Matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) are gelatinases of matrix metalloproteinase family,which play a crucial role in the cancer cell growth,differentiation,invasion,migration,the regulation of tumour angiogenesis and immune surveillance because of their ability to degrade extracellular matrix proteins.So they are significantly associated with development and progression of various tumors.In recent years,the inhibitors and drugs against MMP-2 and MMP-9 arouse wide concern.

Objective To investigate the allngeneic bone marrow mesenchymal stem cells (mesenchymal stem cells, MSCs) transplantation on cardiac rehabilitation and cardiac function after acute myocardial infarction (AMI). Method Acute myocardial infarction models were made by ligating the left anterior descending coronary artery of rats. A total of 90 rats were randomly divided into the cell transplantation group and control group, 45 rars in each group. Rats in the cell transplantation group were subdivided into 3 groups according to the administration time of the allogeneic bone marrow mesenchymal stem cells (2 weeks, 4 weeks and 12 weeks), and the control group was subdivided the same as in the cell transplantation group. The success criteria included the anterior my-ocardial wall darked and the Sr-segment elevation. The rat femur bone marrow cells were separated using centrifu-gal method, mononuclear cells were isolated by lymphocyte fluid, and the third generation of mesenchymal stem cells were obtained by washing, culture, passage and marked by 4, 6 diacetyl-2-phenyl Indole (DAPI). The ani-mals' chest was opened again a week later, the stem cell suspension was injected into the border zone of infarct area, and the control group was injected with medium. Pre-operative,2 weeks, 4 weeks and 12 weeks after trans-plantation, cardiac function was tested by ultrasonic, myocardial biopsy was observed by HE conventional staining, cardiac stem cells division were observed by immunofluorescence. All data was analyzed by software SPSS 10.0, Independent-Samples T test and Chi-Square test was used, and P≤0.05 was considered as statistically signif-icant. Results The markered hone marrow mesenchymal stem cells were left in the myocardial cells and differen-tiated to eardiomyocyte-like cells. After 12 weeks, the left ventricular internal diameter of rats in cell transplanta-tion group was about (0.58±0.09) mm(P<0.05). Thickness of myocardial infarction zone was (0.25±0.01) mm (P<0.05),ejection fraction was (67.52±0.61) (P<0.05) and left ventricular fractional shortening was (39.86±3.00) (P<0.05). In the transplantation group, the DAPI marked nucleus presented as blue, and the cytoplasm of myocardial cell presented as green. HE staining showed that, in the cell transplantation group, there was less scar tissue and a large proportion of nuclear, similar to the fetal or newhom myocardium cells. Conclu-sions The homogeneus mesenchymal stem cell could repair myocardial infarction and improve cardiac function af-ter infarction.

Four cases of abdominal vascular lesions were diagnosed with color Doppler flow imaging (CDFI). These cases include aneurysm of portal vein, splenic aneurysm intrahepatic pseudoaneurysm and carvenous trasformation of portal vein, Above cases were misdiagnosed by B-mode ultrasonogram, but well detected by CDFI. Therefore, we consider that CDFI combined with B-mode ultrasonography, is a noninvasive and accurate method for the diagnosis of abdominal vascular lesion and its differential diagnosis with abnormal, but non-vascular, duct or chamber structure.

Objective To evaluate the value of Doppler indices of intrarenal artery (IRA) in clinicobservation in pregnancy induced hypertension(PIH).MethodsForty-six cases of PIH underwent Doppler measurment of PI,RI in IRA,and 186 normotensive pregnant women were measured as control.Results ①There was a larger increase of IRAPI and IRARI in PIH than in normal prgnant(P<0.001),and IRAPI is significantly higher in moderate and severe PIHs than in mild PIH(P<0.001),②IRAPI,IRARI correlate positively with classification of PIH(P<0.001),proteinuria(P<0.001)and MAP(P<0.05),and IRAPI is the best one.Conclusions It is of a certain significance to the clinical observation and treatment of PIH by using color Doppler to examine the indices of IRA in the pregnant women with PIH.

Adrenal medullary hyperplasia (AMH) is a rare cause of secondary hypertension. Herein we analysed clinical manifestation of 4 cases. The clinical and biochemical features of AMH were similar to those of pheochromocytoma. 131 I MIBG scintigram and CT scanning were helpful to make differential diagnosis. Etiology, diagnosis and treatment of AMH were discussed.

Aim To study the effect of montelukast on eosinophilic airway inflammation of asthmatic guinea pigs,and explore possible mechanism of montelukast. Methods Experimental asthma model of guinea pigs was induced by ovalbumin in vivo. The eosinophils in BALF were separated by density gradient centrifugation. The apoptosis of eosinophils was labeled by TdT-mediated dUTP nick end labeling (TUNEL)technique. The content of IL-5 in BALF was detected by enzyme-linked immunosorbent assay (ELISA). The level of ECP in BALF was detected through fluorescence enzyme-labeled assay. Results In BALF of asthmatic guinea pigs model, the number of eosinophils and content of IL-5 and ECP increased obviously, while the apoptosis index of eosinophils was lowered. After treatment with montelukast, the number of eosinophils and content of IL-5 and ECP in BALF of asthmatic guinea pigs decreased significantly, while the apoptosis index of eosinophils was elevated significantly. Conclusion Montelukast can inhibit the eosinophilic airway inflammation of asthmatic guinea pigs. Lowering the levels of IL-5 and ECP in BALF, promoting apoptosis of eosinophils and relieving airway infiltration of eosinophils may be important mechanisms for montelukast to antagonize airway inflammation of asthma.

By analyzing relevant documents of procurement of drugs with target quantity in Shanghai, com-bined with onsite investigations and interviews, this paper summarizes the practice of procurement of drugs with target quantity in Shanghai from six aspects, namely the basic situation of centralized procurement, pilot scope and variety of procurement of drugs with target quantity, business qualification and certification, determination of successful bid-der, drug distribution, and drug payment settlement. This paper argues that Shanghai has successfully implemented the policy of procurement of drugs with target quantity, by scientifically establishing a comprehensive evaluation index of quality, setting up private drug procurement accounts, prepaying drug procurement loans and strengthening plat-form services and regulatory capabilities, in order to effectively guarantee the operation of procurement of drugs with target quantity. From this case study of Shanghai, it can draw the following implications that in order to successfully achieve procurement of drugs with target quantity mechanism, setting up functional departments of unified authority is the most important guarantee, and using technical means and platform building is the basic conditions. Therefore, the setting up of economic and technical bidding should focus on the individual properties of generic drugs, and the pro-moting of tripartite system reform will help to further expand the scope of procurement of drugs with target quantity.