Objective To study the benzofuran compounds from roots and rhizomes of Ligularia caloxantha, which is a folk medicine used in the Naxi Nationality in Yunnan Province. Methods Compounds were separated and purified by silica gel and Sephadex LH-20 column chromatography. Their structures were elucidated by physicochemical properties and spectral analysis. Results Eighit compounds are isolated from ethanolic extracts of the roots and rhizomes. They were identified as euparin (Ⅰ), 6-methoxy-euparin (Ⅱ), 4, 5-dimethoxy-2-hydroxybenzaldehyde (Ⅲ), 2-acetyl-5, 6-dimethyoxybenzofuran (Ⅳ), 4-hydroxyacetophenone (Ⅴ), 2-isopropenyl-5, 6-dimethoxy-2, 3-hydrocumaran (Ⅵ), 8?-hydroxy-7(11)-eremophilen-12, 8?-olide (Ⅶ), lupeol (Ⅷ). Conclusion All the eight compounds were obtained from L. caloxantha for the first time as benzofurans. Compounds Ⅰ and Ⅱ are two major ones with insecticide and insect food refusal-induced activities. That is the relative reason of L. caloxantha used for folk anti-insect.

Objective To study the protein binding rate of glaucocalyxin A in plasma of rat. Methods The plasma balance dialysis was used. An HPLC for the quantitative determination of glaucocalyxin A was presented and used to calculate the protein binding rate in plasma of rat. Results There were protein binding rate results of 74.46%, 77.87%, and 75.29% at three various concentrations 20, 10, and 1 ?g/ mL of glaucocalyxin A in plasma of rat. Conclusion The HPLC method used to determine glaucocalyxin A is simple, rapid, and sensitive with good specificity, precision and accuracy, and glaucocalyxin A has medium capacities in protein binding rate in plasma of rat.

AIM: To study the effects of Radix Saposhnikoviae(RL) and Feutus Trbuli(FI) used singly and combinedly on antagonizing type Ⅰ allergy,such as passive cutaneous anaphylaxis(PCA),released model from mast cell granules,Schulz-Dale respond. METHODS: Mouse and rat models mainly induced by IgE were used in experiments. RESULTS: RL and FI used singly and combinedly could significantly inhibit PCA of same species of mice,the degranulation of mast cells of skull in rats and Schulz-Dale response. CONCLUSION: Both RL and FI respectively have antagonistic effect on type Ⅰ allergy and RL in combination with FI will possess more satisactory effect

Object To perform detail studies on different species of Ligusticum L (Uumbelliferae) currently prescribing in Yunnan Province for the purpose to clarify the claotic state of its clinical use Methods By literature retrieval, field and market center inspection and pharmacognostic identification in comparison with those documented in classical literatures to arrive at a reasonable evaluation Results Two varieties of Ligusticum L., the Huanggaoben and Heigaoben were currently in common use in Yunnan Province Huanggaoben, the yellow ligusticum, was originated from Sinodielsia yunnanensis Wolff and Ligusticum delavayi Franch Heigaoben, the balck ligusticum, was oringinated from Ligusticum pteridophyllum Franch Conclusion The study may provide references for the further study and clinical use of this medicinal plant

Object To study the chemical constituents of Hedysarum sikkimense Benth. ex Baker var rigidum Hand Mazz Methods The constituents were repeatedly separated and purified on silica gel column They were identified and structurally elucidated by physicochemical data and spectral analysis Results Nine compounds were obtained Eight of them were lupeol (Ⅰ); isoformononetein (Ⅱ); 3 (4 hydroxyphenyl) 6,7 dimethoxy 4H 1 benzopyran 4 one (Ⅲ); ursolic acid (Ⅳ); docosanoic acid 2,3 dihydroxypropyl ester (Ⅴ); lignoceric acid (Ⅵ); ? sitosterol (Ⅶ) and sucrose (Ⅷ) Conclusion All of them are isolated from this plant for the first time

Objective: To study the pharmacological effects of Radix Saposhnikoviae (RL) and Fruetus Tribuli (FI) used singly and combinedly. Methods: The effect of the tested drugs on the animal itching models induced by dextran 40 and Histanmine, skin capillary permeability induced by Histamine and experimental nettle rash induced by dimethylsulfoxide (DMSO) were observed. Results: RL and FI used singly and combinedly could obviously relieve the itch, inhibit the increase of skin capillary permeability induced by Histamine and resist experimental nettle rash induced by dimethylsulfoxide (DMSO) in different degree. Conclusion:RL and FI used singly and combinedly have anti allergic effect and the effect of RL and FI used combinedly is the same as used respectively.