Objective To observe the therapeutic effect of acupuncture for treatment of malignant tumor patients with symptoms of digestive system.Methods We carried out a cohort study and enrolled 92 malignant tumor patients hospitalized at Yantai cancer hospital from July 2008 to Jun 2009.They were randomly divided into three groups by the random number table with the ratio of 1 ∶ 1 ∶ 1.The three groups were pattern differentiation acupuncture group that including excess and deficiency syndromes,non pattern differentiation acupuncture group and the western medicine control group.Patients in pattern differentiation acupuncture group accept different acupuncture treatment with different acupoints according to their pattern.Patients in non pattern differentiation acupuncture group only accept acupuncture treatment with fixed acupoints.All patients in the two groups accepted acupuncture treatment one times per day,seven days is a course of treatment.Western medicine control group get routine chemotherapy including CTX,ADM,VCR,VP-16,PDD,MTX 5-FU,and symptomatic and support treatment were added according to the patients symptoms.Efficacy of all groups were evaluated and compared after one course of treatment.Results (1) Comparison of symptom change scores:①Inner group comparison:Patients' symptoms were significantly improved after treatment in pattern differentiation acupuncture group and non pattern differentiation acupuncture group (P＜0.05); The difference of patients' symptoms before and after treatment didn't show statistical significance in western medicine control group differences (P＜0.05).② Comparison among groups:Compared with western medicine control group,the difference among pattern differentiation acupuncture group and non pattern differentiation acupuncture group were statistically significant after treatment (t=2.212,1.989,P＜0.05),the difference between pattern differentiation acupuncture group and non pattern differentiation acupuncture group didn't show statistically significant (t=1.548,P＞0.05).(2) Improvement of digestive system symptoms:Compared with western medicine control group,the difference among pattern differentiation acupuncture group and non pattern differentiation acupuncture group was statistically significant after treatment (x2=15.28,13.45,P＜0.05),the difference between pattern differentiation acupuncture group and non pattern differentiation acupuncture group didn't show statistically significant (x2=9.81,P＞0.05).Conclusion Acupuncture treatment can improve the symptoms of digestive system of malignant tumor patients.

Objective:To establish a specialized detection method for quinolone drugs illegally added in health foods and Chinese traditional patent medicines. Methods: LC-MS/MS was used to simultaneously screen pipemidic acid, norfloxacin, ciprofloxacin, lomefloxacin, enoxacin, fleroxacin, pefloxacin, sparfloxacin, gatifloxacin and ofloxacinan in health foods and Chinese traditional patent medicines. Qualitative and quantitative analysis and methodology validation were studied as well. Results:Under the conditions of the analysis method, the 10 quinolone drugs added illegally obtained a good separation on a chromatographic column. With the two grade mass spectrum for the qualitative analysis and the peak area of multiple reactions monitoring mode for the quantitative calculation, the method was specific and sophisticated. The recovery and linear relationship met the demand of screening. Conclusion:The method is simple and accurate in the detection of quinolone drugs in health food and Chinese traditional patent medicines.

Objective:To determine the contents of paraceltamol and caffeine in New compouad Folium Isatidis Tablets simultaneously by HPLC. Methods:The determination was carried out with C 18 chemical bonded silica gel as a solid phase, methanol-water (25∶75) as a mobile phase and UV deterction wavelength at 215 nm. Results: The average recovenies of the added sample were 99.6%( RSD=0.67, n=5) for paracetamol and 99.3%(RSD=0.58, n=5) for caffine, There was a good linear relationship between the concentration and absorption area value in the rang of 1.6?g～6.4?g for paractamol or 0.16?g～0.64?g for caffeine.Conclusions: The method is simple, quick and accurate.

Objective To explore the correlation between acute gastrointestinal side effects caused by radiotherapy in pancreatic cancer and degree of gastroduodenal radioactive injury under gastoendoscopy.Methods From February 2010 to May 2015,112 patients with pancreatic cancer and received radiotherapy were enrolled.The correlation between gastroscopic findings and degree of gastrointestinal side effects (grade zero to five) was analyzed.The effects of different radiotherapy on the formation of radiation ulcers were also investigated.Chi-square test and Spearman correlation analysis were performed for statistically analysis.Results The incidence of radioactive gastroduodenitis was 57.1 ％ (64/112).The incidence of radiation-induced ulcer was 42.0 ％ (47/112).Among the 112 patients with pancreatic cancer and received radiotherapy,patients with over grade two nausea,vomiting,dyspepsia and constipation received symptomatic treatment.Thirty-one patients (27.7 ％) of them received appetitestimulating treatment,53 patients (47.3％) were given anti-nausea treatment,39 patients (34.8％)received therapy of promoting digestion,and 24 patients (21.4 ％) received therapy of relieving constipation.The incidence of radiation-induced ulcer in patients with grade one acute gastrointestinal side effects was 25.8 ％ (8/31),lower than that of patients with over grade two acute gastrointestinal side effects (48.1％,39/81),and the difference was statistically significant (x2 =4.595,P=0.032).The grade of acute gastrointestinal side effects was positively correlated with that of radiation-induced injury under gastroendoscopy (r=0.295,P =0.002).The incidences of radiation-induced ulcer of gamma knife radiotherapy and helical tomotherapy were 50.0 ％ (15/30) and 39.2 ％ (31/79),respectively,and the difference was not statistically significant (x2 =1.032,P =0.301).Conclusions The occurrence of radiation ulcers should be alerted if over grade two acute gastrointestinal side effects presented in patients with pancreatic cancer and received radiotherapy.The radiotherapy caused gastrointestinal side effects can not be evaluated just according to clinical symptoms.Endoscopic findings and pathological diagnosis are the gold standard.

Objective To analyze the evolutionary trend of clinical features in infective endocarditis.Methods The study enrolled 68 patients who were diagnosed as having infective endocarditis from July 1987 to July 2007.The patients were divided into two groups:group Ⅰ included patients from 1987 to 1997,group Ⅱ included patients from 1997 to 2007.All clinic data were compared.Results In both groups,61 patients(89.7%) had basic heart diseases,67 patients(98.5%) had fever and 62 patients(91.2%)had cardiac murmur;41 patients(60.3%) had positive results in the blood culture.Vegetations were found in 57 cases(83.8%) by echocardiography.The rate of surgical treatment was 19.1%,the total cure rate was 66.7%.Comparing the data between the two groups,age of onset changed from (36.7?12.7)years to (44.4?18.6)years(P

Objective To screen the optimum conditions carrying murine melanoma B16 cells in spaceflights without cares.MethodsMurine melanoma cells were cultured on micocarriers and grouped depending on cells concentration, serum concentration, microcarrier number and temperature.After 33 days, B16 cells were stained by Giemsa, observed with phase-contrast microscope and counted for surviving percentage.ResultsThe optimum conditions,in which the surviving percentages were 8% and 10%, were obtained in the experiments.B16 cells were carried in the 20th recoverable satellite orbiting 18 days under the optimum conditions. After recovering, 110 strain monocloned cells were survived and the surviving percentage was 1.1%.ConclusionThe optimum conditions carrying murine melanoma B16 cells in spaceflights without cares seems to be obtained,and it did improve the surviving time and percentage of cells in spaceflights without cares.

Objective: To evaluate whether restricted breathing movement by wearing elastic chest belt within half a year postoperatively could decrease the incidence of chest wall deformity after costal cartilage harvesting. Methods: The contour of chests of the patients receiving the third-stage operation of auricular reconstruction was assessed 1 year after costal cartilage harvesting with history collection and physical examination. The incidence of costal wall deformity was summarized and compared by wearing elastic chest belt or not within half a year postoperatively. Logistic regression was used to analyze the significance of results. Results: Thirty-eight eligible patients were enrolled in the study. The incidence of chest wall deformity in patients wearing chest elastic chest belt as requested for half a year was lower (64.7% vs. 95.2%), and the difference was statistically significant, OR 10.9, 95%CI[1.16, 102.60], P=0.037. Conclusions Restricted breathing movement by wearing elastic chest belt within half a year postoperatively could decrease the incidence of chest wall deformity following costal cartilage harvesting through decreasing the traction force caused by respiratory muscles and intrathoracic negative pressure.

Objective To explore the differential diagnosis of Meige syndrome (MS) with hemifacial spasm (HFS) to strengthen the degree of recognition of MS for clinical workers.Methods The clinical data of 10 patients with MS,misdiagnosed as HFS and received microvascular decompression (MVD) surgery in other hospitals and admitted to our hospital from May 2005 to November 2016,were retrospectively analyzed.The different differential diagnosis methods and treatment methods were analyzed.Results These 10 patients with MS,who were misdiagnosed as different sides of HFS,took oral drugs,acupuncture therapy,botulinum toxin injection and facial nerve MVD treatments before they went into our hospital.Relapse appeared in these patients at about 2 years.However,in our hospital,these 10 patients were diagnosed as having MS.According to their clinical symptoms,corresponding treatment regimens for symptomatic treatment were given:2 patients underwent deep brain stimulation,and 8 patients with oral medication and psychological and behavioral therapies.The eyelid and lips and involuntary symptoms of convulsions improved significantly.Burke-Fahn-Marsden muscular dystonia rating scale indicated that the improvement rate in the 2 patients accepted deep brain stimulation was 54％-89％,and that in the other 8 patients was 23％-59％.Conclusions MS and HFS have a certain degree of similarity in clinical manifestations,and it is easily confused in the clinical work.But the two are completely different in pathogenesis and treatment,and the wrong treatment may bring disastrous consequences to patients,so it's a great challenge for clinicians to make a clear diagnosis.

The N-terminal of porcine parvovirus (PPV) viral protein 2 (VP2) links a glycine-rich domain which is a cleavage site of PPV VP3.In order to confirm that the glycine-rich domain was essential for the self-assembling of virus-like particles (VLPs).The VP2 gene with glycine-rich domain deleted and the complete VP2 gene were inserted to eukaryotic expression vector pCI-neo and were named pCI-AVP2 and pCI-VP2. Then, pCI-delta VP2, pCI-VP2 and pCI-neo were transferred into Vero Cells by liposome and the VLPs was detected by SDS-PAGE, Western blotting, indirect immunofluorescence and immunoelectron microscopy. Furthermore, 56 female Kunming mice were divided into 5 groups and injected intramuscularly with pCI-delta VP2, pCI-VP2 and pCI-neo as DNA vaccine, PPV inactivated vaccine and normal saline separately. The peripheral blood of the mice was collected to analyze the subgroups of the peripheral blood mononuclear cell by flow cytometry, to detect the antibody and lymphocyte proliferation by indirect-ELISA and MTT assay separately. The results show that the VLPs were observed both in the pCI-delta VP2 and pCI-VP2 transferred Vero Cells. The two VLPs could agglutinate guinea pig erythrocytes. The results also show that both the pCI-delta VP2 and pCI-VP2 vaccine induced special cellular and humoral immunity effectively. Those results revealed that the glycine-rich domain is not essential for the VPL's self-assembling. This study provides a new theoretical evidence for the relationship between the gene structure and protein function of VP2.
Animals ; Antigens, Viral ; genetics ; metabolism ; Capsid Proteins ; genetics ; metabolism ; Cercopithecus aethiops ; Female ; Genetic Vectors ; genetics ; Glycine ; Mice ; Sequence Deletion ; Swine ; Transfection ; Vaccination ; Vaccines, Virus-Like Particle ; biosynthesis ; immunology ; Vero Cells