Animals ; China ; epidemiology ; Rickettsia Infections ; epidemiology ; veterinary ; Rodentia ; Ticks

Objective To study the clinical application of endoscopic retrograde cholangiopancre-atography(ERCP)combined with biliary microlithiasis exploration in acute cholangitis.Methods The clinical data of consecutive 54 cases of acute cholangitis were analyzed.Before ERCP,all cases were examined by routine examinations,including abdominal uhrasonography,CT or magnetic resonance cholangiopancre atography(MRCP).The bile Was collected through ERCP or endoscopic mtsobiliary drainage(ENBD),then all the samples were choked by polarization microscope.Results Thirty-six cases(66.67%)out of 54 patients with common bile duct(CBD)stones or dilation of common bile duct were diagnosed by routine examination.ERCP Was performed in all patients,only 3 cases failed,and the rest were successful.Through ERCP,39 cases with CBD stones(76.47%),4 cases with CBD inflamnmtory smcmre were diagnosed,and 12 cases were normal.Among the 12 cases,9 cages were found with biliary microlithiasis by polarization microscope.The diagnostic effectiveness of ERCP combined with biliary microlithiasis exploration was highest(94.12%),and there Was significant difference between them(P＜0.05).Conclusions It may improve the diagnostic accuracy to combine ERCP with biliary microlithiasis exploration in CBD stones.Biliary microlithiasis may play an important role in the pathogenesis in unknown aetiology acute unexplained cholangitis.

Objective To explore the value of trypsinogen activation pepfide (TAP) levels in blood plasma and urine in the diagnosis of acute pancreafitis at early stage and severity predicting combined with the CT results. Method Sixty-five patients with acute pancreatitis who scanned with enhanced CT were di-vided into mild acute pancreatitis (MAP) group and severe acute pancreatitis (SAP) group according to the CT results, 29 patients with acute abdomen except for acute pancreatitis were as control group. TAP levels in blood plasma and urine were measured in all patients. Results At the time of 6, 12 and 24 h after admis-sion, the level of blood plasma TAP in SAP group ( > 9.0 nmol/L) was significantly higher than that in MAP group(< 3.5 nmol/L), there were significant differences between SAP group and MAP group or control group (P<0.05). And the median urinary TAP in SAP group (102.8, 78.2, 52.3 nmol/L)was significantly higher than those in MAP group (32.5, 28.7, 25.6 nmol/L)and control group(12.6, 12.2, 11.5 nmol/L), there were significant differences among three groups (P<0.05). At the time of 3, 5 d after admission, there was no significant difference in TAP levels in blood plasma and urine among three groups (P>0.05). Conclusions Early dynamic detection of TAP levels in blood plasma and urine is of higher diagnostic value and predictive value for acute pancreatitis, which is of benefit in the early diagnosis and treatment of acute pancreatitis.

Objective·To analyze the clinical characteristics of decompensated cirrhosis patients with intestinal obstruction and related risk factors.Methods·Clinical data of 1 783 decompensated cirrhosis patients treated between March 2010 and March 2016 were collected.Of them,128 (7.18％) patients with intestinal obstruction were screened as the observation group and 128 patients without intestinal obstruction were randomly selected as the control group.Clinical data of two groups were retrospectively investigated,clinical characteristics were compared and analyzed,and related risk factors were analyzed with the Logistic regression analysis.Results·The clinical symptoms of decompensated cirrhosis patients with intestinal obstruction were hidden and misdiagnoses or delayed diagnoses were common.The incidences of abdominal pain,abdominal distension,vomiting,stop exhaust defecate,ascites,electrolyte disorders,fever,and spontaneous peritonitis were significantly higher in the observation group than in the control group (P ＜ 0.05).The results of univariate analysis showed that age,history of abdominal surgery,white blood cell count,serum sodium,serum potassium,neutrophil percentage,and serum albumin were risk factors for decompensated cirrhosis patients with intestinal obstruction.The results of multivariate analysis indicated that age,history of abdominal surgery,white blood cell count,serum sodium,serum potassium,neutrophil percentage,and serum albumin were independent risk factors for decompensated cirrhosis patients with intestinal obstruction.Conclusion·Decompensated cirrhosis patients with age ≥ 50 years old,a history of abdominal surgery,the abdominal cavity infection,low potassium,hyponatremia,and lower serum albumin are likely to develop the intestinal obstruction.

Parkinson's disease (PD) is a complicated disease, commonly diagnosed among the elderly, which leads to degeneration of the central nervous system. It presently lacks an effective therapy for its complex pathogenesis. Adverse effects from Western drug-based medical intervention prevent long-term adherence to these therapies in many patients. Traditional Chinese medicine (TCM) has long been used to improve the treatment of PD by alleviating the toxic and adverse effects of Western drug-based intervention. Therefore, the aim of this study is to evaluate the efficacy and safety of Xifeng Dingchan Pill (XFDCP), a compound traditional Chinese herbal medicine, taken in conjunction with Western medicine in the treatment of PD patients at different stages in the progression of the disease.

Objective To investigate the psychological characteristics and personality of perinatal women, and to provide evidence for effective psychological intervention. Methods Five hundred and seventy pregnant women were randomly selected from the out-patient department from October, 2002 to March, 2003. Eysenck Personality Questionnaire(EPQ) , Self-rating Anxiety Scale(SAS) and Self-rating Depression Scale(SDS) were applied for their personality, psychological characteristics and the influencing factors. Psychological surveys were performed in 216 cases in labor, and 300 cases were followed up during 1-5 days and 42-60 days postpartum. Results During pregnant period, the incidence of anxiety and depression was 10. 5% and 10. 2%, respectively, while 4. 0% and 5. 3% during early stage of puerperium. The rate of depression was 40. 7% and 10. 0% during late stage of puerperium and after puerperium. Pregnant women with personality of stability were less likely to have psychological disorder. Conclusions The rate of psychological disorder varies in different per sonallity. Proper intervention among high risk pregnant women is important in perinatal psychological

BACKGROUND:Eucommia ulmoides gum has good thermoplastics, liquidity, bondability and antimicrobial properties. However, the application of Eucommia ulmoides gum as a root canal filing material is rarely reported. OBJECTIVE: To compare the different contents of gas phase nano-silica on mechanical performance of composite material taking the gutta-percha as the matrix, and observe the effect of the root canal filing. METHODS:Four kind of root canal filing materials were prepared through adding barium sulfate (20%), nano-hydroxyapatite (10%) and different contents of gutta-percha and gas phase nano-silica (40% and 15%, 45% and 20%, 50% and 25%, 55% and 30%). PureEucommia ulmoidesgum served as the blank control. The mechanical properties (hardness, tensile strength and rupture elongation rate) of those five materials were detected. Furthermore, four kinds of composite materials, pureEucommia ulmoidesgum and Gutta-Percha Pelets were filed into the fresh extracted premolar root canal by using Obtura II technology. The effect of root canal filing was observed under X-ray and scanning electron microscopy.RESULTS AND CONCLUSION: Along with the increase of the content of gas phase nano-silica, the hardness of Eucommia ulmoides gum composite material increased, tensile strength increased firstly and then decreased, and rupture elongation rate decreased. PureEucommia ulmoides gum was transmitted to the X-ray and no image of filing material was obtained. Four kinds of composite materials were resistant to X-ray, and images showed that al composite materials were equaly filed into the root canal, without cacuole, and were in contact with the root canal inner wal, showing good filing effect. Scanning electron microscopy showed that, fingerlike projections were the longest at the surface of pureEucommia ulmoides gum; as the content of gas phase nano-silica increased, fingerlike projection length reduced at the surface of composite materials.Eucommia ulmoides gum composite materials could be developed as a canal filing material under heat flow condition.

In order to study the antibacterial activity of Chinese medicinal herbs to Ureaplasma ure- alyticum(Uu),we examined the inhibitory effect of 156 Chinese medicinal herbs to 14 standard strains of Uu by microdilution method in vitro.The results showed that Uu had a higher sensitivity to Cortex phellodendri,Radix angelicae dahuricae,Fructus kochiae,Radix et rhizoma rhei(MIC_(90)≤7.81mg of dried medicinal herbs/ml);a moderate sensitivity to Radix glycyrrrhizae,Radix isatidis,Rhizoma cop- tidis,Herba andrographitis,Herba houttuyniae(15.63≤MIC_(90)≤62.5);a lower sensitivity to Cortex meliae,Herba leonuri(62.5250).It is our opinion that Chinese medicinal herbs are of momentous signfi- cance in the treatment of Uu infection.

Objectives To analyze the characteristic of HIV-1 viral infectivity factor (Vif) gene variants isolated from Shanghai. To construct the prokaryotic expression vector of HIV-1 vif gene and understand its immunogenieity. Methods HIV-1 vii genes were amplified and sequenced from 23 serum samples of HIV-1 infected patients in Shanghai and then compared with the international standard HIV-1 strain. Subsequently, these amplified Vif fragments were sub cloned into pETS2b(+)expression vector. The recombinant prokaryotie plasmids pETS2b (+)-HIV-1/Vif were then transferred into BL21DS(Star)cells for expressing and purifying HIV-1 Vif protein. HIV-1 Viff rat polyclonal antibody was then preparing by injecting the purified Vif proteins into the mice. ELISA was used to determine the purity of Vif proteins and the immunogenicity of its polyclonal antibodies. Results The nucleotide acid mutation rate of HIV-1 vif gene in Shanghai AIDS patient was (0. 179±0. 006)% compared with the international standard HIV-1 strain. Some similar mutations in vif gene were found in HIV-1 strains isolated from Shanghai while the amino acid sequence between 151 and 240 in Vif protein was conserved. The construction of HIV-1 Vif prokaryotic expression plamids and the preparation of Vif polyclonal antibodies were successfully done in this study. The reaction between recombinant HIV-1 Vif protein and the serum from HIV-1 infected patient was not significantly different from that between the recombinant protein and healthy control serum(P＞0.05). HIV-1 Vif polyclonal antibody reacted differently with recombined HIV-1 Vif protein compared with healthy control serum samples (t=178.61, P＜0.01). Conclusions The Vif gene mutation rate is high in HIV-1 strains isolated from Shanghai compared with international standard HIV-1 strain. The prokaryotic expression plasmids of HIV-1 vif antigen are successfully constructed and Vif polyclonal antibodies are prepared well.

Objective Experimental Study of balloon catheter injury technique in establishment of atherosclerosis.Methods The healthy New Zealand rabbits were randomly divided into two groups.(ns=20,weight2.5～3kg,4month old) The two groups were fed a cholesterol-supplemented diet and one group were subject to balloon catheter injury of the abdominalaorta by femoral artery.And three New Zealand rabbits were fed ordinary diet.The abdominal aorta were observed by pathology in both groups in order to determined whether there was atherosclerosis of the rabbit model.The thickness was measured and the quality of plaque was identified.Results Comparing with cholesterol-supplemented diet group,balloon catheter injury group,the typical carotid atherosclerosis was observed.The neoiniimal thickening and plaque were signficant in two groups and the P/IM ratio reached 0.56?9.4 and 0.99?26.9.Conclusion The typical carotid atherosclerosis lesion can induced by cholesterol-diet plus balloon catheter injury of the abdominalaorta in rabbit.