MDM2-binding protein(MTBP)factor is a binding protein of MDM2,and MDM2 gene and involved in the development of cancer as an important gene ,which has been studied for a long time .It has been demonstrated that MTBP could regulate biological processes such as metabolism ,growth,and apoptosis of cells .In the past few years ,some studies have found that MTBP is down -regulated in variety of malignancies ,which is as-sociated with the progression and the prognosis of these tumors .MTBP may be a potential target for the treatment of many kinds of malignant tumors .This paper reviews the research pregressof MDM 2-binding protein on canc-er.

Objective To explore the clinical application value of combined detection of serum brain natriuretic peptide (BNP),homocysteine(HCY) and blood lipids in the patients with heart failure.Methods The levels of serum BNP,HCY,TC,TG,HDL-C,LDL-C,ApoA,ApoB and Lpa were detected in 100 patients with heart failure (observation group) and contemporaneous 100 persons (control group) undergoing healthy physical examination.Then the comparative analysis was performed.Results Compared with the control group,the BNP and HCY levels in the observation group were significantly increased(P＜0.05);among 7 indicators of the blood lipid,the Lpa level in the observation group was increased compared with the control group,while the TC,HDL-C and LDL-C levels were significantly decreased(P＜0.05),and the TG,ApoA and ApoB levels had no statistical differences between the two groups(P＞0.05).Conclusion Serum BNP,HCY,TC,HDL-C,LDL-C and Lpa levels have close correlation with heart failure,especially the combined detection of BNP,HCY and Lpa,which has predictive and diagnostic value in heart failure and is worthy of clinical popularization and application.

Objective To systematically evaluate the type and magnitude of effectiveness of pulmonary rehabilitation program on patients diagnosed with chronic obstructive pulmonary disease (COPD) in none-acute phases.Methods Two investigators independently searched three databases including Cochrane library,Pub Med and CNKI to collect randomized controlled trials about Pulmonary Rehabilitation Program on COPD Patients.A total of 17 trials were included.The quality of these RCTs was evaluated by two investigators and the combination of study results was conducted using RevMan 5.0.2 software.Results Pulmonary Rehabilitation Program could improve COPD patients' exercise tolerance,quality of life,lung function and blood oxygen level.It was also beneficial to patients' discomforts including dyspnea and fatigue.Conclusions The meta-analysis had identified a significant effect of pulmonary rehabilitation program on COPD patients,so nurses are suggested to integrate effective programs into clinical practice.

Influenced by a variety of pathologic factors, cardiac fibroblasts can transdifferentiate into myofibroblasts and pro-liferate excessively, followed by an overdue secretion of extracellular matrix ( ECM) .This process involves complicated signaling path-ways and electrophysiological mechanisms and may result in atrial fibrosis and contribute to atrial remodeling in structure, function and electrophysiological signaling, which is considered as a most important phase of atrial fibrillation.This review focuses on the role of fi-broblasts in atrial fibrosis, atrial remodeling, and the development of atrial fibrillation.

Objective Oridonin is a poorly water soluble drug with low bioavailability.The study aimed to prepared improve the dissolution rate of oridonin by preparation of oridonin solid dispersion with poloxamer. Methods The oridonin solid dispersion was prepared by dissolvent method with poloxamer188 as the carrier and determined by DSC and powder X-ray diffraction.The dissolu-tion rates in different pH dissolution mediums of pure drug, physical mixture and solid dispersion were determined by HPLC method. Results Oridonin exists as an amorphous state in the solid dispersion.Compared with the pure drug and physical mixture, the orido-nin solid dispersion was improved greatly without the pH influence on the drug release.The dissolution rate of oridonin solid dispersion in water, pH 6.8 PBS and pH 1.2 HCl were (92.6 ±4.2)%, (93.1 ±3.5)%and (94.4 ±2.9)% respectively. Conclusion Taking inpoloxamer188 as the carrier, the oridonin solid dispersion has successfully developed its dissolution rate.

Objective To investigate the preventive effect of fibroblast growth factor on infection after tooth extraction among type 2 diabetes mellitus patients. Methods 76 patients with infection prevention were treated with basic fibroblast growth factor, and 90 patients in the control group(n= 324). The intervention group was treated with basic fibroblast growth factor and treated with recombinant bovine basic fibroblast growth factor gel. The routine treatment group was treated with routine rinsing liquid for disinfection to prevent infection. Follow-up observation of infection after 1 week. Results In the group of basic fibroblast growth factor, the infectious rate was 2.63%, which was not significantly lower than that of the control group. The level of white blood cells in the group treated with basic fibroblast factor was (6.67±1.08)×109/L, which was significantly lower than that of the control group. The CRP level was (90.33±12.95) mg/L, which was significantly lower than that of the control group Group level (P<0.05). The incidence of fibroblast infection was 2.7% in men and 6.38% in control group. Although there was no significant difference between the two groups, the difference in the total WBC count and CRP level between the two groups was significant different (P<0.05). The group which use of basic fibroblast growth factor, the total white blood cell count and CRP level of the infection prevention intervention group were significantly lower than the control group (P<0.05). Similar results were found in women. Conclusion Fibroblast growth factor can be used to prevent postoperative infection, and achieve satisfactory results.

Objective:To establish a method of inducing dendritic cells(DC)from the hematopoitic stem cells of rats in vitro,and to identify the phenotype and fusion of DC. Methods:DC obtained from SD rat bone marrow hematopoitic stem cells were propagated in vitro under the condition of rGM CSF、rIL 4 and nrhTNF ?.DC were purified by monoclonal antibody OX62 and magnetic beads.Then DC harvested 12 d later were identified by morphological features,surface antigen expressions and the ability to atimulate T cells. Results:After culture and induction,DC displayed typical morphology with elongated dendritic processes viewed by inversion microscope as well as electron microscope.DC expressed high level surface antigens,including OX62 62.19%;MHCⅠ 70.40%;MHCⅡ 78.28%;CD80 55.58%; CD86 68.38%, The results of mixed lymphocyte reaction(MLR)showed that DC had the ability to stimulate vigorous proliferation of allo T cells. Conclusion:Matue DC could be generated from rat bone marrow hematopoitic stem cells,which presents the feasibility for further clinical application of DC in the immunotherapy of cancers.

Objective To observe the changes of cervix in women between 16 and 35 weeks of gestation using transvaginal ultrasound and to determine the value of the cervix length in predicting preterm delivery among the women with threatened preterm labor Methods Transvaginal sonography were performed in 154 normal single nulliparous pregnant women between 16 and 35 weeks of gestation and 58 women with threatened preterm labor but without premature rupture of membrane Results (1) There is no definitely change in either cervical length or internal diameter of cervix among the pregnant women over the studied period There was also no funneling to be observed The mean cervical length is (36?5) mm,and the mean cervical internal diameter is (4?1) mm (2) Eleven preterm deliveries occured among the 58 pregnancy women with threatened preterm labor The cervical length by sonography of 11 preterm deliveries was (18?6) mm,and was significant shorter than the women without preterm deliveries whose mean cervical length was (32?6) mm ( P

BACKGROUND: Mesenchymal stem cells (MSCs) are capable of differentiating into a variety of lineages, including bone, cartilage, or fat, depending on the inducing stimuli, specific growth and differentiation factors.Recombinant human bone morphogenetic protein 2 (rhBMP-2) produced by gene engineering has an obvious osteoinductive activity and can induce undifferentiated mesenchymal cells into cartilage and bone irreversibly, resulting in new bone formation. Basic fibroblast growth factor (bFGF) modulates chondrogenic and osteogenic differentiation of MSCs.OBJECTIVE: To investigate the effect of bFGF and rhBMP-2 on the differentiation and proliferation of cultured rabbit mesenchymal stem cell in order to find out an optimal way of osteogenesis instead of conventional osteogenic supplements (OS).DESIGN: A randomized and controlled trial.SETTING: General Institute of Orthopaedic Oncology, Tangdu Hospital,Fourth Military Medical University of Chinese PLA.MATERIALS: The subjects were rabbit mesenchymal stem cells cultured by the author.METHODS: This experiment was conducted at the Center of Orthopaedic Surgery, General Institute of Orthopaedic Oncology, Tangdu Hospital,Fourth Military Medical University of Chinese PLA from June 2004 to December 2004. ①Rabbit MSCs cultured in vitro were treated with different growth factor (100 μg/L rhBMP-2, 100 μg/L bFGF, 10 μg/L rhBMP-2and 100 μg/L bFGF and OS; ②The proliferation and differentiation of MSCs were observed through activity of MTT, expression of alkaline phosphatase(ALP), and von Kossa staining.MAIN OUTCOME MEASURES: the rate of proliferation and the activity of ALP.RESULTS: ①rhBMP-2 could promote the proliferation and differentiation of MSCs, especially the cell differentiation; ②bFGF could stimulate the proliferation , the cellular proliferation rate increased 100% as compared with control group, and has no effect on differentiation of MSCs , but it could enhance effect on the cell proliferation of rhBMP-2.CONCLUSION: bFGF and rhBMP-2 are effective induction factors for MSCs. Both of them can stimulate the proliferation and differentiation of MSCs in vitro. bFGF and rhBMP-2 exerted a synergetic action in speeding up the pace of osteoinduction and osteogenesis and can be used to differentiate seed cells for tissue engineering bone.

ObjectiveTo analyze the MRI features of aggressive fibromatosis (AF) in order to improve its diagnostic accuracy.Method The clinical files and MRI appearances of 66 AF patients (primary 19 cases,recurrent 47 cases) were reviewed and compared with the postoperative pathological findings.ResultsThe median age of all patients was 31 years( range,11—60 years) with a male-to-female sex ratio of 1 ∶ 3.4.Eighty tumors were discovered.There were 5 superficial fibromatosis and 75 deep fibromatosis in which 2 lesions were intraabdominal,6 lesions in the abdominal wall and 67 lesions extraabdominal.The average long diameter of all lesions was ( 8.7 ± 5.4 ) cm,of superficial lesions ( 5.7 ±2.8) cm,of deep lesions ( 8.9 ± 5.5 ) cm.Of the 80 tumors,79 were displayed as space-occupying intramuscular lesions; 47(58.8％ ) were ovoid or lobulated and 22( 27.5％ ) were infiltrative in shape; 48 (60％) lesions had a well-defined margin,of which 4 formed a pseudocapsule as they enlarged by compressing normal tissue.To compare with the muscle signal intensity on MRI,75 lesions demonstrated isointensity,mild hyperintensity or hypointensity on T1 WI,heterogeneous high intensity on T2 WI,and avid heterogeneous enhancement after contrast administration.There was no necrosis or surrounding edema in all lesions.Tumors destroyed bone in 2 cases.ConclusionAggressive fibromatosis has characteristic features on MRI,and MRI is valuable in diagnosising AF and evaluating the extend of lesion and involvement of adjacent structures.