OBJECTIVE:To observe the efficacy and safety of the effective blood concentration of cyclosporin A in patients after bone marrow transplantation.METHODS:By fluorescence polarization immunoassay(FPIA),the whole blood trough concentration of CsA in 14 patients after undergoing bone marrow transplantation was monitored for 216 times in total from day 1 to day 466,and the monitoring results were analyzed.RESULTS:The effective blood concentrations of CsA in patients with chronic myelocytic leukemia(CML),acute non lymphocytic leukemia(AML),acute lymphoblastic leukemia (ALL),Mediterranean disease(THAL),and pancytopenia were(50～450) ng?mL~(-1),(100～450) ng?mL~(-1),(100～350) ng?mL~(-1)(200～500) ng?mL~(-1),and(250～500) ng?mL~(-1),respectively.Rejection reaction appeared in 7 cases after bone marrow transplantation,with whole blood trough concentration of CsA ranged from 67.4 ng?mL~(-1) to 189.34 ng?mL_(-1) (125.44?39.56 ng?mL~(-1)on average).Adverse drug reaction appeared in 3 cases,with trough concentration of CsA ranged from 412.5 ng?mL~(-1) to 548.62 ng?mL~(-1)(481.39?68.08 ng?mL~(-1) on average).CONCLUSION:Timely monitoring of plasma concentration of CsA and modifying of the dosage regimen can avoid the occurrence of rejection reaction and adverse drug reaction,which is of great significance for patients' medication safety and efficacy.

0.05);the cure rates were 55.77%,36.54% and 88.46%,re-spectively;the recurrence rates were 11.54%,25.00% and 1.92%,respectively.CONCLUSION:Scheme A is the preferable one economically,however,taking all the factors into consideration,scheme C is the best one.

In recent years, according to the etiology and pathology researches of slow transit constipation (STC) STC is considered as a kind of "enteric neuropathy", indicating it is a kind of disease caused by abnormity of the enteric nervous system (ENS). Through reviewing the mechanism of acupuncture to regulate STC, it is found out that there is a close relationship between acupuncture regulating STC and ENS. Through various channels including ganglion cells, nerve plexus, neurotransmitter and TRPV1 (the primary sensory neurons receptor of the ENS) of the ENS, acupuncture is likely to make comprehensive adjustment on STC.
Acupuncture Therapy ; Colon ; innervation ; physiopathology ; Constipation ; physiopathology ; therapy ; Enteric Nervous System ; physiopathology ; Humans

【Objective】To observe the effect of electro-acupuncture(EA) and Banxia Xiexin Decoction(BXD) on motilin levels of functional dyspepsia(FD) rats.【Methods】 Sixty SD rats were randomized into normal control group,model group,mosapride citrate tablets(MCT,2.82 mg?kg-1?d-1) group,EA group,BXD(33g?kg-1?d-1) group,EA + BXD group.Except the normal control group,the rats with FD in other groups were induced by stimulating the rat tails with forceps clip. EA group received EA on bilateral acupoints of Zusanli(ST36) for consecutive 7 days.MTL content in rats plasma,antrum,jejunum,and hypothalamus was observed with radioimmunological method.【Results】MTL content decreased in the plasma,antrum,jejunum,and hypothalamus of the model group(P

OBJECTIVE:To discuss the general rule on checking and acceptance of drug warehousing of prepared slices of Chinese crude drugs.METHODS:The warehousing acceptance data of prepared slices of Chinese crude drugs during 2003-2007 in our hospital were sorted and the reasons for drug acceptance rejection were analyzed.RESULTS:Of the 5 228 batches of prepared slices of Chinese crude drugs checked before acceptance over 5 years,138(2.61%) were unacceptable.Most of the rejected batches were prepared processing slices of Chinese crude drugs.There were more than 30 factors resulted in the rejection.CONCLUSION:The key to ensure the quality of prepared slices of Chinese crude drugs is to improve their processing level.

Objective To incestigate the fetures of cranial CT and MRI in the patients with eclamptic encephalopathy.Methods The CT and MRI findings of eight cases of eclamptic encephalopathy with the charge of CT,MRI appearance of FLAIR(fluid attenvated inversion-recovery),DWI(difussion weighted imaging),ADC(apparent diffusion coefficient)were analyzed retrospectively.Results Of eight patients,5 cases had abnormal finding in the cranial CT with showed symmetric plaque-like low-attenuated lesions in cortex and subcortical white matter of parietal and occipital lobes in six cases;eight cases had abnormal findings in the cranial MRI,the lesions were demonstrated as slightly hypointensity on T1WI and slightly hyperintensity on T2WI and remarkably hyperintensity on FLAIR,and iso or slightly hyperintensity on DWI,and remarkably hyperintensity on ADC.The lilateral parietal occipital lobes and cerebellar hemisphere and Brain Stem were the more common sites.Conclusions The only characteristric findings of eclamptic encephalopathy in MRI and CT imaging studies is vasogenic edema and reversible,especially in the subcortical white matter of the parietal and occipital lobes bilaterally,and cereballar hemisphere et al;especially cranial CT showed symmetric plaque like low-attenuated lesions of posterior brain;FLAIR,DWI and ADC of MRI can be helpful for early diagnosis and diffenential diagnosis,prognosis and curative effect of hypertensive encephalopathy.

This paper introduces the setting and configuration of our hospital's fPACS workstation, which is developed by the First Military Medical University. The fPAX video workstations are used as the terminals, including fPAX sampling workstation for video, picture and number, fPAX diagnosis workstation, fPAX clinician workstation and fPAX consultation center workstation. The workstation is equipped with an IBM4 2G host computer, an 80G fixed disc, an EMS memory more than 512M and a SONY 21″ pure flat display. Our experience of building PACS workstation proves that the reasonable setting and configuration is helpful to save the money and increase the efficiency.

This study was aimed to observe the clinical effect of acupuncture together with warm acupuncture on fe-male type 2 diabetes mellitus (T2DM) patients of dual deficiency of yin and yang syndrome. A total of 92 female T2DM patients of dual deficiency of yin and yang syndrome were divided into two groups, which were priority of yin deficiency in the type of dual deficiency of yin and yang (group 1) and priority of yang deficiency in the type of dual deficiency of yin and yang (group 2). There were 46 cases in each group. The treatment of acupuncture together with warm acupuncture was given for three month. The observation was made on the fasting plasma glucose (FPG), fasting leptin (FLP), fasting insulin (FINS), insulin sensitivity index (ISI), insulin resistance index (Homa-IR), insulin secre-tion index (Homa-β), total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high den-sity lipoprotein cholesterol (HDL-C), body mass, body mass index (BMI) and body fat percentage (F%) before and af-ter treatment. The results showed that the total effective rate of group 1 was 69.57%, while the total effective rate of group 2 was 84.78%. There was no significant statistical difference between two groups. Compared with pretreatment, there were significant decrease on FLP, FPG, FINS, Homa-IR, TC, TG, LDL-C, body mass, BMI, and F% of both groups after treatment (P < 0.01). There was obvious increase on ISI, Homa-β, and HDL-C (P < 0.01). After treat-ment, the FPG, Homa-β and BMI of group 2 were better than group 1 (P< 0.05). After treatment, the level of LDC-C and HDC-L of both groups reached normal level. And the FPG of group 2 also reached normal level. It was con-cluded that acupuncture together with warm acupuncture can significantly adjust the level of fat-pancreatic islet shaft of T2DM patients of dual deficiency of yin and yang syndrome, reduce the leptin resistance and insulin resistance, enhance the sensibility of insulin and the function of pancreatic β cell, and correct the endocrine disorder, gly-cometabolism disorder and lipometabolism disorder. Acupuncture together with warm acupuncture did much better in lowering blood sugar and improving the function of pancreatic β cells of T2DM patients whose priority of yang defi-ciency in the type of dual deficiency of yin and yang than those whose priority of yin deficiency in the type of dual deficiency of yin and yang.

Background:As an important catalytic subunit of telomerase,human telomerase reverse transcriptase (hTERT)plays an important role in the development and progression of many cancers including gastric cancer.It has been reported that several single nucleotide polymorphisms (SNPs)of hTERT had varying degrees of association with risk of neoplasms. Aims:To study the correlation between SNPs of hTERT rs2853676 and rs2853677 and susceptibility to gastric cancer. Methods:Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the genotypes of rs2853676 and rs2853677 of hTERT in 297 gastric cancer patients,105 atrophic gastritis and 402 controls. Helicobacter pylori (Hp)infection was detected by pathological examination and 13 C-urea breath test.Results:Frequency of AA genotype of rs2853676 was significantly higher in gastric cancer group when compared with control group (15.2%vs.6.5%,P =0.01).The risk of gastric cancer in AA genotype carriers increased 2.47-fold (95% CI:1.46-4.16) when compared with GG carriers.No significant differences in the frequencies of CC,TC and TT genotypes of rs2853677 were found among gastric cancer patients,atrophic gastritis patients and controls.Hp infection rates in atrophic gastritis group and gastric cancer group were significantly increased than those in controls (64.8%,56.9% vs.40.3%,P all <0.01),OR were 2.73 (95% CI:1.74-4.26),1.96 (95% CI:1.44-2.67),respectively.Logistic regression analysis showed that there was no significant interaction between Hp infection and gene mutation.Conclusions:Polymorphism of hTERT gene rs2853676 may play a role in susceptibility to gastric cancer,and Hp infection may not be involved in the increase of risk of gastric cancer caused by hTERT gene polymorphism.

Aim To study the effect of 2,3,4′,5-tetrahydroxystilbene-2-O-?-D glucoside(TSG) on vascular smooth muscle cells(VSMCs) proliferation induced by fetal bovine serum and explore the underlying mechanisms.Methods VSMCs were cultured from rat aorta using explant technique.VSMCs proliferation was analyzed by 5-bromo-2-deoxyuridine(Brdu) incorporation assay.Cell cycle phase distributions were determined by flow cytometry.The expression of proliferating cell nuclear antigen(PCNA) was evaluated by Western blot analysis.The level of intracellular reactive oxygen species(ROS) was estimated through fluorescence assay.The intracellular activities of superoxide dismutase(SOD),catalase(CAT),and glutathi one peroxidase(GSH-px) were measured using biochemical method.Results TSG in the experimental concentration 0.1～100 ?mol?L~-1 exhibited no apparent cytotoxocity.10 ?mol?L~-1 and 100 ?mol?L~-1 TSG significantly inhibited the proliferation of VSMCs induced by serum,cell cycle transition from G0/G1phase to S phase,PCNA expression in nucleus of VSMCs and level of intracellular ROS.100 ?mol?L~-1 TSG markedly increased the activities of SOD and GSH-px.Conclusions Certain concentration of TSG can prevent VSMCs proliferation effect mediated by serum;the mechanism about TSG effect may be associated with arresting G0/G1 to S progression,decreasing PCNA expression and improving the antioxidation of VSMCs.