The enzyme,as a biocatalyst,can not be surpassed in speeding up the reactions by other common chemical catalysts under the suitable conditions and can catalyze the reaction mildly,simplify the facilities needed,improve the work conditions,and reduce the production costs,etc.Those are its advantages mentioned above.But while the enzyme is extracted outside the cells,the molecular conformation may be changed with decreasing its bioactivity and stability seriously.As one type of physical energy,(ultrasonic) wave with lower intensity and suitable frequency can make the conformation of enzymatic molecular comformation transform positively and the transforming reactions accelerate so as to facilitate the effective constituents of Chinese materia medica and natural products transforming.

The garlic oil and oil of Oenothera erythrosepala Borb. in Compound Dashuan Capsules were analysized with several kinds of specific qualitative methods. The content of their main components was also determined by GC. These methods showed relatively high sensitivity and accuracy, and could effectively control the product quality.

Objective To study the chemical constituents in the immature fruits of Momordica charantia. Methods Isolation and purification were carried out by macroporous absorption resin and silica gel, and compounds were identified and elucidated by spectral and chemical methods. Results Ten compounds were obtained from alcohol extract and five of them were determined as germanicyl acetate (Ⅰ), aglycone of momordicoside Ⅰ (Ⅱ), aglycone of momordicoside L (Ⅲ), charantin (Ⅳ), and ?-sitosterol (Ⅴ). Conclusion For all the first time, compound Ⅰ is found in the plants of Momordica L., compound Ⅱ is found as novel natural product in this plant, and compound Ⅲ is found in China.

Objective To study the chemical constituents in the mature fruit of Momordica Charantia. Methods M. charantia was extracted by alcohol and then extracted by chloroform; isolation and purification were carried out by silica gel. Compounds were identified and elucidated by spectral and chemical methods. Results Five compounds were obtained and identified as aglycone of momordicoside F_1 (Ⅰ), 5, 25-stigmastadien-3-ol (Ⅱ), aglycone of momordicoside Ⅰ (Ⅲ), charantin (Ⅳ), and ?-sitosterol (Ⅴ). Conclusion For the first time, compound Ⅰ is found as the novel natural product and compound Ⅱ is found in the fruit of M. charantia in China.

AIM:To develop a high performance liquid chromatography-electrospray ionization-mass spectrometry(HPLC-EMI-MS)analysis approach of Alisma orientalis(Sam.)Juzep.METHODS:A Zorbax SB-C_ 18 column was used under the gradient elution condition with acetonitrile-H_2O.The MS detection was performed on an Agilent ion trap mass spectrometer with an elctrospray ionization source under the positive ion detection mode.RESULTS:Alisol B,alisol B-23-aceate,alisol C-23-aceate,alisol C,16-oxoalisol A and 11-deoxyalisol C were identified by their MS and MS2 fragments.The MS2 fragments of the 6 compounds were completed.CONCLUSION:The total ion current chromatogram of Alisma orientalis(Sam.)Juzep.was more characteristic than of the UV chromatogram.6 bioactive compounds can be simultaneously analyzed by the HPLC-EMI-MS method established in this experiment and it is a good quality control of Alisma orientalis(Sam.)Juzep.

Objective To investigate the correlation of metabolism disturbance of oxygen free radical(OFR) with endometriosis and endometrioma, to provide an evidence for the diagnosis and treatment. Methods (1) The lipid peroxide (LPO), selenium (Se), superxide dismutase (SOD) and vitamine E (VE) levels were respectively in the vein blood, ovary tissue of the chocolate cyst wall and chocolate cyst fluid in 32 operated patients with endometriosis (endometriosis group). (2) The levels above were determined seperately in 23 operated patients with endometrioma (endometrioma group) and 30 cases of hysteroma (control group). Results (1)The LPO levels in vein blood and ovary tissue of endometriosis group were (6 357?1 251) ?mol/L and (3 735?1 546) nmol/g protein. Which significantly higher than those of control group ( P 0 05). Conclusion The endome triosis and endometrioma is related to the produce or metabolistic inbalance of oxygen free radical, and so to the increasing of lipid peroxide.

Objective To find antitumor cOnstituents from Tripterygium hypoglaucum.Methods The chloroform extract of T.hypoglaucum was separated by silica gel column chromatography and preparative HPLC.The structures of compounds isolated were identified by spectral analysis and chemical evidence.Results Seven compounds were isolated and identified as rhein ethyl ester(1),chrysophenol(2),physcion(3),emodin(4),wilfordine(5),wilforgine(6),and wilforine(7).The cytotoxic activities of the compounds against cancer cell lines were assayed.Conclusion Compound 1 is a new natural compound with strong activities against human cancer cell lines (A2780 and OVCAR-3).Compounds 2-4 are isolated from this genus plants for the first time.The possible structure-activity relationship among compounds 1-4 shows that the methoxy group or oxyethyl moiety might be responsible for the cytotoxity.

ObjectiveTo optimize the extraction technology of the active component,rosmarinic acid,an ester of caffeic acid and 3,4-dihydroxyphenyllactic acid,in perilla oil meal for the first time by a new homogenizing technology called smashing tissue extraction (STE).MethodsOrthogonal design was used to optimize the extraction condition.The content of rosmarinic acid was quantified from the methanol crude extract with the help of HPLC.ResultsThe optimization of STE process to get rosmarinic acid from the perilla oil meal was the ratio of liquid to solid material at 10∶1 and the power of extraction at 150 V,extracting twice (2 min for each time).ConclusionSTE could be applied to extracting the active ingredients from the oil meals due to its high extraction efficiency.This new homogenizing technology has advantages on saving extraction time,raising extraction efficiency,and maintaining the temperature sensitive constituents.