OBJECTIVE:To develop the method for the determination of riluzole concentration in blood plasma.METH?ODS:Riluzole was extracted with diethyl ether.The residues were analyzed with a reverse phase HPLC system(Diamonsil TM C 18 column,250mm?4.6mm,5?m),with mobile phase of MeOH-0.03mol/L and NH 4 H 2 PO 4 (80∶20,V/V),UV detection wave length of265nm,flow rate of0.8ml/min.RESULTS:The linear coverage of Riluzole was5～1000ng/ml;the lowest detectable concentration was5ng/ml.The average recoveries of riluzole were99.51%,95.74%and97.12respectively.The within-day and between-day relative standard deviations were1.17%、6.48%(n=5)respectively.CONCLUSION:The met_ hod is sensitive,accurate,simple and reliable,and it can be applied to phamacokinetic studies.

Threatened abortion is a common disease of obstetrics and gynecology and one of the diseases responding specifically to traditional Chinese medicine （TCM）. The China Association of Chinese Medicine organized experts in TCM obstetrics and gynecology， Western medicine obstetrics and gynecology， and pharmacology to deeply discuss the advantages of TCM and integrated Chinese and Western medicine treatment as well as the medication plans for threatened abortion. After discussion， the experts concluded that chromosome， endocrine， and immune abnormalities were the key factors for the occurrence of threatened abortion， and the Qi and blood disorders in thoroughfare and conception vessels were the core pathogenesis. In the treatment of threatened abortion， TCM has advantages in preventing miscarriages， alleviating clinical symptoms and TCM syndromes， relieving anxiety， regulating reproductive endocrine and immune abnormalities， personalized and diversified treatment， enhancing efficiency and reducing toxicity， and preventing the disease before occurrence. The difficulty in diagnosis and treatment of threatened abortion with traditional Chinese and Western medicine lies in identifying the predictors of abortion caused by maternal factors and the treatment of thrombophilia. Recurrent abortion is the breakthrough point of treatment with integrated traditional Chinese and Western medicine. It is urgent to carry out high-quality evidence-based medicine research in the future to improve the modern diagnosis and treatment of threatened abortion with TCM.