0.05).Serum Tg of the seventh day after taking 131 I was remarkably higher than that of the seventh day before taking 131 I ( P 0.05).Conclusions:Treatment of 131 I gives rise to some harm to chromosome of Graves′ disease patients,but the harm can be restored.Serum Tg is related to the frequency of Fra8q22.

Icariin and total flavonoids in "Xianlinggubao Capsule", a Chinese herbal preparationcontaining Epimedium L. as the main ingredient for the treatment of osteoporosis, were determined quan-titatively by HPLC and UV spectrophotometry respectively. It was found that the contents of icariin were4.16～27.67 mg/g and that of flavonoids were 62. 56～109.78 mg/g. The recoveries were 96.17% and103.58% respectively. The method could be used for the quality control of the drug.

The occurrence and development of lung cancer are closely related to gene expression and mutations.Recent studies of lung cancer related genes show that the expression of COPS3 gene may play a key role in the lung cancer cell proliferation.Echinoderm microtubule associated protein like 4-anaplastic lymphoma kinase fusion gene and epidermal growth factor receptor mutations coexist.GA733 gene is closely related to lung cancer survival.Fibroblast growth factor receptor 1,high mobility protein family,members of the scavenger receptor class 5 and epidermal growth factor receptor provide new ideas for targeted therapy of lung cancer.For the therapy of lung cancer,multiple gene therapy and gene therapy combined with conventional treatment is required.

Objective To evaluate the difference between gallbladder polys,gallbladder adenomas and gallbladder cancer by sonography.Methods The imaging features were analysed including 571 patients with gallbladder polys,30 with gallbladder adenomas,109 with gallbladder cancer.Results The lesion diameter,echogram,color signals,maximum velocity(Vmax) and the resistive index(RI) were difference between Polys,Adenomas and cancers of gallbladder.When RI withinlesions lower than 0.65 was adopted as diagnostic criteria of gallbladder cancer,the specificity of the ultra-sound in diagnosis of gallbladder cancer were respectively.Conclusion Color Doppler flow imaging was useful in the differential diagnosis of gallbladder polypoid lesions in combination with gray-scale sonography.

Objective To observe the effect of treating chronic renal failure with self-made Huazhuo Baoyuan decoction.Methods 90 patients of chronic renal failure belonging to spleen and kidney yang deficiency and turbid toxic obstruction type diagnosed by TCM syndrome differentiation were randomly divided into a treatment group and a control group according to the date of reception.40 cases in the control group were treated with conventional western medicine,which were low-salt high-quality low-protein diet; to correct acid-base balance and electrolyte imbalance; to correct anemia; adding amino acids; to control blood sugar in patients with history of diabetes; to control blood pressure in patients with hypertension.50 patients in the treatment group were additionally treated with self-made Huazhuo Baoyuan decoction on the basis of the control group.Such indexes as symptom score,serum creatinine (Scr),blood urea nitrogen (BUN),endogenous creatinine clearance rate (Ccr),hemoglobin (Hb),liver function:aspartate aminotransferase (AST),alanine aminotransferase (ALT) ; lipids:total cholesterol(TC),triglyceride(TG),high density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol (LDL-C) were observed before and after the treatment.Results After treatment,total effective rate was 88.0％ and 67.5％ in the treatment group and the control group respectively,the difference was statistically significant (P=0.012).The value of Scr (292.95± 85.95) μmol/L,BUN (22.69 ± 9.84) mmol/L,Ccr (22.69 ±9.84)rn1/min,CH(6.23 ± 0.57)mmol/L,TG(1.70± 0.22)mmol/L,HDL-C(1.45 ± 0.04)mmol/L,LDL-C(3.30 ±0.60) mmol/L showed significant difference after the treatment than before in the treatment group (P＜0.05 or P＜ 0.01).Compared with the control group of after treatment [Scr (336.63 ± 90.77)μmol/L,BUN(10.47 ±2.71) mmol/L,Ccr (19.47 ± 6.71) rnl/min,CH (7.21 ± 0.62) mmol/L,TG (2.30 ± 0.27) mmol/L,HDL-C (0.87 ±0.05)mmol/L,LDL-C (5.60±0.70)mmol/L],the difference was statistically significant(P＜0.05).Conclusion Self-made Huazhuo Baoyuan decoction was effective and safe to treat chronic renal failure.

ObjectiveTo observe the effects of Shengqing-Jiangzhuo decoction combined with western medicine on metabolic syndrome.Methods120 patients with metabolic syndrome in Gaomi City People's Hospital of Traditional Chinese Medicine from March 2008 to July 2011 were randomly divided into a treatment group and a control group according to case number,with 60 cases in each group.The control group was treated with Western medicine individualized and symptomatic treatment,while on the basis of the control group the treatment group was additionally treated with Shengqing-Jiangzhuo decoction.Both groups were continuously treated for 2 months.The main symptoms of the two groups of patients before and after clinical efficacy as well as blood pressure,body mass index (BMI),waist-hip ratio (WHR) lipid levels,glycosylated hemoglobin (HbA1C) changes were observed.Results① The clinical efficacy:After treatment,dizziness,dry mouth,constipation,bloating,irritability and other symptoms improved significantly,with 85.00％ and 73.33％ of the total effective rate in treatment group and control group respectively; ② laboratory parameters detection:there was statistical difference between the control group and the treatment group in terms of systolic blood pressure (127.30±8.00) mm Hg,diastolic blood pressure (81.35±3.81) mm Hg,body mass index (23.3±1.48),waist-hip ratio (0.85 ± 0.07),as well as cholesterol (3.32 ± 0.81)mmol/L triglyceride (1.76 ±1.01) mmol/L,low-density lipoprotein cholesterol (2.20±0.72)mmol/L,high density lipoprotein cholesterol (1.39±0.14) mmol/L,glycosylated hemoglobin (6.58±0.82) mg/dl,(P＜0.05 or P＜0.01).Conclusion Shengqing-Jiangzhuo decoction significantly improved the clinical efficacy of metabolic syndrome.

Objective To observe the effects of self-made Yicong-Shugan drink in the treatment of anxiety disorders before the college entrance examination.Methods Random number table was adopted for dividing 121 outpatients with anxiety disorders before the college entrance examination in the People's Hospital of Gaomi and Gaomi TCM Hospital since January 2007 to March 2011 into a treatment group with 69 cases,and a control group with 52 cases.The treatment group was treated with Yicong-Shugan Decoction,while the control group was given alprazolam.Hamilton Anxiety Scale (HAMA),the Clinical Global Impression Scale (CGI) and Anxiety Rating Scale (SAS) were used to determine the clinical efficacy in both groups before treatment and 1,2,4,6 weeks after the treatment.Results ① In the control group after treatment,one week HAMA score (23.1 ±7.9) was significantly changed than before the treatment (31.1 ±7.6) (P＜0.05); whereas no significant change in the treatment group (P＜0.05).This demonstrated that alprazolam has a faster affection than Yicong-Shugan drink.② HAMA integral scores,CGI integral scores and SAS scores of both groups at 2,4,6 weeks after treatment were:HAMA integral score:(22.6 ± 7.5),(11.7 ± 5.3),(7.9 ± 5.4) in the treatment group,while (18.7 ±6.9),(13.8 ±6.7),(11.8 ±6.5) in the control group; CGI integral score:(3.4 ±0.7),(2.1 ±0.6),(1.8 ±0.7) in the treatment group,while (3.2 ±0.8),(1.8±0.5),and (1.6 ±0.6) in the control group; the SAS scores:(53.8±8.5),(38.7 ±9.6),(30.6 ±7.6) in the treatment group,while (50.2 ±8.9),(41.5 ±9.8),and (39.2 ±8.3) in the control group respectively.All these scores showed significant difference than those before the treatment in the same group (P＜0.01).HAMA and SAS scores in the treatment group were significantly lower than the control group after the treatment (P＜0.01).This demonstrated that both alprazolam tablets and Yicong-Shugan drink have a good anxiolytic effect,but Yicong-Shuxin drink has a longer lasting effects.Conclusion Self made Yicong-Shugan drink can effectively improve the patient with college entrance examination anxiety disorder.Alprazolam tablets have a quicker effects than self-made Yicong-Shugan decoction,while Yicong-Shuxin decoction has a longer lasting effects than alprazolam.

Objective:To compare the current status of domestic violence(DV)by geographic settings in Hunan province.Methods:Using a multi-stage sampling strategy,3087 households in urban,3070 in rural areas and 3294 in industrial district in Hunan were surveyed.Multiform clue investigation and face-to-face interviews were combined to investigate the current status of DV.Results:The lifetime and 12-month prevalence of spousal abuse,child abuse and elder abuse varied significantly by geographic settings(P

Objective To establish a simple,rapid,accurate and practical method for determination of chloride in drinking water by chloride ion-selective electrode.Methods The chlorine ion-selective electrode was used as indicator electrode and type217saturated calomel electrode of the double salt bridge(the lower salt bridge was filled with a solution of0.1mol/L potassium nitrate)was used as reference electrode.The contents of chloride in water were calculated using the calibration curve method in the electrolyte containing0.1mol/L potassium nitrate.Results The linear range of the method was10 -5 -10 0 mol/L and the best lin ear range was10 -4 -10 -1 mol/L with r=0.9999and the slope=53.5?0.667.The detection limit,recovery rate and coefficient of variation(CV)were0.3545mg /L,94.2%-102.2%(mean98.1%)and2.8%respectively.The suitable pH for the determination of the method was2-10.In the solution containing3.545mg/L chloride,50times more BrO -3 ,NO -2 ,CH 3 COO - ,SO 42- ,F - ,CrO 42- and10times more CO 32- could not interfere with the detection,but Br - and I - were interfering factors.The re-sults obtained by the chlorine ion-selective electrode method and the national standard determination method showed no statistic differences.Conclusion The method was easy to operate,rapid,accurate and suitable for determination of chloride in water.

Objective To study whether intranasally administered transforming growth factor beta1(TGF-?1)can reach central nervous system(CNS)through the trigeminal nerve pathway.Methods Nineteen healthy male SD rats were randomly assigned into control group(n=6)and administration(IN)0.5h group(n=3),1h group(n=4),2h group(n=3)and 6h group(n=3).Rats in IN groups were given 50?l(20?g)recombinant human TGF-?1(rhTGF-?1)intranasally and were sacrificed at 0.5,1,2 or 6h after IN following blood sample collection.The cerebellum,midbrain,pon,medulla and trigeminal nerve were separated quickly and the concentration of TGF-?1 was analyzed by ELISA.Results Compared with the control group(15.01?1.50pg/mg),TGF-?1 was significantly elevated in the trigeminal nerve in all the IN groups(P0.05).TGF-?1 concentrations in plasma and other caudal CNS regions,such as cerebellum,midbrain and cervical spinal cord,were not significantly changed compared with that in control group.Conclusion TGF-?1 is likely to be absorbed by the trigeminal nerve following intranasal administration,and is subsequently delivered to some brain regions through the trigeminal nerve pathway,which may provide a potential treatment strategy for trigeminal nerve and CNS disorders.