Objective To establish a new enzymatic method for the determination of serum conjugated bilirubin (CB) by the use of bilirubin oxidase (BOD). Method Only the CB was oxidized by BOD under the conditions of pH 5.5 in the presence of sodium fluoride NaF and N-acetylcysteine (NAC) which could prevent the unconjugated and delta bilirubin oxidized. CB was quantitatively determined from a decrease in the absorbance at 450 nm caused by CB oxidization. Results The intra-assay CVs were 6.52% and 0.33% (n=20) at CB concentrations of 17.65 and 301.49 ?mol/L, respectively. The inter-assay CVs were 9.90% and 2.72% at 31.50 and 184.12 ?mol/L, respectively. The linearity was up to 320 ?mol /L. The optimum concentrations of selective inhibitors were 2.5 mmol/L for NaF and 2.5 mmol/L for NAC. The results of serum CB (Y) determined by this method correlated well with those determined by diazo-dye method (Y=0.839X-7.965, r=0.969). Conclusion The proposed reaction conditions could effectively inhibit the oxidation of unconjugated bilirubin by BOD, and thus increase the specificity of CB assay.

Objective : To investigate the clinical effects of different restoration methods on large area defect of deciduous molars. Methods : A total of 150 deciduous molars were selected and randomly divided into three groups: A, B and C. Group A was repaired with glass ionomer and compound resin, B group was repaired by Hall technique, and C group was repaired with metal preformed crown. The successful rate of restoration in 6 and 12 months was compared between the three groups. Results: There was no significant difference between three groups in A, B and C (P > 0.05) in 6 months; the successful rate of 12 months repair in group B and C was significantly higher than that in group A (P < 0.05). Conclusion The success rate of Hall technique and metal performed crown is higher than that of glass ionomer and composite resin on the repair of large defects of deciduous molars.