0.05).The clearance rate of pathogens of teicoplanin was 87.5% and vancomycin was 88.9%.There was no significant difference between them.Both drugs had side effects.The side effects of vancomycin was a little more than teicoplanin.CONCLUSIONS Both vancomycin and teicoplanin are effective drugs in the treatment of G+ cocci in lower respiratory tract infection.The side effects of teicoplanin are smaller than of vancomycin.

Objective To determine the effect of combination of chest physiotherapy (CPT) and intermittent non-invasive ventilation for chronic obstructive pulmonary disease (COPD) patients with respiratory failure. Methods Ninety COPD patients with intermittent bi-level positive airway pressure (BiPAP) ventilation were randomly divided into 2 groups : control group (n=45) received BiPAP treatment after conventional anti-infection, phlegm treatment and support treatment; CPT group (n=45) received CPT before BiPAP treatment. Clinical symptoms, chest signs, chest X-ray, time of BiPAP therapy, PaO2, and PaCO2 after the treatment were evaluated. Results Compared with with the control group, patients in the CPT group significantly improved in clinical symptoms, chest signs, chest X-ray absorption as well as shorter BiPAP therapy time, PaO2 increase and PaCO2 decrease(P<0.05). Conclusion For COPD patients who need intermittent non-invasive BiPAP ventilation, receiving CPT can effectively improve the therapeutic effect. CPT and intermittent non in-vasive BiPAP ventilation cooperate in the treatment of COPD patients with respirstory failure.

OBJECTIVE：To s tudy the protective effect of polydat in complicated with emodin on hyperuricemia （HUA）model rats，and to screen the optimal complication proportion and investigate the potential mechanism. METHODS ：SD rats were randomly divided into normal control group ，model group ，benzbromarone group （positive control ，8 mg/kg），polydatin alone group，emodin alone group and drug combination group A ，B，C，with 5 rats in each group of each dosage. Normal control group and model group were given constant volume of 0.3％ CMC-Na solution ，administration groups were given relevant medicine intragastrically. Each group was given 0.1 mL/10 g intragastrically once a day ，for consecutive 7 d. Expect for normal control group，other groups were given intraperitoneal injection of potassium oxonate 300 mg/kg 1 h before last medication to induce HUA model. One hour after last medication ，the serum contents of uric acid （UA）were determined in normal control group ，model group，benzbromarone group ，polydatin/alone group （0.625，1.25，2.5，5，10 mg/kg）and drug combination group A [the dose of polydatin+emodin were （0.625+0.625），（1.25+1.25），（2.5+ 2.5），（5 + 5），（10 + 10） mg/kg]. The effect （Fa） and combination index （CI） of above single drug groups and combination groups were calculated by the median effect principle. The dose-effect relationship curves of twocomponents alone or combination were drawn ；Fa-CI curves after simulation were als o drawn to evaluate the effect of two-drug combination. Serum contents of UA in rats were determined and Fa value was calculated in single drug groups and drug combination group B ，C [the dose of polydatin+emodin were （0.625+ 0.625），（0.625+1.25），（0.625+2.5），（0.625+5），（0.625+10）mg/kg and （0.625+0.625），（1.25+0.625），（2.5+0.625），（5+ 0.625），（10+0.625）mg/kg]. The optimal complication proportion of two drugs were screened. The serum contents of xanthine oxidase（XOD）in rats were determined in normal control group ，model group ，benzbromarone group ，polydatin/emodin alone group（10 mg/kg）and the optimal complication proportion groups. The mechanism was analyzed primarily. RESULTS ：Compared with normal control group ，the content of UA in model group was increased significantly （P＜0.01）. Compared with model group ， except for 0.625 mg/kg polydatin alone group ，the content of UA in other administration groups were decreased significantly （P＜ 0.05 or P＜0.01）. When the two drugs were used alone or in combination ，Fa value was positively correlated with drug dose （intercept＞0，correlation coefficient ＞0.9），and Fa value of the combination group was higher than that of any single drug group ； when the simulated Fa value was more than 15％，the corresponding CI value was less than 1，two-drug combination showed synergistic effect. When the complication proportion of polydatin and emodin was 1∶4，the Fa value （53.10）was similar to that of drug combination group A （53.73），and the dose of them were less [ （0.625+2.5）mg/（kg·d）vs.（2.5+2.5）mg/（kg·d）]. Compared with normal control group ，serum content of XOD in model group was increased significantly （P＜0.01）；compared with model group，serum content of XOD in administration groups were decreased significantly ，and the optimal complication proportion group was significantly lower than polydatin alone group and emodin alone group （P＜0.05 or P＜0.01）. CONCLUSIONS ：The polydatin and emodin used alone or in combination can reduce the serum content of UA in HUA model rats by inhibiting the generation of XOD. They have a certain synergistic effect ，and the optimal complication proportion is 1∶4.

Objective:To evaluate the diagnostic accuracy of the convolutional neural network（CNN） in diagnosing nasopharyngeal carcinoma using endoscopic narrowband imaging. Methods:A total of 834 cases with nasopharyngeal lesions were collected from the People's Hospital of Guangxi Zhuang Autonomous Region between 2014 and 2016. We trained the DenseNet201 model to classify the endoscopic images, evaluated its performance using the test dataset, and compared the results with those of two independent endoscopic experts. Results:The area under the ROC curve of the CNN in diagnosing nasopharyngeal carcinoma was 0.98. The sensitivity and specificity of the CNN were 91.90% and 94.69%, respectively. The sensitivity of the two expert-based assessment was 92.08% and 91.06%, respectively, and the specificity was 95.58% and 92.79%, respectively. There was no significant difference between the diagnostic accuracy of CNN and the expert-based assessment （P=0.282, P=0.085）. Moreover, there was no significant difference in the accuracy in discriminating early-stage and late-stage nasopharyngeal carcinoma（P=0.382）. The CNN model could rapidly distinguish nasopharyngeal carcinoma from benign lesions, with an image recognition time of 0.1 s/piece. Conclusion:The CNN model can quickly distinguish nasopharyngeal carcinoma from benign nasopharyngeal lesions, which can aid endoscopists in diagnosing nasopharyngeal lesions and reduce the rate of nasopharyngeal biopsy.
Humans ; Nasopharyngeal Carcinoma ; Narrow Band Imaging ; China ; Neural Networks, Computer ; Nasopharyngeal Neoplasms/diagnostic imaging*

Objective: To investigate the differences in frequency and function of natural killer cells (NK) between chronic hepatitis B (CHB) and acute hepatitis B (AHB). Methods: Patients with AHB and those with CHB in immune active (IA) phase were enrolled. The frequencies of NK, CD56^dimNK, CD56^brightNK and the expression of functional molecules IFNAR2 and NKp46 on the surface of NK cells were detected respectively among patients with CHB in IA phase, patients with AHB, and those recovered from AHB. At the same time, their correlations with ALT, HBV DNA and HBV markers were analyzed. Results: Between IA and AHB, the frequencies of NK cells and NKp46^dim NK cells in AHB cases were significantly lower than those in IA cases, but the frequency of NKp46^high NK cells in AHB was higher than that in IA. For patients who recovered from AHB, the frequency of NK cells and NKp46^dim NK cells increased; the varied ranges of frequencies of CD56^dimNK, IFNAR2⁺ NK and NKp46⁺ NK cells were on the rise, while the frequency of NKp46^high NK cells decreased after the recovery from AHB, and the varied ranges of CD56^brightNK and IFNAR2MFI, NKp46MFI decreased. In AHB, HBVDNA loads were positively correlated with ALT levels. Before and after the recovery of AHB: ΔHBV DNA and ΔALT, Δ NK/LY (%) were positively correlated; ΔALT and ΔNKp46^highNK/NK(%), ΔNKp46MFI, ΔIFNAR2MFI were positively correlated. Conclusions In CHB immune active phase, the activity of peripheral blood NK cells was too weak to remove the virus, but NK cells play an important role in eliminating the viruses and mediating liver tissue inflammation in AHB.

Objective: To investigate the differences in function of plasmacytoid dendritic cells (pDC) and CD4⁺ T helper cells (CD4⁺ Th cells) between acute hepatitis B (AHB) and chronic hepatitis B (CHB). Methods: In this study, patients with AHB and those with CHB in immune active (IA) phase were enrolled. The frequencies of pDC, CD86⁺ pDC, CD4⁺ T cells and their subsets, surface functional molecules were detected respectively among patients with chronic HBV infection in IA phase, patients with AHB, those recovered from AHB. Meanwhile, their correlations with ALT, HBV DNA and HBV markers were analyzed. Results: The ALT level in AHB was significantly higher than that in IA, and inflammation was more obvious in AHB. Between IA and AHB, CD86⁺ pDC frequency and the mean fluorescence intensity of functional molecule CD86 (CD86MFI) were higher in IA than those in AHB, but the frequency of CD4⁺ T cells in AHB was higher than that in IA. For patients who got over AHB, the frequency of CD86⁺ pDC increased; Th1 were on the rise, while the frequencies of CD4⁺ T and Th2 decreased after the recovery of AHB, and Th2 / Th1 ratio decreased..In AHB, HBVDNA loads were positively correlated with ALT levels and Th2 frequencies. Conclusions In CHB immune active phase, CD86⁺ pDC with stimulating function played an important role, but the cellular immune response of CD4⁺ T cells decreased. In AHB inflammatory stage, CD4⁺ T cells played a strong cellular immune response, which result ed in viral clearance. Th2 cells regulation of CD4⁺ T cells played a dominant role, which was involved in the inflammatory response, and the cytotoxic role of Th1 cells during the recovery period was dominant, playing a strong cellular immune response, then the virus were completely eliminated.