1.Pharmacokinetics of Etimicin in Patients with Respiratory Tract Infections
Yuqin YIN ; Donglin ZHANG ; Keping DENG ; Guili XU ; Jing ZOU
China Pharmacy 1991;0(06):-
OBJECTIVE: To study the pharmacokinetics of infusion Etimicin in the patients with respiratory tract infec- tions. METHODS: Ten patients were infused with 200mg Etimicin and the concentrations of drug in the plasma, urine and sputurn were determined by microbiological method. RESULTS & CONCLUSlON: The peak concentration of plasma was 17.74?g/ml. The concentration of 0.29?g/ml maintained 12h after administration. Pharmacokinetics parameters conformed to two - compartment model. The pharmacokinetics parameters were, T1/2?= 0. 257h, T1/2? = 2. 22h, Vc = 34. 32L, K2l = 2.14h~(-1), K12 = 0. 48h~(-1), K10 = 0.39h~(-1). The cumulative percentage of renal excretion was 21.37% within 12h, Ke =0. 027h~(-1), K = 0. 431h~(-1). The drug concentration in sputum reached the peak of 2.09?g/ml 2h after infusion.
2.Determination of the Concentrations of Ceftriaxone in Blood and Bile of Patients During Perioperative Period
Yuqin YIN ; Liumeng YANG ; Guili XU ; Jing ZOU
China Pharmacy 2001;0(07):-
OBJECTIVE:To study the anti-infective effect of ceftriaxone in perioperative period METHODS:28 cases of cholelithiasis complicating infections who had to undergo laparoscopic cholecystectomy were enrolled in this study Ceftriaxone,1g,im,was injected 1h~1 5h before anesthesia,and blood and bile were sampled before,during and after operation The concentration of ceftriaxone was determined by UV-spectrophotometry RESULTS:The concentrations of ceftriaxone in blood before,during and after operation were(37 59?16 13)?g/ml,(49 68?10 51)?g/ml and (27 09?12 38)?g/ml respectively The drug concentration in blood peaked 2h after drug administration,at this time,the drug concentration of bile was 4 times that of blood (201 77?20 16)?g/ml CONCLUSION:The drug concentration in blood was 49 times as high as MIC,which indicates that ceftriaxone has preventive and therapeutic effects on possible infections during perioperative period
3.Clinical observation on fatty liver treated by wild apricot in Xinjiang Uighur nationality
Xiaojiang GUAN ; Guanglai LI ; Zhongmin ZHANG ; Yuqin ZOU ; Xiaoqing LAN
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(03):-
Objective:To observe the clinic effect of wild apricot in Xinjiang Uighur nationality on fatty liver.Methods:With a western medicine group as the control group,the treatment courses of both two groups were 12 weeks.The trial group was given wild apricot decoction orally,the control group was given vitamin B,C and Glucurolactone tables.Results:Wild apricot exerted a significant effect in lowering the fat and treating the fatty liver without any toxic and side-effects.Conclusion:Wild apricot in Xinjiang Uighur nationality served as a vital treatment to fatty livers,which was worth being further promoted in the clinical therapy.
4.Epidemiologic study of the distribution of urate crystals in patients with gout by foot dual-energy CT
Lindi JIANG ; Yi ZHOU ; Ying SUN ; Yuqin DING ; Jianjun ZHOU ; Mengsu ZENG ; Hejian ZOU
Chinese Journal of Rheumatology 2012;16(8):556-559
Objective To investigate the distribution of urate crystal as well as the relationship bet ween the features of the crystals and the attacks of joint pain and/or swollen by foot dual-energy CT.Methods Eight-four patients (68 were diagnosed as gout, 11 were patients with hyperuricemia and 5 were diagnosed as other types of arthritis) who recently experienced foot swelling and/or pain were enrolled and all of them were performed foot dual-energy CT.The relationship between the features of the urate crystals and the attacks of gouty arthritis was determined by Chi test and the potential risk factors were identified by Logistic multiple regression analysis.Results Two hundred and seventyeight urate crystal depositions were found in 68 gout patients,and the most common deposition sites were the distal parts of the first toe(18.2%),the first metatarso-phalangeal joint ( 16.8% ),calcaneus ( 17.5% ),the lower end of tibia ( 11.8% ).Furthermore,patients with the urate crystals deposited in the first metatrasophalangeal joint or the lower end of of tibia were more likely to experience acute episodes of gout attack (P<0.01,P<0.05 respectively).In addition,the shape,size and quantity of urate crystals also affected episodes of acute attack of gout.Conclusion Dual-energy CT,which is a non-invasive method,could clearly reveal urate crystal depositions and is helpful for the diagnosis and follow-up of patients with gout.The location,shape,size and quantity of urate crystals and soft tissue swelling,bone erosion may affect the acute attack of gout.
5.Molecularmechanism of Bel subtype caused by mutation p.R168W of glycosyltransferase B
Songsong GONG ; Wei ZOU ; Fang LI ; Yuqin WANG ; Hang LEI ; Xuefeng WANG ; Xiaohong CAI
Chinese Journal of Blood Transfusion 2017;30(7):679-681
Objective To study the molecular mechanism of Bel subtype caused by mutation p.R168W of glycosyltransferase B.Methods Serological test,SSP-PCR and direct sequence the Exon6 and Exon 7 of the ABO gene.Construct a 3D molecular model and predict the structural impact of GTB protein mutations.Results A antigen or B antigen can't be detected on the surface of the propositus' RBC,and only anti-A antibodies were detected in her serum.But serological test indicated her daughter's blood type was a normal B type.SSP-PCR test indicated propositus' ABO gene type is O1 B.By gene sequencing the Exon 6 and Exon 7 of the ABO gene,a ABO Bel allel(c.502C>T,p.R168W)was discoverd in both the propositus and her daughter.Through the propositus' daughter coexisted Bel gene with normal B gene,her blood type was a normal B type.Conclusions ABO gene c.502C>T mutations cause Bel phenotypes in patients by reducing the stability of GTB.
6.Rapid screening of a hotspot variant c.609G>A in MMACHC gene by using PCR-high-resolution melting curve analysis.
Shuxiang LIN ; Chao WANG ; Xinjie ZHANG ; Xiaowei XU ; Qianqian ZOU ; Chunquan CAI ; Yuqin ZHANG ; Jianbo SHU
Chinese Journal of Medical Genetics 2020;37(7):759-763
OBJECTIVE:
To carry out genetic testing for two families affected with cobalamin C (cblC) and establish a rapid method for the detection of a hotspot pathogenic variant c.609G>A of the MMACHC gene by using a PCR-high-resolution melting curve (PCR-HRM) method.
METHODS:
Genomic DNA was extracted from peripheral blood samples of the probands and their parents. Potential variants of the MMACHC gene was analyzed by Sanger sequencing. The c.609G>A variant of the MMACHC gene was screened among 100 healthy children with the PCR-HRM method.
RESULTS:
Sanger sequencing revealed that proband 1 carried compound heterozygous variants c.394C>T and c.609G>A of the MMACHC gene, while proband 2 carried compound heterozygous variants c.482G>A and c.609G>A of the same gene. PCR-HRM analysis of the two probands and the 100 healthy children were consistent with the Sanger sequencing.
CONCLUSION
c.609G>A is a hotspot pathogenic variant of the MMACHC gene. The diagnosis of cblC may be rapidly attained through detection by PCR-HRM.
7.Recommendations for prescription review of commonly used anti-seizure medications in treatment of children with epilepsy
Qianqian QIN ; Qian DING ; Xiaoling LIU ; Heping CAI ; Zebin CHEN ; Lina HAO ; Liang HUANG ; Yuntao JIA ; Lingyan JIAN ; Zhong LI ; Hua LIANG ; Maochang LIU ; Qinghong LU ; Xiaolan MO ; Jing MIAO ; Yanli REN ; Huajun SUN ; Yanyan SUN ; Jing XU ; Meixing YAN ; Li YANG ; Shengnan ZHANG ; Shunguo ZHANG ; Xin ZHAO ; Jie DENG ; Fang FANG ; Li GAO ; Hong HAN ; Shaoping HUANG ; Li JIANG ; Baomin LI ; Jianmin LIANG ; Jianxiang LIAO ; Zhisheng LIU ; Rong LUO ; Jing PENG ; Dan SUN ; Hua WANG ; Ye WU ; Jian YANG ; Yuqin ZHANG ; Jianmin ZHONG ; Shuizhen ZHOU ; Liping ZOU ; Yuwu JIANG ; Xiaoling WANG
Chinese Journal of Applied Clinical Pediatrics 2023;38(10):740-748
Anti-seizure medications (ASMs) are the main therapy for epilepsy.There are many kinds of ASMs with complex mechanism of action, so it is difficult for pharmacists to examine prescriptions.This paper put forward some suggestions on the indications, dosage forms/routes of administration, appropriateness of usage and dosage, combined medication and drug interaction, long-term prescription review, individual differences in pathophysiology of children, and drug selection when complicated with common epilepsy, for the reference of doctors and pharmacists.
8. Mechanism of Bmal1 Involved in Irritable Bowel Syndrome via TPH1-5-HT Signaling Pathway in Enterochromaffin Cells
Weiwei ZENG ; Yanjun WANG ; Lu ZOU ; Yuqin HE ; Shili XIAO ; Jing WANG ; Dan QIAO ; Rong ZHAO ; Bin WANG ; Dongfeng CHEN ; Min YANG ; Mei ZHAO ; Shuai WANG
Chinese Journal of Gastroenterology 2022;27(6):321-327
Background: Disrupted circadian rhythms have been associated with the development of irritable bowel syndrome (IBS). In some IBS patients, the symptoms may present with circadian fluctuations. Enterochromaffin cells (EC cells) and tryptophan hydroxylase 1 (TPH1) - 5 - hydroxytryptamine (5 - HT) signaling pathway are currently recognized as the key pathophysiological mechanism of IBS. Aims: To explore whether Bmal1, the core circadian clock gene, is involved in the occurrence of IBS by regulating TPH1-5-HT signaling pathway in EC cells. Methods: Normal Sprague-Dawley (SD) rats and IBS-model SD rats, as well as wild type (WT) and intestine-specific Bmal1 knockout (Bmal1