Objective To study the diagnostic value of CDE in moyamoya disease.Methods The intra-and-extracranial arteries in 17 patients with moyamoya disease and 25 healthy volunteers were studies by CDE.The resistance index(RI),pulsatility index(PI),time-averaged flow velocity(Vm)were examined in arteries,including MCA、MCA、ACA、PCA、CCA、ICA、ECA and TSA.Results ①The diagnosis rate of the occlusion or stenosis in intracranial arteries was 87.25%.②The hemodynamic characteristics of CCA and ICA were high flow velocky and low resistance opposite to that of TSA.③The moyamoya vessels were seaned in 10 of all 17 patients with moyamoya disease by CDE.The diagnosis rate of moyamoya disease was 58.82%.Conclusions CDE technique could image the moyamoya vessels as scattered color dots. So CDE had a higher clinic value in diagnosis of moyamoya disease.

With the exploitation of marine resources and environmental deterioration, perilous events in the sea are gradually increasing. Seawater drowning is one of the most important causes of death. Researches on seawater drowning have attracted more and more interests in recent years, and certain progresses have been achieved in scientific research and clinical treatment. In the aspects of theory and establishment of a model, a renewed concept of seawater drowning-induced acute lung injury (SWD-ALI) has been acquired, the animal model of SWD-ALI has been successfully reprodaced for elucidation of the pathological and pathophysiological changes in lung tissue. Multiple results of molecular biological research on SWD-ALI have contributed to illuminating the pathogenesis involved. It is indicated that, compared with freshwater-induced lung injuries, SWD-ALI is severer in clinical picture, and it might develop to a peculiar seawater drowning-induced acute respiratory distress syndrome (SWD-ARDS) if no prompt and effective treatment is employed. For the clinical treatment for SWD-ALI, comprehensive therapies based on mechanical ventilation have been approved. The scientific outcomes mentioned above have laid a foundation for the full realization of SWD-ALI and more effective rescue of SWD-ALI patients.

Objective To observe the effect of partial liquid ventilation (PLV) and conventional mechanical ventilation(CMV) on gas exchange in lung injury due to pulmonary edema as a result of seawater drowning in rabbit. Methods Twenty-four healthy male new Zealand rabbits were randomly divided into three groups ( n =8). Pulmonary edema was produced in all rabbits by drowning in sea water. Rabbits of PE group received no treatment,that of CMV group were treated with conventional mechanical ventilation and PEEP,and that of PLV group with CMV,combined with PEEP and FDC. All rabbits were treated for 3 hours. Blood gases,TNF-?in plasma and pulmonary histopathology were determined during the experiment. Results In PLV group,PaO_2 was increased obviously,inflammatory exudate was reduced,and alveolar and interstitial edema was alleviated. The plasma level of TNF-?was significantly lower in PLA group compared with PE group,( P

Objective To review the clinical features of lung nodule and the common misdiagnosed reasons and diseases. Methods A total of 44 literature on misdiagnosed lung nodule in the latest ten years were reviewed and their clinical characters, diagnosis methods were analyzed. Results Forty-four relative literatures were found, in which there were 319 misdiagnosed cases. The median of onset age was 43 years. Female patients were more than male. Cough, chest distress and fever were primary symptoms. 46. 39% of the patients were characterized with hilus of lung lymphadenectasis symmetrically. The most common misdiagnosis were tuberculosis, lung cancer and lymphadenoma ( 53.61%, 25. 71% and 10. 34% ). Majority of patients were correctly diagnosed by biopsy, especially through transbronchial lung or mucosa biopsy or lymphaden biopsy. Conclusion Clinicians should improve the understanding on lung nodule and comprehensively analyze and differentiate it with other diseases to reduce the misdiagnosis.

Objective　To investigate regularity of intimal-medial　thickness（IMT）of extracoronary artery (including carotid, femoral and common iliac arteries ) in patients with coronary artery disease (CAD).Methods　Atherosclerosis of carotid, femoral and common iliac arteries was investigated with ultrasound in 115 subjects undergone coronary arteriography (CAG). Stepwise multiple regression analysis and Logistic regression were carried out between the results of extracoronary artery ultrasound examination and CAG.Results　The IMT and incidence of carotid, common iliac and femoral atherosclerostic plaques in patients with positive CAG were significantly higher than the subjects with negative CAG (P<0.01); Logistic regression indicated that the presence or absence of carotid, common iliac, femoral atherosclerosis was closely correlated with CAD (β were 2.147, 2.813 and 3.204 respectively,P<0.05,<0.01,<0.005 respectively. Odds ratio were 8.797,16.666 and 24.642 respectively,P<0.001). Stepwise multiple regression analysis showed that common iliac and femoral IMT were closely associated with LOG (1+Gensini′s score) (P<0.0001). For each increased 1 mm of common iliac and femoral IMT, the LOG (1+Gensini′s score) increased 0.20 and 0.19 respectively. Furthermore, the carotid artery IMT was also partly associated with LOG (1+Gensini′s score) (P<0.05). Common iliac atherosclerosis was closely correlated with occurrence of acute myocardial infarction,the positive predictive value of carotid, common iliac, femoral atherosclerosis was 75%, 87%, 88% respectively, the positive predictive values for 2 sites and 3 sites were 92%～98% and 100% respectively.Conclusions　The presence or absence and the extent and severity of coronary artery atherosclerosis can be predicted through investigating extracoronary artery atherosclerosis.Common iliac and femoral arteries can be asurrogate and window for studying the occurrence, progression of coronary artery atherosclerosis.

Objective To assess the effects of survivin-specific siRNA on chemosensitivity of lung cancer cell A549 to paclitaxel in vitro.Methods For determining if survivin-specific siRNA could enhance the responsiveness of lung cancer to paclitaxel,human lung adenocarcinoma cell lines A549(p53 wild-type)were divided into four different treatment groups:control,survivin siRNA,paclitaxel and survivin siRNA+paclitaxel.The effects on cell proliferation,cell cycle and apoptosis were analyzed by MTT assay and flow cytometry,respectively.The protein expression levels of survivin,p21 and PARP were evaluated by Western blot experiments.Results Survivin-specific siRNA showed a augmenting effect on the chemosensitivity of different concentrations of paclitaxel(P

Objective To explore the difference of invovling rate in intracranial arteries and hemodynamics in extracranial arteries between child group and adult group moyamoya disease.Methods In order to differentiate the child and adult group moyamoya disease,the hemodynamic parameters such as pulse index(PI), resistance index(RI), mean velocity(Vm),systolic velocity/distolic velocity(S/D) were studied by ultrasound and the involving rate of intercranial arteries were studied by digital subtraction angiography.Results ① The involving rate of posterior cerebral artery(PCA) in child group was signifiicantly higher than that in adult group,but no difference in middle cerbral artery(MCA) and anterior cerebral artery(ACA); ② The mean flow velocity of vertebra artery(VA) were significantly reduced but RI,PI,S/D increased in child group than that in adult group moyamoya disease.No difference existed in other extracranial arteries.Conclusions The difference of extracranial arteries hemodynamics owes to the difference of intracranial artery involving rate.

Objective Through transmission electron microsco pe to observe the ultrastructural changes in external iliac arteries of hyperhomocysteinemia rabbits with endothelial dysfunction.Methods Eighteen rabbits were randomized into two groups (n=9 per group).Control group (N group) was poured water into stomach,Methionine group (M group) were poured L-methionine ( 5.0 g/kg weight) into stomach.Endothelium-dependent dilation (EDD),endothelium-independent dilation (EID) were examined by high resolution ultrasound at 0,4,8,12,24 h,at same time and external iliac arteries were observed by transmission electron microscope after 24 h.Results ① In acute hyperhomocysteinemia,EDD of external iliac artery in M group was time-dependent reduced more significantly than that in N group,but EID was not affected. ② Under transmission electron microscope,external iliac arteries of rabbits showed endothelial cells dropping,mitochondrion swelling in endothelial cells and smooth muscle cells,internal elastic lamina disruption,migration of smooth muscle cell to subendothelial space.Conclusions In hyperhomocysteinemia,homocysteine could lead to endothelial dysfunction in rabbits and ultrastructural damage of endothelial cells,smooth muscle cells and internal elastic lamina.High resolution ultrasound could find the endothelial dysfunction accurately.

Objective To analyze the flow spectra of in ternal jugular vein (IJV) in healthy adults by pulsed Doppler ultrasonography. Methods IJV flow velocities in 120 healthy volunteers were recorded during cardiac and respiratory cycles respectively with the transducer placed in cervical region view. Results In healthy subjects, IJV waveforms were composed of one reverse wave A and two forward waves S and D during cardiac cycle. The flow velocities of wave S [( 27.17 ? 19.88 )cm/s vs ( 19.04 ? 14.26 )cm/s] and wave D [( 17.43 ? 12.78 )cm/s vs ( 12.70 ? 10.57 )cm/s] were quicker during inspiration than those during expiration (P 0.05 ]. The velocities of wave S and wave D in the group aged over sixty years old were lower than those in the group aged between 20-60 years old(P