Objective:To estimate the effect of treatment opportunity for severe caries in primary molars on child dental fear and dent-al behavior.Methods:288 children aged 3 -6 years with primary molars caries were included on the first visit.The children in the ex-perimental group(n =144),with obvious clinical symptom,were initiative to see a dentist.Those in control group(n =144)without evident symptom were found with caries in conventional dental examination.The change of the children fear before and after the first treatment was assessed by MCDASf within the first week,the change of children's clinical behavior in their first and return visit was esti-mated by Venham Clinical Anxiety and Cooperation Behavior Measurement Scale.Data was statistically analyzed.Results:After the first treatment and during the return visit,the MCDASf average score of the experimental group was decreased(P <0.05),that of the control group was not(P >0.05).On their return visit,the behavior of the 2 groups was similar (P >0.05),and which was ameliora-ted in the experimental group during their own first and return visit,compared with the control group(P <0.05).Conclusion:Treat-ment of severe caries before acute symptom arising in young children may alleviatie the child dental fear.

OBJECTIVETo observe the clinical efficacy of BO's abdominal acupuncture for obese type-2 diabetes mellitus (T2DM).

METHODSSixty patients of obese T2DM were randomly divided into an acupuncture group and a medication group, 30 cases in each one. Patients in the medication group were treated with basic treatment combined with oral administration of regular antidiabetics, three weeks as one session. Patients in the acupuncture group, based on the medication group, were treated with abdominal acupuncture at Yinqiguiyuan [Zhongwan (CV 12), Xiawan (CV 10), Qihai (CV 6), Guanguan (CV 4)], Fusiguan [Huaroumen (ST 24), Wailing (TE 5)], Tianshu (ST 25), Daheng (SP 15), Qixue (KI 13), etc.; the treatment was given three times per week, 3 weeks as one session. The systolic blood pressure (SBP), diastolic blood pressure (DBP), body weight, waist circumference (WC), hip circumference, body mass index (BI) were observed before and after treatment in the two groups, and fasting plasma glucose (FPG), fasting insulin (FINS), 2-hours postprandial blood glucose by oral glucose tolerance test (OGTT) and insulin, total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C), HOMA-IR of insulin resistance index were calculated and adverge events were recorded.

RESULTSCompared before the treatment, SBP, WC, body weight, BMI, FPG, OG-TT2hBG, FINS, GTT2h insulin, HOMA-IR, TC and LDL-C in the acupuncture group were all significantly reduced (all P <0. 05), while FPG, OGTT2H insulin and TG were increased in the medication group (all P<0. 05)'. The differences of reducing SBP, WC, FPG, OGTT2H insulin, HOMA-IR, TC, TG and LDL-C were statistically significant between the two groups (all P<0. 05). The total effective rate was 93. 3% (28/30) in the acupuncture group, which was significantly superior to 23. 3% (7/30) in the medication group (P<0. 01).

CONCLUSIONBO's abdominal acupuncture has obvious clinical efficacy for obese type-2 diabetes mellitus, featuring in lowering blood pressure, reducing weight, decreasing blood glucose, im- proving insulin resistance and lowering lipid, which has no adverse effects and is worthy of clinical popularization and application.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Blood Glucose ; metabolism ; Body Mass Index ; Body Weight ; Cholesterol ; blood ; Diabetes Mellitus, Type 2 ; metabolism ; physiopathology ; therapy ; Female ; Humans ; Insulin ; blood ; metabolism ; Lipoproteins, LDL ; blood ; metabolism ; Male ; Middle Aged ; Triglycerides ; blood ; Waist Circumference

Objective To evaluate the value of anti-carbamylated protein (CarP) antibody in the diagnosis of rheumatoid arthritis (RA).Methods The clinical data and serum of 247 in-patients with joint pain of Department of Rheumatology,General Hospital of Ningxia Medical University admitted during September 1,2015 to June 1,2016 were collected and divided into two groups according to the final diagnosis:RA group (case group) which included 126 patients and the non-RA group which included 121 patients (control group).The concentration of anti-CarP antibody in serum was measured by enzyme linked immunosorbent assay (ELISA).The serum rheumatoid factor (RF) and anti-cyclic citrullinated peptide (CCP) antibodies were detected at the same time.Mann-Whitney U nonparametric test for statistical analysis,receiver operating characteristic curve (ROC) was drawn to determine the cut-off value of anti-CarP antibody to RA diagnosis and to analyze its diagnostic efficacy.Results The ROC curve showed that the anti-CarP antibody had a cutoff value of 63.77 U/ml,with the sensitivity of 74.60％,and specificity of 71.07％.Compared with RF and anti-CCP antibody,the area under the ROC curve (A) were:Aanti-cyclic citrullinted peptide(anti-CCP)antibody (0.88)＞ARF (0.85)＞Anti-CarP antibody (0.78).There was no significant difference between the Aanti-CCP antibodies and ARF as well as ARF and Aanti-CarP antibodies (Z=1.29,P=0.20;Z=1.30,P=0.20),but there was significant difference between Aanti-CCP antibody and A anti Carp antibody (Z=2.28,P=0.02).Conclusion Anti-CarP antibody has certain value in the diagnosis of RA,and can be used as the serological index for RA diagnosis.

Objective To investigate the changes of urine microalbuminuria(UMA) and serum C‐reactive protein(CRP) levels in patients with diabetic retinopathy(DR) and its significance .Methods A total of 152 type 2 diabetes mellitus(T2DM ) patients were included and 40 healthy individuals as normal controls(NC) .T2DM patients were divided into 3 groups ,65 patients with non‐dia‐betic retinopathy(NDR group) ,53 patients with non‐proliferative diabetic retinopathy(NPDR group) and 34 patients with prolifera‐tive diabetic retinopathy(PDR group) .UMA ,CRP and other clinical parameters were detect .Results The level of UMA and CRP increased progressively from NC ,NDR ,NPDR ,to PDR groups ,and there were significant differences among groups(P<0 .05) .The analysis of spearman rank correlation showed that UMA was positively associated with CRP(r=0 .311 ,P<0 .05) .UMA was also positively associated with the duration of diabetes ,fasting blood glucose(FBG) ,low density lipoprotein cholesterol(LDL‐C) ,hemo‐globin A1c(HbA1c) and homeostasis model assessment of insulin resistance(P< 0 .05) ,and CRP was also positively associated with the duration of diabetes ,FBG ,HbA1c and HOMA‐IR(P<0 .05) .Conclusion The development of DR is closely associated with kidney injury ,and chronic inflammation ,hyperglycemia and insulin resistance may be involved in the development and patho‐genesis of DR by interacting the renal function of T2DM patients .

Purpose To detect the expression of FOXO3 in lung cancer specimens and lung cancer stem cell, for diagnosis and treat-ment of lung cancer. Methods Immunohistochemistry, semi-quantitative PCR and Western blot were used to detect FOXO3 protein expression in clinical specimens of lung cancer. PCR and Western blot verified FOXO3 mRNA and protein expression of lung cancer cells. Results Immunohistochemistry showed that the FOXO3 was significantly lower expressed in lung cancer tissues and the corre-sponding lung cancer stem cells;Detection of FOXO3 mRNA and protein by RT-PCR and Western blot showed both had significantly lower expression;as progression of the disease, there was a clear decreasing trend, in which negative expression rate of FOXO3 protein accounted for 66. 7% in all patients;at the same times, the rate of the expression gradually decreased with loss of differentiation and metastasis of the tumor. Conclusions FOXO3 shows low expression in lung tissue and lung cancer stem cells, while it is closely relat-ed to the degree of malignancy and lung cancer metastasis, which may be a new target for the treatment.

Objective To understand the making of laws and regulations for banning smoking in the public places in big cities in China and to provide the reference for making a national code.Methods The methods of literature search and review were employed,as well as questionnaire survey.Results 45.7% of the big cities had the relevant local laws and(or)regulations and 10.4% of the codes were issued by the local People's Congress,1.3% by the other institutions.Definitions used in the local legal and regulatory codes are different.Of the local legal and regulatory codes,17.4% involved four to six types of the public places,68.7% involved seven to eight types and 13.9% involved nine to ten types.Among the codes,8.6% provided that smoke ban inspector should be appointed by the authority in charge,31.9% required the department where smoking is banned to appoint the inspector,58.6% did not request the appointment at all.As for the violating law unit,9.6% of the codes issued a fine of 5-500 Yuan,20.0% issued a fine of 500-1 000 Yuan,10.4% issued a fine of 1 000-2 000 Yuan and 9.6% issued a fine of 2 000-5 000 Yuan.As for the violating law individual,12.1% of the codes issued a 1-5 Yuan fine,22.4% issued a 5-10 Yuan and 10.3% issued a 20-100 Yuan.In 5.8% of the big cities,the codes were revised.Conclusion Up to now,there is no a national law for banning smoking in the public places in China.The effect of the local laws and regulations on banning smoking in the public places is not satisfactory.China still has a long way to go in controlling smoking in the public places compared with the advanced countries.

s teaching was also significantly higher in the study group than in the control group(97.37% vs.64.71%,P＜0.05).Conclusion Micro-teaching can effectively improve the quality of clinical teaching.

Objective To analyze the risk factors of esophageal and gastric variceal bleeding (EGVB) in patients with cirrhosis.Methods A retrospective study was conducted in 638 hospitalized patients with cirrhosis from 2002 to 2009,who were divided into study group as having EGVB (n =286) and control group as not having EGVB (n =352).Differences between 2 groups were analyzed with univariate analysis and multivariate logistic regression.Results Child-pugh classification,serum albumin,prothrombin time,portal vein diameter and spleen thickness were significantly different between 2 groups (P ＜ 0.05).Univariate analysis showed that serum albumin(OR =0.944,P =0.000),prothrombin time (OR =1.067,P =0.007),portal vein diameter (OR =3.423,P =0.007) and spleen thickness (OR =1.276,P =0.007) were correlated with EGVB.Multivariate logistic regression analysis showed that serum albumin (OR =0.936,P =0.000),portal vein diameter (OR =4.098,P =0.013) and spleen thickness (OR =1.275,P =0.000) were independent risk factors for EGVB in patients with cirrhosis.Conclusion Low serum albumin level,increased portal vein diameter and spleen thickness are the risk factors for EGVB in patients with cirrhosis,which can be important predictors.To some extent,increasing serum albumin might reduce the risk of EGVB.

To develop a method for the distinction of genuine Gastrodia elata B1. from its four falsified plants of common occurrence and determination of its active principle, gastrodin by capillary electrophoresis. Methods　The electrophoretic conditions were a buffer solution containing 24 mmol/L borate (pH=9.4), at a voltage of 16 kV. Results　The electrophorograms obtained under the above conditions showed easily distinguishable differences. Baseline separation of gastrodin was achieved and its quantitative analysis can be accomplished within 15 min. The linear correlation equation was Y=-0.048+7.79X (r=0.999). Conclusion　This method could serve as an easy and precise method for the quality control of G. elata.

Object To develop a method for the distinction of genuine Gastrodia elata B1 from its four falsified plants of common occurrence and determination of its active principle, gastrodin by capillary electrophoresis Methods The electrophoretic conditions were a buffer solution containing 24 mmol/L borate (pH=9 4), at a voltage of 16 kV Results The electrophorograms obtained under the above conditions showed easily distinguishable differences Baseline separation of gastrodin was achieved and its quantitative analysis can be accomplished within 15 min The linear correlation equation was Y=-0 048+7 79X (r=0 999) Conclusion This method could serve as an easy and precise method for the quality control of G elata