1.Primary Study on Pathogeny and Pathogenesis Caused by Tobacco
Chinese Journal of Information on Traditional Chinese Medicine 2017;24(9):118-119
This article conducted the discussion based on the flavor and meridian tropism, invasive pathways, pathogenesis features, and transmission prognosis of tabacco. It proposed that the lung defence are firstly attacked by tobacco evil qi; then evil qi is accumulating to be toxic; toxin hides in the Taiyin; viscera is weak; qi is exhausted and the body fluidis impaired; toxin victimizes vessel and fumigates the viscera, so all kinds of diseases engender.
2.Membrane oxygen enrichment for medical health care
Yuping SUN ; Xiaolan ZHOU ; Guanglin SHEN
Chinese Medical Equipment Journal 2003;0(11):-
Membrane oxygen enrichment gives advantage over other oxygen source when applied to medical healthcare for being curative,safe,economical,convenient,small,light,durable,easy to operated,reliable and free from side-effect.The products related,for instance membrane oxygen enricher,membrane oxygen-bar air-conditioner,mobile oxygen-bar and so on,are the best sources of oxygen supply for long-term oxygen therapy,healthcare for healthy people and oxygen enriched room at high altitude.
3.inhibits growth and increases radiosensitivity of tumors in nude mice implanted with nasopharygeal carcinoma cells
Huaping XIAO ; Rongrong ZHOU ; Yuping LIAO
Chinese Journal of Cancer Biotherapy 2006;0(06):-
Objective:To study the inhibitory effect of E1A gene on the growth of tumors in nude mice implanted with nasopharygeal carcinoma CNE2 cells and its promotion effect on the radiosensitivity of CNE2-implanted tumors,and to investigate the related mechanism.Methods: E1A gene was transfected into CNE2 cells using adenovirus system,and stable E1A positive clones were established.The inhibitory effect of E1A on tumor formation-ability of CNE2 cells was observed in nude mice.The efficacy of E1A gene therapy with or without radiotherapy against CNE2 cell-implanted tumors was evaluated.The effect of E1A gene therapy on the expression of P53 was detected by RT-PCR.Results: CNE2 cells stably transfected with E1A gene(CNE2-Ad-E1A)were successfully established.The tumor formation time was later and tumor size was smaller in CNE2-Ad-E1A cell-implanted mice compared with those in CNE2 cell-and CNE2-Ad-?-gal cell-implanted mice(CNE2 cells stably transfected with Ad-?-gal).Radiotherapy,E1A gene therapy and E1A gene+radiotherapy all suppressed the growth of implanted tumors,with the tumor suppression rates being(60.32?5.34)%,(70.53?6.12)%,and(97.15?4.87)%,respectively.E1A gene therapy significantly increased the expression of P53 gene in tumor tissues.Conclusion: E1A can inhibit the growth of tumors in mice implanted with nasopharygeal carcinoma cells,and enhance its sensitivity to radiotherapy,which may be related to the increased expression of P53 gene in tumor tissues.
4.Isolation of Pityrosporum ovale from a Patient with Onychomycosis: Clinical and Laboratory Investigation
Yuping RAN ; Guangping ZHOU ; Wei LI
Chinese Journal of Dermatology 1994;0(05):-
Objective To isolate and observe Pityrosporum yeasts from a patient with onychomycosis. Methods The involved nail specimens were investigated by means of culture, pathological and scanning electron microscopic examination and 20% KOH preparation. Results Physical examination showed that each finger and toe nail appeared brownish black, rough and thick, some of the fore part of the nail plate detached from the nail bed. Fingernail specimen's culture results showed that Trichophyton rubrum grew on Sabouraud's dextrose agar and Pityrosporum ovale grew on the medium containing rapeseed oil. The pathological examination revealed P.ovale yeast involvement in the fissure of the nail plate. Under the scanning electron microscopy, a lot of P.ovale yeasts with characteristic collarette structure inserted in the nail tissue was noticed. In the 20% KOH preparations of nail incubated at 56℃for 1h and stained with Quink Parker ink, spores and hyphae were identified morphologically with P.ovale and T.rubrum respectively. The patient received intermittent pulse therapy with itraconazole, the color of the nails became much brighter 1 to 2 months after the fourth cycle of therapy, but no further improvement was observed afterwards. P.ovale and T.rubrum grew again 6 months after treatment when the clippings of the fingernail were cultured. Conclusion This is the first document of onychomycosis related with P.ovale in China.
5.Discussion on Practice Teaching of Pharmacy Speciality Students
Yong CHEN ; Zhou JIANG ; Yuping GUO
Chinese Journal of Medical Education Research 2002;0(01):-
By analyzing the quality requirements for the majors in pharmacy speciality and the shortcomings existing in present practice teaching this article brought up the corresponding improving measures,which include courses and teaching modes innovation,teaching ability improvement and management reinforcement.
6.The clinical results of a new type of phaco tip:MicroTip
Zhende, LIN ; Yuping, ZHOU ; Shaozhen, LI
Chinese Journal of Experimental Ophthalmology 2001;19(1):77-78
ObjectiveTo investigate the methods of reducing eye injury in phacoemulsification.MethodsPhacoemulsification with intraocular lens(IOL)implantation was performed on 98 cases(98 eyes)of cataract using a new type of phaco tip(MicroTip)with high vaccum and low energy.All the nuclei was harder than Grade Ⅳ.Postoperative visual acuity and eye injury were observed,including corneal edema,anterior chamber in flammation,intraocular pressure,IOL position and retinal condition.ResultsPostoperative visual acuity is 0.5 or better in all cases,1.0 ? or better in 28 cases(28.6%).The complication during operation,such as anterior chamber hemorrhage,posterior capsule rupture and vitreous loss,did not occur.Postoperative complications were very slight.Two eyes(2%)suffered mild corneal edema that lasted no more than a week.No other complications were found.ConclusionMicroTip is a kind of safe and effective facility for phacoemusification,with which we can reduce the eye injury,especially dealing with the hard nuclei.
7.Overview of molecular diagnosis and targeted treatment of tumor
Xiaowen ZHENG ; Yuping WANG ; Yongning ZHOU
Journal of International Oncology 2015;(10):756-758
The occurrence and outcome of cancer is related to the involved gene;Detecting the expres-sion of gene in body by the real-time PCR technology can diagnose and evaluate the prognosis of patients early. Through analyzing the abnormal genes and molecular,designing and studying the targeted drugs can achieve the therapeutic effect of steady,accurate,relentless,at the same time to reduce side effects and to improve the quality of life.
8.E1A inhibits growth and increases radiosensitivity of tumors in nude mice implanted with nasopharygeal carcinoma cells
Huaping XIAO ; Rongrong ZHOU ; Yuping LIAO
Chinese Journal of Cancer Biotherapy 2009;16(6):614-618
Objective:To study the inhibitory effect of E1A gene on the growth of tumors in nude mice implanted with nasopharygeal carcinoma CNE2 cells and its promotion effect on the racliosensitivity of CNE2-implanted tumors, and to investigate the related mechanism. Methods: E1A gene was transfected into CNE2 cells using adenovirus system, and sta-ble E1A positive clones were established. The inhibitory effect of E1A on tumor formation-ability of CNE2 cells was ob-served in nude mice. The efficacy of E1A gene therapy with or without radiotherapy against CNE2 cell-implanted tumors was evaluated. The effect of E1A gene therapy on the expression of P53 was detected by RT-PCR. Results: CNE2 cells stably transfected with E1A gene (CNE2-Ad-E1A) were successfully established. The tumor formation time was later and tumor size was smaller in CNE2-Ad-E1A cell-implanted mice compared with those in CNE2 cell- and CNE2-Ad-β-gal cell-implanted mice (CNE2 cells stably transfected with Ad-β-gal). Radiotherapy, E1A gene therapy and E1A gene + radio-therapy all suppressed the growth of implanted tumors, with the tumor suppression rates being (60.32±5.34) %, (70.53±6.12) %, and (97.15±4.87) % , respectively. E1A gene therapy significantly increased the expression of P53 gene in tumor tissues. Conclusion: E1A can inhibit the growth of tumors in mice implanted with nasopharygeal carcinoma cells, and enhance its sensitivity to radiotherapy, which may be related to the increased expression of P53 gene in tumor tissues.
9.Malassezia globosa is the Main Species Found in Hair Follicles of Patients with Malassezia Folliculitis
Xincai XIONG ; Yuping RAN ; Yalin DAI ; Lin XIONG ; Guangping ZHOU
Chinese Journal of Dermatology 2003;0(08):-
Objective To examine the distribution of Malassezia species in follicular contents and perifollicular superficial skin in patients with Malassezia folliculitis and search for its causative agent. Methods A total of 120 patients with Malassezia folliculitis were investigated. Follicular lesions at three different anatomic sites were selected in each patient. Perifolliclar superficial skin specimens were taken by sterile adhesive tape, and the follicular contents of the same follicle were taken by sterile haemostatic forceps. The above specimens were cultured respectively on media containing rapeseed oil. The isolated colonies were identified by their physiological and morphological characteristics. Results Out of 319 isolates obtained from the perifollicular superficial skin, 247 isolates (77.43%) were identified as M. sympodialis, 40 isolates (12.54%) as M. furfur, 27 isolates(8.46%) as M. globosa and 5 isolates(1.57%) as M. obtusa. Out of 314 isolates obtained from follicular contents, 252 isdates(80.25%) were identified as M. globosa, 57 isolates(18.15%) as M. sympodialis, 4 isolates(1.27%) as M. furfur, and 1 isolate(0.32%) as M. obtusa. There was statistical difference in species distribution between the follicular contents and the perifolliclar superficial skin (P
10.Effect of E1A gene on radiosensitivity of human laryngeal carcinoma cells and its correlated mechanisms
Yuping LIAO ; Sijuan DING ; Rongrong ZHOU ; Huaping XIAO
Chinese Journal of Radiation Oncology 2008;17(6):467-469
Objective To investigate the effect of El A gene on the radiosensitivity of human laryngeal carcinoma cells and its correlated mechanisms. Methods The Ad-E1A and Ad-β-gal were amplifieated in Hek293 cells, extracted by freezing (-80℃) and thawing(37℃) repeatedly (3 times) , purificated by the method of density gradient of CsC1 and titrated by plaque assay method. Then they were transfected into human laryngeal carcinoma cells (Hep-2) and authenticated by RT-PCR. The radiosensitivity of Hep-2 cells transfeeted with or without El A were studied by cell surviral curve. Finally we investigated the correlated mechanisms including cell apoptosis studied by flow cytometry and VEGF content studied by RT-PCR. Resuits The radiosensitivity of Hep-2 cells transfected with El A was intensified, Do and Dq were lowered and α was increased. Flow cytometry showed that the apoptosis rate of cells with E1A or with El A and radiotherapy was increased. The VEGF content of the cells transfeeted with E1 A or treated by radiotherapy was decreased, which reached the lowest level when the cells were treated with the both mathods. Conclusions E1 A gene can intensify the radiosensitivity and contribute to the apoptosis of human laryngeal carcinoma cells. El A gene and radiotherapy can markedly decrease the VEGF content.