The enzyme,as a biocatalyst,can not be surpassed in speeding up the reactions by other common chemical catalysts under the suitable conditions and can catalyze the reaction mildly,simplify the facilities needed,improve the work conditions,and reduce the production costs,etc.Those are its advantages mentioned above.But while the enzyme is extracted outside the cells,the molecular conformation may be changed with decreasing its bioactivity and stability seriously.As one type of physical energy,(ultrasonic) wave with lower intensity and suitable frequency can make the conformation of enzymatic molecular comformation transform positively and the transforming reactions accelerate so as to facilitate the effective constituents of Chinese materia medica and natural products transforming.

Objective To study the chemical constituents in the immature fruits of Momordica charantia. Methods Isolation and purification were carried out by macroporous absorption resin and silica gel, and compounds were identified and elucidated by spectral and chemical methods. Results Ten compounds were obtained from alcohol extract and five of them were determined as germanicyl acetate (Ⅰ), aglycone of momordicoside Ⅰ (Ⅱ), aglycone of momordicoside L (Ⅲ), charantin (Ⅳ), and ?-sitosterol (Ⅴ). Conclusion For all the first time, compound Ⅰ is found in the plants of Momordica L., compound Ⅱ is found as novel natural product in this plant, and compound Ⅲ is found in China.

Objective To study the chemical constituents in the mature fruit of Momordica Charantia. Methods M. charantia was extracted by alcohol and then extracted by chloroform; isolation and purification were carried out by silica gel. Compounds were identified and elucidated by spectral and chemical methods. Results Five compounds were obtained and identified as aglycone of momordicoside F_1 (Ⅰ), 5, 25-stigmastadien-3-ol (Ⅱ), aglycone of momordicoside Ⅰ (Ⅲ), charantin (Ⅳ), and ?-sitosterol (Ⅴ). Conclusion For the first time, compound Ⅰ is found as the novel natural product and compound Ⅱ is found in the fruit of M. charantia in China.

AIM:To develop a high performance liquid chromatography-electrospray ionization-mass spectrometry(HPLC-EMI-MS)analysis approach of Alisma orientalis(Sam.)Juzep.METHODS:A Zorbax SB-C_ 18 column was used under the gradient elution condition with acetonitrile-H_2O.The MS detection was performed on an Agilent ion trap mass spectrometer with an elctrospray ionization source under the positive ion detection mode.RESULTS:Alisol B,alisol B-23-aceate,alisol C-23-aceate,alisol C,16-oxoalisol A and 11-deoxyalisol C were identified by their MS and MS2 fragments.The MS2 fragments of the 6 compounds were completed.CONCLUSION:The total ion current chromatogram of Alisma orientalis(Sam.)Juzep.was more characteristic than of the UV chromatogram.6 bioactive compounds can be simultaneously analyzed by the HPLC-EMI-MS method established in this experiment and it is a good quality control of Alisma orientalis(Sam.)Juzep.

The garlic oil and oil of Oenothera erythrosepala Borb. in Compound Dashuan Capsules were analysized with several kinds of specific qualitative methods. The content of their main components was also determined by GC. These methods showed relatively high sensitivity and accuracy, and could effectively control the product quality.

Objective To investigate the correlation of metabolism disturbance of oxygen free radical(OFR) with endometriosis and endometrioma, to provide an evidence for the diagnosis and treatment. Methods (1) The lipid peroxide (LPO), selenium (Se), superxide dismutase (SOD) and vitamine E (VE) levels were respectively in the vein blood, ovary tissue of the chocolate cyst wall and chocolate cyst fluid in 32 operated patients with endometriosis (endometriosis group). (2) The levels above were determined seperately in 23 operated patients with endometrioma (endometrioma group) and 30 cases of hysteroma (control group). Results (1)The LPO levels in vein blood and ovary tissue of endometriosis group were (6 357?1 251) ?mol/L and (3 735?1 546) nmol/g protein. Which significantly higher than those of control group ( P 0 05). Conclusion The endome triosis and endometrioma is related to the produce or metabolistic inbalance of oxygen free radical, and so to the increasing of lipid peroxide.

Objective To study the chemical constituents in Momordica charantia.Methods The alcohol extract was isolated by macroporous adsorption resin and silica gel,and the compound structures were identified by spectral methods.Results Two couple isomers were isolated,the structure of one couple was identified as 19R-5?,19-epoxycucurbita-6,23,25-trien-3?,19-diol(Ⅰa)and 19S-5?,19-epoxycucurbita-6,23,25-trien-3?,19-diol(Ⅰb);the structure of the other couple was identified as 3-O-glucopyranoside of 5?,19-epoxycucurbita-6,23,25-trien-3-ol(Ⅱa),and 3-O-allopyranoside of 5?,19-epoxycucurbita-6,23,25-trien-3-ol(Ⅱb).Conclusion New compounds Ⅰa,Ⅰb,Ⅱa,and Ⅱb are isolated from M.charantia for the first time.

Objective To study the chemical constituents of the acid hydrolytic products from the stems and leaves of Panax quinquefolium. Methods The stems and leaves of P. quinquefolium were hydrolyzed with acid;Isolation and purification were carried out by various chromatographic techniques,such as silica gel and so on. Compounds were identified and elucidated by spectral and chemical methods. Results Eight compounds were obtained from the acid hydrolytic products in the stems and leaves of P. quinquefolium and identified as 20 (S)-panaxadiol (Ⅰ),20 (S)-protopanaxadiol (Ⅱ),20 (R)-protopanaxadiol (Ⅲ),20 (S)-panaxatriol (Ⅳ),24 (R)-ocotillol (Ⅴ),20 (R)-protopanaxatriol (Ⅵ),20 (R)-dammarane-3?,12?,20,25-tetrol (Ⅶ),and 20 (R)-dammarane-3?,6?,12?,20,25-pentol (Ⅷ). Conclusion Compounds Ⅰ-Ⅷ are the derivates of the aglycones of ginsenosides,isolated from the acid hydrolytic products from the stems and leaves of P. quinquefolium for the first time. Among these compounds,Ⅳ,Ⅶ,and Ⅷ are discoverd from the roots,stems,leaves,fruits,and buds of P. quinquefolium for the first time. Compound Ⅶ is discovered and reported that it is the derivate of the aglycone of protopanoxadiol ginsenoside,with the conspicuous anticancer activity from the fruits of P. ginseng for the first time.