Objective:To establish a method for qualitative and quantitative analysis of the major active components in sepia.Methods: Melanin was identified by dissoving it in a basic solution to see whether there was segregation of a black substance.Glycine,asparagines,glutamic acid and alanine in sepia were determined by HPLC-AccQ-Tag method and iron was determined by atomic absorption spectrophotometer.Results: Melanin in sepia was dissolved in 2 mol/L NaOH solution and black substance was obtained in 2 mol/L HCl solution.The recoveries of the above 4 amino acids and iron were within the range of 95%-105% with RSD

To discuss the therapeutic effect and influence of rat NGF on serum neuropeptide Y ( NPY) and neuron-specificenolase (NSE) levels in newborn with neonatal hypoxic and ischemic encephalopathy (HIE). Methods: Totally 70 cases of neonatal HIE patients were selected and divided into the observation group and the control group at random. The children in the two groups were given the routine medical treatment such as oxygen uptake, the control of intracranial pressure, blood pressure and blood sugar, anticonvulsant, balance of water and electrolyte and etc. The children in the observation group were additionally given 20μg rat NGF through intramuscular injection once a day. The children in the control group were additionally given 100 mg citicoline through in-travenous drip once a day. The treatment course was 10-14 days. The changes of serum NPY and NSE levels, clinical curative effect and adverse drug reactions ( ADR) in the two groups were compared, and the occurrence of nervous system sequel after the medical treatment was compared and followed up. Results: After the 2-week medical treatment, the serum NPY and NSE levels in the two groups were declined (P<0. 05 or P<0. 01), and the declining in the observation group was more notable than that in the control group (P<0. 05). The total clinical efficiency in the observation group was 94. 28%, which was much higher than that (68. 57%) in the control group (P<0. 01). No obvious adverse drug reaction appeared in the two groups during the treatment. After the one-year follow up, the occurrence of sequel was 5. 71% in the observation group, which was much lower than that (22. 86%) in the control group(P<0. 05). Conclusion:Rat NGF has reliable curative effect on neonatal HIE with high security, which can improve the repair of impaired neuronal cells, reduce the occurrence of sequel of neuronal cells and enhance the prognosis, and the mechanism may relat-ed with the reduction of serum NPY and NSE levels.

Idiopathic thrombocytopenic purpura (ITP),also called immune thrombocytopenia more frequently by the international,is the most common acquired hemorrhagic disease characterized by thrombocytopenia,presence of anti-platelet antibody in the plasma,with increased or normal megakaryocytes with abnormal maturation.About 70％ of 1TP patients recover spontaneously within 6 months,while a small proportion of patients will transform to persistent,chronic or refractory idiopathic thrombocytopenic purpura.New immunosuppressive agents and thrombopoietin for the treatment of children with refractory ITP have been developed.Dramaric progresses in the diagnostis,severity assessment and treatment of refractory ITP have been made recently,which is comprehensively illustrated in this review.

The incidence of gastrointestinal diseases increases every year.Etiology and pathogenesis of gastrointestinal diseases have been not yet elucidated,such as inflammatory bowel disease,gastrointestinal cancers,and allergic diseases.In recent years,the role of mucin genes expression in gastrointestinal diseases has been paying much attention to.Studies have shown that mucins are the main component of mucus in the digestive tract,and a major factor in the formation of the gastrointestinal barrier.Mucins are related to gastrointestinal epithelium,intestinal microflora and inflammatory factors.Abnormal expression of gastrointestinal mucin genes,especially MUC1 and MUC2,are related to Gastrointestinal inflammation and cancer.Currently gastrointestinal mucin genes expression contribute to exploring the diagnosis,treatment and prognosis of digestive diseases.This study is to summarize the progress in gastrointestinal mucin genes expression.

Objective:To apply positive composite training on gynecologic tumor patients for psychological intervention,comparing with the general intervention,and to provide a new way to alleviate physical and psychological symptoms of patients and improve their quality of life.Methods:In Beijing Cancer Hospital,80 surgery and chemotherapy patients with gynecologic tumor were chosen and divided into control group (40 cases,to receive health education and supportive treatment) and intervention group (40 cases,to receive comprehensive training),whose condition and basic situation were matching.After intervention,the scores of Symptom Checklist 90 (SCL-90) and Quality of Life Questionnaire- Core 30 (QLQ-C30) were compared between the two groups.Results:After intervention,the SCL-90 scores in intervention group (outside terrorist and interpersonal sensitivity factor) were lower than that in control group[such as total score,(1.43±0.34) vs.(1.87±0.43),P<0.001].The scores of physical function,role function,emotional function,cognitive function,and general health in the QLQ-C30 about patients with chemotherapy in intervention group were higher than those in controls[such as general health,(62.50±10.29) vs.(52.50±15.78),P=0.024].And the scores of fatigue,nausea and vomiting,pain,insomnia,appetite loss,constipation,and diarrhea factors were lower than those in control group[such as fatigue,(52.77±16.07) vs.(65.55±20.36),P=0.034].Conclusion:The positive composite training is able to alleviate the physical and mental symptoms and improve their quality of life in gynecologic tumor patients.

Objective To investigate the status of diabetes distress in patients with middle-andhigh risk of diabetic foot and explore the impact of self-perceived burden and social support on diabetes distress.Methods Totally 205 patients with middle-and-high risk of diabetic foot were investigated with Diabetes Distress Scale,Self-perceived Burden Scale and Social Support Scale.Results The total average score of diabetes distress was (2.59±0.57),the diabetes distress level was correlated with self-perceived burden,dimensions of social support (r=0.481～0.653) and were influenced by gender,vocation,objective support,use of support.Conclusions Diabetes distress is widespread in patients with middleand-high risk of diabetic foot and was influenced by many factors,these provide the basis for taking effective interventions to reduce diabetes distress.

Objective To investigate the effect of propofol preconditioning on activities of ATPase on myocardial cell membrane in rats with myocardial ischemia-reperfusion injury. Methods Forty Sprague-Dawiey rats were subject to 30 min ischemia and 60 min reperfusion and randomly assigned to five groups, six rats per group: control(C) group; ischernia-reperfusion (IR) group; propofol preconditioning group(P, according to the dosage of propofol, P was assigned to three KM+-ATPase and Ca2+-Mg2+-ATPase of left ventricle myocardial tissue were measured by chemical colorirnetric method. The rnyocardium pathomorphism was observed with light microscopes. Results The activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in ischemia-reperfusion group were lower than control group and propofol preconditioning group(P<0. 05 or P<0.01). Conclusion Propofol preconditioning can produced cardioprotective effect against ischemia-reperfusion injury by recovering the ATPase activities of myocardial tissue.

Cancer and stroke are the major diseases causing serious harm to human health.Studies show that as compared with normal people,patients with cancer are more prone to stroke.We reviewed the research progress on cancer patients with stroke within the past decade,including the common cancer type which causes stroke,the stroke pathogenesis of cancer patients,clinical characteristics,treatment and prognosis of cancer patients with comorbid stroke,in order to provide the evidence for the prevention and treatment of stroke in cancer patients.

Orbital metastases rarely occur in the patients with cancer,especially from a primary pancreat-ic carcinoma.In patients presenting ophthalmologic symptoms such as local pain and periorbital edema,it is im-portant to consider orbital metastatic disease.And it is also crucial to determine the site of the primary tumor for better treatments.The mechanism underlying the orbital metastasis from pancreatic carcinoma remains to be clari-fied.In this report,we describe the clinical characteristics of a case with orbital and hepatic metastases from pri-mary pancreatic carcinoma,providing a reference for clinical diagnosis.Computed tomography of the abdomen and left orbit,in combination with intraorbital biopsy,suggesting a primary pancreatic malignancy with metastatic dis-ease.Surgical pathology and histopathological examination results confirmed the diagnosis.After single agent gem-citabine chemotherapy,the patient′s symptoms relieved.

The article reviews the development of patterns of stroke unit, the roles of different types of stroke unit, the mechanisms of stroke unit in improving prognosis, and the prospect of stroke unit.