Objective To evaluate the effects of application of MgCI2, ATP, and ATP-MgCl2 on the funcrions of liver, kidney, heart and lung in experimontelly ischemic injuried animals, and to study the therapeutic role of exogenous Mg2+ and energy on repairmat of this injuny ming. Freeze etching, Histochemistry, Atomic Absorhtion Spectrum (AAS) and combine LM and EM. The experiment showed that MgCl2 and ATP protectived functions damnify organic (P>0.01) ,ATP-MgCl2 protected effect it notable results (P<0.01 ). Cell membrance was complete, glycogen became enriched in the cytoplasm, the uniform-distributed protein granule on Pside of plasma membtance of cell was found. There were positive material in the membrances and similar to normal group, cell michondia calcium decreased. Conclusions The exogenous application of ATP-MgCl was beneficial to the critical patient.

Objective To investigate the clinical significance of protein expression of Six1 in cervical cancer. Meth?ods The immunohistochemical (IHC) staining was applied to detect the expression of Six1 protein in normal cervical tis?sues (n=32), cervical intraepithelial neoplasia (CIN) tissues (n=49) and cervical cancer tissues (n=123). The localization of Six1 protein was detected in vitro cultured HeLa cells using immunofluorescence (IF) staining. Results The positive rate of Six1was significantly higher in cervical cancer (72.3%) than that of CIN tissues (28.6%) and normal cervical tissues (15.6%,χ2=13.118 and 10.058 respectively, P<0.01). There were significance differences in expression levels of Six1 protein be?tween different tumor sizes and metastasis of cervical cancer (P < 0.01). The Six1 protein showed positive signals in cyto?plasm and nucleoli in HeLa cells. Conclusion Six1 expression is associated with cervical cancer, which may be a potential biomarker for invasion and metastasis of cervical cancer.