Objective To investigate the effect of different degree lipidemia on time-resolved fluoroimmunoassay(TRFIA)for determination of unconjugated estriol(uE3).Methods Mixed serum was prepared by collecting different levels of lipidemia samples which were normal and chylous appearance from male and by mixing with definite value serum of uE3.The levels of uE3 in the sam-ples were measured by TRFIA and the effect of lipidemia on TRFIA for determination of uE3 was evaluated.Results For the ap-pearance of chylous specimens,mild lipidemia increased uE3,mid-or hiper-lipidemia samples reduced uE3 and the effect of both was considerable.Conclusion The chylous lipidemia has variant degree of influence to TRFIA for determination of uE3,then the results effect accuracy of Down′s screening.

Objective To investigate epidemiological characteristics of Salmonella infection in children with infectious diarrhea in Zhuhai City from 2009 to 2014 .Methods Isolation ,culturing ,biochemical identification and serotyping of Salmonella were carried out in strains isolated from stool specimens of 4 395 children with infectious diarrhea in inpatient and outpatient settings in Zhuhai Municipal Maternal and Child Healthcare Hospital from 2009 to 2014 ,and data were statistically analysed .Results A total of 546 strains of Salmonella was isolated(the overall isolation rate was 12 .42% ) and 30 serotypes were identified .Among children with Salmonella infection ,those under 3 years old accounted for 93 .22% .There was no statistically significant difference of isolation rate between male and female children with infectious diarrhea(P<0 .05) .June and July were peak period of Salmonella infection ,and the isolation rate was 16 .40% and 16 .09% respectively .Isolates of Salmonella typhimurium ,Salmonella enteritidis and Salmonella stanley account for 50 .92% ,13 .55% and 10 .26% respectively ,which were dominant serotypes .Conclusion Salmonella is the main pathogenic bacteria in children with infectious diarrhea in Zhuhai area ,and Salmonella typhimurium is the main serotype .The infec‐tion of Salmonella might be correlated with children′s age and climate change .

Objective To explore the differences in mental health self-help status of empty-nesters between urban and rural areas for providing the basis and strategies in mental health self-help and promoting development of the self-help potential.Methods Inner Mongolia Chifeng district,441 cases of urban and rural empty-nesters was valuated with the mental health questionnaire.Results Urban empty-nesters were higher than the ratio of rural empty-nester on the aspects of residential satisfaction,participating in activities,talking about their problems in families,communicating health informations and seeking professional help,x2=8.193,15.930,6.500,13.089,18.857,P ＜ 0.05.Urban empty-nesters were lower than the ratio of rural empty-nester on the aspects of feeling helpless and hiding bad mood,x2=9.930,14.873,P ＜ 0.05.The awareness on mental health knowledge was in low level for both urban and rural areas(17.11％,32/187 and 9.55％,19/199).Conclusions Both urban and rural empty-nesters were in bad conditions of mental health self-help ability,especially for rural empty-nesters.The awareness rate of knowledge on mental health is in highly need.

Objective To explore the relative importance of factors of sleep quality in predicting the anxiety and depression emotion. Methods Sleep quality and emotional condition of 112 patients with coronary heart disease were investigated with Pittsburgh sleep quality index(PSQI), self-rating anxiety scale (SAS)and self-rating depression scale (SDS). The relation between sleep quality and emotional condition was analyzed deeply by dominance regression analysis. Results After controlling the demographic vari-able of the sex,factors of the sleep quality such as sleep efficiency and function in the daytime had predict-ing effect on depression emotion;factors such as function in the daytime, sleep quality, sleep efficiency and sleep time possessed predicting effect on anxiety emotion. Conclusions According to different contribu-tion of various factors of sleep quality to anxiety and depression emotion, we should implement selective medical and nursing intervention during clinical work.

Objective To explore the influence of different relaxation training time on relaxation effect of patients with coronary heart disease. Methods 108 patients with coronary heart disease were randomly divided into the morning group, the night group and the morning-night group, each group adopted morning, night and morning-night relaxation training. The relaxation effect was compared between these groups. Results Significant difference existed in sleep quality, anxiety condition and hypnotic dose of the patients in these groups. The time factor played a significant role in the effect of relaxation. The morning group was different in sleep quality improvement and anxiety condition alleviation from the night group and the morning-night group. Conclusions Relaxation training at night or morning-night could effec-tively help improve the sleep quality of patients, decreasing the level of anxiety and tendency of hypnotic in pa-tients with coronary heart disease.

Objective To investigate the correlation between the erythrocyte indices and the genotypes of alpha thalassemia.Methods337 carriers with various genotypes of alpha-thalassaemia ( iron deficiency,alpha-thalassemia double heterozygote and homozygote,α-compounding β-thalassemia and abnormal hemoglobinopathy were excluded) were classified into three groups based on different genotypes of alpha-thalassaemia including silent thalassemia group (ST,83 cases),α-thalassemia trait group (TT,210cases) and intermediate thalassemia group( IT,44 cases),and 154 healthy adults were randomly choosed as normal control The erythrocyte indices involving in RBC,Hb,MCV,MCH,MCHC and RDW-CV were retrospectively analyzed and the difference of which was compared by analysis of variance and SNK test among aboved-mentioned groups.ResultsThere were statistical significance among groups about erythrocyte indices except Hb F.The order of the level of MCV and MCH was NC [( 86.6 ± 5.2) fl,( 29.5 ± 2.1 ) pg] ＞ST[(80.1 ±3.3) fl,(26.7±1.3) pg] ＞TT[(68.5 ±3.4) fl,(22.0 ±1.2) pg] ＞IT[(66.6±7.1)fl,(20.0 ±2.2) pg,F =580.67,761.19,P ＜0.05].And the size of RDW-CV was IT(22.3 ±3.4)％ ＞TT (14.9±1.2) ％ ＞ST(13.8±1.6)％ ＞NC(13.2±1.4)％(F=347.25,P＜0.05).In ST group,the value of MCHC of -α3.7/αα subgroup( 335.6 ± 8.0) g/L was higher than that of -α4.2/αα subgroup( 330 ±7.2) g/L and αTα/αα subgroup (328.4 ±9.5) g/L(F=6.07,P ＜0.05).Meanwhile,in IT group,the value of MCV of αTα/--SEA subgroup( 70.1 ± 7.2 ) fl was higher than that of -α3.7/--SEA subgroup ( 63.4 ±5.9) fl and -α4.2/--SEA subgroup ( 64.1 ± 4.0 ) fl ( F =5.55,P ＜ 0.05 ).However,the value of MCHC of αTα/--SEA subgroup( 289.7 ± 21.2 ) g/L was lower than that of other two subgroups [( 306.3 ± 8.4 ),(306.1 ± 8.7) g/L,F =8.72,P ＜0.05].Except Hb A2 and Hb F,there was positive correlation between the number of deleted α-globin gene and that of RBC and RDW-CV ( r =0.318 and 0.580,P ＜0.01 ).Nevertheless,there was negative correlation between the number of deleted α-globin gene and that of the other erythrocyte indices (r =-0.483,-0.827,-0.744 and -0.684,P all ＜0.01 ).ConclusionsThere is close correlation between the degree of anemia and the number of deleted α-globin gene characterized by Hb reduction and RBC increasing.In addition,the anemia degree of non-deletional Hb H disease is severer than that of deletional Hb H,which of Hb H disease with -α4.2/--SEA is severer than that with -α3.7/--SEA.

Objective To understand the psychological health status and related factors in the primary caregivers of schizophrenia patients in Inner Mongolia,in order to provide empirical basis for nursing care.Methods A sample of 264 chronic schizophrenia patients and their primary caregivers,and 260 local normal individuals were selected according to random cluster sampling.The Symptom Check List-90 (SCL-90) and Social Support Rating Scale (SSRS)were used to evaluate the psychological health status.Symptom severity was assessed with the Positive and Negative Syndrome Scale (PANSS).Results The total SCL-90 score of caregivers of patients was higher than that of local normal individuals,especially the scores of somatization,interpersonal sensitivity,depression,anxiety,hostility,and paranoid ideation were higher than those of local normal individuals.Multiple regression analysis showed that caregivers' sex,caregivers' age,duration of illness,positive symptomatology,negative symptomatology and social support were significant predictors of caregivers' psychological health status.Conclusions The primary caregivers of patients with schizophrenia have worse mental health.Health care professionals should pay close attention to psychological health status in the primary caregivers and provide continuous individualized services,and thereby to improve the primary caregiver' psychological health status.

Objective To explore the influence of the hope level and family function on the quality of life of the schizophrenia convalescence patients.Methods One hundred and twenty schizophrenia convalescence patients were investigated with Herth Hope Scale,Family APGAR Index (APGAR) and the Schizophrenia Quality of Life Scale (SQLS) to analyze the influence of the level of hope and family function on their quality of life.Results The mean score of the hope level and family function of the schizophrenia convalescence patients was (30.1 ±7.0) points and (4.5± 1.5) points respectively,they both played a significant role on the effect of the quality of life.Through the simple effect analysis,the quality of life was different in the various hope level under the various family function situation,and the quality of life was also different in the various family function situation under the medium hope level.Conclusions Both hope level and family function have effect on the quality of life of patients.The medical staff should take targeted measures to improve their quality of life effectively.

Objective To examine the relationship between social functioning and psychiatric symptoms,and the mediation effect of self-esteem and emotion between social functioning and psychiatric symptoms among schizophrenia patients of the convalescent stage.Methods The Personal and Social Performance Scale,Brief Psychiatric Rating Scale,Self-Esteem Scale,General Self-Efficacy Scale were used to investigate patients with schizophrenia of the convalescent stage of Chifeng.Results Social functioning for patients with schizophrenia of the convalescent stage could predict psychiatric symptoms directly.Self-esteem and self-efficacy could make a negative prediction for psychiatric symptoms.Self-esteem and self-efficacy played a mediating role between social functioning and psychiatric symptoms.Conclusions Mental health workers should pay close attention to the mediating roles of self-esteem and self-efficacy,and give comprehensive assessment for the patients and provide continuous individualized psychosocial interventions,and thereby to alleviate psychiatric symptoms.

Objective In view of the schizophrenia risk factors during the recovery,to explore a supportive intervention program suitable for the rehabilitative schizophrenic patients.Methods Totally 64 rehabilitative schizophrenic patients were divided into the experimental group and the control group with 32 cases in each group.All the patients received the same treatment with antipsychotic drugs.The patients in the control group received the routine health education.In addition,the patients in the experimental group received supportive intervention for 4 months.The patients were assessed by IBS,APGAR,GSES and SDSS before and after intervention.Results Main effect of group factor and time factor of GSES,SDSS,APGAR and IBS had statistical significances.There were significant interaction in the scores of SDSS,APGAR and IBS.Simple effect analysis demonstrated the scores of GSES,SDSS,APGAR and IBS in the experimental group were higher than those of the control group after the intervention.Except the score of GSES,there was no differences in other scales before and after the intervention of the control group.Conclusions Supportive intervention program for the rehabilitative schizophrenic patients have good practice guidance.It can improve the patients' self-efficacy,family function and social function,also can correct irrational beliefs and to help patients better adapt to society.