concentration of each component togather with statistical information.The inherent disadvantges of Glenn's treatment,including the strictness of select-parameters, the tedious and time-consuming calculations, and the restriction upon the absorption shape of irrelevant absorption are eliminated The determinations of SMZ and TMP in compound tablets of sinomin were demonstrated with excellent results. The principles of the method and the program for calculation were also briefly outlined.

With the development of ultrasonic imaging technique,the thyroid nodule detection rate is ever increasing.However,there is still no unique characteristic for the identification of benign and malignant lesions in ultrasonogram.On the basis of conventional ultrasound performance of nodules,thyroid imaging reporting and data system for the correct classification of thyroid nodules is conducive to their differential diagnosis.Contrast enhanced ultrasound (CEUS) can show the microvascular status of the lesion,and the benign and malignant thyroid nodules can be analyzed by observing the different enhancement patterns.By evaluating the difference in hardness between different tissues,the ultrasonic elastography (.UE) provides a complementary method for the identification of benign and malignant thyroid nodules.Ultrasound-guided fine-needle aspiration biopsy (US-FNAB) can accurately locate and offer pathological basis for preoperative diagnosis of thyroid nodules.It is helpful to preoperatively diagnose the thyroid cancer by detecting the molecular marker of the biopsy specimens which are difficult to be diagnosed clearly.

Objective: To develop a high performance capillary electrophoresis(HPCE) method for the impurity limit test of raw material of ciprofloxacin. Methods: An optimal method was established by studying on pH value, sampling time and other electrophoretic conditions. Results: The RSD of migration time was 0.23% and of the peak area was 2.49%. The limit of detection was 1.21 ?mol/L. The impurity profile and content was consistent with the dilution of sample concentration. Conclusion: HPCE method proves to be suitable for impurity limit test of raw material of ciprofloxacin. [

Bilingual teaching is very important to the development of medical education and integration with the advance medicine knowledge in the world.Rich teaching experiences have been summarized during these years.This article summarizes the bilingual teaching experience from four parts including the concept of bilingual education,conditions of bilingual education,methods of bilingual education,results of bilingual education.We have also found some problems during teaching and worked out the improvement methods.

The optimum convolution spectra of β-galactosidase and the linear equation of Qj=1.906×10-2＋4.946×10-3 C＋1.909×10-4 C2,r=1.0000 under the best condition were established by the convolution spectrum method.Based on the chromogenic substrate o-nitrophenyl-β-galactoside,a method for the determination ofβ-galactosidase activity in the COS-1 extraction sample which had been transfected transiently expression vector of β-galactosidase was determined by using the convolution curve transformation technology.The convolution spectrum method was compared with the absorption method and the result was satisfactory.The method was simple,convenient,fast and valuable to the biochemical analysis.

BACKGROUND:Celastrol is one of the active components extracted from the traditional Chinese medicine Celastrus orbiculatus characterized by expel ing the wind and promoting blood circulation and relieving swel ing and pain.
OBJECTIVE:To investigate the effects of celastrol on tumor necrosis factor-α(TNF-α) induced proliferation and inflammatory responses in RAW264.7 cel s.
METHODS:In vitro inflammatory cel models induced by TNF-α(0, 1, 10, 100μg/L) were treated with celastrol (0.1, 0.5, 1.0, 2.0μmol/L). Interleukin-1β,-6,-8 and prostaglandin E2 in the models were measured by enzyme-linked immunosorbent assay. Cel survival was determined by cel counting kit-8. The inflammatory mediator nitric oxide secretion was detected by nitrate reductase assay. mRNA expressions of inducible nitric oxide synthase, cyclin D1 and cyclin E1 were detected by real-time polymerase chain reaction technique.
RESULTS AND CONCLUSION:Significantly increased secretion of interleukin-1β,-6,-8, prostaglandin E2 and nitric oxide, cel proliferation, and mRNA expressions of inducible nitric oxide synthase, cyclin D1 and cyclin E1 were observed after the induction of TNF-α(0.1, 10, 100μg/L) compared with the control group (without the induction of TNF-α) (P<0.05);especial y, 10μg/L of TNF-αexhibited the strongest effects. 0.4μmol/L celastrol significantly suppressed TNF-α-induced release of interleukin-1β,-6,-8, prostaglandin E2 and nitric oxide, cel proliferation, and mRNA expressions of inducible nitric oxide synthase, cyclin D1 and cyclin E1 in RAW264.7 cel s (P<0. 05). Our results demonstrate that celastrol can inhibit TNF-α-induced inflammatory response and proliferation in RAW264.7 cel s.

AIM: To establish an identification method of Fritillaria based on near-infrared diffuse reflectance spectroscopy(NIRDRS). METHODS: Seven certified Fritillaria,i.e.,F.przewalskii Matim,F.cirrhosa D.Don,F.unibraacteate Hsiaaoet.K.C.Hsia,F.thunbergii Mig,F.pallidiflora,F.ussuriensis Matin and F.kupehensis Hsiaet K.C.Hsia,and three fake "Fritillaria",i.e.,F.thunbergii Varchekiugensis Hsiaet K.C.Hsia,Tulipa edulis and Iphigeniaindica were dried,grinded and sieved before their NIRDRS spectra were detected.Cluster analysis,convolution transform-visualization-similarity analysis were further employed to(identify) the NIRDRS of the ten herbal medicines. RESULTS: The zero-order NIRDRS spectra of the ten herbal medicines were too similar to be identified respectively.The cluster analysis helped differentiate the three fake "Fritillaria" from seven certified Fritillaria,but the identification between the certified Fritillaria remained dissatisfactory.The convolution transform-visualization-similarity analysis magnified and then quantified the minute differences between the certified Fritillaria,which significantly improved their identification. CONCLUSION: NIRDRS is a direct,non-destructive analytical method of solid samples.Coupled with information treatment techniques,DIRDRS provides a new identification method of crude traditional Chinese medicines.

Objective To observe the clinic efficacy of Shenling Baizhu Pill on patients with irritable bowel syndrome(IBS).Methods Ninety patients with IBS were collected and divided into Chinese patent medicine group and western medicine group by random number method,45 patients in each group,the Chinese patent medicine group received Shenling Baizhu pill group treatment,the western medicine group was gave Smectite Powder treatment.The changes of clinical symptoms,intestinal tract microorganism and serum inflammatory factor of patients were observed and compared between two groups before and after treatment.The clinical symptoms was evaluated by questionnaire, the Lactobacillus,Bifidobacterium,enterobacteria and yeast were determined by cultivation,the levels of IL -10,IL -18 and TNF -αwere detected by enzyme linked immunosorbent assay(ELISA).Results Two groups before treat-ment of anaerobic bacteria in feces reduction,aerobic bacteria increased serum IL -18 and TNF -alpha content, increased,the content of IL -10 decreased.Shenling Baizhu Pill intervention for 8 weeks in patients with intestinal anaerobic bacteria[(B/E 1.07 ±0.49)]and serum IL -10[(43.65 ±9.52)mg/L]content increased,aerobic bacte-ria[(B/E 1.07 ±0.49)],IL -18[(82.06 ±21.07)mg/L]and TNF -alpha[(28.44 ±10.20)mg/L]decreased. The IL -18 decreased the most,was different with the western medicine group,there was a statistical significance(t =9.926,P <0.01).Two groups had no bad reaction.Conclusion Shenling Baizhu Pill can effectively regulates and improves the intestinal micro -environment,promote the growth of intestinal probiotics and remodeling the intestinal function of biological barrier,decrease the levels of proinflammatory factors and rise the anti -inflammatory factors level,which has a good clinic effect in treating IBS.

Objective: To qualitatively identify 5 chlorin metal complexes which had similar structures. Methods: The compounds were identified by the convolution curve transformation technology and computer information process technology. Results: The results were demonstrated by match comparison and three dimensional differential diagram of convolution spectra. Five chlorin metal complexes were identified satisfactorily and 10 nonidentity identification results were acquired. Conclusion: The convolution spectrum method is simple and feasible for the identification of the compounds which have similar structures. [