AIM: To explore the effect of ischemic preconditioning on cardiac myocyte apoptosis and the expression of bcl-2 during myocardial ischemia/reperfusion in rats. METHODS: We use TUNEL,immunohistochemical and in situ hybridization(ISH) methods to detect the cardiac myocyte apoptosis and the expression of bcl-2 during myocardial ischemia/reperfusion in rats. RESULTS:① The numbers of positive cardiac myocyte nuclear and the percentage of positive cardiac myocyte nuclear in IP+I/R 3h group decreased significantly(P

AIM: To study the effects of oxidized high-density lipoprotein (oxHDL) on the expression of monocyte chemoattractant protein-1(MCP-1) and intercellular adhesion molecule-1(ICAM-1) and intracellular free calcium concentration ([Ca 2+ ]i) level in cultured human umbilical venous endothelial cells(HUVECs). METHODS: The MCP-1 protein content in the medium of conditioned HUVEC was measured by ELISA, and the ICAM-1 on HUVECs was detected by indirect immunofluorescence, and [Ca 2+ ]i was determined by Fluo-3/AM, the injury of cells was observed by scanning electron microscopy (SEM).RESULTS: oxHDL could induce the expression of MCP-1 and ICAM-1 in HUVECs. In oxHDL group (HUVECs were incubated with 100 mg protein/L oxHDL for 24 h), the levels of MCP-1, ICAM-1 and [Ca 2+ ]i increased by 160%, 60% and 70% respectively compared with the control group ( P

AIM: To explore the effect of PKC activition on cardiac myocyte apoptosis and expression of bcl-2 during myocardial ischemia/reperfusion(I/R) in rats. METHODS: TUNEL,immunohistochemistry and in situ hybridization were used. RESULTS: The TUNEL data showed that the numbers of positive cardiac myocyte nucleus and the percentage of positive cardiac myocyte nucleus in PMA+IR 3 h group decreased significantly( P

AIM: To explore the effect of brief mesenteric artery occlusion (MAO) on cardiac myocyte apoptosis and bcl-2 expression during myocardial ischemia/reperfusion (I/R) in rats. METHODS: TUNEL,immunohistochemical and in situ hybridization(ISH) methods were applied in the present study. RESULTS: ① The numbers of positive cardiac myocyte nuclear and the percentage of positive cardiac myocyte nuclear in MAO+IR 3h group decreased significantly ( P

BackgroundNon-suicidal self-injury （NSSI） behavior has become a major public health concern and can have significant implications for the physical and mental health of adolescents. Peer victimization is a risk factor for adolesents to have NSSI behavior， so exploring the relationship and underlying mechanism between peer victimization and NSSI functions will provide a promising strategy for the prevention and intervention of NSSI behavior. ObjectiveTo investigate the relationship between peer victimization and NSSI functions in adolescents with unipolar and bipolar depression， so as to provide references for the intervention of NSSI behavior in adolescent patients with unipolar and bipolar depression. MethodsUsing multi-stage stratified sampling， 940 adolescents with unipolar and bipolar depression who met the Diagnostic and Statistical Manual of Mental Disorders， fifth edition （DSM-5） criteria for bipolar depressive episodes or depressive disorders were selected from 14 psychiatric hospitals in China. All participations were assessed using Chinese version of the Functional Assessment of Self-Mutilation （C-FASM）， Multidimensional Peer-Victimization Scale （MPVS）， UCLA Loneliness Scale （UCLA-LS） and Patient Health Questionnaire-9 item （PHQ-9）. Pearson correlation coefficient was to assess the correlation among above scales， and the model fit and path coefficients for mediation were analyzed with model 4 in Process 4.0 for SPSS. ResultsA total of 698 （74.26%） adolescents with unipolar and bipolar depression completed the questionnaire survey. NSSI behavior was detected in 374 patients （53.58%）. Among adolescents with unipolar and bipolar depression and NSSI behavior， MPVS total score was positively correlated with the scores of NSSI emotion regulation function， attention-seeking function and social avoidance function in C-FASM （r=0.104， 0.130， 0.266， P<0.05 or 0.01）， UCLA-LS score also yielded a positive correlation with the scores of NSSI emotion regulation function， attention-seeking function and social avoidance function in C-FASM （r=0.321， 0.112， 0.246， P<0.05 or 0.01）， and UCLA-LS score was positively correlated with MPVS total score （r=0.241， P<0.01）. Loneliness demonstrated a complete mediating role in the relationship between peer victimization and emotion regulation function， with an indirect effect value of 0.033 （95% CI： 0.019~0.050） and an effect size of 73.33%. A partial mediating effect of loneliness was also observed for the relationship between peer victimization and social avoidance function， with an indirect effect value of 0.016 （95% CI： 0.007~0.025） and an effect size of 17.98%. ConclusionLoneliness may act as a mediator in the relationship between the peer victimization and the NISS emotion regulation and social avoidance functions in adolescents with unipolar and bipolar depression and NSSI behaviors. ［Funded by Guangzhou High-level Clinical Key Specialty （number， SZGSP013）］

Objective: To investigate the clinical manifestations, pathological features, and treatment of oral and maxillofacial pyogenic granulomas induced by camrelizumab. Methods: A case of pyogenic granuloma of the gums and lips caused by camrelizumab was reported along with a literature review. Results: After 4 months of treatment with camrelizumab for liver cancer, the patient developed systemic reactive capillary hyperplasia (RCH), followed by multiple masses on the lower lip and gingiva. After periodontal therapy, the masses on the lower lip and the gingiva were removed, and camrelizumab administration was stopped. The pathological result was gingival pyogenic granuloma/granulomatous hemangioma. No new masses were found in the oral cavity during postoperative follow-up. A review of the literature showed that RCH is the most common adverse drug reaction to camrelizumab but it occurs infrequently in the oral cavity. At present, the etiology of RCH has not been clarified, but the research has shown that camrelizumab may trigger tissue proliferation into hemangiomas by activating vascular endothelial cells, and the combined use of camrelizumab is safer than single use. RCH is self-limiting and most cases resolve spontaneously after discontinuation of the drug. If the mass causes dysfunction, surgical excision is feasible. Conclusion Camrelizumab can cause oral and maxillofacial reactive capillary hyperplasia complicated by pyogenic granuloma.